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The map label for this gene is mrdA [H]

Identifier: 150008495

GI number: 150008495

Start: 2236835

End: 2238703

Strand: Reverse

Name: mrdA [H]

Synonym: BDI_1881

Alternate gene names: 150008495

Gene position: 2238703-2236835 (Counterclockwise)

Preceding gene: 150008496

Following gene: 150008494

Centisome position: 46.53

GC content: 47.99

Gene sequence:

>1869_bases
GTGGAGAATAATACCAAGTATGAGTTTGAGAACAGGCGCTATGTCTTAGGTGGGGCGGTGATCCTGTTGGTTATCATCTT
TATTATCCGTTTGTTCATCCTTCAGGTGGTCGACAACGATTATAAAGCTTGGGCGGATAGTAACGCTTTCTTGAAAAAGA
CCTTATATCCTTCCCGAGGCATGATGTACGACCGTAATGGTAAATTATTGGTCTATAACCAGCCCGCTTACGACGTGATG
CTTATCATGCGTGAGATACAGCCTTTCGATACCCTCGATTTCTGCGATGTGCTGGGCATCACGAAAGAGCAATTCCTCAA
ACGTATAGCGGATATTAAGAACCGGCGGTTAAATCCCGGTTATTCTTCCTATGTCCCTCAAGTATTTATGAACCAGCTTT
CCGCGCAGGAATATGGCGTGTTGCAGGAGAAGTTATACAAATTTCCCGGCTTCTATATACAGAATCGCACGATCCGCCAA
TACGAGTATCCCAATGCCGCTCTTGTGCTGGGAAATATCGGCGAGGTAAACCGGAAAGATATAGAGAGTGACTCTTATTA
TACACAAGGCGATTACTCCGGCAGGACCGGGATCGAACAATCGTACGAGGAATATTTGCGGGGCGTGAAGGGTGTCGAGA
TCCTGTTGCGTGACGCTCACGGACGTATCAAGGGGCGATACGAGGAAGGCCTGCACGATATCCAGCCGGAATCCGGCAAA
AACTTGAAATTATCCTTGGATATGGACTTGCAGGCTTATGGGGAGAAGTTAATGCAGAATAAATTAGGGGGTATCGTGAT
GATAGAGCCGGAGACAGGGGAGGTGCTTTGTATGGTTTCCGCCCCTACGTATGATCCGAGTATCTTGATCGGGCGCCAGC
GGGGAAAGAACTACGTGGCTTTGCAGAAGGACCCGTTGAAACCTCTGTTCAACCGTCCGCTCATGGCGCAATATCCTCCC
GGATCTACTTTTAAACCGACCCAAGGTTTGATCTTCTTGCAAGAAGGTGTGATTACCCCGGAAACTCAATATACCTGTGC
GCATGGATACACCTTCCGGGGTGGTAAGCCGGCTTGCCACGGGCATCCGTCTCCTCTGAATCTGGTCGGGGCGTTGGCGA
CCTCTTGTAACTCTTTTTTCCCTTGGGGACTGCATGATATGATCGACAGCCGGAAGCGATATCCCTCGGTACAGGAGGCT
TTTGAGGTTTGGAAGAATCACATGGTCTCAATGGGATATGGCTATAAGTTGGGAATTGATCTTCCGGGAGAGAAGCGAGG
GTTCATTCCGAATAGTAAGTTCTACGATAAGGTGTACCGGGGACGTTGGAATTCCAGTACGGTTATCTCGATCGCTATCG
GTCAGGGTGAGATATTGGCTACGCCTTTGCAAATCTGTAATTTGGCGGCTACTGTCGCTAATCGGGGATATTATATTACT
CCGCATGTCGTAAAAGAGATTCAAGACACTCCGCTGGATACATTATATAGTAAGAGGCATTATACATCGGTGGATACGCA
TTATTATAGTTATATTGCCGAGGGTATGCGAGGGGCTGTTACGGGAACCGCTTATGGTGGAACCTGTCGTGGGGCGGCTT
TACCGGATATCGAGGTTTGTGGAAAGACGGGAACGTCGGAGAATCCGCATGGTAAGGACCATTCTATTTTTATGGGATTC
GCTCCTTATCAAAAGCCGAAGGTCGCCATTGCCGTGTTTGTGGAAAACGCCGGATTCGGTGCTACCTATGCGGTGCCTAT
CGGTAAATTGATGTTGGAGAAATATTTGAAAGGAGAAATATCCGAGGCTAACAAGGCGACGGAGGAATATATTATGAATG
CCGTAATACTACCGAACAATGCGCTATAG

Upstream 100 bases:

>100_bases
TTTGACCCGTTGTTTCTGGTTGTCCGTATCCTCTCGAGCGTGGTGATGACAACCTTATTGATTTGTACGGTAGAAGCATT
CAATATAGAGTCTCAGAAAA

Downstream 100 bases:

>100_bases
GAAAACGGAAATATGGAAGTCGGTAGACTGGATTACCATTTTGTTGTATTTGATTATGGTAATCGCCGGTTGGTTTAGTA
TTTGTGGGGCGAGCTACGAG

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MENNTKYEFENRRYVLGGAVILLVIIFIIRLFILQVVDNDYKAWADSNAFLKKTLYPSRGMMYDRNGKLLVYNQPAYDVM
LIMREIQPFDTLDFCDVLGITKEQFLKRIADIKNRRLNPGYSSYVPQVFMNQLSAQEYGVLQEKLYKFPGFYIQNRTIRQ
YEYPNAALVLGNIGEVNRKDIESDSYYTQGDYSGRTGIEQSYEEYLRGVKGVEILLRDAHGRIKGRYEEGLHDIQPESGK
NLKLSLDMDLQAYGEKLMQNKLGGIVMIEPETGEVLCMVSAPTYDPSILIGRQRGKNYVALQKDPLKPLFNRPLMAQYPP
GSTFKPTQGLIFLQEGVITPETQYTCAHGYTFRGGKPACHGHPSPLNLVGALATSCNSFFPWGLHDMIDSRKRYPSVQEA
FEVWKNHMVSMGYGYKLGIDLPGEKRGFIPNSKFYDKVYRGRWNSSTVISIAIGQGEILATPLQICNLAATVANRGYYIT
PHVVKEIQDTPLDTLYSKRHYTSVDTHYYSYIAEGMRGAVTGTAYGGTCRGAALPDIEVCGKTGTSENPHGKDHSIFMGF
APYQKPKVAIAVFVENAGFGATYAVPIGKLMLEKYLKGEISEANKATEEYIMNAVILPNNAL

Sequences:

>Translated_622_residues
MENNTKYEFENRRYVLGGAVILLVIIFIIRLFILQVVDNDYKAWADSNAFLKKTLYPSRGMMYDRNGKLLVYNQPAYDVM
LIMREIQPFDTLDFCDVLGITKEQFLKRIADIKNRRLNPGYSSYVPQVFMNQLSAQEYGVLQEKLYKFPGFYIQNRTIRQ
YEYPNAALVLGNIGEVNRKDIESDSYYTQGDYSGRTGIEQSYEEYLRGVKGVEILLRDAHGRIKGRYEEGLHDIQPESGK
NLKLSLDMDLQAYGEKLMQNKLGGIVMIEPETGEVLCMVSAPTYDPSILIGRQRGKNYVALQKDPLKPLFNRPLMAQYPP
GSTFKPTQGLIFLQEGVITPETQYTCAHGYTFRGGKPACHGHPSPLNLVGALATSCNSFFPWGLHDMIDSRKRYPSVQEA
FEVWKNHMVSMGYGYKLGIDLPGEKRGFIPNSKFYDKVYRGRWNSSTVISIAIGQGEILATPLQICNLAATVANRGYYIT
PHVVKEIQDTPLDTLYSKRHYTSVDTHYYSYIAEGMRGAVTGTAYGGTCRGAALPDIEVCGKTGTSENPHGKDHSIFMGF
APYQKPKVAIAVFVENAGFGATYAVPIGKLMLEKYLKGEISEANKATEEYIMNAVILPNNAL
>Mature_622_residues
MENNTKYEFENRRYVLGGAVILLVIIFIIRLFILQVVDNDYKAWADSNAFLKKTLYPSRGMMYDRNGKLLVYNQPAYDVM
LIMREIQPFDTLDFCDVLGITKEQFLKRIADIKNRRLNPGYSSYVPQVFMNQLSAQEYGVLQEKLYKFPGFYIQNRTIRQ
YEYPNAALVLGNIGEVNRKDIESDSYYTQGDYSGRTGIEQSYEEYLRGVKGVEILLRDAHGRIKGRYEEGLHDIQPESGK
NLKLSLDMDLQAYGEKLMQNKLGGIVMIEPETGEVLCMVSAPTYDPSILIGRQRGKNYVALQKDPLKPLFNRPLMAQYPP
GSTFKPTQGLIFLQEGVITPETQYTCAHGYTFRGGKPACHGHPSPLNLVGALATSCNSFFPWGLHDMIDSRKRYPSVQEA
FEVWKNHMVSMGYGYKLGIDLPGEKRGFIPNSKFYDKVYRGRWNSSTVISIAIGQGEILATPLQICNLAATVANRGYYIT
PHVVKEIQDTPLDTLYSKRHYTSVDTHYYSYIAEGMRGAVTGTAYGGTCRGAALPDIEVCGKTGTSENPHGKDHSIFMGF
APYQKPKVAIAVFVENAGFGATYAVPIGKLMLEKYLKGEISEANKATEEYIMNAVILPNNAL

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=620, Percent_Identity=29.3548387096774, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1786272, Length=622, Percent_Identity=24.5980707395498, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 70024; Mature: 70024

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNTKYEFENRRYVLGGAVILLVIIFIIRLFILQVVDNDYKAWADSNAFLKKTLYPSRG
CCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCCCCCC
MMYDRNGKLLVYNQPAYDVMLIMREIQPFDTLDFCDVLGITKEQFLKRIADIKNRRLNPG
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
YSSYVPQVFMNQLSAQEYGVLQEKLYKFPGFYIQNRTIRQYEYPNAALVLGNIGEVNRKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCEEEEECCCCCCCCCC
IESDSYYTQGDYSGRTGIEQSYEEYLRGVKGVEILLRDAHGRIKGRYEEGLHDIQPESGK
CCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCHHCCCCCCC
NLKLSLDMDLQAYGEKLMQNKLGGIVMIEPETGEVLCMVSAPTYDPSILIGRQRGKNYVA
EEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCEEE
LQKDPLKPLFNRPLMAQYPPGSTFKPTQGLIFLQEGVITPETQYTCAHGYTFRGGKPACH
EECCCCCHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEECCEEECCCCCCCC
GHPSPLNLVGALATSCNSFFPWGLHDMIDSRKRYPSVQEAFEVWKNHMVSMGYGYKLGID
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEEEE
LPGEKRGFIPNSKFYDKVYRGRWNSSTVISIAIGQGEILATPLQICNLAATVANRGYYIT
CCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCEEECHHHHHHHHHHHHCCCEEEC
PHVVKEIQDTPLDTLYSKRHYTSVDTHYYSYIAEGMRGAVTGTAYGGTCRGAALPDIEVC
HHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHC
GKTGTSENPHGKDHSIFMGFAPYQKPKVAIAVFVENAGFGATYAVPIGKLMLEKYLKGEI
CCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCH
SEANKATEEYIMNAVILPNNAL
HHHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MENNTKYEFENRRYVLGGAVILLVIIFIIRLFILQVVDNDYKAWADSNAFLKKTLYPSRG
CCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHCCCCCC
MMYDRNGKLLVYNQPAYDVMLIMREIQPFDTLDFCDVLGITKEQFLKRIADIKNRRLNPG
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
YSSYVPQVFMNQLSAQEYGVLQEKLYKFPGFYIQNRTIRQYEYPNAALVLGNIGEVNRKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEEECCCEEEEECCCCCCCCCC
IESDSYYTQGDYSGRTGIEQSYEEYLRGVKGVEILLRDAHGRIKGRYEEGLHDIQPESGK
CCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHCCHHCCCCCCC
NLKLSLDMDLQAYGEKLMQNKLGGIVMIEPETGEVLCMVSAPTYDPSILIGRQRGKNYVA
EEEEEECCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCCCCCEEEEECCCCCCEEE
LQKDPLKPLFNRPLMAQYPPGSTFKPTQGLIFLQEGVITPETQYTCAHGYTFRGGKPACH
EECCCCCHHHCCCCEEECCCCCCCCCCCCEEEEECCCCCCCCCEEEECCEEECCCCCCCC
GHPSPLNLVGALATSCNSFFPWGLHDMIDSRKRYPSVQEAFEVWKNHMVSMGYGYKLGID
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEEEE
LPGEKRGFIPNSKFYDKVYRGRWNSSTVISIAIGQGEILATPLQICNLAATVANRGYYIT
CCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCEEECHHHHHHHHHHHHCCCEEEC
PHVVKEIQDTPLDTLYSKRHYTSVDTHYYSYIAEGMRGAVTGTAYGGTCRGAALPDIEVC
HHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHC
GKTGTSENPHGKDHSIFMGFAPYQKPKVAIAVFVENAGFGATYAVPIGKLMLEKYLKGEI
CCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHCCCH
SEANKATEEYIMNAVILPNNAL
HHHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]