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The map label for this gene is wcaJ [H]

Identifier: 150008370

GI number: 150008370

Start: 2067486

End: 2068886

Strand: Reverse

Name: wcaJ [H]

Synonym: BDI_1744

Alternate gene names: 150008370

Gene position: 2068886-2067486 (Counterclockwise)

Preceding gene: 150008371

Following gene: 150008369

Centisome position: 43.0

GC content: 46.97

Gene sequence:

>1401_bases
ATGAAGAAAAGCGTACAAGCCGGAAAATATATTGTCTCGGATTTTATAGCGTCGTCGGTAGTCTGGTTATTATTTAACCT
GATACGCTATGAGGAGGTGGCGGTGTATGAAGGGTTTGATACCGTCGGGGCCTTTTTGCTGCATATCCCCTCATTGAAGG
GGCAGCTATTGATCCCGTTTTTCTGGTTCGTATTATATTGGTTTTCCGGTTATTATAATAAACCGTTCGGTAAGTCGAGG
CTTACTGAGTTGTTCTCTACCCTTGGGAGCGTGTCGATCGGTGTCGTGATCTTGTTTTTTGCCTTAGTGCTGAATGACTT
GCCCCGTTCTTATCACGTGTATTATGATATGTTTTTCTCTTTATGGGGATTGCAGTTTGTCTTGACGTATATTCCCCGGT
TATTGATTACGCAGGCCGGGTTACGGAAGATAAAGTGCAGGGAATGGGCGTTGAATGTATTGATGATTGGGGCGGGGAAG
AAGGCGGCGTGTATTGCCGACGACCTGTATGGTTTGGGTTATAATATCGTCGGTTTTGTCTCCGATGGTACGGAGAAAAA
GGGCGGGGTCGCCGGAGATCGTGTGATCGGTAGGCTAGAATCTTTGCCGGTGATCGTAGAGGAGAAAAAGGTGGATGAGC
TGGTTGTCGCCTTGGAGACGGTGGATAATAACCGTTTGATGGATATCCTGTATTCGTTATATTGCTATAAACGGCCGATC
AAGATATTGGCGGATAAATCCAGTTCATTATCGCAAGTAAAGATCAAGGCGATAAAGGGCGTTCCGCTGGTGGACGTGAC
GGATAACAACTTCTTGCCCATCGAGCAGAATGTAAAGCTCTTTATGGATAAGACGTTATCTCTGATTTTCTTGGTGCTCT
TATCTCCCTTGTTCCTGTATATCGCTTGGCGGGTGAAGCGGGATTCCCCCGGCCCGGTGTTTTTCAGTCAGGAACGGATC
GGATACATGGGCGAGCCTTTCATGATCTATAAGTTCCGTACGATGTACACGAACGCCGAGGAGGAAGGCCCGTTGCTCTC
TTCCGAGGACGATTCCCGTATCACTCCCTTCGGACGGGTGATGCGGAAATACCGCTTGGACGAGTTACCGCAGTTCTGGA
ACGTGCTGAAAGGGGATATGTCGCTAGTTGGCCCCCGTCCGGAACGTAAATATTTCATCGAGCGGATCGTGAGGAAAGCC
CCCTTCTACTATCTGCTGCATAACGTACGTCCGGGAATCACCTCGTTGGGTATGGTGAAATATGGATATGCCGGCAGCGT
GGATGAGATGATCGAGCGCTTGGAATATGATATATTATACTATGAGAATATGTCCTTGATTCTGGACATAACGATTCTCA
TTTATACAGTTAAAACAGTGTTTACAGGTAAAGGTATTTAA

Upstream 100 bases:

>100_bases
ATTATATCGTGAGTTATCGTCAGGACGATATGAGCAAGGCCGCTCCGTCCGGCATTATCAAGTTAGGAGCCGGTGGGCTT
GTGCAGGTAATTAGATAAGG

Downstream 100 bases:

>100_bases
AATGGCAAACGAAGGTTTATTCTATAAATTCTTGTTGTGGAGGGAAAAGCGCATCAAGGAGAAGCATTTCATCCTTATTA
TCAGTTTTATCGTGGGACTT

Product: putative glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKKSVQAGKYIVSDFIASSVVWLLFNLIRYEEVAVYEGFDTVGAFLLHIPSLKGQLLIPFFWFVLYWFSGYYNKPFGKSR
LTELFSTLGSVSIGVVILFFALVLNDLPRSYHVYYDMFFSLWGLQFVLTYIPRLLITQAGLRKIKCREWALNVLMIGAGK
KAACIADDLYGLGYNIVGFVSDGTEKKGGVAGDRVIGRLESLPVIVEEKKVDELVVALETVDNNRLMDILYSLYCYKRPI
KILADKSSSLSQVKIKAIKGVPLVDVTDNNFLPIEQNVKLFMDKTLSLIFLVLLSPLFLYIAWRVKRDSPGPVFFSQERI
GYMGEPFMIYKFRTMYTNAEEEGPLLSSEDDSRITPFGRVMRKYRLDELPQFWNVLKGDMSLVGPRPERKYFIERIVRKA
PFYYLLHNVRPGITSLGMVKYGYAGSVDEMIERLEYDILYYENMSLILDITILIYTVKTVFTGKGI

Sequences:

>Translated_466_residues
MKKSVQAGKYIVSDFIASSVVWLLFNLIRYEEVAVYEGFDTVGAFLLHIPSLKGQLLIPFFWFVLYWFSGYYNKPFGKSR
LTELFSTLGSVSIGVVILFFALVLNDLPRSYHVYYDMFFSLWGLQFVLTYIPRLLITQAGLRKIKCREWALNVLMIGAGK
KAACIADDLYGLGYNIVGFVSDGTEKKGGVAGDRVIGRLESLPVIVEEKKVDELVVALETVDNNRLMDILYSLYCYKRPI
KILADKSSSLSQVKIKAIKGVPLVDVTDNNFLPIEQNVKLFMDKTLSLIFLVLLSPLFLYIAWRVKRDSPGPVFFSQERI
GYMGEPFMIYKFRTMYTNAEEEGPLLSSEDDSRITPFGRVMRKYRLDELPQFWNVLKGDMSLVGPRPERKYFIERIVRKA
PFYYLLHNVRPGITSLGMVKYGYAGSVDEMIERLEYDILYYENMSLILDITILIYTVKTVFTGKGI
>Mature_466_residues
MKKSVQAGKYIVSDFIASSVVWLLFNLIRYEEVAVYEGFDTVGAFLLHIPSLKGQLLIPFFWFVLYWFSGYYNKPFGKSR
LTELFSTLGSVSIGVVILFFALVLNDLPRSYHVYYDMFFSLWGLQFVLTYIPRLLITQAGLRKIKCREWALNVLMIGAGK
KAACIADDLYGLGYNIVGFVSDGTEKKGGVAGDRVIGRLESLPVIVEEKKVDELVVALETVDNNRLMDILYSLYCYKRPI
KILADKSSSLSQVKIKAIKGVPLVDVTDNNFLPIEQNVKLFMDKTLSLIFLVLLSPLFLYIAWRVKRDSPGPVFFSQERI
GYMGEPFMIYKFRTMYTNAEEEGPLLSSEDDSRITPFGRVMRKYRLDELPQFWNVLKGDMSLVGPRPERKYFIERIVRKA
PFYYLLHNVRPGITSLGMVKYGYAGSVDEMIERLEYDILYYENMSLILDITILIYTVKTVFTGKGI

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=381, Percent_Identity=30.1837270341207, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53446; Mature: 53446

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVQAGKYIVSDFIASSVVWLLFNLIRYEEVAVYEGFDTVGAFLLHIPSLKGQLLIPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCCCCHHHH
FWFVLYWFSGYYNKPFGKSRLTELFSTLGSVSIGVVILFFALVLNDLPRSYHVYYDMFFS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LWGLQFVLTYIPRLLITQAGLRKIKCREWALNVLMIGAGKKAACIADDLYGLGYNIVGFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHCCCEEEEEE
SDGTEKKGGVAGDRVIGRLESLPVIVEEKKVDELVVALETVDNNRLMDILYSLYCYKRPI
CCCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
KILADKSSSLSQVKIKAIKGVPLVDVTDNNFLPIEQNVKLFMDKTLSLIFLVLLSPLFLY
HHEECCCCCHHHEEHEEECCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAWRVKRDSPGPVFFSQERIGYMGEPFMIYKFRTMYTNAEEEGPLLSSEDDSRITPFGRV
HHHEEECCCCCCEEECCCCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCCCCCCHHHHH
MRKYRLDELPQFWNVLKGDMSLVGPRPERKYFIERIVRKAPFYYLLHNVRPGITSLGMVK
HHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCHHH
YGYAGSVDEMIERLEYDILYYENMSLILDITILIYTVKTVFTGKGI
CCCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKSVQAGKYIVSDFIASSVVWLLFNLIRYEEVAVYEGFDTVGAFLLHIPSLKGQLLIPF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHCCCCCCCCHHHH
FWFVLYWFSGYYNKPFGKSRLTELFSTLGSVSIGVVILFFALVLNDLPRSYHVYYDMFFS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LWGLQFVLTYIPRLLITQAGLRKIKCREWALNVLMIGAGKKAACIADDLYGLGYNIVGFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHCCCEEEEEE
SDGTEKKGGVAGDRVIGRLESLPVIVEEKKVDELVVALETVDNNRLMDILYSLYCYKRPI
CCCCCCCCCCCHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
KILADKSSSLSQVKIKAIKGVPLVDVTDNNFLPIEQNVKLFMDKTLSLIFLVLLSPLFLY
HHEECCCCCHHHEEHEEECCCEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
IAWRVKRDSPGPVFFSQERIGYMGEPFMIYKFRTMYTNAEEEGPLLSSEDDSRITPFGRV
HHHEEECCCCCCEEECCCCCCCCCCCEEEEEEEEHCCCCCCCCCCCCCCCCCCCCHHHHH
MRKYRLDELPQFWNVLKGDMSLVGPRPERKYFIERIVRKAPFYYLLHNVRPGITSLGMVK
HHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCHHH
YGYAGSVDEMIERLEYDILYYENMSLILDITILIYTVKTVFTGKGI
CCCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]