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The map label for this gene is ansA [H]

Identifier: 150008333

GI number: 150008333

Start: 2021023

End: 2022099

Strand: Reverse

Name: ansA [H]

Synonym: BDI_1705

Alternate gene names: 150008333

Gene position: 2022099-2021023 (Counterclockwise)

Preceding gene: 150008334

Following gene: 150008332

Centisome position: 42.03

GC content: 49.12

Gene sequence:

>1077_bases
TTGCATAATTATATGGACATGCATACTCAACAAGAAAAGGCTTCCATCCTGTTGATATATACGGGTGGGACGATAGGTAT
GATCGAGAATCCGGAGACTGGAGTGTTAGAGTCGTTTAATTTCCAGCATTTGAAGGACAATATGCCGGAGTTGAAGAAAC
TGGGGTATTCGGTGGCCACGATACAGTTCGATCCTCCGATGGACTCCTCGGAGATGGGGCCGGACTCGTGGATGAAGATC
GTGAAAATCATCGCCGATAATTATCAGAAATACGACGGCTTCGTGGTACTGCATGGCACGGATACAATGTCGTATACGGC
ATCGGCATTGAGTTTTATGCTTGAGAACCTAAGTAAGCCGGTGATCCTTACGGGATCGCAATTACCGATCGGGATGTTGC
GTACCGATGGGAAGGAGAACTTGATTACGGCGATCGAGATAGCGGCTGCCAAGGAGAACGGCTTACCGTTGGTCCCGGAG
GTCTGTATCTTTTTTGAGAATGATTTATTGAGGGGGAATCGTACGAGTAAGATAAACGCAGATAATTTTAATGCGTTCCG
TTCGTACAATTACCCGGCTTTGGCCCATGCGGGTATCTATATCAAGTATGATCTGCCGCAGGTATATCATCCGGTATCGC
GTAAGCCGCTGAAGCCGCATTATTTGTTGGACCGAAATATCGCTATACTAAAACTATTCCCCGGAATTTCTCCGCAAGTA
GTGGAAAGTATCCTGAATATCCCGGGTCTGAAAGGTGTGGTGATGGAAACATTCGGAAGCGGAAATGCTCCGTGCGAGGA
ATGGTTCTTGAATATGTTGAAGGAGGCTGTAGACCGTGGTATCGTTATCGTGAATGTTACTCAGTGTAGGGCCGGAAGTG
TGGAGATGCACCGTTATGAGACGGGGCACAAATTGTTGGAGGCAGGCGTGACGAGCGGCTTCGATAGTACGACGGAGAGC
GCCGTGACGAAGCTAATGTTCCTCTTCGGCCATGGGCTGACACCCGACGAGGTGAAGGAGCATATGAACTGCTCGTTGAT
CGGGGAGGTATCGATCCCTGAGACGTTCCGCCCCTAG

Upstream 100 bases:

>100_bases
CAGTTCGTATCGGCGTTGAGCGTGTTCCTCGCACGTATAATCGCCGTAAAATATCATATAAGTGTTCCGGTGTTGAAAGG
AGAAGAATAATTTATTAACT

Downstream 100 bases:

>100_bases
TGTATACTATGTAAAACAGAAAAAAAGCAGTGTTGCGGAATAGGAGAGTATGAAACTATCATTTCTGAGTTTAGCGAGTG
GGAGCAGTGGGAACTGCTAT

Product: L-asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MHNYMDMHTQQEKASILLIYTGGTIGMIENPETGVLESFNFQHLKDNMPELKKLGYSVATIQFDPPMDSSEMGPDSWMKI
VKIIADNYQKYDGFVVLHGTDTMSYTASALSFMLENLSKPVILTGSQLPIGMLRTDGKENLITAIEIAAAKENGLPLVPE
VCIFFENDLLRGNRTSKINADNFNAFRSYNYPALAHAGIYIKYDLPQVYHPVSRKPLKPHYLLDRNIAILKLFPGISPQV
VESILNIPGLKGVVMETFGSGNAPCEEWFLNMLKEAVDRGIVIVNVTQCRAGSVEMHRYETGHKLLEAGVTSGFDSTTES
AVTKLMFLFGHGLTPDEVKEHMNCSLIGEVSIPETFRP

Sequences:

>Translated_358_residues
MHNYMDMHTQQEKASILLIYTGGTIGMIENPETGVLESFNFQHLKDNMPELKKLGYSVATIQFDPPMDSSEMGPDSWMKI
VKIIADNYQKYDGFVVLHGTDTMSYTASALSFMLENLSKPVILTGSQLPIGMLRTDGKENLITAIEIAAAKENGLPLVPE
VCIFFENDLLRGNRTSKINADNFNAFRSYNYPALAHAGIYIKYDLPQVYHPVSRKPLKPHYLLDRNIAILKLFPGISPQV
VESILNIPGLKGVVMETFGSGNAPCEEWFLNMLKEAVDRGIVIVNVTQCRAGSVEMHRYETGHKLLEAGVTSGFDSTTES
AVTKLMFLFGHGLTPDEVKEHMNCSLIGEVSIPETFRP
>Mature_358_residues
MHNYMDMHTQQEKASILLIYTGGTIGMIENPETGVLESFNFQHLKDNMPELKKLGYSVATIQFDPPMDSSEMGPDSWMKI
VKIIADNYQKYDGFVVLHGTDTMSYTASALSFMLENLSKPVILTGSQLPIGMLRTDGKENLITAIEIAAAKENGLPLVPE
VCIFFENDLLRGNRTSKINADNFNAFRSYNYPALAHAGIYIKYDLPQVYHPVSRKPLKPHYLLDRNIAILKLFPGISPQV
VESILNIPGLKGVVMETFGSGNAPCEEWFLNMLKEAVDRGIVIVNVTQCRAGSVEMHRYETGHKLLEAGVTSGFDSTTES
AVTKLMFLFGHGLTPDEVKEHMNCSLIGEVSIPETFRP

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=367, Percent_Identity=40.8719346049046, Blast_Score=238, Evalue=5e-63,
Organism=Escherichia coli, GI1788065, Length=342, Percent_Identity=45.0292397660819, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1789327, Length=257, Percent_Identity=26.0700389105058, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=367, Percent_Identity=38.1471389645777, Blast_Score=238, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI17558280, Length=367, Percent_Identity=38.1471389645777, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI17558278, Length=367, Percent_Identity=38.1471389645777, Blast_Score=238, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI193207374, Length=367, Percent_Identity=38.1471389645777, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6320527, Length=317, Percent_Identity=26.4984227129338, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323189, Length=217, Percent_Identity=29.9539170506912, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323187, Length=217, Percent_Identity=29.9539170506912, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323186, Length=217, Percent_Identity=29.9539170506912, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323184, Length=217, Percent_Identity=29.9539170506912, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24645530, Length=344, Percent_Identity=44.4767441860465, Blast_Score=271, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24639609, Length=374, Percent_Identity=39.8395721925134, Blast_Score=258, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 39827; Mature: 39827

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNYMDMHTQQEKASILLIYTGGTIGMIENPETGVLESFNFQHLKDNMPELKKLGYSVAT
CCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHCCCEEEE
IQFDPPMDSSEMGPDSWMKIVKIIADNYQKYDGFVVLHGTDTMSYTASALSFMLENLSKP
EEECCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
VILTGSQLPIGMLRTDGKENLITAIEIAAAKENGLPLVPEVCIFFENDLLRGNRTSKINA
EEEECCCCCEEHEECCCCCCEEEEEEEEEECCCCCCCCCHHHEEECCCCCCCCCCCEECC
DNFNAFRSYNYPALAHAGIYIKYDLPQVYHPVSRKPLKPHYLLDRNIAILKLFPGISPQV
CCCHHHHCCCCCCEECCCEEEEECCHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCHHH
VESILNIPGLKGVVMETFGSGNAPCEEWFLNMLKEAVDRGIVIVNVTQCRAGSVEMHRYE
HHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEHH
TGHKLLEAGVTSGFDSTTESAVTKLMFLFGHGLTPDEVKEHMNCSLIGEVSIPETFRP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MHNYMDMHTQQEKASILLIYTGGTIGMIENPETGVLESFNFQHLKDNMPELKKLGYSVAT
CCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCCCCCCCCHHHHHCCCHHHHHCCCEEEE
IQFDPPMDSSEMGPDSWMKIVKIIADNYQKYDGFVVLHGTDTMSYTASALSFMLENLSKP
EEECCCCCCCCCCHHHHHHHHHHHHHCCHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
VILTGSQLPIGMLRTDGKENLITAIEIAAAKENGLPLVPEVCIFFENDLLRGNRTSKINA
EEEECCCCCEEHEECCCCCCEEEEEEEEEECCCCCCCCCHHHEEECCCCCCCCCCCEECC
DNFNAFRSYNYPALAHAGIYIKYDLPQVYHPVSRKPLKPHYLLDRNIAILKLFPGISPQV
CCCHHHHCCCCCCEECCCEEEEECCHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCHHH
VESILNIPGLKGVVMETFGSGNAPCEEWFLNMLKEAVDRGIVIVNVTQCRAGSVEMHRYE
HHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCEEEEEHH
TGHKLLEAGVTSGFDSTTESAVTKLMFLFGHGLTPDEVKEHMNCSLIGEVSIPETFRP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]