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The map label for this gene is topB [H]

Identifier: 150008318

GI number: 150008318

Start: 2000960

End: 2003056

Strand: Direct

Name: topB [H]

Synonym: BDI_1687

Alternate gene names: 150008318

Gene position: 2000960-2003056 (Clockwise)

Preceding gene: 150008316

Following gene: 150008319

Centisome position: 41.59

GC content: 49.12

Gene sequence:

>2097_bases
TTGATGAAAGTTTGTATCGCCGAAAAGCCGAGTGTGGCCCGGGAAATCGCGGAAGTATTAGGGGCAACCCAACGAATGAA
TGGGTATATCGAAGGAAACGGATATCAGGTAACTTGGACATTCGGGCATTTGTGTACCCTGAAAGAACCGCATGAATACG
CGGAGAACTGGAAACGCTGGAGTCTGGGGTCACTACCTATGATCCCTCCCCGGTTCGGTATCAAACTAATCGAGAATCCT
ACGTATGAGCAACAATTCAAGGTAATCGAGAGCCTGATGCAAAATGCCGAGATGGTCATTAACTGCGGTGATGCCGGCCA
AGAGGGAGAGCTGATCCAACGCTGGGTCATGCAAAAGGCAGGTTGTAAATGCCCGGTCTACCGCCTTTGGATCTCCTCGC
TTACGGAGGAGGCGATTCGGGAAGGTTTCCAAAAACTAAAAGAACAGACGGAATTCAATAAGTTATATGAGGCCGGGCTA
TCCCGTGCCATCGGTGACTGGTTGCTGGGGATGAACGCCACCCGCCTTTATACCTTGCGCTACGGGCAGAACCGGCAGGT
ATTATCGATCGGCCGTGTGCAAACCCCGACGCTCGCCTTGATCGTAAACCGGCAAGCCGAGATCGACAATTTCAAACCGG
AACCCTATTGGGAATTAAAGACCATTTACAGGAATACGACTTTCTCCGCCACGAAAGGTAAGTTCACGAAGAAAGAGGAA
GGTGAGGCTTTTCTGGAGAAGGTAAAGGAACAGGATTTTACCGTTACCAGTATCACCGAGAAGAAAGGGAAGGAATTCGC
CCCTCGCCTATTCGACTTGACCTCGTTGCAGGTGGAATGTAATAAGAAATTCGCGTTTACGGCGGACGATACCCTAAAGC
TGATCCAATCGCTCTACGAGAAGAAGGTGACAACCTACCCGCGTGTCGACACGACTTTCCTTAGTGATGATATTTATCCG
AAAGTTCCCAATACCTTGAAAGGGCTGGTGGATTATACGGAGCTTACCGCTCCTTTGATGAATCAGAAATTGCCGAAGAG
CAAGAAAGTGTTTGATAACTCGAAGGTTACCGATCACCATGCGATCATCCCTACCGGAGTCCCCGCCCGCAATCTTACGG
ATACGGAACGGAAGGTGTACGATTTGGTAGCCCGTCGTTTTATCGCCGCCTTTTATCCGGATTGCGAGATATCGACCACT
ACGGTCTTGGGTCAAGTGGATAAGGTGGAATTCAAGGTAACCGGAAAGCAAATCCTCAAACCGGGTTGGCGTGTCGTATT
CGGAGCCGAGCAGAAAGATCCGGAGGCTGAACCTACCGAGGAAGAAGGCGTATTACCCGATTTCGTGAAAGGAGAAAGCG
GCCCGCATAAACCGATCTTGAAAGAGACTTGGACCCAGCCTCCGAAACCTTATACAGAAGCTACTTTATTGCGGGCGATG
GAAACCGCCGGTAAATTAGTGGATAATGACGAGCTGCGGGATGCCTTAAAGGAAAACGGCATCGGCCGTCCTTCTACCCG
TGCGGCGATCATCGAGACTTTATTTAAGCGTAATTATATACGAAAGGAACGTAAGAATCTTTATCCCACCGCTACCGGCG
CGGAATTGATCGGCACGATCCATGAGGAACTGCTGAAAAGCGCAGAGTTGACGGGGCTTTGGGAAAAGAAACTACGCCAG
ATTGAACGAGGGACTTATGAGGCCCGTACTTTTCTGGATGAGTTGAAGCAGATGGTAAACGAGGTCGTCATAAACGTCCT
TTCCGACCAAACCCGGCGAACGATTACGATTGAGGATACGTCTAAAGCAGCTAAAGAAACTCCCAAAGACGAACCCAAGG
AGAAAAAGGAAAAGAAACCTCGTAAGCCTCGTGCCAAGAAAGAGAAAGCAGAGGCTACTCCCGAATTGGAGCTTCCATCA
ACGGATAAGCCCGTTTGCCCCATCTGTCACAAAGGCTCTATCCTCCGGGGCAAAACCGCTTATGGCTGCTCTGAATACAA
GAATGGATGTACGTTCCGCTTGGATTACGCTACGTATGGAAATAATTTAACGGATGAGGAGTTAGGTACGGTTATCAGTA
AATTAAAGTCTATTTAA

Upstream 100 bases:

>100_bases
ACTCCATCATACAGCTTTATAAACTCACGATAACAAACATCTTTGCTCATTCGGAAATAATCATGTATTTTTGTCTGTTC
ATTTAAGAAAGGATGATGTA

Downstream 100 bases:

>100_bases
AATATGAGAACAGAATGGTTTCCTCTGGTAAACGAGGAGGGTGAGACGATCGGAAAGGCTACTCGCAAGGAATGTCATAG
CGGAAGTAAGTTGCTACATC

Product: putative DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MMKVCIAEKPSVAREIAEVLGATQRMNGYIEGNGYQVTWTFGHLCTLKEPHEYAENWKRWSLGSLPMIPPRFGIKLIENP
TYEQQFKVIESLMQNAEMVINCGDAGQEGELIQRWVMQKAGCKCPVYRLWISSLTEEAIREGFQKLKEQTEFNKLYEAGL
SRAIGDWLLGMNATRLYTLRYGQNRQVLSIGRVQTPTLALIVNRQAEIDNFKPEPYWELKTIYRNTTFSATKGKFTKKEE
GEAFLEKVKEQDFTVTSITEKKGKEFAPRLFDLTSLQVECNKKFAFTADDTLKLIQSLYEKKVTTYPRVDTTFLSDDIYP
KVPNTLKGLVDYTELTAPLMNQKLPKSKKVFDNSKVTDHHAIIPTGVPARNLTDTERKVYDLVARRFIAAFYPDCEISTT
TVLGQVDKVEFKVTGKQILKPGWRVVFGAEQKDPEAEPTEEEGVLPDFVKGESGPHKPILKETWTQPPKPYTEATLLRAM
ETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLYPTATGAELIGTIHEELLKSAELTGLWEKKLRQ
IERGTYEARTFLDELKQMVNEVVINVLSDQTRRTITIEDTSKAAKETPKDEPKEKKEKKPRKPRAKKEKAEATPELELPS
TDKPVCPICHKGSILRGKTAYGCSEYKNGCTFRLDYATYGNNLTDEELGTVISKLKSI

Sequences:

>Translated_698_residues
MMKVCIAEKPSVAREIAEVLGATQRMNGYIEGNGYQVTWTFGHLCTLKEPHEYAENWKRWSLGSLPMIPPRFGIKLIENP
TYEQQFKVIESLMQNAEMVINCGDAGQEGELIQRWVMQKAGCKCPVYRLWISSLTEEAIREGFQKLKEQTEFNKLYEAGL
SRAIGDWLLGMNATRLYTLRYGQNRQVLSIGRVQTPTLALIVNRQAEIDNFKPEPYWELKTIYRNTTFSATKGKFTKKEE
GEAFLEKVKEQDFTVTSITEKKGKEFAPRLFDLTSLQVECNKKFAFTADDTLKLIQSLYEKKVTTYPRVDTTFLSDDIYP
KVPNTLKGLVDYTELTAPLMNQKLPKSKKVFDNSKVTDHHAIIPTGVPARNLTDTERKVYDLVARRFIAAFYPDCEISTT
TVLGQVDKVEFKVTGKQILKPGWRVVFGAEQKDPEAEPTEEEGVLPDFVKGESGPHKPILKETWTQPPKPYTEATLLRAM
ETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLYPTATGAELIGTIHEELLKSAELTGLWEKKLRQ
IERGTYEARTFLDELKQMVNEVVINVLSDQTRRTITIEDTSKAAKETPKDEPKEKKEKKPRKPRAKKEKAEATPELELPS
TDKPVCPICHKGSILRGKTAYGCSEYKNGCTFRLDYATYGNNLTDEELGTVISKLKSI
>Mature_698_residues
MMKVCIAEKPSVAREIAEVLGATQRMNGYIEGNGYQVTWTFGHLCTLKEPHEYAENWKRWSLGSLPMIPPRFGIKLIENP
TYEQQFKVIESLMQNAEMVINCGDAGQEGELIQRWVMQKAGCKCPVYRLWISSLTEEAIREGFQKLKEQTEFNKLYEAGL
SRAIGDWLLGMNATRLYTLRYGQNRQVLSIGRVQTPTLALIVNRQAEIDNFKPEPYWELKTIYRNTTFSATKGKFTKKEE
GEAFLEKVKEQDFTVTSITEKKGKEFAPRLFDLTSLQVECNKKFAFTADDTLKLIQSLYEKKVTTYPRVDTTFLSDDIYP
KVPNTLKGLVDYTELTAPLMNQKLPKSKKVFDNSKVTDHHAIIPTGVPARNLTDTERKVYDLVARRFIAAFYPDCEISTT
TVLGQVDKVEFKVTGKQILKPGWRVVFGAEQKDPEAEPTEEEGVLPDFVKGESGPHKPILKETWTQPPKPYTEATLLRAM
ETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLYPTATGAELIGTIHEELLKSAELTGLWEKKLRQ
IERGTYEARTFLDELKQMVNEVVINVLSDQTRRTITIEDTSKAAKETPKDEPKEKKEKKPRKPRAKKEKAEATPELELPS
TDKPVCPICHKGSILRGKTAYGCSEYKNGCTFRLDYATYGNNLTDEELGTVISKLKSI

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=615, Percent_Identity=24.390243902439, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI10835218, Length=725, Percent_Identity=23.0344827586207, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788061, Length=656, Percent_Identity=32.469512195122, Blast_Score=321, Evalue=1e-88,
Organism=Escherichia coli, GI1787529, Length=522, Percent_Identity=26.4367816091954, Blast_Score=132, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=573, Percent_Identity=24.6073298429319, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=616, Percent_Identity=25.487012987013, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=622, Percent_Identity=25.7234726688103, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24640096, Length=573, Percent_Identity=24.78184991274, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585251, Length=645, Percent_Identity=25.4263565891473, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79449; Mature: 79449

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKVCIAEKPSVAREIAEVLGATQRMNGYIEGNGYQVTWTFGHLCTLKEPHEYAENWKRW
CCEEEECCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEEEECCEEECCCCHHHHHHHCCC
SLGSLPMIPPRFGIKLIENPTYEQQFKVIESLMQNAEMVINCGDAGQEGELIQRWVMQKA
CCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHC
GCKCPVYRLWISSLTEEAIREGFQKLKEQTEFNKLYEAGLSRAIGDWLLGMNATRLYTLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YGQNRQVLSIGRVQTPTLALIVNRQAEIDNFKPEPYWELKTIYRNTTFSATKGKFTKKEE
ECCCCCEEEECCCCCCEEEEEEECCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHH
GEAFLEKVKEQDFTVTSITEKKGKEFAPRLFDLTSLQVECNKKFAFTADDTLKLIQSLYE
HHHHHHHHHHCCCEEEEEHHHCCHHHCCHHHHHHEEEEEECCEEEECCHHHHHHHHHHHH
KKVTTYPRVDTTFLSDDIYPKVPNTLKGLVDYTELTAPLMNQKLPKSKKVFDNSKVTDHH
HHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCC
AIIPTGVPARNLTDTERKVYDLVARRFIAAFYPDCEISTTTVLGQVDKVEFKVTGKQILK
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCHHHCC
PGWRVVFGAEQKDPEAEPTEEEGVLPDFVKGESGPHKPILKETWTQPPKPYTEATLLRAM
CCCEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
ETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLYPTATGAELIGTI
HHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
HEELLKSAELTGLWEKKLRQIERGTYEARTFLDELKQMVNEVVINVLSDQTRRTITIEDT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
SKAAKETPKDEPKEKKEKKPRKPRAKKEKAEATPELELPSTDKPVCPICHKGSILRGKTA
HHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCC
YGCSEYKNGCTFRLDYATYGNNLTDEELGTVISKLKSI
CCHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKVCIAEKPSVAREIAEVLGATQRMNGYIEGNGYQVTWTFGHLCTLKEPHEYAENWKRW
CCEEEECCCCHHHHHHHHHHHHHHHHCCEEECCCEEEEEEECCEEECCCCHHHHHHHCCC
SLGSLPMIPPRFGIKLIENPTYEQQFKVIESLMQNAEMVINCGDAGQEGELIQRWVMQKA
CCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHC
GCKCPVYRLWISSLTEEAIREGFQKLKEQTEFNKLYEAGLSRAIGDWLLGMNATRLYTLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
YGQNRQVLSIGRVQTPTLALIVNRQAEIDNFKPEPYWELKTIYRNTTFSATKGKFTKKEE
ECCCCCEEEECCCCCCEEEEEEECCHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHH
GEAFLEKVKEQDFTVTSITEKKGKEFAPRLFDLTSLQVECNKKFAFTADDTLKLIQSLYE
HHHHHHHHHHCCCEEEEEHHHCCHHHCCHHHHHHEEEEEECCEEEECCHHHHHHHHHHHH
KKVTTYPRVDTTFLSDDIYPKVPNTLKGLVDYTELTAPLMNQKLPKSKKVFDNSKVTDHH
HHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCC
AIIPTGVPARNLTDTERKVYDLVARRFIAAFYPDCEISTTTVLGQVDKVEFKVTGKQILK
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEEEEECCHHHCC
PGWRVVFGAEQKDPEAEPTEEEGVLPDFVKGESGPHKPILKETWTQPPKPYTEATLLRAM
CCCEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHH
ETAGKLVDNDELRDALKENGIGRPSTRAAIIETLFKRNYIRKERKNLYPTATGAELIGTI
HHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
HEELLKSAELTGLWEKKLRQIERGTYEARTFLDELKQMVNEVVINVLSDQTRRTITIEDT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
SKAAKETPKDEPKEKKEKKPRKPRAKKEKAEATPELELPSTDKPVCPICHKGSILRGKTA
HHHHHCCCCCCCHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCC
YGCSEYKNGCTFRLDYATYGNNLTDEELGTVISKLKSI
CCHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA