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The map label for this gene is icaA [H]

Identifier: 150007936

GI number: 150007936

Start: 1506702

End: 1507889

Strand: Direct

Name: icaA [H]

Synonym: BDI_1297

Alternate gene names: 150007936

Gene position: 1506702-1507889 (Clockwise)

Preceding gene: 150007935

Following gene: 150007937

Centisome position: 31.32

GC content: 54.29

Gene sequence:

>1188_bases
ATGGAAAAGAGCTGTCAAGTCATTGAGATCCTCTTCTGGATCGGGATCGGGATCGTTTTCTATACCTATCTGGGGTATGG
GATCGTATTATACCTTATGGTCAAGATCAAGGAGCTGTTCGTAAAACCCCGGTCCCCCCGCCTTCCAGAGCCGCTGCCGG
AGGCCACGTTGCTGATAGCCGCCTACAACGAGGAGGCGATCGTGGCGAGCAAGATGGTGAATTGCAGACAACTGGATTAT
CCGGCGGATAAGCTGAGACTGGTCTGGATCACCGATGGAAGCAATGACAACACGAATGAGAGATTAAAAGAGTACCCGGA
GGTCACCGTGCTGTACCAACCACGGCGACAAGGCAAGACAGCCGCCTTGAACCGGGCCATCCCCTACGTGAAGACCCCCT
ACGTGATCTTCACCGACGCCAACACGATGCTGAACAAGGGAGCCATCAAGGAGATCATACGCCAGTTCAGCGACCCGCGG
GTAGGCTGCGTGGCCGGAGAGAAACGGGTAGAGATACAAGCCGAGCAAGGAGCCACCGCCGGAGAAGGCATATACTGGAA
ATACGAGTCCGCCCTCAAGCGACTGGACTACCGGTTATACTCCACCGTCGGAGCGGCAGGCGAGCTCTTCGCCATCCGTA
CCTCCCTCTTCGAGCAGATGCCCCCGGATACCCTGCTGGACGATTTCATCCTCTCCCTGCGCATAGCGATGAGAGGCTAC
AAGATCGCCTACAGCAAAGAGGCGTACGCCCTCGAGAGCGCCTCCCTGAACATGCGGGAGGAGGAGAAACGTAAAGTACG
CATATCGGCCGGAGGATTGCAATCGGTATGGCGGCTACGGGGATTATTGAATATCTTCCGGTACGGGATACTGAGCTTCC
AATACATCAGCCACCGGGTACTGCGCTGGACGCTGACGCCGGTCGTGCTCTTCGCCCTCCTGCCCCTCAACCTTCTCCTA
GCCTGTACCGGGCATACCCTTTACACGGTCATCCTTGCCCTGCAATTAGCGTTTTACCTGCTGGGATACTTGGGGTATAA
AATGGAAAAACGGAACATCCGGAACAAGCTATTATTCATTCCTTATTACTTTTTATTCATGAATATCAATGTCATACGAG
GGTATAGCTATCTCGCTAAGCATAAAGGCACAGGCGCTTGGGAAAAAGCCAAACGAGGCGCGGGATAA

Upstream 100 bases:

>100_bases
CGCCTCTCGATCTATCTCGTAAAAGGGAAATTCCGGCTGATCGGGGCGCTAGGCAAGGGATTAATAAACGGATTAATCGC
AATCAACAAGGATATGAATT

Downstream 100 bases:

>100_bases
AAAAATAAAAAAATAGATGGATAAATAGTCGTTATATGTCGACTTTAATTAATACTTTTGCGCTATTGATACTATTTGAA
TATCAAGAAAACTAGAACAC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MEKSCQVIEILFWIGIGIVFYTYLGYGIVLYLMVKIKELFVKPRSPRLPEPLPEATLLIAAYNEEAIVASKMVNCRQLDY
PADKLRLVWITDGSNDNTNERLKEYPEVTVLYQPRRQGKTAALNRAIPYVKTPYVIFTDANTMLNKGAIKEIIRQFSDPR
VGCVAGEKRVEIQAEQGATAGEGIYWKYESALKRLDYRLYSTVGAAGELFAIRTSLFEQMPPDTLLDDFILSLRIAMRGY
KIAYSKEAYALESASLNMREEEKRKVRISAGGLQSVWRLRGLLNIFRYGILSFQYISHRVLRWTLTPVVLFALLPLNLLL
ACTGHTLYTVILALQLAFYLLGYLGYKMEKRNIRNKLLFIPYYFLFMNINVIRGYSYLAKHKGTGAWEKAKRGAG

Sequences:

>Translated_395_residues
MEKSCQVIEILFWIGIGIVFYTYLGYGIVLYLMVKIKELFVKPRSPRLPEPLPEATLLIAAYNEEAIVASKMVNCRQLDY
PADKLRLVWITDGSNDNTNERLKEYPEVTVLYQPRRQGKTAALNRAIPYVKTPYVIFTDANTMLNKGAIKEIIRQFSDPR
VGCVAGEKRVEIQAEQGATAGEGIYWKYESALKRLDYRLYSTVGAAGELFAIRTSLFEQMPPDTLLDDFILSLRIAMRGY
KIAYSKEAYALESASLNMREEEKRKVRISAGGLQSVWRLRGLLNIFRYGILSFQYISHRVLRWTLTPVVLFALLPLNLLL
ACTGHTLYTVILALQLAFYLLGYLGYKMEKRNIRNKLLFIPYYFLFMNINVIRGYSYLAKHKGTGAWEKAKRGAG
>Mature_395_residues
MEKSCQVIEILFWIGIGIVFYTYLGYGIVLYLMVKIKELFVKPRSPRLPEPLPEATLLIAAYNEEAIVASKMVNCRQLDY
PADKLRLVWITDGSNDNTNERLKEYPEVTVLYQPRRQGKTAALNRAIPYVKTPYVIFTDANTMLNKGAIKEIIRQFSDPR
VGCVAGEKRVEIQAEQGATAGEGIYWKYESALKRLDYRLYSTVGAAGELFAIRTSLFEQMPPDTLLDDFILSLRIAMRGY
KIAYSKEAYALESASLNMREEEKRKVRISAGGLQSVWRLRGLLNIFRYGILSFQYISHRVLRWTLTPVVLFALLPLNLLL
ACTGHTLYTVILALQLAFYLLGYLGYKMEKRNIRNKLLFIPYYFLFMNINVIRGYSYLAKHKGTGAWEKAKRGAG

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=344, Percent_Identity=22.6744186046512, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 45232; Mature: 45232

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSCQVIEILFWIGIGIVFYTYLGYGIVLYLMVKIKELFVKPRSPRLPEPLPEATLLIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
AYNEEAIVASKMVNCRQLDYPADKLRLVWITDGSNDNTNERLKEYPEVTVLYQPRRQGKT
ECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCH
AALNRAIPYVKTPYVIFTDANTMLNKGAIKEIIRQFSDPRVGCVAGEKRVEIQAEQGATA
HHHHHCCCCEECCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCC
GEGIYWKYESALKRLDYRLYSTVGAAGELFAIRTSLFEQMPPDTLLDDFILSLRIAMRGY
CCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCE
KIAYSKEAYALESASLNMREEEKRKVRISAGGLQSVWRLRGLLNIFRYGILSFQYISHRV
EEEECCCHHEECCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRWTLTPVVLFALLPLNLLLACTGHTLYTVILALQLAFYLLGYLGYKMEKRNIRNKLLFI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
PYYFLFMNINVIRGYSYLAKHKGTGAWEKAKRGAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure
MEKSCQVIEILFWIGIGIVFYTYLGYGIVLYLMVKIKELFVKPRSPRLPEPLPEATLLIA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
AYNEEAIVASKMVNCRQLDYPADKLRLVWITDGSNDNTNERLKEYPEVTVLYQPRRQGKT
ECCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCEEEEECCCCCCCH
AALNRAIPYVKTPYVIFTDANTMLNKGAIKEIIRQFSDPRVGCVAGEKRVEIQAEQGATA
HHHHHCCCCEECCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCC
GEGIYWKYESALKRLDYRLYSTVGAAGELFAIRTSLFEQMPPDTLLDDFILSLRIAMRGY
CCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCE
KIAYSKEAYALESASLNMREEEKRKVRISAGGLQSVWRLRGLLNIFRYGILSFQYISHRV
EEEECCCHHEECCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRWTLTPVVLFALLPLNLLLACTGHTLYTVILALQLAFYLLGYLGYKMEKRNIRNKLLFI
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
PYYFLFMNINVIRGYSYLAKHKGTGAWEKAKRGAG
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]