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The map label for this gene is dnaG [H]

Identifier: 150007929

GI number: 150007929

Start: 1500558

End: 1502300

Strand: Direct

Name: dnaG [H]

Synonym: BDI_1290

Alternate gene names: 150007929

Gene position: 1500558-1502300 (Clockwise)

Preceding gene: 150007928

Following gene: 150007931

Centisome position: 31.19

GC content: 67.36

Gene sequence:

>1743_bases
ATGATCACGCATGACACTTTACTGCGGGTACGGGAAACCGCCCGCATCGACGAGGTAGCGGCCGGGTACCTCACACTACG
GCGTTCCGGCAAGGGTCTCGTGGCGCTCTGCCCTTTCCACGACGAGCGTCACCCCTCGTTCCGGATCAGCCCGGCGCTCA
ATATCGGAAAATGCTTCAGTTGCGGCGAGGCAGCGGACCCCATACGCCTCGTGCGCCACATGGAGGGATGCGGCTTCGAG
GAGGCCGTGCGGCTGCTGGCCCGCAAGTATGGCATCGAGGTGGAGGAGGAACGGGGCACGGAGGAGGCCGGACGGCACGA
GGCACGACAGGCGATCTTACGCGGCAACGAGGACTTCGCCCGCTCCCTCCTTCCCTACGATCCCGCCGAGGGGATAACTG
GTAAGGACGAGAACGGCGAAGAAGACCGCAGGGCGCTCCGTGAAGCCTTTTCCCACTTCGGGGTAGGTATCTGCCCGCCC
GACGCCCCCGAGGGCTTCAGGCGCTTCCGGAGGCGGCTTGTCTTCCCGGTAAGGACCACCGGGGGACAGATAGCGGGCTT
CGCCGGGCGGTACCGGGGAACGGAGGGGGCGGGAACAGCCAAATACGTCAACTCCGACAACAGCGAGGCGTACAACAAGG
GACGTATCCTCTACGGCCTGTACGAGGCGGTGAGGGCCGTCCGCACCGAGGGCGACGTGCTGCTGTGCGAGGGTTACAAG
GACGTGATCGCCTTCCACGCCGCCGGTATCCGTCACGCCGCCGGGCTTTGCGGCACGGCGCTCACCGAGGATCACGTGCG
GATGATACGGAGGCTGACGGGGCACGTCACGCTCGCCCTCGACCCGGACCCGGCGGGACAGGCCGCCACCCTCCGCTCCG
CCCGTCTCTTGCTGGCACAGGGATGCGAGGTGGGCCTCCTTCCCCTGCCCGGTGGCATGGACCCGGACGAGATGTTCCGC
CGGCAAGGGGCCGAGGCGCTGCGCAGGCTCGTGCGGACAGAGGCGGTGGATTACCTGAGCCACCGCATCCGGAAGGCGGC
GGGGCGGAAAGGCGGACCCGACGCCGGGGGGATACGGGAGGTGCTCGGGGAGATCCGTCTCGTAGGCTCTCCGCTGGCCG
TGTACCAGCGGGTGGAGGAACTCTCCAAGGCTACGGGTATCCCCCTCGACGTATTGCGTGAGGAGTTGTCCGCCTCTCCC
GGCGAGCCTGTCCGGACAGGGCCGGCGGAAGTAGTGACGGGCCTCCCTCCCGCACGCCTCCGGGAAAGGGCCTTGCTGCG
TTTCTGCCTCGCCAACCACGACCGGATGTTCTACGCCGGGGATGGTAGCGGTATCTCCCTCCCGGCATGGGTCGCCTCCG
AGTTGTCCGCCGGCGGGATGCCCCTGACGGAACCCGCCCATCTCGACCTGCTGGCTCTCCTCTCGGGAAGCGGCCATCCG
GACGGGATCACGGACGAGTCTCTCTCCGCCCTCATCCTCTCCCTCCGTTCCTCCTACCCGGAGGAGGGACCGCCGGAAAG
GCTCCCCGACGAACTTTTGCCCGACGAGGCCGAACGCCAGCTCTTCCTCTACGCCGAGCCTTTCATACACGACCGGCTAC
AACGCACGGCCGCACACCTCCGCTCCGCTACCTCGACCGATGAGCGGGCGACATGCCTGAAGGAACTGAGGGATTGTTTC
GCCTTGAGCGACCGGATCGGGAGGGCGCTGGGAAGCCAGAGCGTGAGGCGGGTGGAGGGCTGA

Upstream 100 bases:

>100_bases
TCTGCCACAACAAGGGGGTAACCCGGTTCACCGATGACCGCACGCCGGTGCCGAACGACCGCAGACCGGCGAAAGCAGAA
CTGGATAAAGACCTGTTCGG

Downstream 100 bases:

>100_bases
CCCGCCAACCATCAGCCGCCCACAAAAAAACACTACTGTGCTTGGCACCGAACACTATTGTGTCGGGAGGCCAACACAGT
AGTATCCGAAGCCAAACACA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MITHDTLLRVRETARIDEVAAGYLTLRRSGKGLVALCPFHDERHPSFRISPALNIGKCFSCGEAADPIRLVRHMEGCGFE
EAVRLLARKYGIEVEEERGTEEAGRHEARQAILRGNEDFARSLLPYDPAEGITGKDENGEEDRRALREAFSHFGVGICPP
DAPEGFRRFRRRLVFPVRTTGGQIAGFAGRYRGTEGAGTAKYVNSDNSEAYNKGRILYGLYEAVRAVRTEGDVLLCEGYK
DVIAFHAAGIRHAAGLCGTALTEDHVRMIRRLTGHVTLALDPDPAGQAATLRSARLLLAQGCEVGLLPLPGGMDPDEMFR
RQGAEALRRLVRTEAVDYLSHRIRKAAGRKGGPDAGGIREVLGEIRLVGSPLAVYQRVEELSKATGIPLDVLREELSASP
GEPVRTGPAEVVTGLPPARLRERALLRFCLANHDRMFYAGDGSGISLPAWVASELSAGGMPLTEPAHLDLLALLSGSGHP
DGITDESLSALILSLRSSYPEEGPPERLPDELLPDEAERQLFLYAEPFIHDRLQRTAAHLRSATSTDERATCLKELRDCF
ALSDRIGRALGSQSVRRVEG

Sequences:

>Translated_580_residues
MITHDTLLRVRETARIDEVAAGYLTLRRSGKGLVALCPFHDERHPSFRISPALNIGKCFSCGEAADPIRLVRHMEGCGFE
EAVRLLARKYGIEVEEERGTEEAGRHEARQAILRGNEDFARSLLPYDPAEGITGKDENGEEDRRALREAFSHFGVGICPP
DAPEGFRRFRRRLVFPVRTTGGQIAGFAGRYRGTEGAGTAKYVNSDNSEAYNKGRILYGLYEAVRAVRTEGDVLLCEGYK
DVIAFHAAGIRHAAGLCGTALTEDHVRMIRRLTGHVTLALDPDPAGQAATLRSARLLLAQGCEVGLLPLPGGMDPDEMFR
RQGAEALRRLVRTEAVDYLSHRIRKAAGRKGGPDAGGIREVLGEIRLVGSPLAVYQRVEELSKATGIPLDVLREELSASP
GEPVRTGPAEVVTGLPPARLRERALLRFCLANHDRMFYAGDGSGISLPAWVASELSAGGMPLTEPAHLDLLALLSGSGHP
DGITDESLSALILSLRSSYPEEGPPERLPDELLPDEAERQLFLYAEPFIHDRLQRTAAHLRSATSTDERATCLKELRDCF
ALSDRIGRALGSQSVRRVEG
>Mature_580_residues
MITHDTLLRVRETARIDEVAAGYLTLRRSGKGLVALCPFHDERHPSFRISPALNIGKCFSCGEAADPIRLVRHMEGCGFE
EAVRLLARKYGIEVEEERGTEEAGRHEARQAILRGNEDFARSLLPYDPAEGITGKDENGEEDRRALREAFSHFGVGICPP
DAPEGFRRFRRRLVFPVRTTGGQIAGFAGRYRGTEGAGTAKYVNSDNSEAYNKGRILYGLYEAVRAVRTEGDVLLCEGYK
DVIAFHAAGIRHAAGLCGTALTEDHVRMIRRLTGHVTLALDPDPAGQAATLRSARLLLAQGCEVGLLPLPGGMDPDEMFR
RQGAEALRRLVRTEAVDYLSHRIRKAAGRKGGPDAGGIREVLGEIRLVGSPLAVYQRVEELSKATGIPLDVLREELSASP
GEPVRTGPAEVVTGLPPARLRERALLRFCLANHDRMFYAGDGSGISLPAWVASELSAGGMPLTEPAHLDLLALLSGSGHP
DGITDESLSALILSLRSSYPEEGPPERLPDELLPDEAERQLFLYAEPFIHDRLQRTAAHLRSATSTDERATCLKELRDCF
ALSDRIGRALGSQSVRRVEG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=340, Percent_Identity=30.8823529411765, Blast_Score=162, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 63327; Mature: 63327

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITHDTLLRVRETARIDEVAAGYLTLRRSGKGLVALCPFHDERHPSFRISPALNIGKCFS
CCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCEEECCCCCCHHHCC
CGEAADPIRLVRHMEGCGFEEAVRLLARKYGIEVEEERGTEEAGRHEARQAILRGNEDFA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCHHHH
RSLLPYDPAEGITGKDENGEEDRRALREAFSHFGVGICPPDAPEGFRRFRRRLVFPVRTT
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEECC
GGQIAGFAGRYRGTEGAGTAKYVNSDNSEAYNKGRILYGLYEAVRAVRTEGDVLLCEGYK
CCCEECCHHCCCCCCCCCCCCEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEECCHH
DVIAFHAAGIRHAAGLCGTALTEDHVRMIRRLTGHVTLALDPDPAGQAATLRSARLLLAQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHC
GCEVGLLPLPGGMDPDEMFRRQGAEALRRLVRTEAVDYLSHRIRKAAGRKGGPDAGGIRE
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VLGEIRLVGSPLAVYQRVEELSKATGIPLDVLREELSASPGEPVRTGPAEVVTGLPPARL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHH
RERALLRFCLANHDRMFYAGDGSGISLPAWVASELSAGGMPLTEPAHLDLLALLSGSGHP
HHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
DGITDESLSALILSLRSSYPEEGPPERLPDELLPDEAERQLFLYAEPFIHDRLQRTAAHL
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHCEEEEEECHHHHHHHHHHHHHH
RSATSTDERATCLKELRDCFALSDRIGRALGSQSVRRVEG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITHDTLLRVRETARIDEVAAGYLTLRRSGKGLVALCPFHDERHPSFRISPALNIGKCFS
CCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCEEECCCCCCHHHCC
CGEAADPIRLVRHMEGCGFEEAVRLLARKYGIEVEEERGTEEAGRHEARQAILRGNEDFA
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHCCCHHHH
RSLLPYDPAEGITGKDENGEEDRRALREAFSHFGVGICPPDAPEGFRRFRRRLVFPVRTT
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEECC
GGQIAGFAGRYRGTEGAGTAKYVNSDNSEAYNKGRILYGLYEAVRAVRTEGDVLLCEGYK
CCCEECCHHCCCCCCCCCCCCEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCEEEECCHH
DVIAFHAAGIRHAAGLCGTALTEDHVRMIRRLTGHVTLALDPDPAGQAATLRSARLLLAQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHC
GCEVGLLPLPGGMDPDEMFRRQGAEALRRLVRTEAVDYLSHRIRKAAGRKGGPDAGGIRE
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VLGEIRLVGSPLAVYQRVEELSKATGIPLDVLREELSASPGEPVRTGPAEVVTGLPPARL
HHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHH
RERALLRFCLANHDRMFYAGDGSGISLPAWVASELSAGGMPLTEPAHLDLLALLSGSGHP
HHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
DGITDESLSALILSLRSSYPEEGPPERLPDELLPDEAERQLFLYAEPFIHDRLQRTAAHL
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCHHHCEEEEEECHHHHHHHHHHHHHH
RSATSTDERATCLKELRDCFALSDRIGRALGSQSVRRVEG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]