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The map label for this gene is lacA [C]

Identifier: 150007921

GI number: 150007921

Start: 1492178

End: 1492804

Strand: Direct

Name: lacA [C]

Synonym: BDI_1282

Alternate gene names: 150007921

Gene position: 1492178-1492804 (Clockwise)

Preceding gene: 150007920

Following gene: 150007923

Centisome position: 31.01

GC content: 41.47

Gene sequence:

>627_bases
ATGAATATCAGCGATCTTAAACAAAAGATAAAGAAAAATGACAGATTAAAGAGAACGGTTCATTACTTAATAATGAATCA
AAGAGGCTCTTGCCCTAGATTATGGGTTAAATGGTTTGTCAATCCATTTATGTTAACCTATGGAAAACGGAGCAAGATCA
GAAGATCTGCTATAATGAATGTCAGTCCCATTAACTCATTCATACTTGGACAGGAAAGTATAATTGAGCATTACGTCACG
ATAGACAACGGGGTAGGCCACGTACACATCGGTGATCATTCACGCATAGGCATACATAATACGATCATCGGTCCCGTTTT
TATTGGGAATCAAGTTATCTTGGCTCAGAACATCACTATATCCGGGCTCAACCATACATACCATGATATTTCTAAACCAA
TCGTAAAACAAGGCATAACTACCTCCCCTGTGATCATAGAAGATGAGACTTGGATAGGAGCGAACACGGTCATTACCTCA
GGCGTACATATCGGCAAGCACTGCGTGATCGGCGCGGGCAGTGTCGTCACAAAAGATATACCAGATTACTCTGTGGCCGT
AGGCAATCCTGCTAAAGTTGTCAAACATTATGACCCATGCGGACAGCAATGGAAACGAACCCGATAA

Upstream 100 bases:

>100_bases
AAATAAAATATGGATTACAAAGATCCCGATTATTCTCATGGAATCATACGGCTAGGAAACTATTGCAAGTTTATATGGAA
GTCATTCAATCCTTAAAGAA

Downstream 100 bases:

>100_bases
AAGGGATCAATGCGATTCCTCAAAATGATAACATTTCGCATAAAGACCGGCTATCTTATCGGGATGAAGATACTTGTCGG
CATACCTCCGGTTCTTAGCT

Product: putative acetyl transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNISDLKQKIKKNDRLKRTVHYLIMNQRGSCPRLWVKWFVNPFMLTYGKRSKIRRSAIMNVSPINSFILGQESIIEHYVT
IDNGVGHVHIGDHSRIGIHNTIIGPVFIGNQVILAQNITISGLNHTYHDISKPIVKQGITTSPVIIEDETWIGANTVITS
GVHIGKHCVIGAGSVVTKDIPDYSVAVGNPAKVVKHYDPCGQQWKRTR

Sequences:

>Translated_208_residues
MNISDLKQKIKKNDRLKRTVHYLIMNQRGSCPRLWVKWFVNPFMLTYGKRSKIRRSAIMNVSPINSFILGQESIIEHYVT
IDNGVGHVHIGDHSRIGIHNTIIGPVFIGNQVILAQNITISGLNHTYHDISKPIVKQGITTSPVIIEDETWIGANTVITS
GVHIGKHCVIGAGSVVTKDIPDYSVAVGNPAKVVKHYDPCGQQWKRTR
>Mature_208_residues
MNISDLKQKIKKNDRLKRTVHYLIMNQRGSCPRLWVKWFVNPFMLTYGKRSKIRRSAIMNVSPINSFILGQESIIEHYVT
IDNGVGHVHIGDHSRIGIHNTIIGPVFIGNQVILAQNITISGLNHTYHDISKPIVKQGITTSPVIIEDETWIGANTVITS
GVHIGKHCVIGAGSVVTKDIPDYSVAVGNPAKVVKHYDPCGQQWKRTR

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=131, Percent_Identity=36.6412213740458, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786664, Length=93, Percent_Identity=36.5591397849462, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1788345, Length=133, Percent_Identity=30.0751879699248, Blast_Score=61, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=170, Percent_Identity=35.8823529411765, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 23249; Mature: 23249

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISDLKQKIKKNDRLKRTVHYLIMNQRGSCPRLWVKWFVNPFMLTYGKRSKIRRSAIMN
CCHHHHHHHHHHCHHHHHHHHHHEECCCCCCHHHHHHHHHCHHHEECCCHHHHHHHHHCC
VSPINSFILGQESIIEHYVTIDNGVGHVHIGDHSRIGIHNTIIGPVFIGNQVILAQNITI
CCCCHHHHCCHHHHHHHHEEEECCCCEEEECCCCCCCCCHHEECEEEECCEEEEEECEEE
SGLNHTYHDISKPIVKQGITTSPVIIEDETWIGANTVITSGVHIGKHCVIGAGSVVTKDI
ECCCCHHHHHHHHHHHCCCCCCCEEEECCCEECCCCEEECCEEECCEEEEECCCEEECCC
PDYSVAVGNPAKVVKHYDPCGQQWKRTR
CCCEEEECCHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MNISDLKQKIKKNDRLKRTVHYLIMNQRGSCPRLWVKWFVNPFMLTYGKRSKIRRSAIMN
CCHHHHHHHHHHCHHHHHHHHHHEECCCCCCHHHHHHHHHCHHHEECCCHHHHHHHHHCC
VSPINSFILGQESIIEHYVTIDNGVGHVHIGDHSRIGIHNTIIGPVFIGNQVILAQNITI
CCCCHHHHCCHHHHHHHHEEEECCCCEEEECCCCCCCCCHHEECEEEECCEEEEEECEEE
SGLNHTYHDISKPIVKQGITTSPVIIEDETWIGANTVITSGVHIGKHCVIGAGSVVTKDI
ECCCCHHHHHHHHHHHCCCCCCCEEEECCCEECCCCEEECCEEECCEEEEECCCEEECCC
PDYSVAVGNPAKVVKHYDPCGQQWKRTR
CCCEEEECCHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]