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The map label for this gene is grpE

Identifier: 150007881

GI number: 150007881

Start: 1439604

End: 1440188

Strand: Reverse

Name: grpE

Synonym: BDI_1241

Alternate gene names: 150007881

Gene position: 1440188-1439604 (Counterclockwise)

Preceding gene: 150007885

Following gene: 150007880

Centisome position: 29.93

GC content: 48.03

Gene sequence:

>585_bases
ATGAGCAAGATGAATCCAAACGAGAAAAAAGAAAACGCTTCAAAAAACGAAAACGTGAATAATGAGGAAGCGACAAATTT
GCAGGAAGAACAGTCAAATGCGGCAGATGAAGCTGCCGGCTCTGACAATGTGTCGGGTGAGGTGGAAGCCTTGCAAAAGA
AATATAACGAATTGAACGATTCCCACCTGCGCTTGATGGCAGAGTTTGACAATTACCGTAAACGTACGATGCGTGAGAAG
GCCGATTTGATCAAGACTGGCGGCGAGGGTGCGTTAAAGAACCTTCTTCCTATTATCGACGATTTCGAGCGTGCCTTGCA
GAACGTCCGTGCGGCGGAGGATGTAGAGGCCGTTAAAGAAGGCGTGGACTTGATTTTCGGTAAGTTCATGGGCTATCTGT
CTCAGCAGGGCGTGAAGCCGATCGAGGCCATAGGCAAGCCGTTCGATACGGAGGAGTTTGAGGCTATCGCTACGATTCCC
GCTCCGGAACCGGACATGAAGGGCAAGGTATTGGATTGCGTACAAACTGGATATACCTTATTTGACAAGGTAATACGTCA
CGCTAAAGTAGTAGTGGGAGAATAA

Upstream 100 bases:

>100_bases
TGAACTTGATATGGATGCTGTGTATATACTTTTTAAGGGTATTTATGTGGTAACGTATTTCGCTTACAATTAGTTAGTAA
TGAAAAAAATCAGATAGATT

Downstream 100 bases:

>100_bases
TCGGAAAATCGGCAAATAACAAATACAAGGATGGCTAAAAGGGATTACTATGAAGTGCTGGGCGTTGAGAAGAATGCTTC
GGCGGATGAGATAAAGAAAG

Product: molecular chaperon GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSKMNPNEKKENASKNENVNNEEATNLQEEQSNAADEAAGSDNVSGEVEALQKKYNELNDSHLRLMAEFDNYRKRTMREK
ADLIKTGGEGALKNLLPIIDDFERALQNVRAAEDVEAVKEGVDLIFGKFMGYLSQQGVKPIEAIGKPFDTEEFEAIATIP
APEPDMKGKVLDCVQTGYTLFDKVIRHAKVVVGE

Sequences:

>Translated_194_residues
MSKMNPNEKKENASKNENVNNEEATNLQEEQSNAADEAAGSDNVSGEVEALQKKYNELNDSHLRLMAEFDNYRKRTMREK
ADLIKTGGEGALKNLLPIIDDFERALQNVRAAEDVEAVKEGVDLIFGKFMGYLSQQGVKPIEAIGKPFDTEEFEAIATIP
APEPDMKGKVLDCVQTGYTLFDKVIRHAKVVVGE
>Mature_193_residues
SKMNPNEKKENASKNENVNNEEATNLQEEQSNAADEAAGSDNVSGEVEALQKKYNELNDSHLRLMAEFDNYRKRTMREKA
DLIKTGGEGALKNLLPIIDDFERALQNVRAAEDVEAVKEGVDLIFGKFMGYLSQQGVKPIEAIGKPFDTEEFEAIATIPA
PEPDMKGKVLDCVQTGYTLFDKVIRHAKVVVGE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=137, Percent_Identity=35.036496350365, Blast_Score=68, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324806, Length=187, Percent_Identity=34.2245989304813, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PARD8 (A6LBD8)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302624.1
- ProteinModelPortal:   A6LBD8
- SMR:   A6LBD8
- STRING:   A6LBD8
- GeneID:   5306394
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1241
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- BioCyc:   PDIS435591:BDI_1241-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21549; Mature: 21418

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMNPNEKKENASKNENVNNEEATNLQEEQSNAADEAAGSDNVSGEVEALQKKYNELND
CCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCH
SHLRLMAEFDNYRKRTMREKADLIKTGGEGALKNLLPIIDDFERALQNVRAAEDVEAVKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVDLIFGKFMGYLSQQGVKPIEAIGKPFDTEEFEAIATIPAPEPDMKGKVLDCVQTGYTL
HHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHHHH
FDKVIRHAKVVVGE
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SKMNPNEKKENASKNENVNNEEATNLQEEQSNAADEAAGSDNVSGEVEALQKKYNELND
CCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCH
SHLRLMAEFDNYRKRTMREKADLIKTGGEGALKNLLPIIDDFERALQNVRAAEDVEAVKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVDLIFGKFMGYLSQQGVKPIEAIGKPFDTEEFEAIATIPAPEPDMKGKVLDCVQTGYTL
HHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHHHH
FDKVIRHAKVVVGE
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA