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The map label for this gene is punA [H]

Identifier: 150007730

GI number: 150007730

Start: 1262061

End: 1262885

Strand: Direct

Name: punA [H]

Synonym: BDI_1085

Alternate gene names: 150007730

Gene position: 1262061-1262885 (Clockwise)

Preceding gene: 150007729

Following gene: 150007732

Centisome position: 26.23

GC content: 45.94

Gene sequence:

>825_bases
ATGGAAATGATTAAAACCGAACAATATCGGGAAGCGGCCGCCTATTTAGCTAGCCGGATCGAAGGTAACCCGGAGACCGC
CATTATCCTTGGTAGTGGCTTAGGCTCTTTTGCCGATCAAATAATAGACCCTATAGTGATACCTTATGTGGAGATCCCAC
ATTTTATGAAATCTACCGCTACCGGGCATAAGGGGAATTTTATTTGTGGCAAACTAGGGGATAAGCAAGTCTTGGCTATG
CAAGGCCGTTTTCATTACTATGAGGGTTACACGATGCAACAGGTGACTTTCCCCGTACGTGTGATGAAACTCTTAGGAAT
CAAGAACCTTTTGGTCTCGAACGCCGCCGGAGGTATCAATAATACGTTCAAGGTAGGTGATCTGATGATCATCCGGGATC
ATATCAATATGATGCCGAACCCATTGATCGGTCCGAACAATGAGGATTTCGGCACTCGTTTCCCGGATATGACACGTGCT
TATGATCGTGAATTTATCCGCTATGTGGAAGAAATAGCCGCCTCCCGATCTATCCCGTTAAAGAAAGGTGTATATGTAGG
TTTGACAGGCCCTTCCTTCGAGACTCCTTCCGAGTACGCTTTCTATGGAAAAGTCGGGGGAGATGCGATCGGCATGAGTA
CGGTACCGGAAGTAATCGTGGCACGTCATGCGGGCTTGCGTGTATTCGGTATGTCTGTCATCACGAACGAAGGTTATCAC
TTCGCTGACGACTTTGTAAATGATGGTGCCGATGTTATCGTAGCGGCTAATAAAGCAGCATCTGTTATGACACAACTATT
TAGGGAATTGGTCAAAAAAATCTAA

Upstream 100 bases:

>100_bases
AAATATTGATGGAGAATAAGAAGGTACCGGGAGAGGACGAGATTATGGAGAAAGCGGCAGAGATAGCTTATAACTTGATG
AAACGTTAAATTGATTAAAT

Downstream 100 bases:

>100_bases
TAAAAAAGTAGCGCAAAGGCTTGGTGGTTTCAATGAAATCACTACCTTTGCACTCGCAATCGAGAACAAAGCGGTTGCAT
ATGCGGAAGTAGCTCAGTTG

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MEMIKTEQYREAAAYLASRIEGNPETAIILGSGLGSFADQIIDPIVIPYVEIPHFMKSTATGHKGNFICGKLGDKQVLAM
QGRFHYYEGYTMQQVTFPVRVMKLLGIKNLLVSNAAGGINNTFKVGDLMIIRDHINMMPNPLIGPNNEDFGTRFPDMTRA
YDREFIRYVEEIAASRSIPLKKGVYVGLTGPSFETPSEYAFYGKVGGDAIGMSTVPEVIVARHAGLRVFGMSVITNEGYH
FADDFVNDGADVIVAANKAASVMTQLFRELVKKI

Sequences:

>Translated_274_residues
MEMIKTEQYREAAAYLASRIEGNPETAIILGSGLGSFADQIIDPIVIPYVEIPHFMKSTATGHKGNFICGKLGDKQVLAM
QGRFHYYEGYTMQQVTFPVRVMKLLGIKNLLVSNAAGGINNTFKVGDLMIIRDHINMMPNPLIGPNNEDFGTRFPDMTRA
YDREFIRYVEEIAASRSIPLKKGVYVGLTGPSFETPSEYAFYGKVGGDAIGMSTVPEVIVARHAGLRVFGMSVITNEGYH
FADDFVNDGADVIVAANKAASVMTQLFRELVKKI
>Mature_274_residues
MEMIKTEQYREAAAYLASRIEGNPETAIILGSGLGSFADQIIDPIVIPYVEIPHFMKSTATGHKGNFICGKLGDKQVLAM
QGRFHYYEGYTMQQVTFPVRVMKLLGIKNLLVSNAAGGINNTFKVGDLMIIRDHINMMPNPLIGPNNEDFGTRFPDMTRA
YDREFIRYVEEIAASRSIPLKKGVYVGLTGPSFETPSEYAFYGKVGGDAIGMSTVPEVIVARHAGLRVFGMSVITNEGYH
FADDFVNDGADVIVAANKAASVMTQLFRELVKKI

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=269, Percent_Identity=47.2118959107807, Blast_Score=244, Evalue=9e-65,
Organism=Escherichia coli, GI1788746, Length=253, Percent_Identity=40.3162055335968, Blast_Score=190, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17541190, Length=257, Percent_Identity=42.4124513618677, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323238, Length=261, Percent_Identity=42.1455938697318, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI45552887, Length=277, Percent_Identity=42.5992779783394, Blast_Score=216, Evalue=9e-57,
Organism=Drosophila melanogaster, GI45552885, Length=277, Percent_Identity=42.5992779783394, Blast_Score=216, Evalue=9e-57,
Organism=Drosophila melanogaster, GI24656093, Length=260, Percent_Identity=44.2307692307692, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24656090, Length=260, Percent_Identity=44.2307692307692, Blast_Score=216, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24762376, Length=236, Percent_Identity=32.6271186440678, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 30188; Mature: 30188

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMIKTEQYREAAAYLASRIEGNPETAIILGSGLGSFADQIIDPIVIPYVEIPHFMKSTA
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCHHCCHHHHHHHHHHCC
TGHKGNFICGKLGDKQVLAMQGRFHYYEGYTMQQVTFPVRVMKLLGIKNLLVSNAAGGIN
CCCCCCEEEECCCCCEEEEEECCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTFKVGDLMIIRDHINMMPNPLIGPNNEDFGTRFPDMTRAYDREFIRYVEEIAASRSIPL
CCEEECCEEEEECHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KKGVYVGLTGPSFETPSEYAFYGKVGGDAIGMSTVPEVIVARHAGLRVFGMSVITNEGYH
CCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCE
FADDFVNDGADVIVAANKAASVMTQLFRELVKKI
EHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEMIKTEQYREAAAYLASRIEGNPETAIILGSGLGSFADQIIDPIVIPYVEIPHFMKSTA
CCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCHHCCHHHHHHHHHHCC
TGHKGNFICGKLGDKQVLAMQGRFHYYEGYTMQQVTFPVRVMKLLGIKNLLVSNAAGGIN
CCCCCCEEEECCCCCEEEEEECCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
NTFKVGDLMIIRDHINMMPNPLIGPNNEDFGTRFPDMTRAYDREFIRYVEEIAASRSIPL
CCEEECCEEEEECHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KKGVYVGLTGPSFETPSEYAFYGKVGGDAIGMSTVPEVIVARHAGLRVFGMSVITNEGYH
CCCEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCE
FADDFVNDGADVIVAANKAASVMTQLFRELVKKI
EHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]