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The map label for this gene is acnA [H]

Identifier: 150007719

GI number: 150007719

Start: 1249252

End: 1251501

Strand: Direct

Name: acnA [H]

Synonym: BDI_1074

Alternate gene names: 150007719

Gene position: 1249252-1251501 (Clockwise)

Preceding gene: 150007718

Following gene: 150007720

Centisome position: 25.96

GC content: 47.38

Gene sequence:

>2250_bases
ATGGTGTACGACATTGACATGCTTAGAAATTTCTATGCGAATTTCCCCAGAAGAGTGGACACAGCCCGCGAACGTATCGG
TGGTCGCCCGATGACCTTGGCCGAGAAAATCTTATATGCGCATCTATACGATGAAAGCTCGACTCGCCTTTTCAAAAGAG
GTGAGGATTATGTGAATTTCCGTCCCGACCGTGTGGCTATGCAAGATGCCACCGCTCAGATGGCGCTTTTACAATTCATG
AACGCCGGAAAAGAAAAATCCGCAGTACCGGCTACCGTGCATTGCGACCATTTGATCCAAGCGAATATGGGAGCCAAAAC
GGATATCGATACGGCTACCAAGAGCAATAGCGAGGTCTATGATTTCTTGAAATCGGTCTCCGATAAATATGGAATCGGTT
TTTGGAAACCCGGAGCAGGCATTATCCATCAAGTGGTATTGGAAAATTATGCGTTTCCCGGCGGTATGATGGTAGGAACT
GATTCACATACCCCGAATGCCGGAGGACTTGGAATGGTCGCTATCGGAGTAGGAGGAGCCGATGCCGTGGATGTCATGAC
CGGAATGGAATGGGAACTGAAGATGCCGAAACTAATTGGTGTTAAGTTAACTGGTAGTTTGAATGGTTGGGCCTCGCCGA
AGGACGTGATTCTTAAGCTAGCGGGCATCTTAACCGTAAAAGGTGGAACGAACGCTATCATCGAATATTTCGGTCCGGGC
ACCGCCTCAATCTCAGCGACCGGAAAAGCTACGATCTGTAATATGGGTGCCGAGGTAGGAGCGACTACTTCCTTATTCCC
ATTCGATAATACAATGGCTCAATATCTTCGTGCCACGGGACGTGACGAGGTAGCGAGCTGGGCCGAAGCTATCGGTGAAT
ATCTGGAAGCGGATATGGACGTTCGTGCCGAGCCAGATAAGTTTTACGATCGTGTAATCGTTATTAACCTTTCCGAATTG
GAGCCGCATATCAACGGTCCGTTCACGCCGGATGCAGCTACGCCTATCTCGGAGTTTGCCGCAAAAGTAAAAGCGAATGG
CTATCCCCGAAAGATGGAGGTCGGATTAATCGGTTCCTGTACCAACTCTTCTTATCAAGATCTAAGCCGTGCGGCATCTA
TTGCTCGCCAAGCTTATGAGGATAAAATACCGGTAGCGGCACCCTTGATCATCAACCCGGGTTCCGAGCAAATCCGTTAT
ACGGCAGAAAGAGATGGTATTATCGGTGATTTTGAACGGATTGGCGCAACCATTATGGCGAATGCCTGTGGTCCTTGTAT
CGGACAATGGAAACGTCATACGGATGATAATACCCGTAAGAATTCGATCGTTACTTCATTCAATCGTAACTTCGCTAAAC
GTGCGGACGGTAATCCGAATACACATGCTTTTGTCGCTTCTCCGGAACTGACACTGGCCTTGACCATCGCAGGTGATCTA
TGTTTCAATCCTTTGACCGATACCTTGAAGACAGAGGATGGTAAGGTTGTGAAACTGAAAGAACCGAAAGGCTCGGATTT
TCCGCCGAAAGGTTTCGAGGTAAAGGATAACGGCTATCTGGCACCTACGGGTAAGAACGTAGTGGTGAACATCGATCCGG
AATCCAACCGCCTACAAGCATTAAAGCCTTTCGCTCCTTGGAATGGAGAGGATTTCACGGATATGCCCCTTCTTATCAAG
GCTGAAGGTAAATGTACGACGGATCATATCTCGATGGCCGGTCCATGGTTACGTTTCCGTGGGCATTTGGAAAATATATC
GGATAATATGTTGATGGGAGCCGTAAACGCTTTCAACGGGAAGACAAACTCTGTATTGAATCAACTGAACAATGAATATG
AAGCAGTTTCTGCCGTAGCGAAACAATACAAGGCAAATAGTATTTCCTCGATCGTCGTAGCGGAAGAAAACTACGGTGAA
GGCTCTTCCCGGGAACATGCAGCCATGGAGCCCCGCTTCCTAAATGTAAAAGTGATCCTAGCCAAGAGTTTCGCCCGTAT
CCACGAGACAAACTTGAAGAAACAAGGAATGTTAGCACTGACTTTTGCCAATAAGGAGGATTACAGCAAGATCCGCGAGA
ATGACCATATCTCGATCATCGGATTGAAAGATTTTCAGCCGGGCAAACCGTTGACGGCCGTTCTTCTTCATGCGGATGGT
ACGCAAGAATCATTCCCGGTTAACCATACGTATAATGATTTACAAATTAAATGGTTCAAGGCGGGTTCCGCTTTAAATAC
AGCTCACTAA

Upstream 100 bases:

>100_bases
AAACAAAGACTCATTGGCAGATGTCTTATTTATTACATTTTAGCTGGTTATAGATAAATATATTTGCGAAAAGGCTTACA
ATAAAAAACGAATATGAATT

Downstream 100 bases:

>100_bases
GACAAACAAAAAAATCTTGATATCATGAGTAAGATAACAAAGAAAGGTAATCATATTTTCGTTCCGGATCACGTGACGAT
CCCTTATATTGAAGGAGATG

Product: aconitate hydratase

Products: NA

Alternate protein names: Aconitase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 749; Mature: 749

Protein sequence:

>749_residues
MVYDIDMLRNFYANFPRRVDTARERIGGRPMTLAEKILYAHLYDESSTRLFKRGEDYVNFRPDRVAMQDATAQMALLQFM
NAGKEKSAVPATVHCDHLIQANMGAKTDIDTATKSNSEVYDFLKSVSDKYGIGFWKPGAGIIHQVVLENYAFPGGMMVGT
DSHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPKLIGVKLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYFGPG
TASISATGKATICNMGAEVGATTSLFPFDNTMAQYLRATGRDEVASWAEAIGEYLEADMDVRAEPDKFYDRVIVINLSEL
EPHINGPFTPDAATPISEFAAKVKANGYPRKMEVGLIGSCTNSSYQDLSRAASIARQAYEDKIPVAAPLIINPGSEQIRY
TAERDGIIGDFERIGATIMANACGPCIGQWKRHTDDNTRKNSIVTSFNRNFAKRADGNPNTHAFVASPELTLALTIAGDL
CFNPLTDTLKTEDGKVVKLKEPKGSDFPPKGFEVKDNGYLAPTGKNVVVNIDPESNRLQALKPFAPWNGEDFTDMPLLIK
AEGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGKTNSVLNQLNNEYEAVSAVAKQYKANSISSIVVAEENYGE
GSSREHAAMEPRFLNVKVILAKSFARIHETNLKKQGMLALTFANKEDYSKIRENDHISIIGLKDFQPGKPLTAVLLHADG
TQESFPVNHTYNDLQIKWFKAGSALNTAH

Sequences:

>Translated_749_residues
MVYDIDMLRNFYANFPRRVDTARERIGGRPMTLAEKILYAHLYDESSTRLFKRGEDYVNFRPDRVAMQDATAQMALLQFM
NAGKEKSAVPATVHCDHLIQANMGAKTDIDTATKSNSEVYDFLKSVSDKYGIGFWKPGAGIIHQVVLENYAFPGGMMVGT
DSHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPKLIGVKLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYFGPG
TASISATGKATICNMGAEVGATTSLFPFDNTMAQYLRATGRDEVASWAEAIGEYLEADMDVRAEPDKFYDRVIVINLSEL
EPHINGPFTPDAATPISEFAAKVKANGYPRKMEVGLIGSCTNSSYQDLSRAASIARQAYEDKIPVAAPLIINPGSEQIRY
TAERDGIIGDFERIGATIMANACGPCIGQWKRHTDDNTRKNSIVTSFNRNFAKRADGNPNTHAFVASPELTLALTIAGDL
CFNPLTDTLKTEDGKVVKLKEPKGSDFPPKGFEVKDNGYLAPTGKNVVVNIDPESNRLQALKPFAPWNGEDFTDMPLLIK
AEGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGKTNSVLNQLNNEYEAVSAVAKQYKANSISSIVVAEENYGE
GSSREHAAMEPRFLNVKVILAKSFARIHETNLKKQGMLALTFANKEDYSKIRENDHISIIGLKDFQPGKPLTAVLLHADG
TQESFPVNHTYNDLQIKWFKAGSALNTAH
>Mature_749_residues
MVYDIDMLRNFYANFPRRVDTARERIGGRPMTLAEKILYAHLYDESSTRLFKRGEDYVNFRPDRVAMQDATAQMALLQFM
NAGKEKSAVPATVHCDHLIQANMGAKTDIDTATKSNSEVYDFLKSVSDKYGIGFWKPGAGIIHQVVLENYAFPGGMMVGT
DSHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPKLIGVKLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYFGPG
TASISATGKATICNMGAEVGATTSLFPFDNTMAQYLRATGRDEVASWAEAIGEYLEADMDVRAEPDKFYDRVIVINLSEL
EPHINGPFTPDAATPISEFAAKVKANGYPRKMEVGLIGSCTNSSYQDLSRAASIARQAYEDKIPVAAPLIINPGSEQIRY
TAERDGIIGDFERIGATIMANACGPCIGQWKRHTDDNTRKNSIVTSFNRNFAKRADGNPNTHAFVASPELTLALTIAGDL
CFNPLTDTLKTEDGKVVKLKEPKGSDFPPKGFEVKDNGYLAPTGKNVVVNIDPESNRLQALKPFAPWNGEDFTDMPLLIK
AEGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGKTNSVLNQLNNEYEAVSAVAKQYKANSISSIVVAEENYGE
GSSREHAAMEPRFLNVKVILAKSFARIHETNLKKQGMLALTFANKEDYSKIRENDHISIIGLKDFQPGKPLTAVLLHADG
TQESFPVNHTYNDLQIKWFKAGSALNTAH

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=739, Percent_Identity=56.8335588633288, Blast_Score=859, Evalue=0.0,
Organism=Homo sapiens, GI8659555, Length=827, Percent_Identity=27.4486094316808, Blast_Score=217, Evalue=4e-56,
Organism=Homo sapiens, GI41352693, Length=446, Percent_Identity=29.1479820627803, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI1787531, Length=797, Percent_Identity=26.0978670012547, Blast_Score=169, Evalue=6e-43,
Organism=Escherichia coli, GI87081781, Length=433, Percent_Identity=24.7113163972286, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI1786259, Length=402, Percent_Identity=25.6218905472637, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25149337, Length=735, Percent_Identity=57.4149659863946, Blast_Score=868, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564738, Length=680, Percent_Identity=58.3823529411765, Blast_Score=820, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25149342, Length=570, Percent_Identity=57.0175438596491, Blast_Score=681, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17568399, Length=787, Percent_Identity=27.3189326556544, Blast_Score=207, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323335, Length=740, Percent_Identity=57.7027027027027, Blast_Score=872, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322261, Length=751, Percent_Identity=52.8628495339547, Blast_Score=780, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320440, Length=435, Percent_Identity=27.3563218390805, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=26.5392781316348, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI281365315, Length=738, Percent_Identity=59.4850948509485, Blast_Score=870, Evalue=0.0,
Organism=Drosophila melanogaster, GI17864292, Length=738, Percent_Identity=59.4850948509485, Blast_Score=870, Evalue=0.0,
Organism=Drosophila melanogaster, GI28571643, Length=765, Percent_Identity=56.7320261437908, Blast_Score=858, Evalue=0.0,
Organism=Drosophila melanogaster, GI161076999, Length=683, Percent_Identity=60.7613469985359, Blast_Score=820, Evalue=0.0,
Organism=Drosophila melanogaster, GI24645686, Length=835, Percent_Identity=27.6646706586826, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17137564, Length=834, Percent_Identity=27.0983213429257, Blast_Score=196, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR006249
- InterPro:   IPR015934
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00330 Aconitase; PF00694 Aconitase_C [H]

EC number: =4.2.1.3 [H]

Molecular weight: Translated: 81750; Mature: 81750

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYDIDMLRNFYANFPRRVDTARERIGGRPMTLAEKILYAHLYDESSTRLFKRGEDYVNF
CEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
RPDRVAMQDATAQMALLQFMNAGKEKSAVPATVHCDHLIQANMGAKTDIDTATKSNSEVY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHH
DFLKSVSDKYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTDSHTPNAGGLGMVAIGVGGA
HHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCC
DAVDVMTGMEWELKMPKLIGVKLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYFGPG
CHHHHHCCCCEEEECCEEEEEEEECCCCCCCCHHHHHHHHHHEEEECCCCCEEEEEECCC
TASISATGKATICNMGAEVGATTSLFPFDNTMAQYLRATGRDEVASWAEAIGEYLEADMD
CCEEECCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
VRAEPDKFYDRVIVINLSELEPHINGPFTPDAATPISEFAAKVKANGYPRKMEVGLIGSC
CCCCCHHHHCEEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECC
TNSSYQDLSRAASIARQAYEDKIPVAAPLIINPGSEQIRYTAERDGIIGDFERIGATIMA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHCHHEEC
NACGPCIGQWKRHTDDNTRKNSIVTSFNRNFAKRADGNPNTHAFVASPELTLALTIAGDL
CCCCHHHHHHHHCCCCCCCCCCEEEHHCCCHHHHCCCCCCCEEEEECCCEEEEEEEECCH
CFNPLTDTLKTEDGKVVKLKEPKGSDFPPKGFEVKDNGYLAPTGKNVVVNIDPESNRLQA
HCCCCHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCCEECCCCCEEEEEECCCCCCEEE
LKPFAPWNGEDFTDMPLLIKAEGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNG
ECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHCCC
KTNSVLNQLNNEYEAVSAVAKQYKANSISSIVVAEENYGEGSSREHAAMEPRFLNVKVIL
CHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEE
AKSFARIHETNLKKQGMLALTFANKEDYSKIRENDHISIIGLKDFQPGKPLTAVLLHADG
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCC
TQESFPVNHTYNDLQIKWFKAGSALNTAH
CCCCCCCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MVYDIDMLRNFYANFPRRVDTARERIGGRPMTLAEKILYAHLYDESSTRLFKRGEDYVNF
CEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
RPDRVAMQDATAQMALLQFMNAGKEKSAVPATVHCDHLIQANMGAKTDIDTATKSNSEVY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHH
DFLKSVSDKYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTDSHTPNAGGLGMVAIGVGGA
HHHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCC
DAVDVMTGMEWELKMPKLIGVKLTGSLNGWASPKDVILKLAGILTVKGGTNAIIEYFGPG
CHHHHHCCCCEEEECCEEEEEEEECCCCCCCCHHHHHHHHHHEEEECCCCCEEEEEECCC
TASISATGKATICNMGAEVGATTSLFPFDNTMAQYLRATGRDEVASWAEAIGEYLEADMD
CCEEECCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
VRAEPDKFYDRVIVINLSELEPHINGPFTPDAATPISEFAAKVKANGYPRKMEVGLIGSC
CCCCCHHHHCEEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEEECC
TNSSYQDLSRAASIARQAYEDKIPVAAPLIINPGSEQIRYTAERDGIIGDFERIGATIMA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHCHHEEC
NACGPCIGQWKRHTDDNTRKNSIVTSFNRNFAKRADGNPNTHAFVASPELTLALTIAGDL
CCCCHHHHHHHHCCCCCCCCCCEEEHHCCCHHHHCCCCCCCEEEEECCCEEEEEEEECCH
CFNPLTDTLKTEDGKVVKLKEPKGSDFPPKGFEVKDNGYLAPTGKNVVVNIDPESNRLQA
HCCCCHHHHCCCCCCEEEEECCCCCCCCCCCEEECCCCEECCCCCEEEEEECCCCCCEEE
LKPFAPWNGEDFTDMPLLIKAEGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNG
ECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEHHHCCC
KTNSVLNQLNNEYEAVSAVAKQYKANSISSIVVAEENYGEGSSREHAAMEPRFLNVKVIL
CHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEEEE
AKSFARIHETNLKKQGMLALTFANKEDYSKIRENDHISIIGLKDFQPGKPLTAVLLHADG
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCEEEEEEECCC
TQESFPVNHTYNDLQIKWFKAGSALNTAH
CCCCCCCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA