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The map label for this gene is thiE [H]

Identifier: 150007705

GI number: 150007705

Start: 1230232

End: 1230960

Strand: Reverse

Name: thiE [H]

Synonym: BDI_1060

Alternate gene names: 150007705

Gene position: 1230960-1230232 (Counterclockwise)

Preceding gene: 150007706

Following gene: 150007704

Centisome position: 25.58

GC content: 47.74

Gene sequence:

>729_bases
ATGGCCTTAAATTATTATTTCGGATATGATGAGAATGGAAGAGTCGATCGGAGAACAACCGGATTACGCCCCCCTTTTGG
TGGAAACAATCGCTTGATGTTTATAACGCATCGTACGCCTAAATATACGGAATGCGACGAGGTAAGCATGGCAATCCAAG
GAGGGTGCTCATGGATACAGTTGCGCATGAAGGATGGGATTTATGAGGATACCGTACGTACATGTGCAACTATCTGCGCG
GAAGAATGTGAGCGAATCGTGGATTTCTGCGTAAACGATGATCTGGAGGCTGCCGTGACATGTGGCGCTACGGCTTGTCA
TCTGGGTAAGAATGATATGCCCTTGGATATCGCTTGGGAAGTACTGAGAGACAAGTTAGATTCCAATGCCATCTTCTATA
TCGGGGCTACGGCGAATACCTTTGAGGATATCCGGCTGGCCGTAGAGCGAGGCGCCTCGTATATCGGACTAGGGCCGTAT
CGCTTTACGGGTACCAAGAAGAATTTGAGTCCCATCTTAGGCTTGGATGGTTATCGTAAAATAATCGCTCAATGCAAGGA
GGCTGACATTGATATTCCTATCTTCGCGATAGGTGGTATTACGCTGGAAGACGTAGGCCCTTTGATGGAGACAGGTATAA
CCGGAATCGCTGTTTCGGGCGCGATTATCAATGCTTCCGATCCCGTGGAGGAAACCCGCCGGTTTATAGAGGAAATCAAT
AAGTACTAA

Upstream 100 bases:

>100_bases
TCCCCTTCGGCGGTGTCGCCGTTCTCGGGGCCCTTTGGGGAAACGATCCCTCCCTTTTTGTTGCGGATCAACTCATAAAA
CAATTCAAACGATTACAAGT

Downstream 100 bases:

>100_bases
ACTAAATACATATGGCTAATAAAAAGAATATGCGTATTTCCTATCCGTCCTCGGAAAAGATTTATGTCCCGGGCGAGATC
AATAAGATCAAGGTTGGTAT

Product: putative thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MALNYYFGYDENGRVDRRTTGLRPPFGGNNRLMFITHRTPKYTECDEVSMAIQGGCSWIQLRMKDGIYEDTVRTCATICA
EECERIVDFCVNDDLEAAVTCGATACHLGKNDMPLDIAWEVLRDKLDSNAIFYIGATANTFEDIRLAVERGASYIGLGPY
RFTGTKKNLSPILGLDGYRKIIAQCKEADIDIPIFAIGGITLEDVGPLMETGITGIAVSGAIINASDPVEETRRFIEEIN
KY

Sequences:

>Translated_242_residues
MALNYYFGYDENGRVDRRTTGLRPPFGGNNRLMFITHRTPKYTECDEVSMAIQGGCSWIQLRMKDGIYEDTVRTCATICA
EECERIVDFCVNDDLEAAVTCGATACHLGKNDMPLDIAWEVLRDKLDSNAIFYIGATANTFEDIRLAVERGASYIGLGPY
RFTGTKKNLSPILGLDGYRKIIAQCKEADIDIPIFAIGGITLEDVGPLMETGITGIAVSGAIINASDPVEETRRFIEEIN
KY
>Mature_241_residues
ALNYYFGYDENGRVDRRTTGLRPPFGGNNRLMFITHRTPKYTECDEVSMAIQGGCSWIQLRMKDGIYEDTVRTCATICAE
ECERIVDFCVNDDLEAAVTCGATACHLGKNDMPLDIAWEVLRDKLDSNAIFYIGATANTFEDIRLAVERGASYIGLGPYR
FTGTKKNLSPILGLDGYRKIIAQCKEADIDIPIFAIGGITLEDVGPLMETGITGIAVSGAIINASDPVEETRRFIEEINK
Y

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 26792; Mature: 26661

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNYYFGYDENGRVDRRTTGLRPPFGGNNRLMFITHRTPKYTECDEVSMAIQGGCSWIQ
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEE
LRMKDGIYEDTVRTCATICAEECERIVDFCVNDDLEAAVTCGATACHLGKNDMPLDIAWE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCCCCCHHHHHH
VLRDKLDSNAIFYIGATANTFEDIRLAVERGASYIGLGPYRFTGTKKNLSPILGLDGYRK
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCEEECCCCCCCCHHCCCCHHHH
IIAQCKEADIDIPIFAIGGITLEDVGPLMETGITGIAVSGAIINASDPVEETRRFIEEIN
HHHHHHHCCCCCEEEEECCEEHHHHHHHHHCCCCEEEEECEEECCCCCHHHHHHHHHHHH
KY
CC
>Mature Secondary Structure 
ALNYYFGYDENGRVDRRTTGLRPPFGGNNRLMFITHRTPKYTECDEVSMAIQGGCSWIQ
EEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCEEEE
LRMKDGIYEDTVRTCATICAEECERIVDFCVNDDLEAAVTCGATACHLGKNDMPLDIAWE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCCCCCHHHHHH
VLRDKLDSNAIFYIGATANTFEDIRLAVERGASYIGLGPYRFTGTKKNLSPILGLDGYRK
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCEEECCCEEECCCCCCCCHHCCCCHHHH
IIAQCKEADIDIPIFAIGGITLEDVGPLMETGITGIAVSGAIINASDPVEETRRFIEEIN
HHHHHHHCCCCCEEEEECCEEHHHHHHHHHCCCCEEEEECEEECCCCCHHHHHHHHHHHH
KY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA