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The map label for this gene is thiC [H]

Identifier: 150007704

GI number: 150007704

Start: 1228523

End: 1230220

Strand: Reverse

Name: thiC [H]

Synonym: BDI_1059

Alternate gene names: 150007704

Gene position: 1230220-1228523 (Counterclockwise)

Preceding gene: 150007705

Following gene: 150007701

Centisome position: 25.57

GC content: 50.41

Gene sequence:

>1698_bases
ATGGCTAATAAAAAGAATATGCGTATTTCCTATCCGTCCTCGGAAAAGATTTATGTCCCGGGCGAGATCAATAAGATCAA
GGTTGGTATGCGTAAGATTAAATTGTTGGACACGGTAACCTTGGATGAGCATGGAGAACGAATCTTCAAGAAGAACAATC
CGGTAATCGTTTACGATACGAGCGGCCCGTATTCCGATCCCAAGATATCGATTGATATCGCTAAAGGACTTCCTCGTATA
CGTGAGGAATGGTACGGTAAGCGTAAAGATGTGGTTCAATTGCCGGAATTGACTTCCGCTTATGGCCGTGAGCGATTGGC
GGACGCGAGTCTGGATTCCCTGCGTTTCCCCAAGCGATACTTGCCTTTCCGGGCTAAAGAGGGTAAGAATATCACCCAGA
TGTATTATGCGAAGAAGCGCATTATCACTCCGGAAATGGAGTACGTGGCGATCCGTGAGAACCAGCAAATCGAGGCTTTG
GGACTGAAGTCGTATATCACGCCGGAGTTCGTCCGCAAGGAAATCGCCGCCGGTCGCGCGATCATCCCGGCGAATATCAA
TCACCCGGAAGCCGAGCCGATGATTATCGGTAAGAAATTCTTGGTAAAGATCAATACCAATATCGGTAACTCCGCTCTTT
CCTCCGGTATCGACGAGGAGATCGAGAAGGCTATATGGAGTTGTAAATGGGGCGGAGACACGTTGATGGATCTCTCTACC
GGCGATCATATCCATGAGACGCGCGAATGGATTATCCGTAACTGCCCGGTTCCGATGGGTACGGTTCCTATTTATCAAGC
CTTGGAGAAAGTGAATGGTAAGGTAGAGGATCTTTCTTGGGAGATCTATCGGGATACGTTGGTCGAGCAGGCGGAACAGG
GCGTGGATTATTTCACGATCCACGCGGGTTTGTTGCGCAAGCATGTGGAGCTGACGGCGACTCGCCTGACGGGGATCGTA
TCCAGAGGCGGATCGATCATGGCCAAATGGATGCAGCTTCATGACGAGGAAAACTTCCTGTACACGCATTTCGCCGAGAT
CTGCGAGATACTGAAGGCATACGACGTGGCGGTCTCTATCGGAGACGGGCTTCGTCCCGGTTCTATCTATGACGCGAACG
ACGCGGCACAATTCGCCGAGCTTCACACGATGGGAGAACTGACGAAAATAGCTTGGGAGCAGTTCGTACAAGTGATTATC
GAAGGCCCGGGCCATGTTCCCATGAATAAGATTCATGAGAATATGAAGGAACAACAGTATGCCTGTCATGGAGCGCCGTT
CTATACGCTGGGCCCGTTGACGACCGATATCGCTCCTGGATACGATCATATCACGTCGGCTATCGGTGGCGCGCAAATTG
CTTGGCATGGGACGGCCATGATCTGCTACGTTACGCCGAAGGAGCATTTGGGATTGCCGAATAAGGAAGACGTGCGTAAT
GGGGTGGTCGCTTATAAGATAGCGGCGCATGCCGCTGACTTGGCGAAAGGGCATCCCGGCGCTCAAGTACGTGATAACGC
GTTGAGCAAGGCACGTTTTGATTTCCGCTGGAAAGACCAGTTCAATTTGTCCTTGGACCCGGAAAGGGCTTTACAGTATT
ATAAGGAGAGTGCTGTTACGGATGGAGAGTACTGTACGATGTGCGGTCCTAATTTCTGCGCTATGCGGTTAAGTAAGGAT
TTGCAAGGTTGTAAATAA

Upstream 100 bases:

>100_bases
CCGGAATCGCTGTTTCGGGCGCGATTATCAATGCTTCCGATCCCGTGGAGGAAACCCGCCGGTTTATAGAGGAAATCAAT
AAGTACTAAACTAAATACAT

Downstream 100 bases:

>100_bases
AATACTGTTTTGAGGGGATGAGACGGGGCATGCCCCGTCTCTACAATTGAGTGATTTGTTTTCCGCATTCGTAGCCTTCC
TCATATAGTTCTTCCAGTTT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MANKKNMRISYPSSEKIYVPGEINKIKVGMRKIKLLDTVTLDEHGERIFKKNNPVIVYDTSGPYSDPKISIDIAKGLPRI
REEWYGKRKDVVQLPELTSAYGRERLADASLDSLRFPKRYLPFRAKEGKNITQMYYAKKRIITPEMEYVAIRENQQIEAL
GLKSYITPEFVRKEIAAGRAIIPANINHPEAEPMIIGKKFLVKINTNIGNSALSSGIDEEIEKAIWSCKWGGDTLMDLST
GDHIHETREWIIRNCPVPMGTVPIYQALEKVNGKVEDLSWEIYRDTLVEQAEQGVDYFTIHAGLLRKHVELTATRLTGIV
SRGGSIMAKWMQLHDEENFLYTHFAEICEILKAYDVAVSIGDGLRPGSIYDANDAAQFAELHTMGELTKIAWEQFVQVII
EGPGHVPMNKIHENMKEQQYACHGAPFYTLGPLTTDIAPGYDHITSAIGGAQIAWHGTAMICYVTPKEHLGLPNKEDVRN
GVVAYKIAAHAADLAKGHPGAQVRDNALSKARFDFRWKDQFNLSLDPERALQYYKESAVTDGEYCTMCGPNFCAMRLSKD
LQGCK

Sequences:

>Translated_565_residues
MANKKNMRISYPSSEKIYVPGEINKIKVGMRKIKLLDTVTLDEHGERIFKKNNPVIVYDTSGPYSDPKISIDIAKGLPRI
REEWYGKRKDVVQLPELTSAYGRERLADASLDSLRFPKRYLPFRAKEGKNITQMYYAKKRIITPEMEYVAIRENQQIEAL
GLKSYITPEFVRKEIAAGRAIIPANINHPEAEPMIIGKKFLVKINTNIGNSALSSGIDEEIEKAIWSCKWGGDTLMDLST
GDHIHETREWIIRNCPVPMGTVPIYQALEKVNGKVEDLSWEIYRDTLVEQAEQGVDYFTIHAGLLRKHVELTATRLTGIV
SRGGSIMAKWMQLHDEENFLYTHFAEICEILKAYDVAVSIGDGLRPGSIYDANDAAQFAELHTMGELTKIAWEQFVQVII
EGPGHVPMNKIHENMKEQQYACHGAPFYTLGPLTTDIAPGYDHITSAIGGAQIAWHGTAMICYVTPKEHLGLPNKEDVRN
GVVAYKIAAHAADLAKGHPGAQVRDNALSKARFDFRWKDQFNLSLDPERALQYYKESAVTDGEYCTMCGPNFCAMRLSKD
LQGCK
>Mature_564_residues
ANKKNMRISYPSSEKIYVPGEINKIKVGMRKIKLLDTVTLDEHGERIFKKNNPVIVYDTSGPYSDPKISIDIAKGLPRIR
EEWYGKRKDVVQLPELTSAYGRERLADASLDSLRFPKRYLPFRAKEGKNITQMYYAKKRIITPEMEYVAIRENQQIEALG
LKSYITPEFVRKEIAAGRAIIPANINHPEAEPMIIGKKFLVKINTNIGNSALSSGIDEEIEKAIWSCKWGGDTLMDLSTG
DHIHETREWIIRNCPVPMGTVPIYQALEKVNGKVEDLSWEIYRDTLVEQAEQGVDYFTIHAGLLRKHVELTATRLTGIVS
RGGSIMAKWMQLHDEENFLYTHFAEICEILKAYDVAVSIGDGLRPGSIYDANDAAQFAELHTMGELTKIAWEQFVQVIIE
GPGHVPMNKIHENMKEQQYACHGAPFYTLGPLTTDIAPGYDHITSAIGGAQIAWHGTAMICYVTPKEHLGLPNKEDVRNG
VVAYKIAAHAADLAKGHPGAQVRDNALSKARFDFRWKDQFNLSLDPERALQYYKESAVTDGEYCTMCGPNFCAMRLSKDL
QGCK

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=573, Percent_Identity=62.1291448516579, Blast_Score=755, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 63627; Mature: 63495

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKKNMRISYPSSEKIYVPGEINKIKVGMRKIKLLDTVTLDEHGERIFKKNNPVIVYDT
CCCCCCCEEECCCCCEEEECCCCCEEHHHHHHHHHHHEEEHHHHHHHHHHCCCCEEEEEC
SGPYSDPKISIDIAKGLPRIREEWYGKRKDVVQLPELTSAYGRERLADASLDSLRFPKRY
CCCCCCCEEEEEHHHCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHHCCCHHH
LPFRAKEGKNITQMYYAKKRIITPEMEYVAIRENQQIEALGLKSYITPEFVRKEIAAGRA
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCHHHCCHHHHHHHHHCCCE
IIPANINHPEAEPMIIGKKFLVKINTNIGNSALSSGIDEEIEKAIWSCKWGGDTLMDLST
EEECCCCCCCCCCEEECCEEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCCCCEEEECCC
GDHIHETREWIIRNCPVPMGTVPIYQALEKVNGKVEDLSWEIYRDTLVEQAEQGVDYFTI
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
HAGLLRKHVELTATRLTGIVSRGGSIMAKWMQLHDEENFLYTHFAEICEILKAYDVAVSI
EHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEE
GDGLRPGSIYDANDAAQFAELHTMGELTKIAWEQFVQVIIEGPGHVPMNKIHENMKEQQY
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ACHGAPFYTLGPLTTDIAPGYDHITSAIGGAQIAWHGTAMICYVTPKEHLGLPNKEDVRN
HHCCCCCEEECCCCCCCCCCHHHHHHHCCCCEEEEECCEEEEEECCHHHCCCCCHHHHHC
GVVAYKIAAHAADLAKGHPGAQVRDNALSKARFDFRWKDQFNLSLDPERALQYYKESAVT
CEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCCC
DGEYCTMCGPNFCAMRLSKDLQGCK
CCCEEECCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
ANKKNMRISYPSSEKIYVPGEINKIKVGMRKIKLLDTVTLDEHGERIFKKNNPVIVYDT
CCCCCCEEECCCCCEEEECCCCCEEHHHHHHHHHHHEEEHHHHHHHHHHCCCCEEEEEC
SGPYSDPKISIDIAKGLPRIREEWYGKRKDVVQLPELTSAYGRERLADASLDSLRFPKRY
CCCCCCCEEEEEHHHCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHCCHHHHCCCHHH
LPFRAKEGKNITQMYYAKKRIITPEMEYVAIRENQQIEALGLKSYITPEFVRKEIAAGRA
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCHHHCCHHHHHHHHHCCCE
IIPANINHPEAEPMIIGKKFLVKINTNIGNSALSSGIDEEIEKAIWSCKWGGDTLMDLST
EEECCCCCCCCCCEEECCEEEEEEECCCCCHHHHCCCHHHHHHHHHCCCCCCCEEEECCC
GDHIHETREWIIRNCPVPMGTVPIYQALEKVNGKVEDLSWEIYRDTLVEQAEQGVDYFTI
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
HAGLLRKHVELTATRLTGIVSRGGSIMAKWMQLHDEENFLYTHFAEICEILKAYDVAVSI
EHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEE
GDGLRPGSIYDANDAAQFAELHTMGELTKIAWEQFVQVIIEGPGHVPMNKIHENMKEQQY
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ACHGAPFYTLGPLTTDIAPGYDHITSAIGGAQIAWHGTAMICYVTPKEHLGLPNKEDVRN
HHCCCCCEEECCCCCCCCCCHHHHHHHCCCCEEEEECCEEEEEECCHHHCCCCCHHHHHC
GVVAYKIAAHAADLAKGHPGAQVRDNALSKARFDFRWKDQFNLSLDPERALQYYKESAVT
CEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHCCC
DGEYCTMCGPNFCAMRLSKDLQGCK
CCCEEECCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]