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The map label for this gene is nuoF2 [H]

Identifier: 150007688

GI number: 150007688

Start: 1208001

End: 1209791

Strand: Direct

Name: nuoF2 [H]

Synonym: BDI_1043

Alternate gene names: 150007688

Gene position: 1208001-1209791 (Clockwise)

Preceding gene: 150007687

Following gene: 150007689

Centisome position: 25.11

GC content: 47.96

Gene sequence:

>1791_bases
ATGGCACAATACACGAATTATATATTGGTGTGCGGTGGCACAGGTTGCCGTGCATCACAAAGCGAGCAGATCATCGAGAA
TCTGAGAGCCGCCGTCGAGCAATACGGATTAGAGGATACACAAGTGATCCGTACCGGATGCTTCGGTTTCTGCGAGAAGG
GTCCGGTGGTTAAGATGATCCCCGATAACACTTTTTACGTACAAGTTCAACCATCGGATGCGGACGAGATCGTACGGGAG
CATTTGGTAAAAGGCCGTAAAGTGGAACGGTTACTTTATATGAATCCAGAGACCCGTGAGCTGGTTCCGGACTCCAAGCA
TATCAGATTCTATAAGAAACAGCTACGAATCGCCCTTCGAAATTGTGGCTTTATCAACCCTGAGAATATCGATGAGTATA
TCGCTCGTGACGGCTATGTAGCGTTAGGAAAAGCCCTTGTGGAAATGACTCCCGAGGAAGTAATCAAGGAAATCATGGAT
AGCGGTTTACGGGGTCGTGGGGGCGGTGGTTTCCCCACAGGTTTGAAATGGCAGATTACCCGTAAGGTACAAGCACCTCA
AAAATACGTGGTTTGCAATGCGGACGAGGGTGACCCGGGCGCTTTTATGGATCGCTCCATATTGGAGGGCGACCCGCATT
CGATTGTGGAGGCTATGGCGATTAATGGATATTGTACGGGAGCTAGCAAAGGTTTGGTATATATCCGTGCCGAATATCCG
CTAGCCGTGGAACGTTTGAAAATCGCCATACGGCAAGCGAAAGAATATGGATTGCTGGGCGAGAATATATTTGGGACTGA
TTTCTCATTCGATATCGAGATCCGGTACGGTGCCGGAGCTTTTGTTTGTGGAGAGGAGACTGCTTTGATCCACAGTATGG
AAGGTTTGCGAGGCGAGGCTACCGTAAAGCCGCCGTTCCCTAGTGAACACGGGTATAAAGGTTGTCCGACCAATGTAAAT
AACGTAGAGACTTACGCAAATATCCCGGTGATCTTGTTAAGAGGCGCTAAATGGTTCAGTAGTATCGGAACGGAGAAAAG
CAAAGGAACAAAGGTGTTCGCCTTGGCTGGAAAGGTAAACAATGTGGGGCTGATCGAGGTTCCTATGGGTACGACTTTGC
GTGAGGTTATCTTTGGAATTGGGGGAGGTATCAAGGATGGAAAGAAGTTTAAGGCTGTGCAAACCGGTGGCCCGTCAGGA
GGTTGCTTGACTGAAAAGCATCTTGATCTTCCTATCGATTATGATAATTTGCTTGCCGCTGGTTCCATGATGGGTTCCGG
AGGTATGATCGTCATGGACGAGGATGATTGTATGGTAGCCATGGCTAAGTTCTATCTGGACTTTACGGTCGAGGAATCTT
GCGGTAAATGCGCACCGTGCCGTATCGGTAATAAACGCTTACATGAGATATTGCAGAAAATTACGAGTGGAAACGGAGCC
GAAGAGGATTTGACCCGTCTAAGGAATCTGGCGATGGTGATTAAGGATACCGCTTTATGCGGCTTGGGACAGACTTCTCC
GAATCCGGTTTTATCTACGATGGATAATTTCTACGATGAATATCTGGCGCATGTCCGGGAGCATCGTTGTCCGTCTCATC
AATGCCGGGAACTTACTCAATATTTCATCAATCCGGAGAAATGTAAAGGATGTACGCTCTGTGCCCGTGTTTGTCCGGTG
CATGCGATTACAGGTGGACGGAAAATTCCTCATATTATCGATCCGCAAGCTTGTATCCGTTGCGGTACCTGTATGGAGAA
GTGTAAGTTCGGAGCGATTTATGTCCACTAA

Upstream 100 bases:

>100_bases
CATAGATAAGTATATCCGTAAGGGTATCTTGGTAGACGGCATCATTCCTGTAACTTACACCACCGTAAAACCCGAATAAG
AAATTTAATATCTTACATAA

Downstream 100 bases:

>100_bases
ATTTATTTATAAGCATGGAAACAATTCAATTAATCATAGATAATAAAGAGGTGGAAGTGCCGAGAGGAACCACCATTTTA
GATGCGGCAAAAAGTGTAGG

Product: NADH:ubiquinone oxidoreductase subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MAQYTNYILVCGGTGCRASQSEQIIENLRAAVEQYGLEDTQVIRTGCFGFCEKGPVVKMIPDNTFYVQVQPSDADEIVRE
HLVKGRKVERLLYMNPETRELVPDSKHIRFYKKQLRIALRNCGFINPENIDEYIARDGYVALGKALVEMTPEEVIKEIMD
SGLRGRGGGGFPTGLKWQITRKVQAPQKYVVCNADEGDPGAFMDRSILEGDPHSIVEAMAINGYCTGASKGLVYIRAEYP
LAVERLKIAIRQAKEYGLLGENIFGTDFSFDIEIRYGAGAFVCGEETALIHSMEGLRGEATVKPPFPSEHGYKGCPTNVN
NVETYANIPVILLRGAKWFSSIGTEKSKGTKVFALAGKVNNVGLIEVPMGTTLREVIFGIGGGIKDGKKFKAVQTGGPSG
GCLTEKHLDLPIDYDNLLAAGSMMGSGGMIVMDEDDCMVAMAKFYLDFTVEESCGKCAPCRIGNKRLHEILQKITSGNGA
EEDLTRLRNLAMVIKDTALCGLGQTSPNPVLSTMDNFYDEYLAHVREHRCPSHQCRELTQYFINPEKCKGCTLCARVCPV
HAITGGRKIPHIIDPQACIRCGTCMEKCKFGAIYVH

Sequences:

>Translated_596_residues
MAQYTNYILVCGGTGCRASQSEQIIENLRAAVEQYGLEDTQVIRTGCFGFCEKGPVVKMIPDNTFYVQVQPSDADEIVRE
HLVKGRKVERLLYMNPETRELVPDSKHIRFYKKQLRIALRNCGFINPENIDEYIARDGYVALGKALVEMTPEEVIKEIMD
SGLRGRGGGGFPTGLKWQITRKVQAPQKYVVCNADEGDPGAFMDRSILEGDPHSIVEAMAINGYCTGASKGLVYIRAEYP
LAVERLKIAIRQAKEYGLLGENIFGTDFSFDIEIRYGAGAFVCGEETALIHSMEGLRGEATVKPPFPSEHGYKGCPTNVN
NVETYANIPVILLRGAKWFSSIGTEKSKGTKVFALAGKVNNVGLIEVPMGTTLREVIFGIGGGIKDGKKFKAVQTGGPSG
GCLTEKHLDLPIDYDNLLAAGSMMGSGGMIVMDEDDCMVAMAKFYLDFTVEESCGKCAPCRIGNKRLHEILQKITSGNGA
EEDLTRLRNLAMVIKDTALCGLGQTSPNPVLSTMDNFYDEYLAHVREHRCPSHQCRELTQYFINPEKCKGCTLCARVCPV
HAITGGRKIPHIIDPQACIRCGTCMEKCKFGAIYVH
>Mature_595_residues
AQYTNYILVCGGTGCRASQSEQIIENLRAAVEQYGLEDTQVIRTGCFGFCEKGPVVKMIPDNTFYVQVQPSDADEIVREH
LVKGRKVERLLYMNPETRELVPDSKHIRFYKKQLRIALRNCGFINPENIDEYIARDGYVALGKALVEMTPEEVIKEIMDS
GLRGRGGGGFPTGLKWQITRKVQAPQKYVVCNADEGDPGAFMDRSILEGDPHSIVEAMAINGYCTGASKGLVYIRAEYPL
AVERLKIAIRQAKEYGLLGENIFGTDFSFDIEIRYGAGAFVCGEETALIHSMEGLRGEATVKPPFPSEHGYKGCPTNVNN
VETYANIPVILLRGAKWFSSIGTEKSKGTKVFALAGKVNNVGLIEVPMGTTLREVIFGIGGGIKDGKKFKAVQTGGPSGG
CLTEKHLDLPIDYDNLLAAGSMMGSGGMIVMDEDDCMVAMAKFYLDFTVEESCGKCAPCRIGNKRLHEILQKITSGNGAE
EDLTRLRNLAMVIKDTALCGLGQTSPNPVLSTMDNFYDEYLAHVREHRCPSHQCRELTQYFINPEKCKGCTLCARVCPVH
AITGGRKIPHIIDPQACIRCGTCMEKCKFGAIYVH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=380, Percent_Identity=39.2105263157895, Blast_Score=278, Evalue=2e-74,
Organism=Homo sapiens, GI20149568, Length=380, Percent_Identity=39.2105263157895, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1788620, Length=410, Percent_Identity=40.7317073170732, Blast_Score=333, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17531727, Length=381, Percent_Identity=38.8451443569554, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=40.7407407407407, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI19920794, Length=389, Percent_Identity=38.8174807197943, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24653819, Length=355, Percent_Identity=35.7746478873239, Blast_Score=218, Evalue=9e-57,
Organism=Drosophila melanogaster, GI24653817, Length=325, Percent_Identity=33.5384615384615, Blast_Score=182, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 65528; Mature: 65397

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQYTNYILVCGGTGCRASQSEQIIENLRAAVEQYGLEDTQVIRTGCFGFCEKGPVVKMI
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEC
PDNTFYVQVQPSDADEIVREHLVKGRKVERLLYMNPETRELVPDSKHIRFYKKQLRIALR
CCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHH
NCGFINPENIDEYIARDGYVALGKALVEMTPEEVIKEIMDSGLRGRGGGGFPTGLKWQIT
HCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
RKVQAPQKYVVCNADEGDPGAFMDRSILEGDPHSIVEAMAINGYCTGASKGLVYIRAEYP
EECCCCCEEEEEECCCCCCCCHHCCHHHCCCHHHHHHHHHCCCEEECCCCCEEEEEECCC
LAVERLKIAIRQAKEYGLLGENIFGTDFSFDIEIRYGAGAFVCGEETALIHSMEGLRGEA
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHHHHCCCCCCC
TVKPPFPSEHGYKGCPTNVNNVETYANIPVILLRGAKWFSSIGTEKSKGTKVFALAGKVN
EECCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCHHHHHCCCCCCCCCEEEEEECCCC
NVGLIEVPMGTTLREVIFGIGGGIKDGKKFKAVQTGGPSGGCLTEKHLDLPIDYDNLLAA
CCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
GSMMGSGGMIVMDEDDCMVAMAKFYLDFTVEESCGKCAPCRIGNKRLHEILQKITSGNGA
CCEECCCCEEEEECCCHHHHHHHHHHEEEEHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
EEDLTRLRNLAMVIKDTALCGLGQTSPNPVLSTMDNFYDEYLAHVREHRCPSHQCRELTQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YFINPEKCKGCTLCARVCPVHAITGGRKIPHIIDPQACIRCGTCMEKCKFGAIYVH
HCCCHHHCCCHHHHHHHCCHHEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure 
AQYTNYILVCGGTGCRASQSEQIIENLRAAVEQYGLEDTQVIRTGCFGFCEKGPVVKMI
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEC
PDNTFYVQVQPSDADEIVREHLVKGRKVERLLYMNPETRELVPDSKHIRFYKKQLRIALR
CCCEEEEEECCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHH
NCGFINPENIDEYIARDGYVALGKALVEMTPEEVIKEIMDSGLRGRGGGGFPTGLKWQIT
HCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
RKVQAPQKYVVCNADEGDPGAFMDRSILEGDPHSIVEAMAINGYCTGASKGLVYIRAEYP
EECCCCCEEEEEECCCCCCCCHHCCHHHCCCHHHHHHHHHCCCEEECCCCCEEEEEECCC
LAVERLKIAIRQAKEYGLLGENIFGTDFSFDIEIRYGAGAFVCGEETALIHSMEGLRGEA
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCEEEECCCHHHHHHHCCCCCCC
TVKPPFPSEHGYKGCPTNVNNVETYANIPVILLRGAKWFSSIGTEKSKGTKVFALAGKVN
EECCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCHHHHHCCCCCCCCCEEEEEECCCC
NVGLIEVPMGTTLREVIFGIGGGIKDGKKFKAVQTGGPSGGCLTEKHLDLPIDYDNLLAA
CCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
GSMMGSGGMIVMDEDDCMVAMAKFYLDFTVEESCGKCAPCRIGNKRLHEILQKITSGNGA
CCEECCCCEEEEECCCHHHHHHHHHHEEEEHHCCCCCCCCCCCCHHHHHHHHHHHCCCCC
EEDLTRLRNLAMVIKDTALCGLGQTSPNPVLSTMDNFYDEYLAHVREHRCPSHQCRELTQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YFINPEKCKGCTLCARVCPVHAITGGRKIPHIIDPQACIRCGTCMEKCKFGAIYVH
HCCCHHHCCCHHHHHHHCCHHEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]