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The map label for this gene is phoA [H]

Identifier: 150007559

GI number: 150007559

Start: 1046088

End: 1047788

Strand: Reverse

Name: phoA [H]

Synonym: BDI_0912

Alternate gene names: 150007559

Gene position: 1047788-1046088 (Counterclockwise)

Preceding gene: 150007560

Following gene: 150007558

Centisome position: 21.78

GC content: 48.21

Gene sequence:

>1701_bases
ATGAAAAAAACATTAGCTTTTCTGCTTTTGCTGGCTATTATCATCATGCCGGCCAATGCCGTGGAAAAGGAGAACGTGAA
GCCTGTCAAGAACGTGATCCTTTTAATCCCGGACGGAACCTCATTGGCGACTATCTCTATCGCCCGTTGGTTGCAATGGT
ACCAAGATCCCAGCAAACCGAAATTGAACATCGACCCGTATTTGTGCGGAACGGTGCGTACGCATTCTTCTAACGCCCCT
ATCGGCGACTCCGCTCCTACTACATCATGCTACATGACCGGACAACCGAGCCGTACGGGTTATGTATCCACTTACCCGGA
GAACGACGGTGATAACGATATCTACCCGACCGACCCGGCCCGTGCCTTCCAACCGCTTACCACTGTATTGGAGGCTGGAA
AGATGTTGCAGGGCAAAGCCACGGGATTGGTTTTCACTTGCGAATTCCCGCACGCTACCCCGGCGGACTGCTCCGCTCAT
AGCTACAACCGTGGTAAATACGACTGGATCGCTCCGCAGATGGTTCATAATGATATCGATGTCGTTATCGGTGGTGGAGT
TTCTATCCTTACAAAGGATATGGAAGATTATCTATTGGCGAACGGATACGGCGTATATAGAAACGACCTGAAAGGTATGC
GTGCCGATAATAATCAGAAGATGTGGGCTTTATATGGCAACAAGGAAATGGCTTACGATATCGACCGCAATCCGGAAGAG
CAACCTTCTATCGAGGAAATGACTCGTAAGGCGATCGACAAATTATCCAAGAACCCGAATGGCTTCTTCTTAATGGTAGA
AGGCAGTAAGGTAGACTGGGCCGCTCATGGAAATGACCCGGTAGGTATGGCTACCGATTTCTTGGCATTCGATCGTGCCT
GCGGTGCCGCATTGGAGTTCGCACGCAATAACGGCGAGACTGCCGTTGTCATCGTACCCGACCACGGAAACAGCGGTATC
AGCTTAGGTAGAAGAGATTGTAAAGGTTACGATAAATTGACGAAAGATCAATTGTTCCACCAATTCTCCCTGTATAAATT
GACCGCCGAGGGTTTCGCCAACAAGGTAAACAGCGTTCCTAACTCAGAAGTGCAAAACGTATTCCGCACCTACGCCGGCT
TCGAGCTGACACCGGAAGAGATGAATGCGTTGAACAATTGCAAGGATTATAAGAATAGCCCGATACCGGAAGACCAACGC
AAACCAGAAGATAATTCCTCCATGTACAGTGGCTCGTTAACCGGATTGATGGCGCATATCATCACTTCCCGCACTTGCTT
CGGTTTCACGACCGGCGGCCATACGGGCGAGGAAGTATTCCTTGCCGCTTACCATCCGCAAGGAACCTTACCCCTAGGTA
TGAATACGAACATCGAGCTGAATGAGTACTTATGCAATTTGTTCGGATTGACACACGAAAACTTGGAAGATCTTACAAAC
AAGAACTTCGCACGCCACACGGACGTATTCGAGGATTACACTTGCGAGATCGTCCCTGCCGCCGACGAGAAAGGTTCTCC
AACCTTGATCGTTAAGAACAAGAAAGATAAGAAAAAACAGCTAACGATCACCCCGTTCTCCAATATCGTGAAGTCCGGGA
AGAAAGGCCAAGATGAGATACGGTTAAACTCTGTCGTCGTTTATGTCGATAAAAATAACACGTTCTATCTTCCGGCAAGT
CTAGTTGATTTCTTAAAATAA

Upstream 100 bases:

>100_bases
ACGGGCGTGGAGAAGTTGACACGGTTCAACGAGGAGATCATCCCACTTGGAAATTAGGATTTAATAGAAGAATTTGTTAT
ACATTATTAAATTAAAGCAC

Downstream 100 bases:

>100_bases
AATATAAAGCGGAGGCCCTTCGGTCTCCGCTTTTTTTATACATTCGCGTACATAAGACAGAATATACACAACCTATGAAA
AGTAAAAGTTATTGTTCAAT

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKKTLAFLLLLAIIIMPANAVEKENVKPVKNVILLIPDGTSLATISIARWLQWYQDPSKPKLNIDPYLCGTVRTHSSNAP
IGDSAPTTSCYMTGQPSRTGYVSTYPENDGDNDIYPTDPARAFQPLTTVLEAGKMLQGKATGLVFTCEFPHATPADCSAH
SYNRGKYDWIAPQMVHNDIDVVIGGGVSILTKDMEDYLLANGYGVYRNDLKGMRADNNQKMWALYGNKEMAYDIDRNPEE
QPSIEEMTRKAIDKLSKNPNGFFLMVEGSKVDWAAHGNDPVGMATDFLAFDRACGAALEFARNNGETAVVIVPDHGNSGI
SLGRRDCKGYDKLTKDQLFHQFSLYKLTAEGFANKVNSVPNSEVQNVFRTYAGFELTPEEMNALNNCKDYKNSPIPEDQR
KPEDNSSMYSGSLTGLMAHIITSRTCFGFTTGGHTGEEVFLAAYHPQGTLPLGMNTNIELNEYLCNLFGLTHENLEDLTN
KNFARHTDVFEDYTCEIVPAADEKGSPTLIVKNKKDKKKQLTITPFSNIVKSGKKGQDEIRLNSVVVYVDKNNTFYLPAS
LVDFLK

Sequences:

>Translated_566_residues
MKKTLAFLLLLAIIIMPANAVEKENVKPVKNVILLIPDGTSLATISIARWLQWYQDPSKPKLNIDPYLCGTVRTHSSNAP
IGDSAPTTSCYMTGQPSRTGYVSTYPENDGDNDIYPTDPARAFQPLTTVLEAGKMLQGKATGLVFTCEFPHATPADCSAH
SYNRGKYDWIAPQMVHNDIDVVIGGGVSILTKDMEDYLLANGYGVYRNDLKGMRADNNQKMWALYGNKEMAYDIDRNPEE
QPSIEEMTRKAIDKLSKNPNGFFLMVEGSKVDWAAHGNDPVGMATDFLAFDRACGAALEFARNNGETAVVIVPDHGNSGI
SLGRRDCKGYDKLTKDQLFHQFSLYKLTAEGFANKVNSVPNSEVQNVFRTYAGFELTPEEMNALNNCKDYKNSPIPEDQR
KPEDNSSMYSGSLTGLMAHIITSRTCFGFTTGGHTGEEVFLAAYHPQGTLPLGMNTNIELNEYLCNLFGLTHENLEDLTN
KNFARHTDVFEDYTCEIVPAADEKGSPTLIVKNKKDKKKQLTITPFSNIVKSGKKGQDEIRLNSVVVYVDKNNTFYLPAS
LVDFLK
>Mature_566_residues
MKKTLAFLLLLAIIIMPANAVEKENVKPVKNVILLIPDGTSLATISIARWLQWYQDPSKPKLNIDPYLCGTVRTHSSNAP
IGDSAPTTSCYMTGQPSRTGYVSTYPENDGDNDIYPTDPARAFQPLTTVLEAGKMLQGKATGLVFTCEFPHATPADCSAH
SYNRGKYDWIAPQMVHNDIDVVIGGGVSILTKDMEDYLLANGYGVYRNDLKGMRADNNQKMWALYGNKEMAYDIDRNPEE
QPSIEEMTRKAIDKLSKNPNGFFLMVEGSKVDWAAHGNDPVGMATDFLAFDRACGAALEFARNNGETAVVIVPDHGNSGI
SLGRRDCKGYDKLTKDQLFHQFSLYKLTAEGFANKVNSVPNSEVQNVFRTYAGFELTPEEMNALNNCKDYKNSPIPEDQR
KPEDNSSMYSGSLTGLMAHIITSRTCFGFTTGGHTGEEVFLAAYHPQGTLPLGMNTNIELNEYLCNLFGLTHENLEDLTN
KNFARHTDVFEDYTCEIVPAADEKGSPTLIVKNKKDKKKQLTITPFSNIVKSGKKGQDEIRLNSVVVYVDKNNTFYLPAS
LVDFLK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=351, Percent_Identity=28.7749287749288, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI157266292, Length=341, Percent_Identity=27.8592375366569, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI94721246, Length=336, Percent_Identity=26.7857142857143, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI157266296, Length=338, Percent_Identity=26.9230769230769, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI48994877, Length=352, Percent_Identity=28.9772727272727, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320689, Length=537, Percent_Identity=26.6294227188082, Blast_Score=147, Evalue=5e-36,
Organism=Drosophila melanogaster, GI21355151, Length=340, Percent_Identity=26.4705882352941, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI18859923, Length=316, Percent_Identity=26.5822784810127, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355981, Length=335, Percent_Identity=26.5671641791045, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24657827, Length=339, Percent_Identity=28.023598820059, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24657835, Length=342, Percent_Identity=26.6081871345029, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19921912, Length=371, Percent_Identity=26.1455525606469, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI85725200, Length=345, Percent_Identity=26.0869565217391, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI85815833, Length=345, Percent_Identity=26.0869565217391, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21358067, Length=335, Percent_Identity=26.2686567164179, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355149, Length=355, Percent_Identity=27.6056338028169, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24657842, Length=346, Percent_Identity=25.7225433526012, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24585213, Length=366, Percent_Identity=25.4098360655738, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 62741; Mature: 62741

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLAFLLLLAIIIMPANAVEKENVKPVKNVILLIPDGTSLATISIARWLQWYQDPSKP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
KLNIDPYLCGTVRTHSSNAPIGDSAPTTSCYMTGQPSRTGYVSTYPENDGDNDIYPTDPA
CCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCCHH
RAFQPLTTVLEAGKMLQGKATGLVFTCEFPHATPADCSAHSYNRGKYDWIAPQMVHNDID
HHHHHHHHHHHHCHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECHHHHCCCCE
VVIGGGVSILTKDMEDYLLANGYGVYRNDLKGMRADNNQKMWALYGNKEMAYDIDRNPEE
EEECCCHHEEHHHHHHHHHHCCCCEEHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCC
QPSIEEMTRKAIDKLSKNPNGFFLMVEGSKVDWAAHGNDPVGMATDFLAFDRACGAALEF
CCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ARNNGETAVVIVPDHGNSGISLGRRDCKGYDKLTKDQLFHQFSLYKLTAEGFANKVNSVP
HHCCCCEEEEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHCCCC
NSEVQNVFRTYAGFELTPEEMNALNNCKDYKNSPIPEDQRKPEDNSSMYSGSLTGLMAHI
CHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEECCHHHHHHHHH
ITSRTCFGFTTGGHTGEEVFLAAYHPQGTLPLGMNTNIELNEYLCNLFGLTHENLEDLTN
HHCCCEEEEECCCCCCCEEEEEEECCCCCEEECCCCCEEHHHHHHHHHCCCHHHHHHHHC
KNFARHTDVFEDYTCEIVPAADEKGSPTLIVKNKKDKKKQLTITPFSNIVKSGKKGQDEI
CCCHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCCCCCCEE
RLNSVVVYVDKNNTFYLPASLVDFLK
EEEEEEEEEECCCEEEECHHHHHHHC
>Mature Secondary Structure
MKKTLAFLLLLAIIIMPANAVEKENVKPVKNVILLIPDGTSLATISIARWLQWYQDPSKP
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCC
KLNIDPYLCGTVRTHSSNAPIGDSAPTTSCYMTGQPSRTGYVSTYPENDGDNDIYPTDPA
CCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCCCCCCCCCCHH
RAFQPLTTVLEAGKMLQGKATGLVFTCEFPHATPADCSAHSYNRGKYDWIAPQMVHNDID
HHHHHHHHHHHHCHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECHHHHCCCCE
VVIGGGVSILTKDMEDYLLANGYGVYRNDLKGMRADNNQKMWALYGNKEMAYDIDRNPEE
EEECCCHHEEHHHHHHHHHHCCCCEEHHCCCCCCCCCCCEEEEEECCCEEEEECCCCCCC
QPSIEEMTRKAIDKLSKNPNGFFLMVEGSKVDWAAHGNDPVGMATDFLAFDRACGAALEF
CCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
ARNNGETAVVIVPDHGNSGISLGRRDCKGYDKLTKDQLFHQFSLYKLTAEGFANKVNSVP
HHCCCCEEEEEEECCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHEEEEHHHHHHHHCCCC
NSEVQNVFRTYAGFELTPEEMNALNNCKDYKNSPIPEDQRKPEDNSSMYSGSLTGLMAHI
CHHHHHHHHHHCCCEECHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCEECCHHHHHHHHH
ITSRTCFGFTTGGHTGEEVFLAAYHPQGTLPLGMNTNIELNEYLCNLFGLTHENLEDLTN
HHCCCEEEEECCCCCCCEEEEEEECCCCCEEECCCCCEEHHHHHHHHHCCCHHHHHHHHC
KNFARHTDVFEDYTCEIVPAADEKGSPTLIVKNKKDKKKQLTITPFSNIVKSGKKGQDEI
CCCHHHCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCCCCCCEE
RLNSVVVYVDKNNTFYLPASLVDFLK
EEEEEEEEEECCCEEEECHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]