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The map label for this gene is gadB [H]

Identifier: 150007470

GI number: 150007470

Start: 968791

End: 970230

Strand: Reverse

Name: gadB [H]

Synonym: BDI_0821

Alternate gene names: 150007470

Gene position: 970230-968791 (Counterclockwise)

Preceding gene: 150007473

Following gene: 150007469

Centisome position: 20.17

GC content: 44.38

Gene sequence:

>1440_bases
ATGAAAGACTCAAATTTCAGAAATGGCGATGCCAAAACAGCGATTTTTGGCTCAGAAGTGATGTTGACACCCTCTCCTGT
AGATAAGATTCCAGATGGCCCTACAACCCCGGAGATCGCTTATCAAATGGTTAAAGACGAGACTTTCGCACAAACACAGC
CTCGCTTGAACCTAGCTACATTCGTTACTACGTATATGGACGAGTACGCGACAAAATTGATGAACGAGGCTATCAACATC
AACTATATTGACGAAACAGAGTATCCGAGAATCGCTGTTATGAACGCCAAATGTATTAATATCATGGCGAGTCTTTGGAA
TTCTCCCGAGCAAGAAAAATGGAAAACCGGAGCCTTGGCTATCGGTTCTTCAGAGGCTTGTATGCTAGGTGGTGTAGCCG
CTTGGTTACGTTGGCGCAAAAAGAGACAAGCACAAGGTAAACCAACTGACAAGCCTAATTTCGTGATCTCTACTGGCTTC
CAAGTCGTATGGGAGAAATTCGCCCAGCTATGGCAAATCGAAATGCGTGAAGTTCCCTTGACTTTAGACAAGACGACACT
TGATCCGGAAGAAGCACTTAAGATGTGTGACGAGAACACGATTTGTATCGTTCCTATCCAAGGTGTAACATGGACAGGAC
TGAACGATGACGTAGAAGCCTTGGACAAAGCGCTCGACGCATACAATGCCAAGACCGGTTATGATATACCTATCCACGTA
GATGCCGCCAGCGGTGGTTTTATCCTTCCGTTCTTAAGCCCGGAGACAAAATGGGACTTCCGTTTGAAATGGGTTCTTTC
TATCAGCACATCCGGACATAAATTCGGATTGGTTTACCCGGGATTGGGTTGGGTTGTTTGGAAAGACAAGAAATATTTGC
CGGAAGAAATGGCATTCAGCGTAAACTATCTTGGAGCGAATATCACTCAGGTTGGTTTGAACTTCTCTCGTCCTGCCGCA
CAAATCCTCGGACAATACTATCAGTTTATCCGTCTCGGATTCGAGGGCTACAAACAAATCCAATATAACAGTATGGAAAT
CACGAAATACATCCACCAAGAAATCGGTAAGATGGCCCCATTCGTTAATTACAGCAACGAGGTTGTGAATCCATTGTTTA
TTTGGTACATGAAACCGGATTACGCTAAGAACGCCAAATGGACACTTTACGATTTGCAAGATAAATTGCAACAAAGCGGT
TGGATGGTTCCAGCGTATACACTACCGAAGAAGCTGGATAACTACGTGGTTATGCGTGTCGTAGTTCGTCAAGGTTTCAG
CCGTGATATGGCCGATATGTTATTAGGTGATATCAAGAACGCTGTAGAGGCATTGGAGAAACTTGAATATCCGACTCCTA
CTCGCATCGCTCAAGACAAAAACGTTCCGGTTAAGGGTAAGGTATTTACTCACACCGCAATGAGCAACGCTAAGAAATAA

Upstream 100 bases:

>100_bases
AAAAAACTTTCCAATCCATAATCTGTTTTGGCATAAACGCTCAGTGGAAGCAGTATTCCATTGATTCAAATCATTTTATT
AACAAAAAAACTAAAGAAAA

Downstream 100 bases:

>100_bases
AAACAAAATTCATCAAGGCGTGCATGAAAGGACTATCAGTGTTTTCATACACGCCTTCTTTAATCCAATTCATATATGGA
CAAAAAAATAACGATCGCCC

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKDSNFRNGDAKTAIFGSEVMLTPSPVDKIPDGPTTPEIAYQMVKDETFAQTQPRLNLATFVTTYMDEYATKLMNEAINI
NYIDETEYPRIAVMNAKCINIMASLWNSPEQEKWKTGALAIGSSEACMLGGVAAWLRWRKKRQAQGKPTDKPNFVISTGF
QVVWEKFAQLWQIEMREVPLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPIHV
DAASGGFILPFLSPETKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPEEMAFSVNYLGANITQVGLNFSRPAA
QILGQYYQFIRLGFEGYKQIQYNSMEITKYIHQEIGKMAPFVNYSNEVVNPLFIWYMKPDYAKNAKWTLYDLQDKLQQSG
WMVPAYTLPKKLDNYVVMRVVVRQGFSRDMADMLLGDIKNAVEALEKLEYPTPTRIAQDKNVPVKGKVFTHTAMSNAKK

Sequences:

>Translated_479_residues
MKDSNFRNGDAKTAIFGSEVMLTPSPVDKIPDGPTTPEIAYQMVKDETFAQTQPRLNLATFVTTYMDEYATKLMNEAINI
NYIDETEYPRIAVMNAKCINIMASLWNSPEQEKWKTGALAIGSSEACMLGGVAAWLRWRKKRQAQGKPTDKPNFVISTGF
QVVWEKFAQLWQIEMREVPLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPIHV
DAASGGFILPFLSPETKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPEEMAFSVNYLGANITQVGLNFSRPAA
QILGQYYQFIRLGFEGYKQIQYNSMEITKYIHQEIGKMAPFVNYSNEVVNPLFIWYMKPDYAKNAKWTLYDLQDKLQQSG
WMVPAYTLPKKLDNYVVMRVVVRQGFSRDMADMLLGDIKNAVEALEKLEYPTPTRIAQDKNVPVKGKVFTHTAMSNAKK
>Mature_479_residues
MKDSNFRNGDAKTAIFGSEVMLTPSPVDKIPDGPTTPEIAYQMVKDETFAQTQPRLNLATFVTTYMDEYATKLMNEAINI
NYIDETEYPRIAVMNAKCINIMASLWNSPEQEKWKTGALAIGSSEACMLGGVAAWLRWRKKRQAQGKPTDKPNFVISTGF
QVVWEKFAQLWQIEMREVPLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPIHV
DAASGGFILPFLSPETKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPEEMAFSVNYLGANITQVGLNFSRPAA
QILGQYYQFIRLGFEGYKQIQYNSMEITKYIHQEIGKMAPFVNYSNEVVNPLFIWYMKPDYAKNAKWTLYDLQDKLQQSG
WMVPAYTLPKKLDNYVVMRVVVRQGFSRDMADMLLGDIKNAVEALEKLEYPTPTRIAQDKNVPVKGKVFTHTAMSNAKK

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=365, Percent_Identity=21.6438356164384, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789934, Length=462, Percent_Identity=43.0735930735931, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787769, Length=462, Percent_Identity=43.0735930735931, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17557272, Length=363, Percent_Identity=23.1404958677686, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17543922, Length=321, Percent_Identity=24.2990654205607, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25148342, Length=368, Percent_Identity=21.195652173913, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323905, Length=463, Percent_Identity=34.1252699784017, Blast_Score=269, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6320500, Length=341, Percent_Identity=21.7008797653959, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355963, Length=409, Percent_Identity=22.0048899755501, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24654344, Length=409, Percent_Identity=22.0048899755501, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 3820 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 54452; Mature: 54452

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSNFRNGDAKTAIFGSEVMLTPSPVDKIPDGPTTPEIAYQMVKDETFAQTQPRLNLAT
CCCCCCCCCCCCEEEECCEEEECCCCHHCCCCCCCCHHHHHHHHHCCHHHHCCCCCHHHH
FVTTYMDEYATKLMNEAININYIDETEYPRIAVMNAKCINIMASLWNSPEQEKWKTGALA
HHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECHHHHHHHHHHHCCCCHHHHCCCEEE
IGSSEACMLGGVAAWLRWRKKRQAQGKPTDKPNFVISTGFQVVWEKFAQLWQIEMREVPL
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCE
TLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPIHV
EECCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEE
DAASGGFILPFLSPETKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPEEMAFS
ECCCCCEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCHHHHEE
VNYLGANITQVGLNFSRPAAQILGQYYQFIRLGFEGYKQIQYNSMEITKYIHQEIGKMAP
EHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCC
FVNYSNEVVNPLFIWYMKPDYAKNAKWTLYDLQDKLQQSGWMVPAYTLPKKLDNYVVMRV
CCCCCCHHCCEEEEEEECCCCCCCCCEEEEHHHHHHHHCCCEEEEHHCHHHHHHHHHHHH
VVRQGFSRDMADMLLGDIKNAVEALEKLEYPTPTRIAQDKNVPVKGKVFTHTAMSNAKK
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCC
>Mature Secondary Structure
MKDSNFRNGDAKTAIFGSEVMLTPSPVDKIPDGPTTPEIAYQMVKDETFAQTQPRLNLAT
CCCCCCCCCCCCEEEECCEEEECCCCHHCCCCCCCCHHHHHHHHHCCHHHHCCCCCHHHH
FVTTYMDEYATKLMNEAININYIDETEYPRIAVMNAKCINIMASLWNSPEQEKWKTGALA
HHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEECHHHHHHHHHHHCCCCHHHHCCCEEE
IGSSEACMLGGVAAWLRWRKKRQAQGKPTDKPNFVISTGFQVVWEKFAQLWQIEMREVPL
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCE
TLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPIHV
EECCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEE
DAASGGFILPFLSPETKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPEEMAFS
ECCCCCEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCHHHHEE
VNYLGANITQVGLNFSRPAAQILGQYYQFIRLGFEGYKQIQYNSMEITKYIHQEIGKMAP
EHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCC
FVNYSNEVVNPLFIWYMKPDYAKNAKWTLYDLQDKLQQSGWMVPAYTLPKKLDNYVVMRV
CCCCCCHHCCEEEEEEECCCCCCCCCEEEEHHHHHHHHCCCEEEEHHCHHHHHHHHHHHH
VVRQGFSRDMADMLLGDIKNAVEALEKLEYPTPTRIAQDKNVPVKGKVFTHTAMSNAKK
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10411264; 11337471 [H]