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The map label for this gene is folC [H]

Identifier: 150007410

GI number: 150007410

Start: 889614

End: 890933

Strand: Reverse

Name: folC [H]

Synonym: BDI_0761

Alternate gene names: 150007410

Gene position: 890933-889614 (Counterclockwise)

Preceding gene: 150007412

Following gene: 150007409

Centisome position: 18.52

GC content: 50.15

Gene sequence:

>1320_bases
ATGACATACGAGGAAACGCTACACTATCTATATACCAGCATCCCGGTCTTCCAACACAGCGGAGCTTCCGCTTACAAACC
CGGTCTTGGTACAAGTATCGCCCTAGATGATTATCTGGGAAATCCGCATAAAGCCTATAAGACCATCCATGTAGCCGGGA
CAAACGGCAAAGGCTCGGTCAGCCATCTATTGGCGGCCATTCTCCGGCAATCCGGTTTCAAGGTCGGGCTTTATACCTCT
CCCCACTTAGTGGACTTCCGGGAACGCATCCGGGTGAACGGGAAAAAGATCTCCCAAGATTACGTGGTTGATTTCGTCGA
GCGTCATCGTTCTTTCTTCGAGCCTCTCCATCCTTCTTTCTTCGAGTTAACGTCCAGCATGGCTTTTGAGTATTTCCGCG
ACCAGCAGGTAAATTACGCGGTTATCGAGGTCGGGCTTGGCGGTCGTTTGGACAGTACGAACATTATCACTCCGATCTTG
AGTATTATCACGAATATCAGTCTGGATCATACGCAATTCTTGGGAGATACGGAGGAGAAGATAGCGGCGGAGAAAGCCGG
CATTATCAAGAAAGGCGTACCGGCACTTCTTGGGGAAGCCGAGAAACAATCGGTTTACGACGTTTTCAAAAACAAGGCGG
AAGAAGTTGGCGCACCTTTCTACTACGCCCAACGAATGGACATGGTAAAAGACGAGTGCAGGCTGACGGTCGGTGGCAAA
TGGTTCTTCCCTTCCGTTGAATATGGAGAAATCCAAGGAGAACTTGGCGGTGAGGTGCAGGTAAGAAATGCTGTCACCGT
ATTATCCGCCATCCATATATTACGAACGGAATTGGGTGTAGATATCATCCCGGAAGCGGTACACGAGGGTTTCGCCCACG
TAACGAAACTGACGGGATTGATGGGACGTTGGCAAACCTTGCGATCCAAACCGACCGTCATTTGCGACACGGGGCATAAT
GTAGGAGGCTGGGAGCATCTAGCGGAGCATCTGAAAGTCTTGCGTCAATATTGCGGGCAAATCTATATGATCGTCGGCAT
GGTAAACGACAAGGATATCGATGGCGTATTATCCTTGATGCCGCAAGACGCTTTTTATTTCTTCACGCAAGCCTCCGTGG
AGAGAGCGATGCCCGTAGAGGATTTCGCCGTGAAAGCCATGCGTCACGGACTGTCCGGTACCCTTTGCGATACGGTGCAA
GACGCTGTCGAGAAAGCGTTAAGAAGGGCTCGTGAGAACGACCTGATCTTTATCGGAGGCAGTACGTTTATCGTAGCGGA
CGCTCTCCCCTTGTTCATGAAAGAAGACGAATACAAATAA

Upstream 100 bases:

>100_bases
ATACGTCGTGTTTCCATTTTCTTTTTCTCTCCTTTTTATAAACGAGTCGCAAAGGTATAAATAAAATTAACTACATTTGT
AGCATCTAATTTACAAAAAC

Downstream 100 bases:

>100_bases
TATAACTCTATAAAACAAATATCATGATAGAAAAAATTCATCAGAAACTAAGCGACTCGAAAGTCGCACGCTGGAGTGTA
CTCGCTTTGGCGGCATTCAC

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTYEETLHYLYTSIPVFQHSGASAYKPGLGTSIALDDYLGNPHKAYKTIHVAGTNGKGSVSHLLAAILRQSGFKVGLYTS
PHLVDFRERIRVNGKKISQDYVVDFVERHRSFFEPLHPSFFELTSSMAFEYFRDQQVNYAVIEVGLGGRLDSTNIITPIL
SIITNISLDHTQFLGDTEEKIAAEKAGIIKKGVPALLGEAEKQSVYDVFKNKAEEVGAPFYYAQRMDMVKDECRLTVGGK
WFFPSVEYGEIQGELGGEVQVRNAVTVLSAIHILRTELGVDIIPEAVHEGFAHVTKLTGLMGRWQTLRSKPTVICDTGHN
VGGWEHLAEHLKVLRQYCGQIYMIVGMVNDKDIDGVLSLMPQDAFYFFTQASVERAMPVEDFAVKAMRHGLSGTLCDTVQ
DAVEKALRRARENDLIFIGGSTFIVADALPLFMKEDEYK

Sequences:

>Translated_439_residues
MTYEETLHYLYTSIPVFQHSGASAYKPGLGTSIALDDYLGNPHKAYKTIHVAGTNGKGSVSHLLAAILRQSGFKVGLYTS
PHLVDFRERIRVNGKKISQDYVVDFVERHRSFFEPLHPSFFELTSSMAFEYFRDQQVNYAVIEVGLGGRLDSTNIITPIL
SIITNISLDHTQFLGDTEEKIAAEKAGIIKKGVPALLGEAEKQSVYDVFKNKAEEVGAPFYYAQRMDMVKDECRLTVGGK
WFFPSVEYGEIQGELGGEVQVRNAVTVLSAIHILRTELGVDIIPEAVHEGFAHVTKLTGLMGRWQTLRSKPTVICDTGHN
VGGWEHLAEHLKVLRQYCGQIYMIVGMVNDKDIDGVLSLMPQDAFYFFTQASVERAMPVEDFAVKAMRHGLSGTLCDTVQ
DAVEKALRRARENDLIFIGGSTFIVADALPLFMKEDEYK
>Mature_438_residues
TYEETLHYLYTSIPVFQHSGASAYKPGLGTSIALDDYLGNPHKAYKTIHVAGTNGKGSVSHLLAAILRQSGFKVGLYTSP
HLVDFRERIRVNGKKISQDYVVDFVERHRSFFEPLHPSFFELTSSMAFEYFRDQQVNYAVIEVGLGGRLDSTNIITPILS
IITNISLDHTQFLGDTEEKIAAEKAGIIKKGVPALLGEAEKQSVYDVFKNKAEEVGAPFYYAQRMDMVKDECRLTVGGKW
FFPSVEYGEIQGELGGEVQVRNAVTVLSAIHILRTELGVDIIPEAVHEGFAHVTKLTGLMGRWQTLRSKPTVICDTGHNV
GGWEHLAEHLKVLRQYCGQIYMIVGMVNDKDIDGVLSLMPQDAFYFFTQASVERAMPVEDFAVKAMRHGLSGTLCDTVQD
AVEKALRRARENDLIFIGGSTFIVADALPLFMKEDEYK

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=233, Percent_Identity=36.9098712446352, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI66932984, Length=233, Percent_Identity=36.9098712446352, Blast_Score=147, Evalue=3e-35,
Organism=Escherichia coli, GI1788654, Length=383, Percent_Identity=33.420365535248, Blast_Score=168, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17553148, Length=181, Percent_Identity=37.0165745856354, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=181, Percent_Identity=37.0165745856354, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=180, Percent_Identity=37.2222222222222, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=378, Percent_Identity=30.6878306878307, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=185, Percent_Identity=38.9189189189189, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322718, Length=200, Percent_Identity=27.5, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641571, Length=183, Percent_Identity=35.5191256830601, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24581568, Length=178, Percent_Identity=32.0224719101124, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48972; Mature: 48840

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYEETLHYLYTSIPVFQHSGASAYKPGLGTSIALDDYLGNPHKAYKTIHVAGTNGKGSV
CCHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEEHHHCCCCCHHEEEEEEECCCCCCHH
SHLLAAILRQSGFKVGLYTSPHLVDFRERIRVNGKKISQDYVVDFVERHRSFFEPLHPSF
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
FELTSSMAFEYFRDQQVNYAVIEVGLGGRLDSTNIITPILSIITNISLDHTQFLGDTEEK
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCHHH
IAAEKAGIIKKGVPALLGEAEKQSVYDVFKNKAEEVGAPFYYAQRMDMVKDECRLTVGGK
HHHHHHCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEECCE
WFFPSVEYGEIQGELGGEVQVRNAVTVLSAIHILRTELGVDIIPEAVHEGFAHVTKLTGL
EECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MGRWQTLRSKPTVICDTGHNVGGWEHLAEHLKVLRQYCGQIYMIVGMVNDKDIDGVLSLM
HHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHC
PQDAFYFFTQASVERAMPVEDFAVKAMRHGLSGTLCDTVQDAVEKALRRARENDLIFIGG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
STFIVADALPLFMKEDEYK
CEEEEHHHHHHHHCCCCCC
>Mature Secondary Structure 
TYEETLHYLYTSIPVFQHSGASAYKPGLGTSIALDDYLGNPHKAYKTIHVAGTNGKGSV
CHHHHHHHHHHHCCHHCCCCCCCCCCCCCCEEEEHHHCCCCCHHEEEEEEECCCCCCHH
SHLLAAILRQSGFKVGLYTSPHLVDFRERIRVNGKKISQDYVVDFVERHRSFFEPLHPSF
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
FELTSSMAFEYFRDQQVNYAVIEVGLGGRLDSTNIITPILSIITNISLDHTQFLGDTEEK
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCCCHHH
IAAEKAGIIKKGVPALLGEAEKQSVYDVFKNKAEEVGAPFYYAQRMDMVKDECRLTVGGK
HHHHHHCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEECCE
WFFPSVEYGEIQGELGGEVQVRNAVTVLSAIHILRTELGVDIIPEAVHEGFAHVTKLTGL
EECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MGRWQTLRSKPTVICDTGHNVGGWEHLAEHLKVLRQYCGQIYMIVGMVNDKDIDGVLSLM
HHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHC
PQDAFYFFTQASVERAMPVEDFAVKAMRHGLSGTLCDTVQDAVEKALRRARENDLIFIGG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
STFIVADALPLFMKEDEYK
CEEEEHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]