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The map label for this gene is rnfC [H]

Identifier: 150007170

GI number: 150007170

Start: 607164

End: 608495

Strand: Reverse

Name: rnfC [H]

Synonym: BDI_0515

Alternate gene names: 150007170

Gene position: 608495-607164 (Counterclockwise)

Preceding gene: 150007171

Following gene: 150007169

Centisome position: 12.65

GC content: 51.5

Gene sequence:

>1332_bases
ATGCTAAGGACATTTCGAATCGGAGGTATACATCCCCCCGAGAATAAACTGTCTGCCGGTAAGAAAATTACGGCTCTCGC
TACGCCTAAACAGGTGATTATCCCCCTCAGCCAGCATATCGGTGCCCCAGCGCAGGCCGTGGTGAAGAAAGGGGATCTGG
TAAAGGTGGGTACATTGCTAGCCAAGGCGGGTGGTTTCGTATCAGCGAACATCCACTCTTCCGTATCCGGCAAAGTGAAC
AAGATAGATAACGCTCTCGACGCTAGCGGATACAAACGTCCCGCTATCTATATAGATGTGGAAGGTGATGAATGGGAAGA
GACGATCGACCGCAGCGAGACCTTAGTGAAGGAGTGCACGCTGTCTTCCAAGGAAATCGTGGATAAGATCGCCGCTTCCG
GTATCGTCGGTCTGGGTGGAGCGACTTTCCCTACGCAGGTGAAATTGATGCCGCCTCCCGGAAACAAGGCAGAGATCGTA
ATCATCAACGCCGTGGAGTGTGAGCCTTATCTTACTTCCGACCATTCGTTGATGCTGGAGAAAGGCGAGCAAATCCTAGT
CGGTGTCACGATCCTAATGAAAGCGGTCAACGTGAACAAGGCCGTGATCGGTATCGAGAATAACAAGCCGGACGCTATCG
CCCATCTGCAAAAGTTGGCGGTAAATTATAAAGGTATAGAGATCATGCCGCTTAAGGTACAATATCCGCAAGGAGGCGAG
AAACAATTGATCGACGCCGTTATTCGCCGTCAGGTAAAAAGTGGTGCGCTTCCTATCTCGGCGGGTGCCGTGGTACAGAA
CGTAGGAACTGCTTATGCCGTTTACGAGGCGGTACAAAAGAATAAACCGTTGTTCGAGCGTGTGGTGACGATCACGGGTA
AGGCGGTAGCTAATCCTTCCAATTTCTTGGTTCGTATGGGAACACCGATCAGTTGCTTGATCGAGGCCGCAGGTGGCCTG
CCCGAGAATACGGGTAAGATTATCGGTGGCGGACCGATGATGGGTAAAGCGTTGATCAGCGCCGAGGTGCCGGTTTGCAA
AGGAAGCTCCGGTGTTTTATTACTGACAACGGAGGAATCTGTCCGCAAGCCGATCCGGGATTGCATCCGTTGCGCCAAAT
GCGTAGGTGTCTGCCCGATGGGATTGAACCCTACCCTATTGATGAACGCCACGGAATTCCAGAATTGGGAATTGGCGGAG
AAGAACTATATCACCGACTGTATCGAGTGCGGGTCGTGTAGTTATACCTGTCCGGCCAACCGTCCGTTGCTGGATCAGAT
CCGGTTGGGTAAAGGAAAAGTTATGGGTATTATCCGGTCTAGAAAGTCATAA

Upstream 100 bases:

>100_bases
CCGTGAAACCGGCGCCAAAGGCAGAGGCTACCGTGAAACCGGAAGCCACACCGAAAGCTACAAGTGAAGAAAAAACAAAT
CTAAATTAAAATCTAATAGT

Downstream 100 bases:

>100_bases
AACAAGAGCAACATGGAAAATAATTTATACGTATCGCCCTCGCCCCACATCCATGGAGGCGACAGCATAAGCAAAAATAT
GTATGGTGTACTGATTGCCC

Product: electron transport complex protein RnfC

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLRTFRIGGIHPPENKLSAGKKITALATPKQVIIPLSQHIGAPAQAVVKKGDLVKVGTLLAKAGGFVSANIHSSVSGKVN
KIDNALDASGYKRPAIYIDVEGDEWEETIDRSETLVKECTLSSKEIVDKIAASGIVGLGGATFPTQVKLMPPPGNKAEIV
IINAVECEPYLTSDHSLMLEKGEQILVGVTILMKAVNVNKAVIGIENNKPDAIAHLQKLAVNYKGIEIMPLKVQYPQGGE
KQLIDAVIRRQVKSGALPISAGAVVQNVGTAYAVYEAVQKNKPLFERVVTITGKAVANPSNFLVRMGTPISCLIEAAGGL
PENTGKIIGGGPMMGKALISAEVPVCKGSSGVLLLTTEESVRKPIRDCIRCAKCVGVCPMGLNPTLLMNATEFQNWELAE
KNYITDCIECGSCSYTCPANRPLLDQIRLGKGKVMGIIRSRKS

Sequences:

>Translated_443_residues
MLRTFRIGGIHPPENKLSAGKKITALATPKQVIIPLSQHIGAPAQAVVKKGDLVKVGTLLAKAGGFVSANIHSSVSGKVN
KIDNALDASGYKRPAIYIDVEGDEWEETIDRSETLVKECTLSSKEIVDKIAASGIVGLGGATFPTQVKLMPPPGNKAEIV
IINAVECEPYLTSDHSLMLEKGEQILVGVTILMKAVNVNKAVIGIENNKPDAIAHLQKLAVNYKGIEIMPLKVQYPQGGE
KQLIDAVIRRQVKSGALPISAGAVVQNVGTAYAVYEAVQKNKPLFERVVTITGKAVANPSNFLVRMGTPISCLIEAAGGL
PENTGKIIGGGPMMGKALISAEVPVCKGSSGVLLLTTEESVRKPIRDCIRCAKCVGVCPMGLNPTLLMNATEFQNWELAE
KNYITDCIECGSCSYTCPANRPLLDQIRLGKGKVMGIIRSRKS
>Mature_443_residues
MLRTFRIGGIHPPENKLSAGKKITALATPKQVIIPLSQHIGAPAQAVVKKGDLVKVGTLLAKAGGFVSANIHSSVSGKVN
KIDNALDASGYKRPAIYIDVEGDEWEETIDRSETLVKECTLSSKEIVDKIAASGIVGLGGATFPTQVKLMPPPGNKAEIV
IINAVECEPYLTSDHSLMLEKGEQILVGVTILMKAVNVNKAVIGIENNKPDAIAHLQKLAVNYKGIEIMPLKVQYPQGGE
KQLIDAVIRRQVKSGALPISAGAVVQNVGTAYAVYEAVQKNKPLFERVVTITGKAVANPSNFLVRMGTPISCLIEAAGGL
PENTGKIIGGGPMMGKALISAEVPVCKGSSGVLLLTTEESVRKPIRDCIRCAKCVGVCPMGLNPTLLMNATEFQNWELAE
KNYITDCIECGSCSYTCPANRPLLDQIRLGKGKVMGIIRSRKS

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=441, Percent_Identity=38.3219954648526, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 47140; Mature: 47140

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRTFRIGGIHPPENKLSAGKKITALATPKQVIIPLSQHIGAPAQAVVKKGDLVKVGTLL
CCEEEECCCCCCCHHHHCCCCEEEEECCCCCEEEEHHHHCCCCHHHHHCCCCEEEHHHHH
AKAGGFVSANIHSSVSGKVNKIDNALDASGYKRPAIYIDVEGDEWEETIDRSETLVKECT
HHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
LSSKEIVDKIAASGIVGLGGATFPTQVKLMPPPGNKAEIVIINAVECEPYLTSDHSLMLE
CCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCEEEEEEEEEECCCEECCCCCEEEC
KGEQILVGVTILMKAVNVNKAVIGIENNKPDAIAHLQKLAVNYKGIEIMPLKVQYPQGGE
CCCEEEEEHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCH
KQLIDAVIRRQVKSGALPISAGAVVQNVGTAYAVYEAVQKNKPLFERVVTITGKAVANPS
HHHHHHHHHHHHHCCCCEEECCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCEECCCC
NFLVRMGTPISCLIEAAGGLPENTGKIIGGGPMMGKALISAEVPVCKGSSGVLLLTTEES
CEEEECCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCEEECCCCEEEEECCHH
VRKPIRDCIRCAKCVGVCPMGLNPTLLMNATEFQNWELAEKNYITDCIECGSCSYTCPAN
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEECCCC
RPLLDQIRLGKGKVMGIIRSRKS
CCHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MLRTFRIGGIHPPENKLSAGKKITALATPKQVIIPLSQHIGAPAQAVVKKGDLVKVGTLL
CCEEEECCCCCCCHHHHCCCCEEEEECCCCCEEEEHHHHCCCCHHHHHCCCCEEEHHHHH
AKAGGFVSANIHSSVSGKVNKIDNALDASGYKRPAIYIDVEGDEWEETIDRSETLVKECT
HHCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHC
LSSKEIVDKIAASGIVGLGGATFPTQVKLMPPPGNKAEIVIINAVECEPYLTSDHSLMLE
CCHHHHHHHHHHCCEEECCCCCCCCEEEEECCCCCCEEEEEEEEEECCCEECCCCCEEEC
KGEQILVGVTILMKAVNVNKAVIGIENNKPDAIAHLQKLAVNYKGIEIMPLKVQYPQGGE
CCCEEEEEHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCH
KQLIDAVIRRQVKSGALPISAGAVVQNVGTAYAVYEAVQKNKPLFERVVTITGKAVANPS
HHHHHHHHHHHHHCCCCEEECCHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCEECCCC
NFLVRMGTPISCLIEAAGGLPENTGKIIGGGPMMGKALISAEVPVCKGSSGVLLLTTEES
CEEEECCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHCCCCCEEECCCCEEEEECCHH
VRKPIRDCIRCAKCVGVCPMGLNPTLLMNATEFQNWELAEKNYITDCIECGSCSYTCPAN
HHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCCEECCCC
RPLLDQIRLGKGKVMGIIRSRKS
CCHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]