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The map label for this gene is tolC [H]

Identifier: 150007002

GI number: 150007002

Start: 385915

End: 387306

Strand: Reverse

Name: tolC [H]

Synonym: BDI_0338

Alternate gene names: 150007002

Gene position: 387306-385915 (Counterclockwise)

Preceding gene: 150007003

Following gene: 150007001

Centisome position: 8.05

GC content: 46.98

Gene sequence:

>1392_bases
ATGAAACGATTATTGATGAGTAGAAAAATGATGTTGGTCACATTTATGGTATTACCTTTTCTGGCGGCGAGACCGGTTCT
GGCTCAAGACACCCTAAAACTGGATCTTGGGAAAGCGCTAGAGATCGCCCTTAGCGAGAACCCGACCGTAAAAGTTGCCG
ATAAAGAAATACAGAAGAAAAAGTACGCACGTAAAGGCTCGTACGCTTCTCTATTTCCGCAGATCAGTTTTGCGGGGGAC
TACAACCGTACCTTGAAGAAACAGGTGATGTATATGGATTTCGACATGGGTGACATGGGAGGCGGTAGCTTGCCCGAAGG
CACGGACATGTCGAGTATGGACGAGGGTTTCGAGGTAGGACGTAGCAACAACTGGAGCTTGGGCTTCAACGCTTCCATGC
CACTGGTGAACGCCTCCTTATGGAAAAGCTTGAGTATCTCGGCCTTAGACGTGGAATTGGCGGTGGAGCAAGCTCGCTCT
TCTAAAATAGCGATGGTGAACCAAGTGAAAAAGAGCTTTTACGCCGTCCTTCTGGCAAGCGACTCTTACCGGGTATTCAA
GCAAAGTTACGACAATGCCATGGAGAATTATCTGGATATCAAACGGAAGTATGAACAGGGAACCGTAGCTGAATACGACT
TGATACGTGCCGATGTGACGGTAAAGAATACAGAGCCTAATCTGTTGCAGGCAGAGAACTCCCTTACCTTGGCTAAATGG
CAGTTAAAAGCGTTATTGGGCATGGATCTGGATTTAAATATCGATTGCGACGGACAACTGACGGATTTCAAGTCCAGCCT
CTTCGCCGACTATCTATCCACGGATACAACCTTATCCGAGAATACGGACTTGAAACAATTAGACCTTCAGGCGGAACAGC
TGAAAAAGACGCTGGTCATGCAGAAATTCGATTATCTGCCGACCTTGTCGCTGACCGGTCTATATCAATGGAGCGCCATG
AACAATGACTTCAAGTTCAAGAATTACAGATGGAACCCCTATTCGATGATCGGTATCACGTTATCGGTTCCTATCTTCTC
CGGAGGTAGTAAATTTTATAAGGTAAAGCAGACGAAAGTCTCCATCGACCAATTAAACCTGCAACGGGACGATACCAAGC
GCAATTTGCAGTTGGCCGTTAAACAGTACATAGATAATATGAATACCTGTATCAAGAAGTTCGACGCCGCCCAAAAGGGA
GTCGAGCAAGCGGAACGGGGATATATGATCTCGCAAAAACGGTACGACACCGGAGCCGGTACATGGCTGGAGATGAACGA
TGCCGAACTTGCCTTGACGCAGGCCCGGTTGAACTTCAACCAATCTATCTACGACTATATGGTAGCGAAGGCGGATCTGG
AGAAAGTATTGGGTAACGAACAGATGAATTGA

Upstream 100 bases:

>100_bases
CGAGAAGGGAAGAGCGATCCTAGACCGATTCGATCTCAAACAATCCTATGATTTATAGCGAACACTTAATGAGTTTAATC
ACAGACAAAATCTATCCTAA

Downstream 100 bases:

>100_bases
AAATTAAAAATTGAAAGTTTAATAGATAATAATTAGGAACAATTATATGAAGACAAGTCAAATGCTGAAAGTAATACCGA
TAGCTACATTGGCCCTGCTG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKRLLMSRKMMLVTFMVLPFLAARPVLAQDTLKLDLGKALEIALSENPTVKVADKEIQKKKYARKGSYASLFPQISFAGD
YNRTLKKQVMYMDFDMGDMGGGSLPEGTDMSSMDEGFEVGRSNNWSLGFNASMPLVNASLWKSLSISALDVELAVEQARS
SKIAMVNQVKKSFYAVLLASDSYRVFKQSYDNAMENYLDIKRKYEQGTVAEYDLIRADVTVKNTEPNLLQAENSLTLAKW
QLKALLGMDLDLNIDCDGQLTDFKSSLFADYLSTDTTLSENTDLKQLDLQAEQLKKTLVMQKFDYLPTLSLTGLYQWSAM
NNDFKFKNYRWNPYSMIGITLSVPIFSGGSKFYKVKQTKVSIDQLNLQRDDTKRNLQLAVKQYIDNMNTCIKKFDAAQKG
VEQAERGYMISQKRYDTGAGTWLEMNDAELALTQARLNFNQSIYDYMVAKADLEKVLGNEQMN

Sequences:

>Translated_463_residues
MKRLLMSRKMMLVTFMVLPFLAARPVLAQDTLKLDLGKALEIALSENPTVKVADKEIQKKKYARKGSYASLFPQISFAGD
YNRTLKKQVMYMDFDMGDMGGGSLPEGTDMSSMDEGFEVGRSNNWSLGFNASMPLVNASLWKSLSISALDVELAVEQARS
SKIAMVNQVKKSFYAVLLASDSYRVFKQSYDNAMENYLDIKRKYEQGTVAEYDLIRADVTVKNTEPNLLQAENSLTLAKW
QLKALLGMDLDLNIDCDGQLTDFKSSLFADYLSTDTTLSENTDLKQLDLQAEQLKKTLVMQKFDYLPTLSLTGLYQWSAM
NNDFKFKNYRWNPYSMIGITLSVPIFSGGSKFYKVKQTKVSIDQLNLQRDDTKRNLQLAVKQYIDNMNTCIKKFDAAQKG
VEQAERGYMISQKRYDTGAGTWLEMNDAELALTQARLNFNQSIYDYMVAKADLEKVLGNEQMN
>Mature_463_residues
MKRLLMSRKMMLVTFMVLPFLAARPVLAQDTLKLDLGKALEIALSENPTVKVADKEIQKKKYARKGSYASLFPQISFAGD
YNRTLKKQVMYMDFDMGDMGGGSLPEGTDMSSMDEGFEVGRSNNWSLGFNASMPLVNASLWKSLSISALDVELAVEQARS
SKIAMVNQVKKSFYAVLLASDSYRVFKQSYDNAMENYLDIKRKYEQGTVAEYDLIRADVTVKNTEPNLLQAENSLTLAKW
QLKALLGMDLDLNIDCDGQLTDFKSSLFADYLSTDTTLSENTDLKQLDLQAEQLKKTLVMQKFDYLPTLSLTGLYQWSAM
NNDFKFKNYRWNPYSMIGITLSVPIFSGGSKFYKVKQTKVSIDQLNLQRDDTKRNLQLAVKQYIDNMNTCIKKFDAAQKG
VEQAERGYMISQKRYDTGAGTWLEMNDAELALTQARLNFNQSIYDYMVAKADLEKVLGNEQMN

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52525; Mature: 52525

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLMSRKMMLVTFMVLPFLAARPVLAQDTLKLDLGKALEIALSENPTVKVADKEIQKK
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHCHHEEECCCEEEEEECCCCEEEECHHHHHHH
KYARKGSYASLFPQISFAGDYNRTLKKQVMYMDFDMGDMGGGSLPEGTDMSSMDEGFEVG
HHHHCCCCHHHCCCEEECCCHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCC
RSNNWSLGFNASMPLVNASLWKSLSISALDVELAVEQARSSKIAMVNQVKKSFYAVLLAS
CCCCCEEECCCCCCCEEHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEC
DSYRVFKQSYDNAMENYLDIKRKYEQGTVAEYDLIRADVTVKNTEPNLLQAENSLTLAKW
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCEEECCCCEEEHHH
QLKALLGMDLDLNIDCDGQLTDFKSSLFADYLSTDTTLSENTDLKQLDLQAEQLKKTLVM
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHH
QKFDYLPTLSLTGLYQWSAMNNDFKFKNYRWNPYSMIGITLSVPIFSGGSKFYKVKQTKV
HHHCCCCCEEECCEEEEECCCCCCEECCCCCCCEEEEEEEEEEEEECCCCCEEEEEEEEE
SIDQLNLQRDDTKRNLQLAVKQYIDNMNTCIKKFDAAQKGVEQAERGYMISQKRYDTGAG
EHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
TWLEMNDAELALTQARLNFNQSIYDYMVAKADLEKVLGNEQMN
CEEEECCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRLLMSRKMMLVTFMVLPFLAARPVLAQDTLKLDLGKALEIALSENPTVKVADKEIQKK
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHCHHEEECCCEEEEEECCCCEEEECHHHHHHH
KYARKGSYASLFPQISFAGDYNRTLKKQVMYMDFDMGDMGGGSLPEGTDMSSMDEGFEVG
HHHHCCCCHHHCCCEEECCCHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHCC
RSNNWSLGFNASMPLVNASLWKSLSISALDVELAVEQARSSKIAMVNQVKKSFYAVLLAS
CCCCCEEECCCCCCCEEHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEC
DSYRVFKQSYDNAMENYLDIKRKYEQGTVAEYDLIRADVTVKNTEPNLLQAENSLTLAKW
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCCEEECCCCEEEHHH
QLKALLGMDLDLNIDCDGQLTDFKSSLFADYLSTDTTLSENTDLKQLDLQAEQLKKTLVM
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHH
QKFDYLPTLSLTGLYQWSAMNNDFKFKNYRWNPYSMIGITLSVPIFSGGSKFYKVKQTKV
HHHCCCCCEEECCEEEEECCCCCCEECCCCCCCEEEEEEEEEEEEECCCCCEEEEEEEEE
SIDQLNLQRDDTKRNLQLAVKQYIDNMNTCIKKFDAAQKGVEQAERGYMISQKRYDTGAG
EHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCC
TWLEMNDAELALTQARLNFNQSIYDYMVAKADLEKVLGNEQMN
CEEEECCCCEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]