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The map label for this gene is def

Identifier: 150006541

GI number: 150006541

Start: 4997505

End: 4998059

Strand: Reverse

Name: def

Synonym: BVU_4061

Alternate gene names: 150006541

Gene position: 4998059-4997505 (Counterclockwise)

Preceding gene: 150006542

Following gene: 150006540

Centisome position: 96.8

GC content: 43.24

Gene sequence:

>555_bases
ATGATATTACCTATTTATGTGTACGGACAACCGGTGTTGCGTAAAGAAGCAGAGGATATTACCCCGGATTACCCTAATTT
GAAAGAGTTGATAGCAAATATGTTTGAAACCATGAATCGTGCAGATGGTGTGGGCTTGGCTGCTCCGCAAATAGGTCTGC
CTATTCGTGTTGTAACTATTGATTTGGATGTGATGTCTGATGACTTGCCCGAATTCAAGGATTTCCGCCGTGCCTATATA
AATCCTCATATTTTAGAAGTGGGTGGAGAGGAAGTCAGCATGGAAGAAGGTTGTTTGAGTTTGCCCGGTATTCATGAAGC
GGTGAAACGTCCGGACCGTATTCATGTGACTTATTTGGATGAGGAACTGAAAGAACATGATGAGTGGGTGGAAGGTTATT
TGGCAAGGGTCATGCAGCATGAATTTGATCATCTGGACGGAAAAATGTTCATTGATCATTTGTCTGCATTACGCAAACAG
ATGATTAAAGGAAAGCTGGGGGCCATGCTGAAGGGCAAGGCGCGTTGCTCCTATAAGGTCAAGACTATAAAATGA

Upstream 100 bases:

>100_bases
AGAACAAAGCCTTGGTTGATGAAATCAGTGCTACGATAATCTTACAGTCTTATTTGGAGTCGAAGAAATATATTTAATAA
ATAAAAACAGATTAAGAAAA

Downstream 100 bases:

>100_bases
TAACTCCGCAATTTTGATCTATGCGCCACCAGTGGCGCATTTGTGGGAAAACCGTATTTTTATAAATACAAAATGATAAA
ACGGATACTCTATACATTAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MILPIYVYGQPVLRKEAEDITPDYPNLKELIANMFETMNRADGVGLAAPQIGLPIRVVTIDLDVMSDDLPEFKDFRRAYI
NPHILEVGGEEVSMEEGCLSLPGIHEAVKRPDRIHVTYLDEELKEHDEWVEGYLARVMQHEFDHLDGKMFIDHLSALRKQ
MIKGKLGAMLKGKARCSYKVKTIK

Sequences:

>Translated_184_residues
MILPIYVYGQPVLRKEAEDITPDYPNLKELIANMFETMNRADGVGLAAPQIGLPIRVVTIDLDVMSDDLPEFKDFRRAYI
NPHILEVGGEEVSMEEGCLSLPGIHEAVKRPDRIHVTYLDEELKEHDEWVEGYLARVMQHEFDHLDGKMFIDHLSALRKQ
MIKGKLGAMLKGKARCSYKVKTIK
>Mature_184_residues
MILPIYVYGQPVLRKEAEDITPDYPNLKELIANMFETMNRADGVGLAAPQIGLPIRVVTIDLDVMSDDLPEFKDFRRAYI
NPHILEVGGEEVSMEEGCLSLPGIHEAVKRPDRIHVTYLDEELKEHDEWVEGYLARVMQHEFDHLDGKMFIDHLSALRKQ
MIKGKLGAMLKGKARCSYKVKTIK

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=160, Percent_Identity=30.625, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=37.8048780487805, Blast_Score=108, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=31.0126582278481, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=29.746835443038, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_BACV8 (A6L7J9)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001301285.1
- ProteinModelPortal:   A6L7J9
- SMR:   A6L7J9
- STRING:   A6L7J9
- GeneID:   5305020
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_4061
- NMPDR:   fig|435590.6.peg.3775
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   MILPINI
- ProtClustDB:   PRK00150
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21020; Mature: 21020

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPIYVYGQPVLRKEAEDITPDYPNLKELIANMFETMNRADGVGLAAPQIGLPIRVVTI
CEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
DLDVMSDDLPEFKDFRRAYINPHILEVGGEEVSMEEGCLSLPGIHEAVKRPDRIHVTYLD
EEEHHCCCCHHHHHHHHHHCCCEEEECCCCCCCHHCCCCCCCCHHHHHCCCCEEEEEEHH
EELKEHDEWVEGYLARVMQHEFDHLDGKMFIDHLSALRKQMIKGKLGAMLKGKARCSYKV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
KTIK
EECC
>Mature Secondary Structure
MILPIYVYGQPVLRKEAEDITPDYPNLKELIANMFETMNRADGVGLAAPQIGLPIRVVTI
CEEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEE
DLDVMSDDLPEFKDFRRAYINPHILEVGGEEVSMEEGCLSLPGIHEAVKRPDRIHVTYLD
EEEHHCCCCHHHHHHHHHHCCCEEEECCCCCCCHHCCCCCCCCHHHHHCCCCEEEEEEHH
EELKEHDEWVEGYLARVMQHEFDHLDGKMFIDHLSALRKQMIKGKLGAMLKGKARCSYKV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
KTIK
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA