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The map label for this gene is thrS

Identifier: 150006539

GI number: 150006539

Start: 4993402

End: 4995342

Strand: Reverse

Name: thrS

Synonym: BVU_4059

Alternate gene names: 150006539

Gene position: 4995342-4993402 (Counterclockwise)

Preceding gene: 150006540

Following gene: 150006538

Centisome position: 96.75

GC content: 45.34

Gene sequence:

>1941_bases
ATGATAAAGATAACATTCCCCGACGGCTCCGTTCGCGAATATAACGAAGGAGTTACTGGTCTGCAGATTGCAGAAAGTAT
CAGTTCACGGCTGGCACAAGATGTTTTAGCTTGTGGCGTGAACGGTGAAACGATTGATTTATCTCGCCCTATCAATGAAG
ATGCAAACTTCGTTCTTTATAAATGGGAAGACGAACAGGGCAAACATGCTTTCTGGCATACTAGTGCCCACTTGCTGGCC
GAAGCATTGCAAGAACTTTATCCGGGCATTCAGTTTGGTATCGGTCCGGCCATTGAAAACGGTTTTTACTATGATGTAGA
CCCGGGTGAAGCAATCATTAAGGAAGCAGATCTACCCGCTATCGAGGCCAAGATGGCCGAGTTGGCTGCAAAGAAAGAGA
TATTGGTGCGTCAGAGCATCGCTAAAGAGGATGCGTTGAAGAAATTCGGTGAAAGAGGTGAGACTTATAAATGTGAATTG
ATTTCCGAACTGGAGGATGGACACATCACCACTTATACACAAGGTGCGTTTACCGACCTTTGCCGTGGTCCTCACTTGAC
CTCTACCGCTCCTATCAAAGCTATCAAGCTGCTTACTGTAGCCGGTGCTTATTGGAGAGGACAGGAAGACCGCAAGCAGA
TGACACGTATTTATGGTATAACTTTCCCGAAGAAGAAAATGCTGGACGAATATCTGGTAATGTTGGAAGAAGCCAAGAAA
CGCGACCATCGTAAGATAGGTAAGGAAATGGATTTGTTCATGTTTACCGATATGGTGGGCAAGGGACTTCCCATGTGGTT
GCCGAAAGGTACTGCCTTGCGTATCCGTTTGCAGGATTTCTTGCGCCGTATCCAGGCCCGTTATGATTATCAGGAAGTAA
TGTGTCCGCCTATTGGCAACAAGAACCTGTATATCACTTCCGGACACTACGCCAAGTACGGTAAGGACTCGTTCCAGCCT
ATTCATACACCCGAAGAAGGGGAAGAATATTTCCTGAAACCGATGAACTGTCCTCATCACTGTATGATTTATAAGAATTC
TCCACGTTCTTATAAGGACCTGCCGTTGCGTATCGCGGAATTCGGAACCGTATGCCGCTACGAACAAAGTGGTGAATTGC
ACGGACTGACCCGTGTGCGCAGTTTTACACAAGATGATGCTCACTTGTTCTGTCGTCCCGACCAAGTGAAGGAAGAATTC
CTTCGCGTGATGGATATCATCAACATTGTGTTCAAGTCTATGAATTTTGAGAATGTGGAAGCTCAGATTTCTCTTCGTGA
CAAGGTGAACCGCGAAAAGTACATCGGTAGTGATGAGAATTGGGAAAAAGCCGAACAGGCCATTATTGAAGCTTGCGAAG
AAAAAGGCCTGACTGCGAAAGTGGAATATGGTGAGGCTGCATTCTATGGTCCTAAATTGGACTTTATGGTGAAAGACGCT
ATCGGTCGTCGCTGGCAATTGGGAACAATTCAGGTAGACTACAACTTGCCCGAACGCTTCCAGTTGGAATATATGGGCTC
AGATAACCAAAAGCATCGTCCGGTAATGATTCACCGTGCGCCTTTCGGATCAATGGAACGTTTTGTTGCCGTATTGATTG
AGCATACTGCCGGTAAATTCCCGCTGTGGCTGACTCCTGACCAGGTAGCTATTCTGCCTATTTCTGAGAAATTCAATGAG
TATGCGGAACAAGTGAAAGCGGAACTGAGAAAATTCGATGTTCGTGCTATTGTGGATGACCGTAATGAAAAAATCGGCCG
TAAGATTCGTGACAATGAAATGAAGCGTATTCCTTACATGTTGATTGTAGGCGAGAAAGAAGCTGAAAACGGTGAAGTTG
CAGTCCGCAAGCAGGGCGAAGGCGACAAAGGAACCATGAAAATAGAAGAATTCGGAAAAAATCTTGCCGAAGAAGTTTCT
AATATGATAAATAAATGGTAA

Upstream 100 bases:

>100_bases
TTACGGAACGAAAAGAATAACATTTTTTTGCTAAAAAGAGGATAAGTCAAAAAGATTTCGTTATTTTTGCATCCTCAAGA
AAGAATATAAATAGAAGATT

Downstream 100 bases:

>100_bases
CTTTGCAGCCGTTTGCAAAAGAGCGCATTGTTAAACCTTTATGTATTCTTTTGCGTTAAGATAAAGAAATACTAATTAAA
ATAAGAAGAAACAAACAGGA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MIKITFPDGSVREYNEGVTGLQIAESISSRLAQDVLACGVNGETIDLSRPINEDANFVLYKWEDEQGKHAFWHTSAHLLA
EALQELYPGIQFGIGPAIENGFYYDVDPGEAIIKEADLPAIEAKMAELAAKKEILVRQSIAKEDALKKFGERGETYKCEL
ISELEDGHITTYTQGAFTDLCRGPHLTSTAPIKAIKLLTVAGAYWRGQEDRKQMTRIYGITFPKKKMLDEYLVMLEEAKK
RDHRKIGKEMDLFMFTDMVGKGLPMWLPKGTALRIRLQDFLRRIQARYDYQEVMCPPIGNKNLYITSGHYAKYGKDSFQP
IHTPEEGEEYFLKPMNCPHHCMIYKNSPRSYKDLPLRIAEFGTVCRYEQSGELHGLTRVRSFTQDDAHLFCRPDQVKEEF
LRVMDIINIVFKSMNFENVEAQISLRDKVNREKYIGSDENWEKAEQAIIEACEEKGLTAKVEYGEAAFYGPKLDFMVKDA
IGRRWQLGTIQVDYNLPERFQLEYMGSDNQKHRPVMIHRAPFGSMERFVAVLIEHTAGKFPLWLTPDQVAILPISEKFNE
YAEQVKAELRKFDVRAIVDDRNEKIGRKIRDNEMKRIPYMLIVGEKEAENGEVAVRKQGEGDKGTMKIEEFGKNLAEEVS
NMINKW

Sequences:

>Translated_646_residues
MIKITFPDGSVREYNEGVTGLQIAESISSRLAQDVLACGVNGETIDLSRPINEDANFVLYKWEDEQGKHAFWHTSAHLLA
EALQELYPGIQFGIGPAIENGFYYDVDPGEAIIKEADLPAIEAKMAELAAKKEILVRQSIAKEDALKKFGERGETYKCEL
ISELEDGHITTYTQGAFTDLCRGPHLTSTAPIKAIKLLTVAGAYWRGQEDRKQMTRIYGITFPKKKMLDEYLVMLEEAKK
RDHRKIGKEMDLFMFTDMVGKGLPMWLPKGTALRIRLQDFLRRIQARYDYQEVMCPPIGNKNLYITSGHYAKYGKDSFQP
IHTPEEGEEYFLKPMNCPHHCMIYKNSPRSYKDLPLRIAEFGTVCRYEQSGELHGLTRVRSFTQDDAHLFCRPDQVKEEF
LRVMDIINIVFKSMNFENVEAQISLRDKVNREKYIGSDENWEKAEQAIIEACEEKGLTAKVEYGEAAFYGPKLDFMVKDA
IGRRWQLGTIQVDYNLPERFQLEYMGSDNQKHRPVMIHRAPFGSMERFVAVLIEHTAGKFPLWLTPDQVAILPISEKFNE
YAEQVKAELRKFDVRAIVDDRNEKIGRKIRDNEMKRIPYMLIVGEKEAENGEVAVRKQGEGDKGTMKIEEFGKNLAEEVS
NMINKW
>Mature_646_residues
MIKITFPDGSVREYNEGVTGLQIAESISSRLAQDVLACGVNGETIDLSRPINEDANFVLYKWEDEQGKHAFWHTSAHLLA
EALQELYPGIQFGIGPAIENGFYYDVDPGEAIIKEADLPAIEAKMAELAAKKEILVRQSIAKEDALKKFGERGETYKCEL
ISELEDGHITTYTQGAFTDLCRGPHLTSTAPIKAIKLLTVAGAYWRGQEDRKQMTRIYGITFPKKKMLDEYLVMLEEAKK
RDHRKIGKEMDLFMFTDMVGKGLPMWLPKGTALRIRLQDFLRRIQARYDYQEVMCPPIGNKNLYITSGHYAKYGKDSFQP
IHTPEEGEEYFLKPMNCPHHCMIYKNSPRSYKDLPLRIAEFGTVCRYEQSGELHGLTRVRSFTQDDAHLFCRPDQVKEEF
LRVMDIINIVFKSMNFENVEAQISLRDKVNREKYIGSDENWEKAEQAIIEACEEKGLTAKVEYGEAAFYGPKLDFMVKDA
IGRRWQLGTIQVDYNLPERFQLEYMGSDNQKHRPVMIHRAPFGSMERFVAVLIEHTAGKFPLWLTPDQVAILPISEKFNE
YAEQVKAELRKFDVRAIVDDRNEKIGRKIRDNEMKRIPYMLIVGEKEAENGEVAVRKQGEGDKGTMKIEEFGKNLAEEVS
NMINKW

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=633, Percent_Identity=39.8104265402844, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI38570111, Length=633, Percent_Identity=38.5466034755134, Blast_Score=451, Evalue=1e-127,
Organism=Homo sapiens, GI20070344, Length=654, Percent_Identity=35.9327217125382, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI1788013, Length=641, Percent_Identity=41.8096723868955, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71984184, Length=634, Percent_Identity=38.6435331230284, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71984192, Length=634, Percent_Identity=38.6435331230284, Blast_Score=467, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322112, Length=659, Percent_Identity=39.6054628224583, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322654, Length=396, Percent_Identity=38.3838383838384, Blast_Score=286, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24583842, Length=633, Percent_Identity=38.2306477093207, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI28574141, Length=633, Percent_Identity=38.2306477093207, Blast_Score=463, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24583839, Length=633, Percent_Identity=38.2306477093207, Blast_Score=463, Evalue=1e-130,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_BACV8 (A6L7J7)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001301283.1
- ProteinModelPortal:   A6L7J7
- SMR:   A6L7J7
- STRING:   A6L7J7
- GeneID:   5305018
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_4059
- NMPDR:   fig|435590.6.peg.3773
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   GRKWQLG
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 74214; Mature: 74214

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKITFPDGSVREYNEGVTGLQIAESISSRLAQDVLACGVNGETIDLSRPINEDANFVLY
CEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEE
KWEDEQGKHAFWHTSAHLLAEALQELYPGIQFGIGPAIENGFYYDVDPGEAIIKEADLPA
EEECCCCCEEEEHHHHHHHHHHHHHHCCCCEECCCCHHCCCEEEECCCCHHHHHHCCCCH
IEAKMAELAAKKEILVRQSIAKEDALKKFGERGETYKCELISELEDGHITTYTQGAFTDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCEEEECCCHHHHH
CRGPHLTSTAPIKAIKLLTVAGAYWRGQEDRKQMTRIYGITFPKKKMLDEYLVMLEEAKK
HCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RDHRKIGKEMDLFMFTDMVGKGLPMWLPKGTALRIRLQDFLRRIQARYDYQEVMCPPIGN
HHHHHHCCHHHHHHHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHCCHHHHCCCCCCC
KNLYITSGHYAKYGKDSFQPIHTPEEGEEYFLKPMNCPHHCMIYKNSPRSYKDLPLRIAE
CEEEEECCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCEEEEEECCCCCCCCCCEEHHH
FGTVCRYEQSGELHGLTRVRSFTQDDAHLFCRPDQVKEEFLRVMDIINIVFKSMNFENVE
HCCCEEECCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCE
AQISLRDKVNREKYIGSDENWEKAEQAIIEACEEKGLTAKVEYGEAAFYGPKLDFMVKDA
EEEEEHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCHHHHHHH
IGRRWQLGTIQVDYNLPERFQLEYMGSDNQKHRPVMIHRAPFGSMERFVAVLIEHTAGKF
CCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
PLWLTPDQVAILPISEKFNEYAEQVKAELRKFDVRAIVDDRNEKIGRKIRDNEMKRIPYM
CEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCHHHCCHHHHCCEE
LIVGEKEAENGEVAVRKQGEGDKGTMKIEEFGKNLAEEVSNMINKW
EEEECCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKITFPDGSVREYNEGVTGLQIAESISSRLAQDVLACGVNGETIDLSRPINEDANFVLY
CEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCEEEE
KWEDEQGKHAFWHTSAHLLAEALQELYPGIQFGIGPAIENGFYYDVDPGEAIIKEADLPA
EEECCCCCEEEEHHHHHHHHHHHHHHCCCCEECCCCHHCCCEEEECCCCHHHHHHCCCCH
IEAKMAELAAKKEILVRQSIAKEDALKKFGERGETYKCELISELEDGHITTYTQGAFTDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCCCEEEECCCHHHHH
CRGPHLTSTAPIKAIKLLTVAGAYWRGQEDRKQMTRIYGITFPKKKMLDEYLVMLEEAKK
HCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RDHRKIGKEMDLFMFTDMVGKGLPMWLPKGTALRIRLQDFLRRIQARYDYQEVMCPPIGN
HHHHHHCCHHHHHHHHHHHHCCCCEECCCCCEEEEEHHHHHHHHHHHCCHHHHCCCCCCC
KNLYITSGHYAKYGKDSFQPIHTPEEGEEYFLKPMNCPHHCMIYKNSPRSYKDLPLRIAE
CEEEEECCCCCCCCCCCCCCCCCCCCCCHHEECCCCCCCEEEEEECCCCCCCCCCEEHHH
FGTVCRYEQSGELHGLTRVRSFTQDDAHLFCRPDQVKEEFLRVMDIINIVFKSMNFENVE
HCCCEEECCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCE
AQISLRDKVNREKYIGSDENWEKAEQAIIEACEEKGLTAKVEYGEAAFYGPKLDFMVKDA
EEEEEHHHCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCHHHHHHH
IGRRWQLGTIQVDYNLPERFQLEYMGSDNQKHRPVMIHRAPFGSMERFVAVLIEHTAGKF
CCCEEEEEEEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCC
PLWLTPDQVAILPISEKFNEYAEQVKAELRKFDVRAIVDDRNEKIGRKIRDNEMKRIPYM
CEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCHHHCCHHHHCCEE
LIVGEKEAENGEVAVRKQGEGDKGTMKIEEFGKNLAEEVSNMINKW
EEEECCCCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA