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The map label for this gene is mhpE [C]

Identifier: 150006516

GI number: 150006516

Start: 4969031

End: 4970608

Strand: Reverse

Name: mhpE [C]

Synonym: BVU_4036

Alternate gene names: 150006516

Gene position: 4970608-4969031 (Counterclockwise)

Preceding gene: 150006517

Following gene: 150006515

Centisome position: 96.27

GC content: 38.91

Gene sequence:

>1578_bases
ATGAGCAAAATCCAACTATTAGATTGTACTTTGCGTGATGGAGGGTACATCAATAATTTCCGATTCGGTAAATATGCCAT
ACGGAAAATTCTTCAACAACTGACCCATGCAGGTTTGGATATAGTGGAATGTGGATTTTTGGAGGATGGTGAATACGACG
AGGATACCTCCATTTTTAATGCCGCAGAGCAGATAGCCTCCCTGTTACCTAATGAACATGGACGTACCATGTTTGTAGCG
ATGGTCTGTTATGGTGAATACGATCTGAGTCAGCTGAGTCCTTGTACTAGAAATTCCATAGATGGAATACGGGTCACTTT
TCATTATGATGAAGTGGAGGGTGCTTTGGAATACTGTCGGGAAATAAAAGAAAAAGGCTATAAGGTTTTTGTGCAACCGG
TAGGCACGTCCAGTTATACTGATATCCAGCTTCTCCATCTGATAGAGAAGGTGAATGAGTTGCAACCCTATTCCTTTTAT
CTGGTAGACACTTTGGGACTGATGCACAAGAATGATATAGCCAGATTTTTTTATCTGATCAATACTAATCTGGACAAGTC
AATAAATATGGGTTTTCATTCCCACAATAACTTACAGCTTTCCTTTTCCAACTCTCAGGAGTTTTTTGAATATGTAGGCG
ACCGTGTCATTTCGTTGGATGCTTCTATCTATGGAATGGGACGAGGGGCCGGAAACCTGAATACGGAACTGATTGCCAAT
TATGTTAATGACCGTGAAGGGCATATGTATGCTATAGAGCCATTGTTGGAGGTTGTTGATGAATTCATCATTCATATTAG
GCAGAAACATGAGTGGGGGTATACTGTTCCTTATTATTTGGCGGCTATTAATGGTTGTCATCCTAATTATGCCTCTTATC
TGGCTCAGAAACAGACGCTTGCTGTGAAGGAAATTTCTGCCATATTGCATATGATTCCTGTCGAAAAGCGTTCTTTATAC
AATAAGGAATTGGTAAATAGGATTTATTTGGAGTATCAATCTCATGAGGTGGATGATACTTATGTCTTAAAGAACTTGAA
AGAAAAAATAGGTGGAAGAAATATTATACTGATGGCTCCAGGAGGTTCAATTACAGACTGTTATGACGAAATTGTCCAGA
AACAGAATTCGGAGAACTGTCTGCTTATTTCTGTGACCTTTGTTCCTGATTTTATGGATTGTGACTTTACTTTCTTGGCT
AATGCCAAGCGGTATGCTACGACTTTTAATCCGATGAAAAAGGAGGTGAGTTTGATTTATACGTCCAATATTCGTGCAAA
TCAAGAACAATCTGGCTATTGTGTCAATTATGTTTCTTTGCTGAACGAAGTGGACATTATTCGTGATAATTCGGCTCTGA
TGGCTTTGAACCTTTTACAGAAACTGTCTCCTCGAAAAGTTTATCTGGCAGGTATGGACGGTTACTCTATGAAAAAGGGA
AACTACTATCAGGAGCGTTTGAATCTGATTCAGGATAGGGATTATGTCTTGGAAATGAATAAAGCGATAAAGCATCGCAT
CAGTGAAATAGGAAAGGTTATGGATATTGAATTTTTAACTCCGTCTCTATATGAATGA

Upstream 100 bases:

>100_bases
TAGAGATTCCAGCTGAAACTTTATCTGTAGATACGCAGGCTGATGTGAAAATTGTGACAGAAGTCTTAGAGCGCAGGGTT
AAAAATAAGGAGATCGTATT

Downstream 100 bases:

>100_bases
ACCGGATATTTGTAGAAGCATACATGAACTGATTATGCTTCATTTGCCTGAAATTCTCTCCTTACGGAATACTTCAGAGT
TGAAATCCGATCATACCTTT

Product: putative 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MSKIQLLDCTLRDGGYINNFRFGKYAIRKILQQLTHAGLDIVECGFLEDGEYDEDTSIFNAAEQIASLLPNEHGRTMFVA
MVCYGEYDLSQLSPCTRNSIDGIRVTFHYDEVEGALEYCREIKEKGYKVFVQPVGTSSYTDIQLLHLIEKVNELQPYSFY
LVDTLGLMHKNDIARFFYLINTNLDKSINMGFHSHNNLQLSFSNSQEFFEYVGDRVISLDASIYGMGRGAGNLNTELIAN
YVNDREGHMYAIEPLLEVVDEFIIHIRQKHEWGYTVPYYLAAINGCHPNYASYLAQKQTLAVKEISAILHMIPVEKRSLY
NKELVNRIYLEYQSHEVDDTYVLKNLKEKIGGRNIILMAPGGSITDCYDEIVQKQNSENCLLISVTFVPDFMDCDFTFLA
NAKRYATTFNPMKKEVSLIYTSNIRANQEQSGYCVNYVSLLNEVDIIRDNSALMALNLLQKLSPRKVYLAGMDGYSMKKG
NYYQERLNLIQDRDYVLEMNKAIKHRISEIGKVMDIEFLTPSLYE

Sequences:

>Translated_525_residues
MSKIQLLDCTLRDGGYINNFRFGKYAIRKILQQLTHAGLDIVECGFLEDGEYDEDTSIFNAAEQIASLLPNEHGRTMFVA
MVCYGEYDLSQLSPCTRNSIDGIRVTFHYDEVEGALEYCREIKEKGYKVFVQPVGTSSYTDIQLLHLIEKVNELQPYSFY
LVDTLGLMHKNDIARFFYLINTNLDKSINMGFHSHNNLQLSFSNSQEFFEYVGDRVISLDASIYGMGRGAGNLNTELIAN
YVNDREGHMYAIEPLLEVVDEFIIHIRQKHEWGYTVPYYLAAINGCHPNYASYLAQKQTLAVKEISAILHMIPVEKRSLY
NKELVNRIYLEYQSHEVDDTYVLKNLKEKIGGRNIILMAPGGSITDCYDEIVQKQNSENCLLISVTFVPDFMDCDFTFLA
NAKRYATTFNPMKKEVSLIYTSNIRANQEQSGYCVNYVSLLNEVDIIRDNSALMALNLLQKLSPRKVYLAGMDGYSMKKG
NYYQERLNLIQDRDYVLEMNKAIKHRISEIGKVMDIEFLTPSLYE
>Mature_524_residues
SKIQLLDCTLRDGGYINNFRFGKYAIRKILQQLTHAGLDIVECGFLEDGEYDEDTSIFNAAEQIASLLPNEHGRTMFVAM
VCYGEYDLSQLSPCTRNSIDGIRVTFHYDEVEGALEYCREIKEKGYKVFVQPVGTSSYTDIQLLHLIEKVNELQPYSFYL
VDTLGLMHKNDIARFFYLINTNLDKSINMGFHSHNNLQLSFSNSQEFFEYVGDRVISLDASIYGMGRGAGNLNTELIANY
VNDREGHMYAIEPLLEVVDEFIIHIRQKHEWGYTVPYYLAAINGCHPNYASYLAQKQTLAVKEISAILHMIPVEKRSLYN
KELVNRIYLEYQSHEVDDTYVLKNLKEKIGGRNIILMAPGGSITDCYDEIVQKQNSENCLLISVTFVPDFMDCDFTFLAN
AKRYATTFNPMKKEVSLIYTSNIRANQEQSGYCVNYVSLLNEVDIIRDNSALMALNLLQKLSPRKVYLAGMDGYSMKKGN
YYQERLNLIQDRDYVLEMNKAIKHRISEIGKVMDIEFLTPSLYE

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 60313; Mature: 60181

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIQLLDCTLRDGGYINNFRFGKYAIRKILQQLTHAGLDIVECGFLEDGEYDEDTSIFN
CCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
AAEQIASLLPNEHGRTMFVAMVCYGEYDLSQLSPCTRNSIDGIRVTFHYDEVEGALEYCR
HHHHHHHHCCCCCCCEEEEEEEEECCCCHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHH
EIKEKGYKVFVQPVGTSSYTDIQLLHLIEKVNELQPYSFYLVDTLGLMHKNDIARFFYLI
HHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEEEEEEE
NTNLDKSINMGFHSHNNLQLSFSNSQEFFEYVGDRVISLDASIYGMGRGAGNLNTELIAN
ECCCCCCCCCCEECCCCEEEEECCHHHHHHHHCCEEEEECCEEEECCCCCCCCCHHHHHH
YVNDREGHMYAIEPLLEVVDEFIIHIRQKHEWGYTVPYYLAAINGCHPNYASYLAQKQTL
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
AVKEISAILHMIPVEKRSLYNKELVNRIYLEYQSHEVDDTYVLKNLKEKIGGRNIILMAP
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECC
GGSITDCYDEIVQKQNSENCLLISVTFVPDFMDCDFTFLANAKRYATTFNPMKKEVSLIY
CCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEECCCHHHHHCCCHHHHCEEEEE
TSNIRANQEQSGYCVNYVSLLNEVDIIRDNSALMALNLLQKLSPRKVYLAGMDGYSMKKG
ECCCCCCCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCC
NYYQERLNLIQDRDYVLEMNKAIKHRISEIGKVMDIEFLTPSLYE
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC
>Mature Secondary Structure 
SKIQLLDCTLRDGGYINNFRFGKYAIRKILQQLTHAGLDIVECGFLEDGEYDEDTSIFN
CCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHH
AAEQIASLLPNEHGRTMFVAMVCYGEYDLSQLSPCTRNSIDGIRVTFHYDEVEGALEYCR
HHHHHHHHCCCCCCCEEEEEEEEECCCCHHHCCCCCCCCCCCEEEEEEEHHHHHHHHHHH
EIKEKGYKVFVQPVGTSSYTDIQLLHLIEKVNELQPYSFYLVDTLGLMHKNDIARFFYLI
HHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCEEEEEEE
NTNLDKSINMGFHSHNNLQLSFSNSQEFFEYVGDRVISLDASIYGMGRGAGNLNTELIAN
ECCCCCCCCCCEECCCCEEEEECCHHHHHHHHCCEEEEECCEEEECCCCCCCCCHHHHHH
YVNDREGHMYAIEPLLEVVDEFIIHIRQKHEWGYTVPYYLAAINGCHPNYASYLAQKQTL
HHCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHH
AVKEISAILHMIPVEKRSLYNKELVNRIYLEYQSHEVDDTYVLKNLKEKIGGRNIILMAP
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEECC
GGSITDCYDEIVQKQNSENCLLISVTFVPDFMDCDFTFLANAKRYATTFNPMKKEVSLIY
CCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCEECCCHHHHHCCCHHHHCEEEEE
TSNIRANQEQSGYCVNYVSLLNEVDIIRDNSALMALNLLQKLSPRKVYLAGMDGYSMKKG
ECCCCCCCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCCEEEEECCCCCCCCCC
NYYQERLNLIQDRDYVLEMNKAIKHRISEIGKVMDIEFLTPSLYE
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA