Click here to switch to the map view.

The map label for this gene is hpaIIR [H]

Identifier: 150006473

GI number: 150006473

Start: 4912075

End: 4913163

Strand: Reverse

Name: hpaIIR [H]

Synonym: BVU_3992

Alternate gene names: 150006473

Gene position: 4913163-4912075 (Counterclockwise)

Preceding gene: 150006477

Following gene: 150006468

Centisome position: 95.16

GC content: 42.42

Gene sequence:

>1089_bases
ATGGCATTTGAAGCGACAAAGAGAGAGTGGGGCGAGCTTTACGCCTTTTTCCGCTTGTTAGCGGACGGATATGTGTATGC
AGGTACGCCTGATGTGAAAAGGAATGAAGTGCAAAAGCTTCCTGTGGCGATGGTACAGCGTGAGGAACATGACGGGACAC
GCCGTTATATTCTGGAGGATGAGGCGACCGTACGTATTTGTGGGGAAAAGATAGATAAGCAGATACCTCGTGAGGATTTT
GCGGCTGTGGCAGAGTTGGTTTTTGCTGCTGTCAAAGAGAGCCGAGAGAATGATGTGATGTCTCCCGACGGTGTAGAAGA
ATTTTTAGATGAAGTCGCCATTTATGACTTGGAAGCAAAGACGGATGACCGTACTGATTTCTATGTTGCTTTTTACAGTA
TAGAGGCTCCGTTGGTAGGTTTTTGCGTTCGTTCCCGTTTGGGAACTATGTTCCCGTTGTTGGATGGAGGGCGTACCGCC
AATTTGAAGTTTGAGCAGACAGGTGTTAAATTCGCTACTCCTACCGTAAACAAAATTAATGCTTTTGGTGAAGAAGATGA
TGTGGCAGGACGTATGTTGATGATAGAACGCTTGGGAGGAATCTTAAAATATAATGATGTGGCCGATAAGGTATTCCGTA
GTAATCTTTGCATGATAGATCTTCATTTTCCCCGTATGTTGGGCGAGATGTTGCGTGTCATGCATTTGGATGGTATATCC
AAAGTTAGTGACCTGACAGAAGCAATCAAACAAATCAATCCGTTGAAAATAAAGGATGAATTGATCCATAAGCATAGTTA
TTATGAATATAAGATGAAGCAGTTTCTTATGGCTTTGGCTTTAGGAATGCGTCCTGCCAAGATATTTAATGGGATAGATT
CTGCCATATCCGGTTTTCTGTTTGTAAATGGTAATGGAGAGGTACTTTGTTATCAGAAGGCTGACCGTCAGGTATTTGCC
GATTTCCTGTTTGTTAATTCCCGGTTTGAGAAAAGTTCTACTGAAAAAGATAAATACGGATATCTGGAACGTGAGAACGG
AGTCTATTATTTTAAGCTGAACTTGAAAATCGGTCTGTTGAAGAGATAG

Upstream 100 bases:

>100_bases
TTTAATATTTGCGTTTCTCGAAAACCTGTTTTTTAAGACTTCTTTTGCGTATCTTTGCAGCCGTTAAAAAATAGCAGACG
ATAGTAAAAAGAGAAAATAT

Downstream 100 bases:

>100_bases
GACAATGAAGGAATTTGTATTTTCCTAGAGTTAAAATGTTTTTTACCACAGATTACACAGATTGAAATACGATTTATTGC
TTATTTCAATCTGTATCTGT

Product: type II restriction enzyme HpaII

Products: NA

Alternate protein names: R.HpaII; Endonuclease HpaII; Type II restriction enzyme HpaII [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MAFEATKREWGELYAFFRLLADGYVYAGTPDVKRNEVQKLPVAMVQREEHDGTRRYILEDEATVRICGEKIDKQIPREDF
AAVAELVFAAVKESRENDVMSPDGVEEFLDEVAIYDLEAKTDDRTDFYVAFYSIEAPLVGFCVRSRLGTMFPLLDGGRTA
NLKFEQTGVKFATPTVNKINAFGEEDDVAGRMLMIERLGGILKYNDVADKVFRSNLCMIDLHFPRMLGEMLRVMHLDGIS
KVSDLTEAIKQINPLKIKDELIHKHSYYEYKMKQFLMALALGMRPAKIFNGIDSAISGFLFVNGNGEVLCYQKADRQVFA
DFLFVNSRFEKSSTEKDKYGYLERENGVYYFKLNLKIGLLKR

Sequences:

>Translated_362_residues
MAFEATKREWGELYAFFRLLADGYVYAGTPDVKRNEVQKLPVAMVQREEHDGTRRYILEDEATVRICGEKIDKQIPREDF
AAVAELVFAAVKESRENDVMSPDGVEEFLDEVAIYDLEAKTDDRTDFYVAFYSIEAPLVGFCVRSRLGTMFPLLDGGRTA
NLKFEQTGVKFATPTVNKINAFGEEDDVAGRMLMIERLGGILKYNDVADKVFRSNLCMIDLHFPRMLGEMLRVMHLDGIS
KVSDLTEAIKQINPLKIKDELIHKHSYYEYKMKQFLMALALGMRPAKIFNGIDSAISGFLFVNGNGEVLCYQKADRQVFA
DFLFVNSRFEKSSTEKDKYGYLERENGVYYFKLNLKIGLLKR
>Mature_361_residues
AFEATKREWGELYAFFRLLADGYVYAGTPDVKRNEVQKLPVAMVQREEHDGTRRYILEDEATVRICGEKIDKQIPREDFA
AVAELVFAAVKESRENDVMSPDGVEEFLDEVAIYDLEAKTDDRTDFYVAFYSIEAPLVGFCVRSRLGTMFPLLDGGRTAN
LKFEQTGVKFATPTVNKINAFGEEDDVAGRMLMIERLGGILKYNDVADKVFRSNLCMIDLHFPRMLGEMLRVMHLDGISK
VSDLTEAIKQINPLKIKDELIHKHSYYEYKMKQFLMALALGMRPAKIFNGIDSAISGFLFVNGNGEVLCYQKADRQVFAD
FLFVNSRFEKSSTEKDKYGYLERENGVYYFKLNLKIGLLKR

Specific function: Recognizes the double-stranded sequence CCGG and cleaves after C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019062 [H]

Pfam domain/function: PF09561 RE_HpaII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 41443; Mature: 41312

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEATKREWGELYAFFRLLADGYVYAGTPDVKRNEVQKLPVAMVQREEHDGTRRYILED
CCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHCCHHHHHHHCCCCCEEEEECC
EATVRICGEKIDKQIPREDFAAVAELVFAAVKESRENDVMSPDGVEEFLDEVAIYDLEAK
CCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECC
TDDRTDFYVAFYSIEAPLVGFCVRSRLGTMFPLLDGGRTANLKFEQTGVKFATPTVNKIN
CCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECEEECCCCCEEECCCHHHHH
AFGEEDDVAGRMLMIERLGGILKYNDVADKVFRSNLCMIDLHFPRMLGEMLRVMHLDGIS
CCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCHH
KVSDLTEAIKQINPLKIKDELIHKHSYYEYKMKQFLMALALGMRPAKIFNGIDSAISGFL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEE
FVNGNGEVLCYQKADRQVFADFLFVNSRFEKSSTEKDKYGYLERENGVYYFKLNLKIGLL
EECCCCCEEEEECCCHHHHHHHHHHHCHHCCCCCCCHHCCCEEECCCEEEEEEEEEEEEC
KR
CC
>Mature Secondary Structure 
AFEATKREWGELYAFFRLLADGYVYAGTPDVKRNEVQKLPVAMVQREEHDGTRRYILED
CCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHCCHHHHHHHCCCCCEEEEECC
EATVRICGEKIDKQIPREDFAAVAELVFAAVKESRENDVMSPDGVEEFLDEVAIYDLEAK
CCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEEECC
TDDRTDFYVAFYSIEAPLVGFCVRSRLGTMFPLLDGGRTANLKFEQTGVKFATPTVNKIN
CCCCCEEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCEECEEECCCCCEEECCCHHHHH
AFGEEDDVAGRMLMIERLGGILKYNDVADKVFRSNLCMIDLHFPRMLGEMLRVMHLDGIS
CCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCHH
KVSDLTEAIKQINPLKIKDELIHKHSYYEYKMKQFLMALALGMRPAKIFNGIDSAISGFL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEE
FVNGNGEVLCYQKADRQVFADFLFVNSRFEKSSTEKDKYGYLERENGVYYFKLNLKIGLL
EECCCCCEEEEECCCHHHHHHHHHHHCHHCCCCCCCHHCCCEEECCCEEEEEEEEEEEEC
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7514149 [H]