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The map label for this gene is icaA [H]

Identifier: 150006463

GI number: 150006463

Start: 4900647

End: 4901852

Strand: Direct

Name: icaA [H]

Synonym: BVU_3982

Alternate gene names: 150006463

Gene position: 4900647-4901852 (Clockwise)

Preceding gene: 150006462

Following gene: 150006465

Centisome position: 94.92

GC content: 45.27

Gene sequence:

>1206_bases
ATGAACACTCTTGAAATCCTCTTCTTCGTTCTCCTGTTCATTGTATTCTACACCTATGTGGGATACGGAATCCTGTTGTG
GATATTGGTAAAAATAAAACAAAGTTTTATTTCCTTCTACGATACGGACGAACCGGAGCAAGAAACCTGCCGGAACGGCA
ACAACACCGACCTACCCGAAATCACCCTGTTCATCACCGCCTATAATGAGGAACAGGTAGTGGACGGAAAGATGAAAAAC
TGCCAAGAACTGGATTATCCCAAAGAGAAACTGCATATCGTATGGGTAACGGACGGCAGCAATGACCGAACCAATGAAAA
ATTAAAAGCATATCCCGATGTCACCCTATTGTTCGTCCCCGAAAGAAAAGGAAAAACAGCCGCTATGAACCGGGGCATGG
GATTTGTCACCACACCTTTGGTCATATTCACAGACGCCAATACATTTATTAATCCGCAGGCCGTTCGCGAGATAGTAAAA
TGTTTTAACCATCCGCAGGTGGGATGTGTGGCAGGAGAAAAACGGGTGGACATGTACAGTACCGAAGGAGCGGTTTCCGG
AGGAGAAGGATTGTACTGGAAATATGAATCATGGCTGAAAAAAATGGATTATCAGCTCTATTCCGCCATAGGTGCTGCCG
GAGAATTATTCGCCATCCGCACCCCGTTATATGAGGAAATGCCTGAAGATACCCTACTGGATGATTTTATGTTGTCCCTG
CGTATCGCCATGAAGCAGTATACGATAGCATATTGTGATACGGCATATGCCTTGGAAAGCGGATCGGCCGATATGAAAGA
AGAGGAAAAAAGAAAAGTACGTATCGCGGCAGGAGGATTGCAGTCTGTCTACCGTTTGAAAGAACTGCTGAATCCGCTAC
GTTATGGAATCCTCAGTTTTCAATATGTTTCCCATCGCGTGCTGAGATGGTCGGTAACCCCGGTCGCCTTGTTTCTGCTC
TTTCCCTTGAATATCCTGCTGGTTGTCTGTAGTGAAAGCCTCCCGGTTTATTTTCTTTTTCTTCTGCTGCAATCCGCATT
CTACCTCGGGGGAGTTTATGGCAGCCTCTTGTCTGCCAAGTCCGTAAAGAACAAATTCCTTTATATTCCCTACTATTTCC
TGTTCATGAACATCAATGTGATAAAAGGTTTCTTCTATTTGAAGAGACATGCCGGCGGAACATGGGAAAAGTCCCGCAGG
GCATAA

Upstream 100 bases:

>100_bases
TCTATCAGTCACAAACTGCATATGGACTAGAAAGAGTCCGTCGGTTCTCATGGGAGAACACAGCCAGAGAAATATTGAAT
ATATATAAAGAAATACTAGC

Downstream 100 bases:

>100_bases
TATTTACTCACCAAAATACTTTTTTATCATCTCACGCAAAGCGGGAGCCATGATCGGCTTGGCCATATAATCGTCACATC
CGGCTTCAAAAGCCTTGTCA

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNTLEILFFVLLFIVFYTYVGYGILLWILVKIKQSFISFYDTDEPEQETCRNGNNTDLPEITLFITAYNEEQVVDGKMKN
CQELDYPKEKLHIVWVTDGSNDRTNEKLKAYPDVTLLFVPERKGKTAAMNRGMGFVTTPLVIFTDANTFINPQAVREIVK
CFNHPQVGCVAGEKRVDMYSTEGAVSGGEGLYWKYESWLKKMDYQLYSAIGAAGELFAIRTPLYEEMPEDTLLDDFMLSL
RIAMKQYTIAYCDTAYALESGSADMKEEEKRKVRIAAGGLQSVYRLKELLNPLRYGILSFQYVSHRVLRWSVTPVALFLL
FPLNILLVVCSESLPVYFLFLLLQSAFYLGGVYGSLLSAKSVKNKFLYIPYYFLFMNINVIKGFFYLKRHAGGTWEKSRR
A

Sequences:

>Translated_401_residues
MNTLEILFFVLLFIVFYTYVGYGILLWILVKIKQSFISFYDTDEPEQETCRNGNNTDLPEITLFITAYNEEQVVDGKMKN
CQELDYPKEKLHIVWVTDGSNDRTNEKLKAYPDVTLLFVPERKGKTAAMNRGMGFVTTPLVIFTDANTFINPQAVREIVK
CFNHPQVGCVAGEKRVDMYSTEGAVSGGEGLYWKYESWLKKMDYQLYSAIGAAGELFAIRTPLYEEMPEDTLLDDFMLSL
RIAMKQYTIAYCDTAYALESGSADMKEEEKRKVRIAAGGLQSVYRLKELLNPLRYGILSFQYVSHRVLRWSVTPVALFLL
FPLNILLVVCSESLPVYFLFLLLQSAFYLGGVYGSLLSAKSVKNKFLYIPYYFLFMNINVIKGFFYLKRHAGGTWEKSRR
A
>Mature_401_residues
MNTLEILFFVLLFIVFYTYVGYGILLWILVKIKQSFISFYDTDEPEQETCRNGNNTDLPEITLFITAYNEEQVVDGKMKN
CQELDYPKEKLHIVWVTDGSNDRTNEKLKAYPDVTLLFVPERKGKTAAMNRGMGFVTTPLVIFTDANTFINPQAVREIVK
CFNHPQVGCVAGEKRVDMYSTEGAVSGGEGLYWKYESWLKKMDYQLYSAIGAAGELFAIRTPLYEEMPEDTLLDDFMLSL
RIAMKQYTIAYCDTAYALESGSADMKEEEKRKVRIAAGGLQSVYRLKELLNPLRYGILSFQYVSHRVLRWSVTPVALFLL
FPLNILLVVCSESLPVYFLFLLLQSAFYLGGVYGSLLSAKSVKNKFLYIPYYFLFMNINVIKGFFYLKRHAGGTWEKSRR
A

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=339, Percent_Identity=23.598820058997, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 46040; Mature: 46040

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLEILFFVLLFIVFYTYVGYGILLWILVKIKQSFISFYDTDEPEQETCRNGNNTDLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCE
ITLFITAYNEEQVVDGKMKNCQELDYPKEKLHIVWVTDGSNDRTNEKLKAYPDVTLLFVP
EEEEEEECCCCHHCCCHHHCHHHCCCCCHHEEEEEEECCCCCCCCCHHCCCCCEEEEEEE
ERKGKTAAMNRGMGFVTTPLVIFTDANTFINPQAVREIVKCFNHPQVGCVAGEKRVDMYS
CCCCCHHHHHCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEE
TEGAVSGGEGLYWKYESWLKKMDYQLYSAIGAAGELFAIRTPLYEEMPEDTLLDDFMLSL
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCHHHHHHHHHHH
RIAMKQYTIAYCDTAYALESGSADMKEEEKRKVRIAAGGLQSVYRLKELLNPLRYGILSF
HHHHHHHHHEEECHHHHHCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QYVSHRVLRWSVTPVALFLLFPLNILLVVCSESLPVYFLFLLLQSAFYLGGVYGSLLSAK
HHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKNKFLYIPYYFLFMNINVIKGFFYLKRHAGGTWEKSRRA
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC
>Mature Secondary Structure
MNTLEILFFVLLFIVFYTYVGYGILLWILVKIKQSFISFYDTDEPEQETCRNGNNTDLPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCE
ITLFITAYNEEQVVDGKMKNCQELDYPKEKLHIVWVTDGSNDRTNEKLKAYPDVTLLFVP
EEEEEEECCCCHHCCCHHHCHHHCCCCCHHEEEEEEECCCCCCCCCHHCCCCCEEEEEEE
ERKGKTAAMNRGMGFVTTPLVIFTDANTFINPQAVREIVKCFNHPQVGCVAGEKRVDMYS
CCCCCHHHHHCCCCEEECCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCEEEE
TEGAVSGGEGLYWKYESWLKKMDYQLYSAIGAAGELFAIRTPLYEEMPEDTLLDDFMLSL
CCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHCCCHHHHHHHHHHH
RIAMKQYTIAYCDTAYALESGSADMKEEEKRKVRIAAGGLQSVYRLKELLNPLRYGILSF
HHHHHHHHHEEECHHHHHCCCCCCCCHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHH
QYVSHRVLRWSVTPVALFLLFPLNILLVVCSESLPVYFLFLLLQSAFYLGGVYGSLLSAK
HHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SVKNKFLYIPYYFLFMNINVIKGFFYLKRHAGGTWEKSRRA
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]