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The map label for this gene is hisH [H]

Identifier: 150006332

GI number: 150006332

Start: 4752334

End: 4752924

Strand: Direct

Name: hisH [H]

Synonym: BVU_3851

Alternate gene names: 150006332

Gene position: 4752334-4752924 (Clockwise)

Preceding gene: 150006331

Following gene: 150006333

Centisome position: 92.04

GC content: 45.85

Gene sequence:

>591_bases
ATGAAAATTGCTGTCGTAAAATATAATGCAGGAAACATCTACTCCGTAGACTATGCACTGAAGCGCCTTGGAGTAGAAGC
AACCATCACTTCCGATAAGGAAGTATTGATGAGTGCCGACAAAGTTATCTTCCCCGGTGTGGGCGAAGCCGAAACAACCA
TGAGCCATCTCCGGAAAAACCGGTTGGATGAAGTTATTAAAAACTTAAAACAACCTGTACTAGGTATCTGCCTGGGAATG
CAGCTGATGTGCCGGCACTCCGAAGAAGGAAATGCTGACTGCTTGGGCATCTTTGATACTGATGTAAAGTTGTTCAGCCC
CACCCGGCATGAAGACAAAGTACCCCATATGGGATGGAACACCCTGACTCATGTACGCAGCGACCTGTTCAAAGGATTCA
CCAAAGAAGAATTTGTATACTTCGTACATAGCTTCTATGTGCCTCTGAACGAGTTTACGGCAGCACAAACAGATTATATC
CTCCCCTACAGTTCGGCCTTGCACAAAGATAATTTCTATGCGACACAGTTCCATCCCGAAAAGAGCGGAGCCGTGGGCGA
ACGAATCCTGAGGAACTTTTTAGAATTGTGA

Upstream 100 bases:

>100_bases
AAGGTAAAGATTTGGAAAAAATAGTCCTTTCTCGTGCTGTTCAGAAACATATTGAACGAAAAGTATTGGCTTATAAAAAT
AAAACAGTTATCTTTAGCTG

Downstream 100 bases:

>100_bases
ACCATTACATTATATATAACAATGTATAAGAAGAAATAGATATGATAGAGTTAATTCCTGCCATCGACATTATTGATGGC
AAATGCGTCCGTCTTTCTCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKIAVVKYNAGNIYSVDYALKRLGVEATITSDKEVLMSADKVIFPGVGEAETTMSHLRKNRLDEVIKNLKQPVLGICLGM
QLMCRHSEEGNADCLGIFDTDVKLFSPTRHEDKVPHMGWNTLTHVRSDLFKGFTKEEFVYFVHSFYVPLNEFTAAQTDYI
LPYSSALHKDNFYATQFHPEKSGAVGERILRNFLEL

Sequences:

>Translated_196_residues
MKIAVVKYNAGNIYSVDYALKRLGVEATITSDKEVLMSADKVIFPGVGEAETTMSHLRKNRLDEVIKNLKQPVLGICLGM
QLMCRHSEEGNADCLGIFDTDVKLFSPTRHEDKVPHMGWNTLTHVRSDLFKGFTKEEFVYFVHSFYVPLNEFTAAQTDYI
LPYSSALHKDNFYATQFHPEKSGAVGERILRNFLEL
>Mature_196_residues
MKIAVVKYNAGNIYSVDYALKRLGVEATITSDKEVLMSADKVIFPGVGEAETTMSHLRKNRLDEVIKNLKQPVLGICLGM
QLMCRHSEEGNADCLGIFDTDVKLFSPTRHEDKVPHMGWNTLTHVRSDLFKGFTKEEFVYFVHSFYVPLNEFTAAQTDYI
LPYSSALHKDNFYATQFHPEKSGAVGERILRNFLEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=198, Percent_Identity=44.9494949494949, Blast_Score=182, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=31.5789473684211, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 22270; Mature: 22270

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVVKYNAGNIYSVDYALKRLGVEATITSDKEVLMSADKVIFPGVGEAETTMSHLRKN
CEEEEEEECCCCEEEHHHHHHHCCCEEEECCCHHHHHCCCCEEECCCCCHHHHHHHHHHH
RLDEVIKNLKQPVLGICLGMQLMCRHSEEGNADCLGIFDTDVKLFSPTRHEDKVPHMGWN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCHH
TLTHVRSDLFKGFTKEEFVYFVHSFYVPLNEFTAAQTDYILPYSSALHKDNFYATQFHPE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCEECCCHHHHCCCCCEEEEECCC
KSGAVGERILRNFLEL
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIAVVKYNAGNIYSVDYALKRLGVEATITSDKEVLMSADKVIFPGVGEAETTMSHLRKN
CEEEEEEECCCCEEEHHHHHHHCCCEEEECCCHHHHHCCCCEEECCCCCHHHHHHHHHHH
RLDEVIKNLKQPVLGICLGMQLMCRHSEEGNADCLGIFDTDVKLFSPTRHEDKVPHMGWN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCHH
TLTHVRSDLFKGFTKEEFVYFVHSFYVPLNEFTAAQTDYILPYSSALHKDNFYATQFHPE
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCEECCCHHHHCCCCCEEEEECCC
KSGAVGERILRNFLEL
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA