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The map label for this gene is purU [H]

Identifier: 150006331

GI number: 150006331

Start: 4751477

End: 4752334

Strand: Direct

Name: purU [H]

Synonym: BVU_3850

Alternate gene names: 150006331

Gene position: 4751477-4752334 (Clockwise)

Preceding gene: 150006327

Following gene: 150006332

Centisome position: 92.03

GC content: 38.0

Gene sequence:

>858_bases
ATGAATACAACTGCAAAATTATTGTTGCACTGCCCAGACCAACCAGGCATTTTGGCAGAAGTCACAAACTTTATTACAAT
TAACAAAGGTAATATTGTCTACTTAGACCAATATGTGGACCATGTAGAAAATATATTTTTCATGCGTATAGAATGGGAAA
TAAAGGATTTCTTTATTCCTAAAAATAAAATAGAAGATTATTTTGAAACCTTGTATGCCCAAAAATATGAAATGAAATTC
CGTCTTTATTTCTCGGATGTAAAACCCAAAATGGCCATATTTGTTTCCAAACTGTCCCATTGCCTGTTCGATATGCTGGC
ACGCTACACGGCCGGAGAATGGAATGTGGAAATTCCGCTGATTATAAGTAACCATCCCGACCTTCAGCATGTGGCAGAAC
GTTTCGGTATACCGTTCTATCTCTTCCCTATTACTAAAGAGAACAAAATGGAACAGGAAAAAGCAGAAATGGAGCTATTG
GAAAAACATAACATCAATTTCATTGTACTGGCACGCTATATGCAAGTGATTTCCGAACAAATGATTGAAGCATATCCCAA
CCGCATCATCAACATACACCACTCTTTCCTCCCTGCGTTCGTTGGTGCAAAACCTTATCATGCAGCGTTTGAACGAGGAG
TAAAAATCATTGGTGCGACCAGCCATTATGTTACCTCCGAACTGGATGCCGGTCCTATCATTGAGCAAGATGTAGTACGC
ATCACACACAAGGACACTGTGCAGGATTTAGTAAGTAAAGGTAAAGATTTGGAAAAAATAGTCCTTTCTCGTGCTGTTCA
GAAACATATTGAACGAAAAGTATTGGCTTATAAAAATAAAACAGTTATCTTTAGCTGA

Upstream 100 bases:

>100_bases
AATCTTTTCAACAAACTTCTTGTTATAAAATTTAGCTTATTGGCAATAATTCGCTATTTTTGTGCACTCAATAGATTAAA
ATCGAAAATTAGGGAAAAAG

Downstream 100 bases:

>100_bases
TGAAAATTGCTGTCGTAAAATATAATGCAGGAAACATCTACTCCGTAGACTATGCACTGAAGCGCCTTGGAGTAGAAGCA
ACCATCACTTCCGATAAGGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNTTAKLLLHCPDQPGILAEVTNFITINKGNIVYLDQYVDHVENIFFMRIEWEIKDFFIPKNKIEDYFETLYAQKYEMKF
RLYFSDVKPKMAIFVSKLSHCLFDMLARYTAGEWNVEIPLIISNHPDLQHVAERFGIPFYLFPITKENKMEQEKAEMELL
EKHNINFIVLARYMQVISEQMIEAYPNRIINIHHSFLPAFVGAKPYHAAFERGVKIIGATSHYVTSELDAGPIIEQDVVR
ITHKDTVQDLVSKGKDLEKIVLSRAVQKHIERKVLAYKNKTVIFS

Sequences:

>Translated_285_residues
MNTTAKLLLHCPDQPGILAEVTNFITINKGNIVYLDQYVDHVENIFFMRIEWEIKDFFIPKNKIEDYFETLYAQKYEMKF
RLYFSDVKPKMAIFVSKLSHCLFDMLARYTAGEWNVEIPLIISNHPDLQHVAERFGIPFYLFPITKENKMEQEKAEMELL
EKHNINFIVLARYMQVISEQMIEAYPNRIINIHHSFLPAFVGAKPYHAAFERGVKIIGATSHYVTSELDAGPIIEQDVVR
ITHKDTVQDLVSKGKDLEKIVLSRAVQKHIERKVLAYKNKTVIFS
>Mature_285_residues
MNTTAKLLLHCPDQPGILAEVTNFITINKGNIVYLDQYVDHVENIFFMRIEWEIKDFFIPKNKIEDYFETLYAQKYEMKF
RLYFSDVKPKMAIFVSKLSHCLFDMLARYTAGEWNVEIPLIISNHPDLQHVAERFGIPFYLFPITKENKMEQEKAEMELL
EKHNINFIVLARYMQVISEQMIEAYPNRIINIHHSFLPAFVGAKPYHAAFERGVKIIGATSHYVTSELDAGPIIEQDVVR
ITHKDTVQDLVSKGKDLEKIVLSRAVQKHIERKVLAYKNKTVIFS

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=216, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI209869995, Length=216, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI209869993, Length=216, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1787483, Length=274, Percent_Identity=41.970802919708, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1788846, Length=88, Percent_Identity=35.2272727272727, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=166, Percent_Identity=30.7228915662651, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24582400, Length=206, Percent_Identity=27.6699029126214, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33272; Mature: 33272

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTAKLLLHCPDQPGILAEVTNFITINKGNIVYLDQYVDHVENIFFMRIEWEIKDFFIP
CCCCEEEEEECCCCCCHHHHHHCEEEECCCCEEEHHHHHHHHCCEEEEEEEEEEEEEECC
KNKIEDYFETLYAQKYEMKFRLYFSDVKPKMAIFVSKLSHCLFDMLARYTAGEWNVEIPL
HHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
IISNHPDLQHVAERFGIPFYLFPITKENKMEQEKAEMELLEKHNINFIVLARYMQVISEQ
EECCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
MIEAYPNRIINIHHSFLPAFVGAKPYHAAFERGVKIIGATSHYVTSELDAGPIIEQDVVR
HHHHCCHHEEEHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHHHHCCCCCCCCCHHHHHH
ITHKDTVQDLVSKGKDLEKIVLSRAVQKHIERKVLAYKNKTVIFS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MNTTAKLLLHCPDQPGILAEVTNFITINKGNIVYLDQYVDHVENIFFMRIEWEIKDFFIP
CCCCEEEEEECCCCCCHHHHHHCEEEECCCCEEEHHHHHHHHCCEEEEEEEEEEEEEECC
KNKIEDYFETLYAQKYEMKFRLYFSDVKPKMAIFVSKLSHCLFDMLARYTAGEWNVEIPL
HHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
IISNHPDLQHVAERFGIPFYLFPITKENKMEQEKAEMELLEKHNINFIVLARYMQVISEQ
EECCCCCHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
MIEAYPNRIINIHHSFLPAFVGAKPYHAAFERGVKIIGATSHYVTSELDAGPIIEQDVVR
HHHHCCHHEEEHHHHHHHHHHCCCCHHHHHHCCCEEEECCHHHHHCCCCCCCCCHHHHHH
ITHKDTVQDLVSKGKDLEKIVLSRAVQKHIERKVLAYKNKTVIFS
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]