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The map label for this gene is hsdM [H]

Identifier: 150006176

GI number: 150006176

Start: 4558051

End: 4559604

Strand: Reverse

Name: hsdM [H]

Synonym: BVU_3687

Alternate gene names: 150006176

Gene position: 4559604-4558051 (Counterclockwise)

Preceding gene: 150006177

Following gene: 150006175

Centisome position: 88.31

GC content: 40.86

Gene sequence:

>1554_bases
ATGAGCGAAGAATTACAGCAGAAACTCCGTGACCAACTTTGGGAGGTTGCAAATCGTTTGCGTGGCAATATGTCGGCAAG
TGACTTTATGTATTTTACATTAGGTTTCATCTTCTATAAGTACTTGTCGGAAAAAATAGAGAAGTATGCCAACAATGCCT
TGGTGGATGATGAAATAACCTTTAAGGAATTGTGGAAAATGGATGATGCCGATGCGGTGGAACTTCAAGAGGAAGCAAAG
AACCAATGCTTGGAGAATATCGGCTATTTCATAGAACCGAAATTCTTGTTTTCGTCTGTAATAGAGGCTATCAAGCGTAA
AGAAAATATCTTGCCTATGCTTGAACGTTCATTGAAGCGTATTGAAGACAGTACTTTAGGACAGGATAGTGAAGAAGATT
TTGGCGGATTGTTTTCAGATATAGACTTGGCATCACCTAAATTGGGCAAGACTGCCGACGATAAAAACACGCTCGTTAGT
AACGTGCTTCTTGCATTAGATGATATAGACTTCGGAGTAGAGGCATCACAGGAGATTGATATTTTGGGTGATGCTTACGA
ATATATGATTTCGCAATTTGCCGCAGGAGCCGGAAAAAAGGCTGGAGAGTTCTATACTCCGCAGGAAGTCAGCCGTATCT
TAGCAGAAATTGTTTCCATCGGACACCAGCGTTTACGCAATGTATATGACCCTACTTGCGGTAGTGGTTCGTTGCTTCTT
CGTGCTGCTAATATTGGAAATGCAGTTGATATTTATGGACAGGAAAAGAATCCTACAACTTATAACCTTGCCCGAATGAA
TATGTTGCTTCATGGCATCAGATTCAGCAACTTCAAGATTGAAAATGGTGATACACTTGAATGGGATGCTTTTGGTGATA
CTCAGTTTGATGCAGTGGTTGCAAATCCTCCGTTCTCAGCCGAATGGAGTGCTGCCGACAAATTCAATACTGATGATCGT
TTCAGCAAAGCAGGACGGCTTGCACCACGTAAGACTGCCGATTATGCATTTATTCTGCACATGATTTATCACTTGAACGA
GGGTGGTACAATGGCTTGTGTTGCTCCGCATGGTGTACTATTTCGAGGAAATGCGGAGGGAGTTATCCGTCGTTTCCTTA
TAGAAAAGAAAAACTATGTGGATGCCATTATAGGATTACCTGCGAATATTTTCTATGGTACAAGTATTCCGACTTGTATT
CTTGTTTTCAAGAAATGTCGTAAGGAAGATGACAATATTCTGTTTATTGATGCAAGCAAGGAGTTTGAAAAGGTAAAGAC
CCAGAACAAACTTCGTGAGCAACATATCCAAAAGATTGTCGAAACTTATCGTGACCGCAAGGAAATAGAAAAGTACAGTC
ACCTTGCCTCTCTTCAAGAAATAGCCGAGAATGATTACAATCTGAATATTCCACGTTACGTTGATACCTTTGAAGAGGAA
GAACCTATCGACATAAAGGCTGTAATGGCAGAGATAAAGGAGTTGGAAGCCAAACGTGCTGAACTCAACAAAGAGATTGA
AGTGTACCTGAAAGAATTGGGACTGGTAGAATAG

Upstream 100 bases:

>100_bases
AGAGAAGCATCTCGGTCTTATCAAAACCCGAAAGGCTTTGACGAGAATAATGGAAAGATTGCGTAGTATTATAAAAACGT
TTAATTGGGAATAAGATATT

Downstream 100 bases:

>100_bases
TTCAGAAAAGTAATAGCAATGGGTGAAAATGACAACATACCTCAGATGACTCCTTCATTTGAGAATATATGCCGCAAGGA
TGAAAACGGGAATGAATACT

Product: type I restriction enzyme EcoR124II M protein

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSEELQQKLRDQLWEVANRLRGNMSASDFMYFTLGFIFYKYLSEKIEKYANNALVDDEITFKELWKMDDADAVELQEEAK
NQCLENIGYFIEPKFLFSSVIEAIKRKENILPMLERSLKRIEDSTLGQDSEEDFGGLFSDIDLASPKLGKTADDKNTLVS
NVLLALDDIDFGVEASQEIDILGDAYEYMISQFAAGAGKKAGEFYTPQEVSRILAEIVSIGHQRLRNVYDPTCGSGSLLL
RAANIGNAVDIYGQEKNPTTYNLARMNMLLHGIRFSNFKIENGDTLEWDAFGDTQFDAVVANPPFSAEWSAADKFNTDDR
FSKAGRLAPRKTADYAFILHMIYHLNEGGTMACVAPHGVLFRGNAEGVIRRFLIEKKNYVDAIIGLPANIFYGTSIPTCI
LVFKKCRKEDDNILFIDASKEFEKVKTQNKLREQHIQKIVETYRDRKEIEKYSHLASLQEIAENDYNLNIPRYVDTFEEE
EPIDIKAVMAEIKELEAKRAELNKEIEVYLKELGLVE

Sequences:

>Translated_517_residues
MSEELQQKLRDQLWEVANRLRGNMSASDFMYFTLGFIFYKYLSEKIEKYANNALVDDEITFKELWKMDDADAVELQEEAK
NQCLENIGYFIEPKFLFSSVIEAIKRKENILPMLERSLKRIEDSTLGQDSEEDFGGLFSDIDLASPKLGKTADDKNTLVS
NVLLALDDIDFGVEASQEIDILGDAYEYMISQFAAGAGKKAGEFYTPQEVSRILAEIVSIGHQRLRNVYDPTCGSGSLLL
RAANIGNAVDIYGQEKNPTTYNLARMNMLLHGIRFSNFKIENGDTLEWDAFGDTQFDAVVANPPFSAEWSAADKFNTDDR
FSKAGRLAPRKTADYAFILHMIYHLNEGGTMACVAPHGVLFRGNAEGVIRRFLIEKKNYVDAIIGLPANIFYGTSIPTCI
LVFKKCRKEDDNILFIDASKEFEKVKTQNKLREQHIQKIVETYRDRKEIEKYSHLASLQEIAENDYNLNIPRYVDTFEEE
EPIDIKAVMAEIKELEAKRAELNKEIEVYLKELGLVE
>Mature_516_residues
SEELQQKLRDQLWEVANRLRGNMSASDFMYFTLGFIFYKYLSEKIEKYANNALVDDEITFKELWKMDDADAVELQEEAKN
QCLENIGYFIEPKFLFSSVIEAIKRKENILPMLERSLKRIEDSTLGQDSEEDFGGLFSDIDLASPKLGKTADDKNTLVSN
VLLALDDIDFGVEASQEIDILGDAYEYMISQFAAGAGKKAGEFYTPQEVSRILAEIVSIGHQRLRNVYDPTCGSGSLLLR
AANIGNAVDIYGQEKNPTTYNLARMNMLLHGIRFSNFKIENGDTLEWDAFGDTQFDAVVANPPFSAEWSAADKFNTDDRF
SKAGRLAPRKTADYAFILHMIYHLNEGGTMACVAPHGVLFRGNAEGVIRRFLIEKKNYVDAIIGLPANIFYGTSIPTCIL
VFKKCRKEDDNILFIDASKEFEKVKTQNKLREQHIQKIVETYRDRKEIEKYSHLASLQEIAENDYNLNIPRYVDTFEEEE
PIDIKAVMAEIKELEAKRAELNKEIEVYLKELGLVE

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=356, Percent_Identity=26.685393258427, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 58907; Mature: 58776

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEELQQKLRDQLWEVANRLRGNMSASDFMYFTLGFIFYKYLSEKIEKYANNALVDDEIT
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FKELWKMDDADAVELQEEAKNQCLENIGYFIEPKFLFSSVIEAIKRKENILPMLERSLKR
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHHH
IEDSTLGQDSEEDFGGLFSDIDLASPKLGKTADDKNTLVSNVLLALDDIDFGVEASQEID
HHHHHCCCCCCHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
ILGDAYEYMISQFAAGAGKKAGEFYTPQEVSRILAEIVSIGHQRLRNVYDPTCGSGSLLL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
RAANIGNAVDIYGQEKNPTTYNLARMNMLLHGIRFSNFKIENGDTLEWDAFGDTQFDAVV
EECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCEEEECCCCCCCCCEEE
ANPPFSAEWSAADKFNTDDRFSKAGRLAPRKTADYAFILHMIYHLNEGGTMACVAPHGVL
ECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEE
FRGNAEGVIRRFLIEKKNYVDAIIGLPANIFYGTSIPTCILVFKKCRKEDDNILFIDASK
EECCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCH
EFEKVKTQNKLREQHIQKIVETYRDRKEIEKYSHLASLQEIAENDYNLNIPRYVDTFEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCC
EPIDIKAVMAEIKELEAKRAELNKEIEVYLKELGLVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEELQQKLRDQLWEVANRLRGNMSASDFMYFTLGFIFYKYLSEKIEKYANNALVDDEIT
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FKELWKMDDADAVELQEEAKNQCLENIGYFIEPKFLFSSVIEAIKRKENILPMLERSLKR
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHHH
IEDSTLGQDSEEDFGGLFSDIDLASPKLGKTADDKNTLVSNVLLALDDIDFGVEASQEID
HHHHHCCCCCCHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHH
ILGDAYEYMISQFAAGAGKKAGEFYTPQEVSRILAEIVSIGHQRLRNVYDPTCGSGSLLL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
RAANIGNAVDIYGQEKNPTTYNLARMNMLLHGIRFSNFKIENGDTLEWDAFGDTQFDAVV
EECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCEEECCCCEEEECCCCCCCCCEEE
ANPPFSAEWSAADKFNTDDRFSKAGRLAPRKTADYAFILHMIYHLNEGGTMACVAPHGVL
ECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCEE
FRGNAEGVIRRFLIEKKNYVDAIIGLPANIFYGTSIPTCILVFKKCRKEDDNILFIDASK
EECCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEECCH
EFEKVKTQNKLREQHIQKIVETYRDRKEIEKYSHLASLQEIAENDYNLNIPRYVDTFEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCC
EPIDIKAVMAEIKELEAKRAELNKEIEVYLKELGLVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]