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The map label for this gene is rlmI [H]

Identifier: 150006112

GI number: 150006112

Start: 4487286

End: 4488470

Strand: Reverse

Name: rlmI [H]

Synonym: BVU_3623

Alternate gene names: 150006112

Gene position: 4488470-4487286 (Counterclockwise)

Preceding gene: 150006113

Following gene: 150006108

Centisome position: 86.93

GC content: 45.91

Gene sequence:

>1185_bases
ATGGCTTATAAGAAAGTATACCTGAAACCGGGTAAGGAAGAGTCGTTAAAGCGTTTTCATCCGTGGGTATTCTCCGGAGC
TATCCAGCGCATTGAGGGAGAACCCGAAGAAGGCGAGATAGTGGATGTGTACACTTCGAAGAAAGAGTTTATAGCTTGCG
GGCATTTCCAGATAGGAAGTATTGCCGTGCGCGTGCTCTCTTTCAAGGAAGGTGAGATTGATCATGAATTTTGGAAGCAT
AAACTGGAAGTGGCATACGATTTGCGCCGCAGTTTGGGTTTGGCCGGTAATCCTGCAAACAATACTTACCGTCTGGTTCA
TGGCGAAGGAGACAACTTGCCGGGCTTGATTATTGATGTATATGATCATACAGCCGTGATGCAGGCACATTCGGCCGGAA
TGCATGTTTACCGTATGGAGATAGCCGATGCCTTGTCCGAGGTGATGGGGGATGTAGTCCGGAACATTTATTATAAATCA
GAAACCACATTGCCTTTCAAGGCGGATTTGGGACCGGAGAATGGTTTTATCAAGGGGGGAAGCTCCGAAAACATAGCGAT
GGAATACGGCTTGAAATTCCATGTGGATTGGCTGAAAGGTCAGAAAACAGGCTTTTTTGTGGATCAGCGTGAGAACCGCC
ATTTGTTGGAGCGTTATGCCAAAGGGCGTAATGTATTGAATATGTTCTGCTATACAGGAGGCTTCTCTTTCTATGCCATG
CGTGGAGGCGCGAATCTGGTACATTCGGTAGACAGTTCGGCAAAAGCTATTGATCTTACCAATATGAACGTGGAATTAAA
TTTTCCGGGTGATACCCGTCATAAGGCGTATGCTGAGGATGCTTTTAAATACCTGGATCGTATGGGGGATCAATATGATT
TGATAATTCTTGACCCTCCTGCATTTGCCAAACACAAGGATGCTCTCCGCAATGCATTGCGCGGCTATACGAAATTGAAC
GCTAAAGCATTTGAGAAAATCAAACCGGGAGGCATTCTGTTTACTTTCTCTTGTTCGCAGGTGGTGGATAAGGTGAATTT
CCGTAATGCGGTATTTACAGCTGCCGCACAAAGCGGACGTAGTGTGCGTATTCTGCATCAATTGACCCAGCCGGGAGATC
ATCCCGTAAATATTTACCATCCTGAAGGAGAGTATTTGAAAGGGCTGGTGCTGTATGTGGAATAA

Upstream 100 bases:

>100_bases
TGGGCTTGTGTGGAAATTTACAATTGCCTGACGGAATTGGAACGTACGGGCGGTTATGAGATAACAGAGGAAGAACCAAT
AGAAACAACAGTAAATACAA

Downstream 100 bases:

>100_bases
TTTGATTGGTTTGTATAATCATATATAGTACAAAGGCGTTTTTACAGAATTTGAAACTGTAAAAGCGTCTTTAAAATTTA
AATGATGTTAATTGAAAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAYKKVYLKPGKEESLKRFHPWVFSGAIQRIEGEPEEGEIVDVYTSKKEFIACGHFQIGSIAVRVLSFKEGEIDHEFWKH
KLEVAYDLRRSLGLAGNPANNTYRLVHGEGDNLPGLIIDVYDHTAVMQAHSAGMHVYRMEIADALSEVMGDVVRNIYYKS
ETTLPFKADLGPENGFIKGGSSENIAMEYGLKFHVDWLKGQKTGFFVDQRENRHLLERYAKGRNVLNMFCYTGGFSFYAM
RGGANLVHSVDSSAKAIDLTNMNVELNFPGDTRHKAYAEDAFKYLDRMGDQYDLIILDPPAFAKHKDALRNALRGYTKLN
AKAFEKIKPGGILFTFSCSQVVDKVNFRNAVFTAAAQSGRSVRILHQLTQPGDHPVNIYHPEGEYLKGLVLYVE

Sequences:

>Translated_394_residues
MAYKKVYLKPGKEESLKRFHPWVFSGAIQRIEGEPEEGEIVDVYTSKKEFIACGHFQIGSIAVRVLSFKEGEIDHEFWKH
KLEVAYDLRRSLGLAGNPANNTYRLVHGEGDNLPGLIIDVYDHTAVMQAHSAGMHVYRMEIADALSEVMGDVVRNIYYKS
ETTLPFKADLGPENGFIKGGSSENIAMEYGLKFHVDWLKGQKTGFFVDQRENRHLLERYAKGRNVLNMFCYTGGFSFYAM
RGGANLVHSVDSSAKAIDLTNMNVELNFPGDTRHKAYAEDAFKYLDRMGDQYDLIILDPPAFAKHKDALRNALRGYTKLN
AKAFEKIKPGGILFTFSCSQVVDKVNFRNAVFTAAAQSGRSVRILHQLTQPGDHPVNIYHPEGEYLKGLVLYVE
>Mature_393_residues
AYKKVYLKPGKEESLKRFHPWVFSGAIQRIEGEPEEGEIVDVYTSKKEFIACGHFQIGSIAVRVLSFKEGEIDHEFWKHK
LEVAYDLRRSLGLAGNPANNTYRLVHGEGDNLPGLIIDVYDHTAVMQAHSAGMHVYRMEIADALSEVMGDVVRNIYYKSE
TTLPFKADLGPENGFIKGGSSENIAMEYGLKFHVDWLKGQKTGFFVDQRENRHLLERYAKGRNVLNMFCYTGGFSFYAMR
GGANLVHSVDSSAKAIDLTNMNVELNFPGDTRHKAYAEDAFKYLDRMGDQYDLIILDPPAFAKHKDALRNALRGYTKLNA
KAFEKIKPGGILFTFSCSQVVDKVNFRNAVFTAAAQSGRSVRILHQLTQPGDHPVNIYHPEGEYLKGLVLYVE

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=397, Percent_Identity=37.7833753148615, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1787181, Length=194, Percent_Identity=27.319587628866, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 44447; Mature: 44316

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKKVYLKPGKEESLKRFHPWVFSGAIQRIEGEPEEGEIVDVYTSKKEFIACGHFQIGS
CCCEEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCEEHHH
IAVRVLSFKEGEIDHEFWKHKLEVAYDLRRSLGLAGNPANNTYRLVHGEGDNLPGLIIDV
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEE
YDHTAVMQAHSAGMHVYRMEIADALSEVMGDVVRNIYYKSETTLPFKADLGPENGFIKGG
CCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCEEECC
SSENIAMEYGLKFHVDWLKGQKTGFFVDQRENRHLLERYAKGRNVLNMFCYTGGFSFYAM
CCCCEEEEECCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEECCEEEEEE
RGGANLVHSVDSSAKAIDLTNMNVELNFPGDTRHKAYAEDAFKYLDRMGDQYDLIILDPP
ECCCCEEECCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
AFAKHKDALRNALRGYTKLNAKAFEKIKPGGILFTFSCSQVVDKVNFRNAVFTAAAQSGR
CCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHHHCCCHHHEEHHHHCCC
SVRILHQLTQPGDHPVNIYHPEGEYLKGLVLYVE
EEEEHHHHCCCCCCCEEEECCCCCCEEEEEEEEC
>Mature Secondary Structure 
AYKKVYLKPGKEESLKRFHPWVFSGAIQRIEGEPEEGEIVDVYTSKKEFIACGHFQIGS
CCEEEEECCCCHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCEEHHH
IAVRVLSFKEGEIDHEFWKHKLEVAYDLRRSLGLAGNPANNTYRLVHGEGDNLPGLIIDV
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEEEE
YDHTAVMQAHSAGMHVYRMEIADALSEVMGDVVRNIYYKSETTLPFKADLGPENGFIKGG
CCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCEEECC
SSENIAMEYGLKFHVDWLKGQKTGFFVDQRENRHLLERYAKGRNVLNMFCYTGGFSFYAM
CCCCEEEEECCEEEEEEECCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEECCEEEEEE
RGGANLVHSVDSSAKAIDLTNMNVELNFPGDTRHKAYAEDAFKYLDRMGDQYDLIILDPP
ECCCCEEECCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
AFAKHKDALRNALRGYTKLNAKAFEKIKPGGILFTFSCSQVVDKVNFRNAVFTAAAQSGR
CCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHHHHCCCHHHEEHHHHCCC
SVRILHQLTQPGDHPVNIYHPEGEYLKGLVLYVE
EEEEHHHHCCCCCCCEEEECCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA