Click here to switch to the map view.

The map label for this gene is potD-A [H]

Identifier: 150006049

GI number: 150006049

Start: 4406650

End: 4407984

Strand: Reverse

Name: potD-A [H]

Synonym: BVU_3553

Alternate gene names: 150006049

Gene position: 4407984-4406650 (Counterclockwise)

Preceding gene: 150006050

Following gene: 150006048

Centisome position: 85.37

GC content: 43.45

Gene sequence:

>1335_bases
ATGAGGAAAATAAATAGATGGCTGATGGTGTGTGTGTGCGCGTTATTCTCTTTAACCGGATGCTATAACACAGAGCCTCG
TGAGAGTGTGCTGAAAATATATAATTGGGCCGATTACATTGATGAGGATATGTTGGCGGAATTTCCCCGGTGGTACAAAG
AACAGACGGGGGAAGATATTCGGATTGTCTATCAGACTTTTGACATCAATGAAATCATGTTGACTAAGATAGAACGCGGG
CATGAAGATTTTGATGTAGTTTGTCCTTCGGAGTATATCATAGAGCGCATGCTGAGGAAAGGTTTATTGCTTCCCATTGA
TACCGTTTTTGGAAAGACCCCCAATTATCTGCATAATGAATCTCCTTATATCCGTGAACAATTGGATAAATTGAGCTGGT
CGGGGCATCGTGCTTCAGATTATGCCATCGGTTATATGTGGGGAACAGCAGGTATCCTTTTTAATACCGATCATGTTCCA
GCGGAAGATGCCAGGACTTGGGCCAGTCTTTGGAATCTGAAATATGAGAACAAGATCCTGATGAAGGAAAGTTATCGTGA
TGCATATGGTACCGCATTGATTTATGCCAATGCCGAGGATTTGAAAAAAGGCAAGGTTACGGTAGAGGAGTTGATGAACA
ACTATTCGCAAGCATCCATCGATACAGTTGAAAAGTATCTGAAAGCCATGAAACCCTTGATAGCAGGTTGGGAAGCTGAT
TTTGGCAAGGAAATGATGACTAAAGGTAAAGCATGGCTGAACATGACCTGGAGCGGGGATGCGGTATGGGCGATAGAAGA
GGCTGAAGGAGTGGGCATCCATCTGGACTATGAGGTGCCGGTGGAAGGAAGTAATGTGTGGTATGACGGATGGGTGATCC
CTAAGTATGCTAGAAATAAAAAGGCTGCCAGCTATTTTATTAATTATTTATGCCGTCCCGATGTGGCTTTACGTAATATG
GACGCCTGTGGTTATGTGAGTGCCGTTGCTACTCCCGAGATTTTGGAGGCAAAAATAGATACTACTTTATCTTACTTTGC
AGATTTAAGCTATTTTTTCGGTCCTGCTGCCGATAGTATACAAATAGATAAGGTACAGTATCCTGACCGTACAGTGGTGG
AGCGTTGTGCCATGATTCGTGATTCCGGTGACAAGACCGAATTGGTCTTGGAAATGTGGTCGCGTGTAAAGGGGGATAAT
TTGGGGGTAGGCGTGGTTATTGTGATTCTGGTATCTGCCGGGATTATGGTAGTATTCACCGTGTATAGAAAACTTCAGCG
TTATCAGCATGAAAAGGCTCAATATCGTCGCAGGAATCATCAAAGAAAGAAATGA

Upstream 100 bases:

>100_bases
CCCTGAACTTCGTCCGCTTTCTACCATTATATTCGTATTGGTGCTGGTATTGCTGGTTATCATCAATCGCCGTGCAGGCA
AGAAAAAGGAGGTATAAAAA

Downstream 100 bases:

>100_bases
CTTTATAAAGGAAGGAAACATTTAGTCTTGCTGATAAAACGATTTAGGGAATCCAATCTTTAGGTAGAATTAAAATAGGG
ACATTCATTAAATATTACAA

Product: putative ABC transport system, lipoprotein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRKINRWLMVCVCALFSLTGCYNTEPRESVLKIYNWADYIDEDMLAEFPRWYKEQTGEDIRIVYQTFDINEIMLTKIERG
HEDFDVVCPSEYIIERMLRKGLLLPIDTVFGKTPNYLHNESPYIREQLDKLSWSGHRASDYAIGYMWGTAGILFNTDHVP
AEDARTWASLWNLKYENKILMKESYRDAYGTALIYANAEDLKKGKVTVEELMNNYSQASIDTVEKYLKAMKPLIAGWEAD
FGKEMMTKGKAWLNMTWSGDAVWAIEEAEGVGIHLDYEVPVEGSNVWYDGWVIPKYARNKKAASYFINYLCRPDVALRNM
DACGYVSAVATPEILEAKIDTTLSYFADLSYFFGPAADSIQIDKVQYPDRTVVERCAMIRDSGDKTELVLEMWSRVKGDN
LGVGVVIVILVSAGIMVVFTVYRKLQRYQHEKAQYRRRNHQRKK

Sequences:

>Translated_444_residues
MRKINRWLMVCVCALFSLTGCYNTEPRESVLKIYNWADYIDEDMLAEFPRWYKEQTGEDIRIVYQTFDINEIMLTKIERG
HEDFDVVCPSEYIIERMLRKGLLLPIDTVFGKTPNYLHNESPYIREQLDKLSWSGHRASDYAIGYMWGTAGILFNTDHVP
AEDARTWASLWNLKYENKILMKESYRDAYGTALIYANAEDLKKGKVTVEELMNNYSQASIDTVEKYLKAMKPLIAGWEAD
FGKEMMTKGKAWLNMTWSGDAVWAIEEAEGVGIHLDYEVPVEGSNVWYDGWVIPKYARNKKAASYFINYLCRPDVALRNM
DACGYVSAVATPEILEAKIDTTLSYFADLSYFFGPAADSIQIDKVQYPDRTVVERCAMIRDSGDKTELVLEMWSRVKGDN
LGVGVVIVILVSAGIMVVFTVYRKLQRYQHEKAQYRRRNHQRKK
>Mature_444_residues
MRKINRWLMVCVCALFSLTGCYNTEPRESVLKIYNWADYIDEDMLAEFPRWYKEQTGEDIRIVYQTFDINEIMLTKIERG
HEDFDVVCPSEYIIERMLRKGLLLPIDTVFGKTPNYLHNESPYIREQLDKLSWSGHRASDYAIGYMWGTAGILFNTDHVP
AEDARTWASLWNLKYENKILMKESYRDAYGTALIYANAEDLKKGKVTVEELMNNYSQASIDTVEKYLKAMKPLIAGWEAD
FGKEMMTKGKAWLNMTWSGDAVWAIEEAEGVGIHLDYEVPVEGSNVWYDGWVIPKYARNKKAASYFINYLCRPDVALRNM
DACGYVSAVATPEILEAKIDTTLSYFADLSYFFGPAADSIQIDKVQYPDRTVVERCAMIRDSGDKTELVLEMWSRVKGDN
LGVGVVIVILVSAGIMVVFTVYRKLQRYQHEKAQYRRRNHQRKK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=376, Percent_Identity=26.3297872340426, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1787078, Length=349, Percent_Identity=29.7994269340974, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 51335; Mature: 51335

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKINRWLMVCVCALFSLTGCYNTEPRESVLKIYNWADYIDEDMLAEFPRWYKEQTGEDI
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCE
RIVYQTFDINEIMLTKIERGHEDFDVVCPSEYIIERMLRKGLLLPIDTVFGKTPNYLHNE
EEEEEECCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCC
SPYIREQLDKLSWSGHRASDYAIGYMWGTAGILFNTDHVPAEDARTWASLWNLKYENKIL
CCHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCCCCEEE
MKESYRDAYGTALIYANAEDLKKGKVTVEELMNNYSQASIDTVEKYLKAMKPLIAGWEAD
EHHHCCHHHCCEEEEECHHHHHCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
FGKEMMTKGKAWLNMTWSGDAVWAIEEAEGVGIHLDYEVPVEGSNVWYDGWVIPKYARNK
HHHHHHHCCCEEEEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEECCEECCHHHCCH
KAASYFINYLCRPDVALRNMDACGYVSAVATPEILEAKIDTTLSYFADLSYFFGPAADSI
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
QIDKVQYPDRTVVERCAMIRDSGDKTELVLEMWSRVKGDNLGVGVVIVILVSAGIMVVFT
EEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VYRKLQRYQHEKAQYRRRNHQRKK
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRKINRWLMVCVCALFSLTGCYNTEPRESVLKIYNWADYIDEDMLAEFPRWYKEQTGEDI
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCE
RIVYQTFDINEIMLTKIERGHEDFDVVCPSEYIIERMLRKGLLLPIDTVFGKTPNYLHNE
EEEEEECCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCEEHHHHCCCCCCCCCC
SPYIREQLDKLSWSGHRASDYAIGYMWGTAGILFNTDHVPAEDARTWASLWNLKYENKIL
CCHHHHHHHHHCCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHHCCCCCCEEE
MKESYRDAYGTALIYANAEDLKKGKVTVEELMNNYSQASIDTVEKYLKAMKPLIAGWEAD
EHHHCCHHHCCEEEEECHHHHHCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCH
FGKEMMTKGKAWLNMTWSGDAVWAIEEAEGVGIHLDYEVPVEGSNVWYDGWVIPKYARNK
HHHHHHHCCCEEEEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEECCEECCHHHCCH
KAASYFINYLCRPDVALRNMDACGYVSAVATPEILEAKIDTTLSYFADLSYFFGPAADSI
HHHHHHHHHHCCCCHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
QIDKVQYPDRTVVERCAMIRDSGDKTELVLEMWSRVKGDNLGVGVVIVILVSAGIMVVFT
EEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
VYRKLQRYQHEKAQYRRRNHQRKK
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]