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The map label for this gene is traC [H]

Identifier: 150005982

GI number: 150005982

Start: 4332097

End: 4333131

Strand: Reverse

Name: traC [H]

Synonym: BVU_3480

Alternate gene names: 150005982

Gene position: 4333131-4332097 (Counterclockwise)

Preceding gene: 150005983

Following gene: 150005975

Centisome position: 83.92

GC content: 50.43

Gene sequence:

>1035_bases
ATGAACGGGAAGGTACTGGAAAGATATGCGGAACTGATGATTGACAAGATCAGGCAGATGTCCGCCGGTGAATGGCAGAA
GCCCTGGTTCACCCCCCGTGCCGGCCTGCCACAGAACATATCAGGGCGTCCGTACAACAGCATGAACCGTCTTATGCTCT
ACATGGAGATGGACAGGATGGGATACACCCTCCCGGTATTCATGACCTTCCGTCAGCTGAAGGATGAGAACCTGATGGTC
ACAAAAGGATCGCACGCCCTTCCCGTGACTTTTTACGATATCACCGTGAAGCATAAGACGACGGGAGAAAAAATCAGCTT
CGATGATTACAAAAGCCTCCCGGAACTTCAGAAGCAGGAATATAAGGTGACTCCCTTCATGAAACATTTCTATGTATTCA
ACATAGACCAGACGGATTTCAAGGAAAAATATCCGGAGCGGTATGAGGGCATGAGGGTCAGATTCTCCGGTCCTGCCGTA
GCGGACAACGTAAAAGGAAATAGGAACCCCTGGCTGGATAAAATGATAAAGGAACAGAAATGGCTCTGTCCCATAGAACT
GAAGGTGCAGGACAGAGCATATTACAGCCCGTCCAAAGACAGGATCGTTCTTCCGGCTCCGGGACAATTCAAGGATATGG
AATCATTCCAGATGACCGCCCTTCACGAAATGGCCTACTCCACCGGGCATTCCTCACGACTGGACCGCGGCCTGCATCAC
CCGTTCGGAACGTGCGGATATGCCCGGGAGGAAATCATCGCCGAATTCACAGCTGCCGTCACAGGCAGGGATCTGGGAAT
AGCCGTAACTCCGAGAAAGGAGAACGCACAGTATCTGAAAAGCTGGCTGTCAAATTTGAAGGAGGACCCCGGCTATGTCA
TGAGTGTGTTACGGGAGGCCGGCAAGGCATCCGCCATGATAGAGGAAAGTATGGAAAAACAGCACACCCTGGCAGACACA
TCCGCCAGGGAAGAGGACATATCCCCACTTCCCATAAAAGAATATAATGAAAGTGCCATTCATAATACTTTATGA

Upstream 100 bases:

>100_bases
CGGACTATGCCCGGGGCAATGGCATCACCTGTCCCACAGCAGCGGATGCGGTGCGTTTTTTGAAGGAATACGAGGAAAGG
CTTTATCAGGAACTGCTGCC

Downstream 100 bases:

>100_bases
ATGGCACTTTTTTATTGTTTTCCGGCAAGACAAAACCGTTATATCTTCATTCCCCTGCTTTTCCTTTGCGGCTGTATAGG
TCGGTGTATGCTCTGCCGTA

Product: DNA primase TraC

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNGKVLERYAELMIDKIRQMSAGEWQKPWFTPRAGLPQNISGRPYNSMNRLMLYMEMDRMGYTLPVFMTFRQLKDENLMV
TKGSHALPVTFYDITVKHKTTGEKISFDDYKSLPELQKQEYKVTPFMKHFYVFNIDQTDFKEKYPERYEGMRVRFSGPAV
ADNVKGNRNPWLDKMIKEQKWLCPIELKVQDRAYYSPSKDRIVLPAPGQFKDMESFQMTALHEMAYSTGHSSRLDRGLHH
PFGTCGYAREEIIAEFTAAVTGRDLGIAVTPRKENAQYLKSWLSNLKEDPGYVMSVLREAGKASAMIEESMEKQHTLADT
SAREEDISPLPIKEYNESAIHNTL

Sequences:

>Translated_344_residues
MNGKVLERYAELMIDKIRQMSAGEWQKPWFTPRAGLPQNISGRPYNSMNRLMLYMEMDRMGYTLPVFMTFRQLKDENLMV
TKGSHALPVTFYDITVKHKTTGEKISFDDYKSLPELQKQEYKVTPFMKHFYVFNIDQTDFKEKYPERYEGMRVRFSGPAV
ADNVKGNRNPWLDKMIKEQKWLCPIELKVQDRAYYSPSKDRIVLPAPGQFKDMESFQMTALHEMAYSTGHSSRLDRGLHH
PFGTCGYAREEIIAEFTAAVTGRDLGIAVTPRKENAQYLKSWLSNLKEDPGYVMSVLREAGKASAMIEESMEKQHTLADT
SAREEDISPLPIKEYNESAIHNTL
>Mature_344_residues
MNGKVLERYAELMIDKIRQMSAGEWQKPWFTPRAGLPQNISGRPYNSMNRLMLYMEMDRMGYTLPVFMTFRQLKDENLMV
TKGSHALPVTFYDITVKHKTTGEKISFDDYKSLPELQKQEYKVTPFMKHFYVFNIDQTDFKEKYPERYEGMRVRFSGPAV
ADNVKGNRNPWLDKMIKEQKWLCPIELKVQDRAYYSPSKDRIVLPAPGQFKDMESFQMTALHEMAYSTGHSSRLDRGLHH
PFGTCGYAREEIIAEFTAAVTGRDLGIAVTPRKENAQYLKSWLSNLKEDPGYVMSVLREAGKASAMIEESMEKQHTLADT
SAREEDISPLPIKEYNESAIHNTL

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 39816; Mature: 39816

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKVLERYAELMIDKIRQMSAGEWQKPWFTPRAGLPQNISGRPYNSMNRLMLYMEMDRM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEHHHC
GYTLPVFMTFRQLKDENLMVTKGSHALPVTFYDITVKHKTTGEKISFDDYKSLPELQKQE
CCCHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEECCCCCCCCCHHHHCCHHHHHHC
YKVTPFMKHFYVFNIDQTDFKEKYPERYEGMRVRFSGPAVADNVKGNRNPWLDKMIKEQK
CCCCCEEEEEEEEECCCHHHHHHCHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHC
WLCPIELKVQDRAYYSPSKDRIVLPAPGQFKDMESFQMTALHEMAYSTGHSSRLDRGLHH
CCCCEEEEECCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PFGTCGYAREEIIAEFTAAVTGRDLGIAVTPRKENAQYLKSWLSNLKEDPGYVMSVLREA
CCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
GKASAMIEESMEKQHTLADTSAREEDISPLPIKEYNESAIHNTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHCCC
>Mature Secondary Structure
MNGKVLERYAELMIDKIRQMSAGEWQKPWFTPRAGLPQNISGRPYNSMNRLMLYMEMDRM
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEHHHC
GYTLPVFMTFRQLKDENLMVTKGSHALPVTFYDITVKHKTTGEKISFDDYKSLPELQKQE
CCCHHHHHHHHHHCCCCEEEECCCCEEEEEEEEEEEEECCCCCCCCCHHHHCCHHHHHHC
YKVTPFMKHFYVFNIDQTDFKEKYPERYEGMRVRFSGPAVADNVKGNRNPWLDKMIKEQK
CCCCCEEEEEEEEECCCHHHHHHCHHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHC
WLCPIELKVQDRAYYSPSKDRIVLPAPGQFKDMESFQMTALHEMAYSTGHSSRLDRGLHH
CCCCEEEEECCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
PFGTCGYAREEIIAEFTAAVTGRDLGIAVTPRKENAQYLKSWLSNLKEDPGYVMSVLREA
CCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHH
GKASAMIEESMEKQHTLADTSAREEDISPLPIKEYNESAIHNTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]