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The map label for this gene is pycB [H]

Identifier: 150005850

GI number: 150005850

Start: 4180900

End: 4182675

Strand: Reverse

Name: pycB [H]

Synonym: BVU_3346

Alternate gene names: 150005850

Gene position: 4182675-4180900 (Counterclockwise)

Preceding gene: 150005851

Following gene: 150005849

Centisome position: 81.01

GC content: 42.4

Gene sequence:

>1776_bases
ATGAAAAAAGAAATCAAATTCAGTTTGGTCTACCGCGATATGTGGCAGTCATCAGGGAAGTACCAGCCCAGAGCTGATCA
GTTAGCCAAAATTGCTCCTCTAATCGTTGAAATGGGGTGTTTTGCCCGAGTCGAAACTAATGGAGGTGCTTTTGAACAAG
TGAACTTGCTATATGGTGAGAATCCTAACAAGGCAGTGCGTACATTTACCAAGCCTTTACGTGAGGCGGGTATTCAGACG
CATATGTTGGACCGTGGTTTAAATGCATTGCGAATGTATCCTGTTCCAGCTGATGTACGTCGGTTGATGTATAGGGTAAA
ACATGCGCAAGGAGTGGATATTACTCGTATTTTTTGTGGTTTGAATGAAGTTCGTAATATTATTCCTTCCATAAAATATG
CTAAAGAAGCGGGAATGATTCCTCAAGCCACTCTTTGCATCACTTATTCTCCTGTACATACGGTGGATTATTATTCTTAT
ATTGCCGACCAGTTAATAGAAGCGGGGGCACCCGAAATCTGTTTGAAAGATATGGCAGGTATCGGCCGTCCTGAAATGTT
GGGGCAGTTGGTGAGAAATATCAAAGATAAACATCCTGAAGTCATTGTACAATATCATGGACATTCCGGTCCTGGGTTAT
CTATGGCATCTATTTTGGAAGTTTGTGAGAATGGTGCGGATATCATTGATGTCGCTATGGAGCCGTTGTCATGGGGAAAA
GTGCATCCTGACGTAATTTCTGTACAGGCTATGTTGAAAAATGCAGGTTTTCAGGTACCTGAAATCAATATGAAGGCTTA
TATGAAAGCGCGTAGCATGACGCAGGAATTTATTGATGATTTTTTGGGATATTTTATTGATCCTACCAACAAACATATGT
CTTCCTTGCTGCTGGGATGTGGACTTCCCGGTGGCATGATGGGTTCAATGATGGCCGATCTGAAAGGGGTGCATTCGGGC
ATCAATCTGATTCTGAAAGGACAGGGAAAGGAACCTATGAGTTTGGATGATTTGGTAGTGATGCTGTTTGAAGAAGTGGA
ATACGTATGGCCGAAATTGGGATATCCACCTTTAGTAACACCGTTTAGCCAATATGTGAAGAATGTGGCTTTGATGAATG
TTATGCAATTGGTGAAGGGAGAGGAGAGATGGACGATGATTGATAACCATACATGGGATATGATTTTGGGAAAAAGTGGA
AAATTGCCGGGTGCGTTGGCTCCTGAAATAATCGAACTGGCGAAAAGTAAAGGCTACGAATTCACTGATGAAGATCCTCA
GGTGAACTATCCTGATGAGCTGGACAAGTACCGTAAGGAGATGGATGATAATGGATGGGAATATGGTGAGGATGAGGAAG
AGTTGTTTGAACTGGCTATGCATGACCGGCAATACCGTGATTATAAATCGGGTGTAGCCAAGAAACGTTTTATGGATGAT
TTACAACGTGCTCAGGATGCGGCTATGGTAAAAAGTGGCTTCGATGAGGAAACGATCCGAAAATATAAGCGTGCTAAGGC
AGATCCTATTATTGCACCGGATAAGGGGCAGATTTTATGGGAAGTTTCCGTGGAAGGTCCTTCTATGGCACCGTTTGTCG
GACGGAAATACCAGCATGATGAAGTGTTCTGTTATATTTCCACTCCGTGGGGAGAGTATGAAAAGATTTTTGCTAATTTT
ACAGGACGTATTGTAGAAGTTTGCGCCCAACAAGGAGCTGTAGTTAATAAAGGTGATGTTATAGCTTATTTGGAAAGAAC
AGAGTTTACTGCATGA

Upstream 100 bases:

>100_bases
TGTTTTTGGAAGCTAAAAAACAGCTTCGAATATTAACTTTATTTTTATTCATTTGTTATATTTGCAAAAGGACAAGACAC
CTTTAAATCAAGTAATAATT

Downstream 100 bases:

>100_bases
TTTAAAGGTGCTATATAGCTTTTTTTATCCGCTATGGCAAGTGTTGAATTTGTCATGGCGGATGTTTTTATCTTTTCATT
CTTTTAAAATTTGCATTCAT

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKKEIKFSLVYRDMWQSSGKYQPRADQLAKIAPLIVEMGCFARVETNGGAFEQVNLLYGENPNKAVRTFTKPLREAGIQT
HMLDRGLNALRMYPVPADVRRLMYRVKHAQGVDITRIFCGLNEVRNIIPSIKYAKEAGMIPQATLCITYSPVHTVDYYSY
IADQLIEAGAPEICLKDMAGIGRPEMLGQLVRNIKDKHPEVIVQYHGHSGPGLSMASILEVCENGADIIDVAMEPLSWGK
VHPDVISVQAMLKNAGFQVPEINMKAYMKARSMTQEFIDDFLGYFIDPTNKHMSSLLLGCGLPGGMMGSMMADLKGVHSG
INLILKGQGKEPMSLDDLVVMLFEEVEYVWPKLGYPPLVTPFSQYVKNVALMNVMQLVKGEERWTMIDNHTWDMILGKSG
KLPGALAPEIIELAKSKGYEFTDEDPQVNYPDELDKYRKEMDDNGWEYGEDEEELFELAMHDRQYRDYKSGVAKKRFMDD
LQRAQDAAMVKSGFDEETIRKYKRAKADPIIAPDKGQILWEVSVEGPSMAPFVGRKYQHDEVFCYISTPWGEYEKIFANF
TGRIVEVCAQQGAVVNKGDVIAYLERTEFTA

Sequences:

>Translated_591_residues
MKKEIKFSLVYRDMWQSSGKYQPRADQLAKIAPLIVEMGCFARVETNGGAFEQVNLLYGENPNKAVRTFTKPLREAGIQT
HMLDRGLNALRMYPVPADVRRLMYRVKHAQGVDITRIFCGLNEVRNIIPSIKYAKEAGMIPQATLCITYSPVHTVDYYSY
IADQLIEAGAPEICLKDMAGIGRPEMLGQLVRNIKDKHPEVIVQYHGHSGPGLSMASILEVCENGADIIDVAMEPLSWGK
VHPDVISVQAMLKNAGFQVPEINMKAYMKARSMTQEFIDDFLGYFIDPTNKHMSSLLLGCGLPGGMMGSMMADLKGVHSG
INLILKGQGKEPMSLDDLVVMLFEEVEYVWPKLGYPPLVTPFSQYVKNVALMNVMQLVKGEERWTMIDNHTWDMILGKSG
KLPGALAPEIIELAKSKGYEFTDEDPQVNYPDELDKYRKEMDDNGWEYGEDEEELFELAMHDRQYRDYKSGVAKKRFMDD
LQRAQDAAMVKSGFDEETIRKYKRAKADPIIAPDKGQILWEVSVEGPSMAPFVGRKYQHDEVFCYISTPWGEYEKIFANF
TGRIVEVCAQQGAVVNKGDVIAYLERTEFTA
>Mature_591_residues
MKKEIKFSLVYRDMWQSSGKYQPRADQLAKIAPLIVEMGCFARVETNGGAFEQVNLLYGENPNKAVRTFTKPLREAGIQT
HMLDRGLNALRMYPVPADVRRLMYRVKHAQGVDITRIFCGLNEVRNIIPSIKYAKEAGMIPQATLCITYSPVHTVDYYSY
IADQLIEAGAPEICLKDMAGIGRPEMLGQLVRNIKDKHPEVIVQYHGHSGPGLSMASILEVCENGADIIDVAMEPLSWGK
VHPDVISVQAMLKNAGFQVPEINMKAYMKARSMTQEFIDDFLGYFIDPTNKHMSSLLLGCGLPGGMMGSMMADLKGVHSG
INLILKGQGKEPMSLDDLVVMLFEEVEYVWPKLGYPPLVTPFSQYVKNVALMNVMQLVKGEERWTMIDNHTWDMILGKSG
KLPGALAPEIIELAKSKGYEFTDEDPQVNYPDELDKYRKEMDDNGWEYGEDEEELFELAMHDRQYRDYKSGVAKKRFMDD
LQRAQDAAMVKSGFDEETIRKYKRAKADPIIAPDKGQILWEVSVEGPSMAPFVGRKYQHDEVFCYISTPWGEYEKIFANF
TGRIVEVCAQQGAVVNKGDVIAYLERTEFTA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=254, Percent_Identity=28.740157480315, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI106049295, Length=254, Percent_Identity=28.740157480315, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI106049292, Length=254, Percent_Identity=28.740157480315, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17562816, Length=266, Percent_Identity=27.0676691729323, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6319695, Length=602, Percent_Identity=23.2558139534884, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321376, Length=603, Percent_Identity=22.8855721393035, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652212, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652210, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652214, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19921944, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652216, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281363050, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652224, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652222, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652220, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652218, Length=252, Percent_Identity=32.5396825396825, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 66736; Mature: 66736

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEIKFSLVYRDMWQSSGKYQPRADQLAKIAPLIVEMGCFARVETNGGAFEQVNLLYGE
CCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
NPNKAVRTFTKPLREAGIQTHMLDRGLNALRMYPVPADVRRLMYRVKHAQGVDITRIFCG
CCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHH
LNEVRNIIPSIKYAKEAGMIPQATLCITYSPVHTVDYYSYIADQLIEAGAPEICLKDMAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
IGRPEMLGQLVRNIKDKHPEVIVQYHGHSGPGLSMASILEVCENGADIIDVAMEPLSWGK
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEHHHHCCCCCCC
VHPDVISVQAMLKNAGFQVPEINMKAYMKARSMTQEFIDDFLGYFIDPTNKHMSSLLLGC
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GLPGGMMGSMMADLKGVHSGINLILKGQGKEPMSLDDLVVMLFEEVEYVWPKLGYPPLVT
CCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PFSQYVKNVALMNVMQLVKGEERWTMIDNHTWDMILGKSGKLPGALAPEIIELAKSKGYE
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCC
FTDEDPQVNYPDELDKYRKEMDDNGWEYGEDEEELFELAMHDRQYRDYKSGVAKKRFMDD
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRAQDAAMVKSGFDEETIRKYKRAKADPIIAPDKGQILWEVSVEGPSMAPFVGRKYQHD
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHCCCCCCC
EVFCYISTPWGEYEKIFANFTGRIVEVCAQQGAVVNKGDVIAYLERTEFTA
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCC
>Mature Secondary Structure
MKKEIKFSLVYRDMWQSSGKYQPRADQLAKIAPLIVEMGCFARVETNGGAFEQVNLLYGE
CCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEEECC
NPNKAVRTFTKPLREAGIQTHMLDRGLNALRMYPVPADVRRLMYRVKHAQGVDITRIFCG
CCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHH
LNEVRNIIPSIKYAKEAGMIPQATLCITYSPVHTVDYYSYIADQLIEAGAPEICLKDMAG
HHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
IGRPEMLGQLVRNIKDKHPEVIVQYHGHSGPGLSMASILEVCENGADIIDVAMEPLSWGK
CCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEHHHHCCCCCCC
VHPDVISVQAMLKNAGFQVPEINMKAYMKARSMTQEFIDDFLGYFIDPTNKHMSSLLLGC
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GLPGGMMGSMMADLKGVHSGINLILKGQGKEPMSLDDLVVMLFEEVEYVWPKLGYPPLVT
CCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
PFSQYVKNVALMNVMQLVKGEERWTMIDNHTWDMILGKSGKLPGALAPEIIELAKSKGYE
HHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCC
FTDEDPQVNYPDELDKYRKEMDDNGWEYGEDEEELFELAMHDRQYRDYKSGVAKKRFMDD
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRAQDAAMVKSGFDEETIRKYKRAKADPIIAPDKGQILWEVSVEGPSMAPFVGRKYQHD
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHCCCCCCC
EVFCYISTPWGEYEKIFANFTGRIVEVCAQQGAVVNKGDVIAYLERTEFTA
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]