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The map label for this gene is istB [H]

Identifier: 150005702

GI number: 150005702

Start: 4019634

End: 4020389

Strand: Reverse

Name: istB [H]

Synonym: BVU_3194

Alternate gene names: 150005702

Gene position: 4020389-4019634 (Counterclockwise)

Preceding gene: 150005703

Following gene: 150005697

Centisome position: 77.87

GC content: 42.72

Gene sequence:

>756_bases
ATGAAATCAGAAAAAGAAACCATTTATGACTATGCTGCAGAACTGAAGCTTCTGGCCTTTAAAGAGGAACTGGAATGCAC
CCTTTCATTGGCAGCTGAAGAAAACTGGAACCATCTGCAGTTCTTGACGGAATTGCTTGGAAAGGAAAGCGCCAGGAGAA
GGGAGTGTAGAAGAAGATCAAGGATAAGATCTGCGGGATTTCCACAAATGAAGTATCTGCATGAGCTTGTTATGGAAGAC
ATGCCCAAAGAGGCACAGGTAATATTACCTGAATTGGAGACACTGGACTTCATCAGACAGGGAAGAAACCTGGTCCTGTA
TGGAAATCCGGGAACGGGAAAGACGCATATTGCTACGGCTTTAGGAATAAAGGCCTGCCAACAGGACTTTACCGTATTGT
TTACTTCAGTGCCGGTCCTGCTTACCCAGATAAGGGAGGCTAAATCAGCAAAGACACTGAGGACGCTACAATTAAGGTTT
GAAAAATACGATCTGGTCATCTGTGATGAGTTCGGATATGTCAGTTGTGACAAGGAAGGAGGAGAACTGCTTTTTAACCA
CCTGTCGTTAAGAGCCGGAAAAAAGGCTACAATCATTACTACTAATTTGGCTTTTAACAGATGGAATGAAATCATAAAGG
ACAAGGTGCTTGTGGCGGCAATGGTTGACAGGCTTACACATAAAGCTTATCTGGTTAATATGACCGGACTGTCTTATAGG
CTTAAGGAAACACAAAAAATGAGACAAGATAAATGA

Upstream 100 bases:

>100_bases
TAGAAAGAGAAGCTGTGGATATGCTTGAAGGCATCACGGCGCTCATGACAGGATACAATGAAGTGCATGATATAATACCA
ACCATTTAAGTTTATAATAT

Downstream 100 bases:

>100_bases
ATATTTTAAACTTATAGTAATTTTGTAACAAGTTATGGATGGAGCTCTTTTCAATTAGAATACAGCGCACTTTTCAATTA
GTATCTACAATCCATATCCA

Product: putative transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKSEKETIYDYAAELKLLAFKEELECTLSLAAEENWNHLQFLTELLGKESARRRECRRRSRIRSAGFPQMKYLHELVMED
MPKEAQVILPELETLDFIRQGRNLVLYGNPGTGKTHIATALGIKACQQDFTVLFTSVPVLLTQIREAKSAKTLRTLQLRF
EKYDLVICDEFGYVSCDKEGGELLFNHLSLRAGKKATIITTNLAFNRWNEIIKDKVLVAAMVDRLTHKAYLVNMTGLSYR
LKETQKMRQDK

Sequences:

>Translated_251_residues
MKSEKETIYDYAAELKLLAFKEELECTLSLAAEENWNHLQFLTELLGKESARRRECRRRSRIRSAGFPQMKYLHELVMED
MPKEAQVILPELETLDFIRQGRNLVLYGNPGTGKTHIATALGIKACQQDFTVLFTSVPVLLTQIREAKSAKTLRTLQLRF
EKYDLVICDEFGYVSCDKEGGELLFNHLSLRAGKKATIITTNLAFNRWNEIIKDKVLVAAMVDRLTHKAYLVNMTGLSYR
LKETQKMRQDK
>Mature_251_residues
MKSEKETIYDYAAELKLLAFKEELECTLSLAAEENWNHLQFLTELLGKESARRRECRRRSRIRSAGFPQMKYLHELVMED
MPKEAQVILPELETLDFIRQGRNLVLYGNPGTGKTHIATALGIKACQQDFTVLFTSVPVLLTQIREAKSAKTLRTLQLRF
EKYDLVICDEFGYVSCDKEGGELLFNHLSLRAGKKATIITTNLAFNRWNEIIKDKVLVAAMVDRLTHKAYLVNMTGLSYR
LKETQKMRQDK

Specific function: One of two proteins expressed only when there is a tandem repeat of the IS21 insertion sequence, it is necessary for the transposition of plasmids with that tandem repeat [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS21/IS1162 putative ATP-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001270
- InterPro:   IPR002611 [H]

Pfam domain/function: PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 28939; Mature: 28939

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEKETIYDYAAELKLLAFKEELECTLSLAAEENWNHLQFLTELLGKESARRRECRRRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
RIRSAGFPQMKYLHELVMEDMPKEAQVILPELETLDFIRQGRNLVLYGNPGTGKTHIATA
HHHHCCCHHHHHHHHHHHHCCCCCHHEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
LGIKACQQDFTVLFTSVPVLLTQIREAKSAKTLRTLQLRFEKYDLVICDEFGYVSCDKEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCC
GELLFNHLSLRAGKKATIITTNLAFNRWNEIIKDKVLVAAMVDRLTHKAYLVNMTGLSYR
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHH
LKETQKMRQDK
HHHHHHHHCCC
>Mature Secondary Structure
MKSEKETIYDYAAELKLLAFKEELECTLSLAAEENWNHLQFLTELLGKESARRRECRRRS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
RIRSAGFPQMKYLHELVMEDMPKEAQVILPELETLDFIRQGRNLVLYGNPGTGKTHIATA
HHHHCCCHHHHHHHHHHHHCCCCCHHEECCHHHHHHHHHCCCCEEEECCCCCCHHHHHHH
LGIKACQQDFTVLFTSVPVLLTQIREAKSAKTLRTLQLRFEKYDLVICDEFGYVSCDKEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEECCCC
GELLFNHLSLRAGKKATIITTNLAFNRWNEIIKDKVLVAAMVDRLTHKAYLVNMTGLSYR
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHH
LKETQKMRQDK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2540414 [H]