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The map label for this gene is cbiF [H]

Identifier: 150005593

GI number: 150005593

Start: 3894762

End: 3896573

Strand: Reverse

Name: cbiF [H]

Synonym: BVU_3081

Alternate gene names: 150005593

Gene position: 3896573-3894762 (Counterclockwise)

Preceding gene: 150005594

Following gene: 150005592

Centisome position: 75.47

GC content: 48.45

Gene sequence:

>1812_bases
ATGAAAGCAATCATACTGATATCAGAAGCCAGCCTGCCGTTGGCAAAAACACTACAAAGAGAGTTGCCGGATACCCTTAT
TTATACCAAAAACGAATGCGAAGGCTGTATTTCCATCACATCCTGCCATCGGTTCATAGAGGAGCATTTCAATGACTTCG
ACAGCATCATCTTTATCGGTGCCATGGGAATCTGTGTACGCAGCATTGCAGGATGCATAAAAAACAAATATAAAGATCCG
GCAGTGGTCTGTGTGGACAGTACGGGGCGCTTTGTCATTTCAGTACTTTCCGGGCATGTGGGGGGAGCCAATGAACTGAC
CCGCCATATTGCCGCCATTACAGGAGGAGAAGCTGTGATTACCACCCAAAGTGACAATGCCGGGTTATGGGCGCTGGACA
CGCTGGCTGGAAAATATGGCTGGAAAATAACTGTTCCTCATGCAGAAATGAACAGACTGGTCACACTTTTTGTCAACCGG
GAACCAACGGCGCTGCTGCTCGATATAAAGGATAAAGGTACGGAGTATCTGGAACGCACGCTTCCCGCACACGTAAAAGT
GTTCTATCATTTCGAAGACATGCCCCAATCGGAATTCAAGTTGATTATTGCTGTTACACCTTATATATATAGTGCGGAGA
TTCCCATGCTCTGCTTCCATCCCGCCGTCCTCCACTTGGGAATAGGTTGCCGGAAACAATGCGATCCTTCGGGCATTGCG
GAATACATAGAAGCCGTGATGCACAGACATGGTTTGTGCCCGTTCTCTCTCGCCTCCCTCAATACCATCGAACTGAAAAA
AGACGAGCCTCTGCTGGAAATCCTCCACCGGCGATGGGCCGATACGGAAACCCATATTTATCCTGCGGAAGAACTGAAAG
ATATCACAGTTCCCCATCCGTCTGAAAAGGCATTCGAAGTGACAGGCGTCTATGGAGTGGCCGAAAGCACAGCCTTGAAA
AGCAGCGGTGAAGGAACACTTGTATTGGAAAAGCAAAAGGGCATGCTGACAGAAGGAAACCACTTCACTTTTGCCATAGC
CGTCAGTGCGACAGCCATGCGTGGCGGACATATAGAGATAGTGGGTGCAGGTCCCGGTGATCCCGAACTGATTTCGGTAC
GCGGAAAACGAATGTTGGAGAAAGCGGATCTTGTTCTGTATGCCGGAAGTCTTGTTCCCCGCGAACTGACCTTTTATGCT
AAAGAAGGAGCGACCGTACGCAGTTCCGCCGGTATGGATTTGGAGGAACAGTTCGCCTTGATGAAAGAGTTTTATGATAA
AGGATTGTTTATTGTACGCTTGCACACGGGAGACCCTTGCATTTATGGAGCCATCCAGGAACAAATGGCTTTCTTCGACC
GCTACAAGATGAGTTATCATATCACTCCGGGGATCTCCTCGTTCCAGGCGGCTGCGGCAGCATTGAGGTCACAGTTCACC
ATCCCCGAAAAAGTACAAAGCATAATCCTCACCCGTGGGGAAGGCCGTACTCCGATGCCCGAAAAAGAACAATTACATAA
ATTGGCGCAATCACAAAGTACAATGTGCATTTATTTAAGTGCGGGCATTGTGGAACAGGTACAGGAAGAGCTGATGCAGG
CATATCCTCCCGAGACTCCGGTTGCCGCCTGTTATAAGCTGACTTGGAAAGAAGAAAAGATTTATCGCGGAGAGTTGAAG
GATTTGGCTAAGATAGTACGTGACAATAACCTGACGCTGACTACCTTATTGGTAGTAGGCGAAGCGATAGACAACCGGAA
AGGTCTGTCCCGGCTCTATGCGCACGAGTTCAAACACTTGTTCAGAAAGTAA

Upstream 100 bases:

>100_bases
AATCAACAAGAAGGTAACACAGTGTACCCGTATTGCTGTGGATGCCTTTAATCCGATAGAGATAATGAGAGCGGAATAAA
TGATTTAAAACAGATAACAA

Downstream 100 bases:

>100_bases
AAAAAGAATCATTCACATCCAGCCTATGATACTGATTTTAGGAGGAACCACCGAAGGACGCACCGCCGTGAAAGTTGCCG
ATGAAGCCGGAAAGCCCTAC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 603; Mature: 603

Protein sequence:

>603_residues
MKAIILISEASLPLAKTLQRELPDTLIYTKNECEGCISITSCHRFIEEHFNDFDSIIFIGAMGICVRSIAGCIKNKYKDP
AVVCVDSTGRFVISVLSGHVGGANELTRHIAAITGGEAVITTQSDNAGLWALDTLAGKYGWKITVPHAEMNRLVTLFVNR
EPTALLLDIKDKGTEYLERTLPAHVKVFYHFEDMPQSEFKLIIAVTPYIYSAEIPMLCFHPAVLHLGIGCRKQCDPSGIA
EYIEAVMHRHGLCPFSLASLNTIELKKDEPLLEILHRRWADTETHIYPAEELKDITVPHPSEKAFEVTGVYGVAESTALK
SSGEGTLVLEKQKGMLTEGNHFTFAIAVSATAMRGGHIEIVGAGPGDPELISVRGKRMLEKADLVLYAGSLVPRELTFYA
KEGATVRSSAGMDLEEQFALMKEFYDKGLFIVRLHTGDPCIYGAIQEQMAFFDRYKMSYHITPGISSFQAAAAALRSQFT
IPEKVQSIILTRGEGRTPMPEKEQLHKLAQSQSTMCIYLSAGIVEQVQEELMQAYPPETPVAACYKLTWKEEKIYRGELK
DLAKIVRDNNLTLTTLLVVGEAIDNRKGLSRLYAHEFKHLFRK

Sequences:

>Translated_603_residues
MKAIILISEASLPLAKTLQRELPDTLIYTKNECEGCISITSCHRFIEEHFNDFDSIIFIGAMGICVRSIAGCIKNKYKDP
AVVCVDSTGRFVISVLSGHVGGANELTRHIAAITGGEAVITTQSDNAGLWALDTLAGKYGWKITVPHAEMNRLVTLFVNR
EPTALLLDIKDKGTEYLERTLPAHVKVFYHFEDMPQSEFKLIIAVTPYIYSAEIPMLCFHPAVLHLGIGCRKQCDPSGIA
EYIEAVMHRHGLCPFSLASLNTIELKKDEPLLEILHRRWADTETHIYPAEELKDITVPHPSEKAFEVTGVYGVAESTALK
SSGEGTLVLEKQKGMLTEGNHFTFAIAVSATAMRGGHIEIVGAGPGDPELISVRGKRMLEKADLVLYAGSLVPRELTFYA
KEGATVRSSAGMDLEEQFALMKEFYDKGLFIVRLHTGDPCIYGAIQEQMAFFDRYKMSYHITPGISSFQAAAAALRSQFT
IPEKVQSIILTRGEGRTPMPEKEQLHKLAQSQSTMCIYLSAGIVEQVQEELMQAYPPETPVAACYKLTWKEEKIYRGELK
DLAKIVRDNNLTLTTLLVVGEAIDNRKGLSRLYAHEFKHLFRK
>Mature_603_residues
MKAIILISEASLPLAKTLQRELPDTLIYTKNECEGCISITSCHRFIEEHFNDFDSIIFIGAMGICVRSIAGCIKNKYKDP
AVVCVDSTGRFVISVLSGHVGGANELTRHIAAITGGEAVITTQSDNAGLWALDTLAGKYGWKITVPHAEMNRLVTLFVNR
EPTALLLDIKDKGTEYLERTLPAHVKVFYHFEDMPQSEFKLIIAVTPYIYSAEIPMLCFHPAVLHLGIGCRKQCDPSGIA
EYIEAVMHRHGLCPFSLASLNTIELKKDEPLLEILHRRWADTETHIYPAEELKDITVPHPSEKAFEVTGVYGVAESTALK
SSGEGTLVLEKQKGMLTEGNHFTFAIAVSATAMRGGHIEIVGAGPGDPELISVRGKRMLEKADLVLYAGSLVPRELTFYA
KEGATVRSSAGMDLEEQFALMKEFYDKGLFIVRLHTGDPCIYGAIQEQMAFFDRYKMSYHITPGISSFQAAAAALRSQFT
IPEKVQSIILTRGEGRTPMPEKEQLHKLAQSQSTMCIYLSAGIVEQVQEELMQAYPPETPVAACYKLTWKEEKIYRGELK
DLAKIVRDNNLTLTTLLVVGEAIDNRKGLSRLYAHEFKHLFRK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=247, Percent_Identity=25.9109311740891, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322922, Length=326, Percent_Identity=24.8466257668712, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 67018; Mature: 67018

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIILISEASLPLAKTLQRELPDTLIYTKNECEGCISITSCHRFIEEHFNDFDSIIFIG
CCEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
AMGICVRSIAGCIKNKYKDPAVVCVDSTGRFVISVLSGHVGGANELTRHIAAITGGEAVI
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHCCCCCHHHHHHHHHHEECCCEEE
TTQSDNAGLWALDTLAGKYGWKITVPHAEMNRLVTLFVNREPTALLLDIKDKGTEYLERT
EECCCCCCEEEEHHHCCCCCCEEEECHHHHCCEEEEEECCCCCEEEEEECCCCHHHHHHC
LPAHVKVFYHFEDMPQSEFKLIIAVTPYIYSAEIPMLCFHPAVLHLGIGCRKQCDPSGIA
CCCEEEEEEEECCCCCCCEEEEEEEECEEEECCCCCEECCHHHHHHCCCCCCCCCHHHHH
EYIEAVMHRHGLCPFSLASLNTIELKKDEPLLEILHRRWADTETHIYPAEELKDITVPHP
HHHHHHHHHCCCCCEECCCCCEEEECCCCHHHHHHHHHCCCCCCEEECHHHHCCCCCCCC
SEKAFEVTGVYGVAESTALKSSGEGTLVLEKQKGMLTEGNHFTFAIAVSATAMRGGHIEI
CCCCEEEEEEEECCHHHHHCCCCCCEEEEEECCCCEECCCEEEEEEEEEEEEECCCEEEE
VGAGPGDPELISVRGKRMLEKADLVLYAGSLVPRELTFYAKEGATVRSSAGMDLEEQFAL
EECCCCCCCEEEECHHHHHHHCCEEEEECCCCCCEEEEEECCCCEEECCCCCCHHHHHHH
MKEFYDKGLFIVRLHTGDPCIYGAIQEQMAFFDRYKMSYHITPGISSFQAAAAALRSQFT
HHHHHHCCEEEEEEECCCCEEHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHCCC
IPEKVQSIILTRGEGRTPMPEKEQLHKLAQSQSTMCIYLSAGIVEQVQEELMQAYPPETP
CHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCC
VAACYKLTWKEEKIYRGELKDLAKIVRDNNLTLTTLLVVGEAIDNRKGLSRLYAHEFKHL
HHHHEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHH
FRK
HCC
>Mature Secondary Structure
MKAIILISEASLPLAKTLQRELPDTLIYTKNECEGCISITSCHRFIEEHFNDFDSIIFIG
CCEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHH
AMGICVRSIAGCIKNKYKDPAVVCVDSTGRFVISVLSGHVGGANELTRHIAAITGGEAVI
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHCCCCCHHHHHHHHHHEECCCEEE
TTQSDNAGLWALDTLAGKYGWKITVPHAEMNRLVTLFVNREPTALLLDIKDKGTEYLERT
EECCCCCCEEEEHHHCCCCCCEEEECHHHHCCEEEEEECCCCCEEEEEECCCCHHHHHHC
LPAHVKVFYHFEDMPQSEFKLIIAVTPYIYSAEIPMLCFHPAVLHLGIGCRKQCDPSGIA
CCCEEEEEEEECCCCCCCEEEEEEEECEEEECCCCCEECCHHHHHHCCCCCCCCCHHHHH
EYIEAVMHRHGLCPFSLASLNTIELKKDEPLLEILHRRWADTETHIYPAEELKDITVPHP
HHHHHHHHHCCCCCEECCCCCEEEECCCCHHHHHHHHHCCCCCCEEECHHHHCCCCCCCC
SEKAFEVTGVYGVAESTALKSSGEGTLVLEKQKGMLTEGNHFTFAIAVSATAMRGGHIEI
CCCCEEEEEEEECCHHHHHCCCCCCEEEEEECCCCEECCCEEEEEEEEEEEEECCCEEEE
VGAGPGDPELISVRGKRMLEKADLVLYAGSLVPRELTFYAKEGATVRSSAGMDLEEQFAL
EECCCCCCCEEEECHHHHHHHCCEEEEECCCCCCEEEEEECCCCEEECCCCCCHHHHHHH
MKEFYDKGLFIVRLHTGDPCIYGAIQEQMAFFDRYKMSYHITPGISSFQAAAAALRSQFT
HHHHHHCCEEEEEEECCCCEEHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHCCC
IPEKVQSIILTRGEGRTPMPEKEQLHKLAQSQSTMCIYLSAGIVEQVQEELMQAYPPETP
CHHHHHHHEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCC
VAACYKLTWKEEKIYRGELKDLAKIVRDNNLTLTTLLVVGEAIDNRKGLSRLYAHEFKHL
HHHHEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHH
FRK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]