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The map label for this gene is mtbA [H]

Identifier: 150005444

GI number: 150005444

Start: 3716362

End: 3717372

Strand: Direct

Name: mtbA [H]

Synonym: BVU_2927

Alternate gene names: 150005444

Gene position: 3716362-3717372 (Clockwise)

Preceding gene: 150005442

Following gene: 150005445

Centisome position: 71.98

GC content: 47.28

Gene sequence:

>1011_bases
ATGAGTAAACTGAATATGAAAGAGTGGATAAACGAAATTATCCAAAAGAAAGAAGTGGTGGCTATGCCCATCATGACCCA
TCCGGGCATAGAGATGATAGGGAAAACGGTACGCGATGCCGTCACTGACGGACAAGTGCATTACAACGCCATCAAGGCAT
TGAGTGAGAAATATCCCACAGCCGCCGCAACCGTGATTATGGATTTGACGGTAGAAGCCGAGGCTTTCGGAGCGGAGATT
GTCTTCCCCGAAAATGAGGTTCCCAGTGTGGTGGGGCGTCTTCTCAATGATGAAGAAGCTATTGACAAACTGGAAATCCC
TGCCTTGAATAAAGGAAGAATCCCCCAGTATCTGAAAGCAAATATGCTGGCAGCCAAGACCATTACGGACCGGCCCGTCT
TTGCCGGTTGCATCGGCCCTTACTCATTGGCAGGACGTTTGTATGACATGTCTGAAATCATGATGCTTATTTATATCAAT
CCGGAAGCGGCCAATTCGCTGTTGAAGAAATGTTCGGATTTCATTCTCCGTTACTGTATGGCATTGAAGGCCACAGGAGT
GAATGGGGTAGTGATGGCCGAGCCGGCTGCCGGACTCTTGTCTGATGAGGACTGCACGCAATACTCGTCTGTTTTCATTA
AGGAGATAGTAGAGAAAGTGCAGGACGATCACTTCACGGTCATTCTGCATAATTGTGGAAATACCGGTCATTGTACCAAG
GCAATGGTAGCTACAGGAGCTGCCGCTTATCATTTTGGAAATAAAATAGATATGGTCGAAGCTCTGAAAGAAGTACCTGC
CGATGCCCTTGCAATGGGAAATCTTGATCCGGTGAGCTTGTTCAAGGCGGCTACTCCGGAGGTGATGAAGAAAGCCACAC
TCGATTTGTTGGAAGCTACCCGGTCCTATCCCAACTTCGTGCTCTCCAGCGGATGTGATACACCGCCTCATACGCCATCT
GAAAATATTGATGCATTCTTTGCCGCATTAAATGAATTCAATAATGCTTGA

Upstream 100 bases:

>100_bases
AATGAATGTCCCTATTTACATATTTAAATTTATGTATGAGTACAATGTTTTTTCATATGCGTCATTAAACATAGCCAATA
TAGGTAAAGAATAAAAGACC

Downstream 100 bases:

>100_bases
AAAAGAACTTTCTTTTGCTGATCTGTCTATCTCATTGTCCGAACTCTATGAGGCAATGGGGTATGCAGATGCTGTGCCCG
ACGAAGCGGTAGAAAAAGAA

Product: methylcobamide:CoM methyltransferase mtbA

Products: CO2; Coproporphyrinogen III [C]

Alternate protein names: MT2-A; Methylcobamide:CoM methyltransferase II isozyme A [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSKLNMKEWINEIIQKKEVVAMPIMTHPGIEMIGKTVRDAVTDGQVHYNAIKALSEKYPTAAATVIMDLTVEAEAFGAEI
VFPENEVPSVVGRLLNDEEAIDKLEIPALNKGRIPQYLKANMLAAKTITDRPVFAGCIGPYSLAGRLYDMSEIMMLIYIN
PEAANSLLKKCSDFILRYCMALKATGVNGVVMAEPAAGLLSDEDCTQYSSVFIKEIVEKVQDDHFTVILHNCGNTGHCTK
AMVATGAAAYHFGNKIDMVEALKEVPADALAMGNLDPVSLFKAATPEVMKKATLDLLEATRSYPNFVLSSGCDTPPHTPS
ENIDAFFAALNEFNNA

Sequences:

>Translated_336_residues
MSKLNMKEWINEIIQKKEVVAMPIMTHPGIEMIGKTVRDAVTDGQVHYNAIKALSEKYPTAAATVIMDLTVEAEAFGAEI
VFPENEVPSVVGRLLNDEEAIDKLEIPALNKGRIPQYLKANMLAAKTITDRPVFAGCIGPYSLAGRLYDMSEIMMLIYIN
PEAANSLLKKCSDFILRYCMALKATGVNGVVMAEPAAGLLSDEDCTQYSSVFIKEIVEKVQDDHFTVILHNCGNTGHCTK
AMVATGAAAYHFGNKIDMVEALKEVPADALAMGNLDPVSLFKAATPEVMKKATLDLLEATRSYPNFVLSSGCDTPPHTPS
ENIDAFFAALNEFNNA
>Mature_335_residues
SKLNMKEWINEIIQKKEVVAMPIMTHPGIEMIGKTVRDAVTDGQVHYNAIKALSEKYPTAAATVIMDLTVEAEAFGAEIV
FPENEVPSVVGRLLNDEEAIDKLEIPALNKGRIPQYLKANMLAAKTITDRPVFAGCIGPYSLAGRLYDMSEIMMLIYINP
EAANSLLKKCSDFILRYCMALKATGVNGVVMAEPAAGLLSDEDCTQYSSVFIKEIVEKVQDDHFTVILHNCGNTGHCTKA
MVATGAAAYHFGNKIDMVEALKEVPADALAMGNLDPVSLFKAATPEVMKKATLDLLEATRSYPNFVLSSGCDTPPHTPSE
NIDAFFAALNEFNNA

Specific function: Catalyzes the transfer of the methyl group from the methylated corrinoid cofactor of mtmC, mtbC and mttC to coenzyme M [H]

COG id: COG0407

COG function: function code H; Uroporphyrinogen-III decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen decarboxylase family. MtbA/mtaA subfamily [H]

Homologues:

Organism=Escherichia coli, GI2367337, Length=294, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006360
- InterPro:   IPR000257 [H]

Pfam domain/function: PF01208 URO-D [H]

EC number: 4.1.1.37 [C]

Molecular weight: Translated: 36571; Mature: 36439

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLNMKEWINEIIQKKEVVAMPIMTHPGIEMIGKTVRDAVTDGQVHYNAIKALSEKYPT
CCCCCHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
AAATVIMDLTVEAEAFGAEIVFPENEVPSVVGRLLNDEEAIDKLEIPALNKGRIPQYLKA
HHHHHHHHEEEEHHHCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHH
NMLAAKTITDRPVFAGCIGPYSLAGRLYDMSEIMMLIYINPEAANSLLKKCSDFILRYCM
HHHHHHHCCCCCCHHHCCCHHHHHHHHCCHHHEEEEEEECHHHHHHHHHHHHHHHHHHHH
ALKATGVNGVVMAEPAAGLLSDEDCTQYSSVFIKEIVEKVQDDHFTVILHNCGNTGHCTK
HHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
AMVATGAAAYHFGNKIDMVEALKEVPADALAMGNLDPVSLFKAATPEVMKKATLDLLEAT
HHHHHCHHHHHCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
RSYPNFVLSSGCDTPPHTPSENIDAFFAALNEFNNA
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLNMKEWINEIIQKKEVVAMPIMTHPGIEMIGKTVRDAVTDGQVHYNAIKALSEKYPT
CCCCHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCH
AAATVIMDLTVEAEAFGAEIVFPENEVPSVVGRLLNDEEAIDKLEIPALNKGRIPQYLKA
HHHHHHHHEEEEHHHCCCEEECCCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHH
NMLAAKTITDRPVFAGCIGPYSLAGRLYDMSEIMMLIYINPEAANSLLKKCSDFILRYCM
HHHHHHHCCCCCCHHHCCCHHHHHHHHCCHHHEEEEEEECHHHHHHHHHHHHHHHHHHHH
ALKATGVNGVVMAEPAAGLLSDEDCTQYSSVFIKEIVEKVQDDHFTVILHNCGNTGHCTK
HHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
AMVATGAAAYHFGNKIDMVEALKEVPADALAMGNLDPVSLFKAATPEVMKKATLDLLEAT
HHHHHCHHHHHCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
RSYPNFVLSSGCDTPPHTPSENIDAFFAALNEFNNA
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Reducing agents [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00231 {7-COOH,} 0.00053 {8-COOH-porphyrinogen} 0.00025 {8-COOH-porphyrinogen} 0.00533 {uroporphyrinogen} 0.00256 {uroporphyrinogen} 0.00133 {uroporphyrinogen} 0.001 {uroporphyrinogen} 0.0008 {uroporphyrinogen} 0.0004 {uroporphyrino

Substrates: H+; Uroporphyrinogen III [C]

Specific reaction: (4) H+ + Uroporphyrinogen III --> (4) CO2 + Coproporphyrinogen III [C]

General reaction: Decarboxylation [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); 7-COOH-porphyrinogenI; 7-COOH-porphyrinogen III; Butanedione; Diethyl dicarbonate; Fe2+; Iodoacetamide; p-Hydroxy mercuribenzoate; Phenyl glyoxal; Pt2+; Sn2+; UroporphyrinogenI uroporphyrinogen III; Uroporphyrinogen III;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]