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The map label for this gene is purT

Identifier: 150005296

GI number: 150005296

Start: 3532344

End: 3533513

Strand: Direct

Name: purT

Synonym: BVU_2768

Alternate gene names: 150005296

Gene position: 3532344-3533513 (Clockwise)

Preceding gene: 150005295

Following gene: 150005302

Centisome position: 68.41

GC content: 46.24

Gene sequence:

>1170_bases
ATGACTAAGAAGATTTTATTGCTGGGTTCTGGCGAATTAGGTAAGGAATTCGTCATTGCGGCAAAACGTAAAGGGCAGTA
TGTTATTGCTTGCGACTCTTATGCAGGTGCTCCGGCCATGCAGGTGGCTGATGAATTTGAAGTGTTCAGTATGCTTGACG
GTGATGCGTTGGATGCTGTGGTAGCCAAACACAAGCCGGATATAATTGTGCCTGAAATTGAAGCTATCCGTACCGAACGT
CTGTATCATTTGGAACAGGAAGGTATTCAAGTAGTGCCGAGTGCCCGTGCAGTGAATTTCACGATGAACCGCAAGGCCAT
TCGTGATTTGGCAGCTAAGGAATTAGGATTAAAGACAGCCAAGTATTTCTATGCCAAGTCTTTGGAAGAATTGCAGGAGG
CCGCAAAAGAGATTGGTTTTCCCTGTGTGGTGAAACCTTTGATGTCTTCATCCGGCAAAGGCCAGTCTTTGGTCAAGAGC
GCGGATGAGTTGGAACAGGCATGGCATTATGGGTGTGAAGGCAGTCGTGGAGATATTAAAGAATTGATCATTGAGGAATT
TATTCAGTTCGATAGTGAGATTACTTTGCTCACCGTCACTCAGAAAAACGGTCCTACTTTGTTCTGTCCGCCTATCGGAC
ATGTACAGAAAGGGGGAGATTATAGGGAAAGTTTCCAGCCGGCGCACATTGATCCCGAACATTTGAAGGAGGCACAGCGT
ATGGCCGATAAAGTGACGGCGGCTTTGACCGGTGCGGGAATCTGGGGAGTTGAATTTTTCTTGAGTCATGAAAACGGAGT
TTATTTCTCCGAACTTTCTCCCCGTCCGCATGATACCGGAATGGTGACTTTGGCAGGTACTCAGAATTTAAATGAGTTTG
AGTTGCATCTGCGTGCGGTGTTAGGCCTTCCTATCCCTGAAATTACACAAGAACGCATAGGGGCCAGTGCAGTGATTCTT
TCTCCAATTGCCAGCAAGGAAGCTCCCCGTTATCGTGGTGAAGAGGAAGTTTGTAAGGAGACAAATACTTATCTCCGTAT
TTTTGGTAAACCTTATACAAAATTGAACCGCCGTATGGGAGTTGTGGTTTGTTATGCTCCTAATGGCAGTGACTTGGACG
CTTTGCGTGACAAGTGCAAGGCTGCTGCTGCAAAGGTAGAGGTGTATTAA

Upstream 100 bases:

>100_bases
TAAAATACACTATCTTTGCAGATGGAAAGTTGGGAATCAAGAGGTGTTTTGTTCTCAACTCTCCGTTTTTTGTAAGGAAT
ATCAAACATATATAAATACT

Downstream 100 bases:

>100_bases
TATCTTGTATTGATGGATATAAAATAAAGGTCCGGGCTTTCCCAAGTCCGGACTTTTTGTTGTTCCGAAGGTATGCTTCC
CTGGAACAAGGTTTTATAGT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTKKILLLGSGELGKEFVIAAKRKGQYVIACDSYAGAPAMQVADEFEVFSMLDGDALDAVVAKHKPDIIVPEIEAIRTER
LYHLEQEGIQVVPSARAVNFTMNRKAIRDLAAKELGLKTAKYFYAKSLEELQEAAKEIGFPCVVKPLMSSSGKGQSLVKS
ADELEQAWHYGCEGSRGDIKELIIEEFIQFDSEITLLTVTQKNGPTLFCPPIGHVQKGGDYRESFQPAHIDPEHLKEAQR
MADKVTAALTGAGIWGVEFFLSHENGVYFSELSPRPHDTGMVTLAGTQNLNEFELHLRAVLGLPIPEITQERIGASAVIL
SPIASKEAPRYRGEEEVCKETNTYLRIFGKPYTKLNRRMGVVVCYAPNGSDLDALRDKCKAAAAKVEVY

Sequences:

>Translated_389_residues
MTKKILLLGSGELGKEFVIAAKRKGQYVIACDSYAGAPAMQVADEFEVFSMLDGDALDAVVAKHKPDIIVPEIEAIRTER
LYHLEQEGIQVVPSARAVNFTMNRKAIRDLAAKELGLKTAKYFYAKSLEELQEAAKEIGFPCVVKPLMSSSGKGQSLVKS
ADELEQAWHYGCEGSRGDIKELIIEEFIQFDSEITLLTVTQKNGPTLFCPPIGHVQKGGDYRESFQPAHIDPEHLKEAQR
MADKVTAALTGAGIWGVEFFLSHENGVYFSELSPRPHDTGMVTLAGTQNLNEFELHLRAVLGLPIPEITQERIGASAVIL
SPIASKEAPRYRGEEEVCKETNTYLRIFGKPYTKLNRRMGVVVCYAPNGSDLDALRDKCKAAAAKVEVY
>Mature_388_residues
TKKILLLGSGELGKEFVIAAKRKGQYVIACDSYAGAPAMQVADEFEVFSMLDGDALDAVVAKHKPDIIVPEIEAIRTERL
YHLEQEGIQVVPSARAVNFTMNRKAIRDLAAKELGLKTAKYFYAKSLEELQEAAKEIGFPCVVKPLMSSSGKGQSLVKSA
DELEQAWHYGCEGSRGDIKELIIEEFIQFDSEITLLTVTQKNGPTLFCPPIGHVQKGGDYRESFQPAHIDPEHLKEAQRM
ADKVTAALTGAGIWGVEFFLSHENGVYFSELSPRPHDTGMVTLAGTQNLNEFELHLRAVLGLPIPEITQERIGASAVILS
PIASKEAPRYRGEEEVCKETNTYLRIFGKPYTKLNRRMGVVVCYAPNGSDLDALRDKCKAAAAKVEVY

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=385, Percent_Identity=50.9090909090909, Blast_Score=354, Evalue=4e-99,
Organism=Escherichia coli, GI1786216, Length=302, Percent_Identity=25.4966887417219, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786733, Length=326, Percent_Identity=25.1533742331288, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324702, Length=370, Percent_Identity=23.2432432432432, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_BACV8 (A6L404)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300040.1
- ProteinModelPortal:   A6L404
- SMR:   A6L404
- STRING:   A6L404
- GeneID:   5303731
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2768
- NMPDR:   fig|435590.6.peg.2603
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- ProtClustDB:   PRK09288
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42894; Mature: 42762

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 72-72 BINDING 104-104 BINDING 145-145 BINDING 193-193 BINDING 258-258 BINDING 271-271 BINDING 278-278 BINDING 350-350

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKILLLGSGELGKEFVIAAKRKGQYVIACDSYAGAPAMQVADEFEVFSMLDGDALDAV
CCCEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHH
VAKHKPDIIVPEIEAIRTERLYHLEQEGIQVVPSARAVNFTMNRKAIRDLAAKELGLKTA
HHHCCCCEECCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEECHHHHHHHHHHHHCCHHH
KYFYAKSLEELQEAAKEIGFPCVVKPLMSSSGKGQSLVKSADELEQAWHYGCEGSRGDIK
HHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
ELIIEEFIQFDSEITLLTVTQKNGPTLFCPPIGHVQKGGDYRESFQPAHIDPEHLKEAQR
HHHHHHHHHCCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHH
MADKVTAALTGAGIWGVEFFLSHENGVYFSELSPRPHDTGMVTLAGTQNLNEFELHLRAV
HHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHH
LGLPIPEITQERIGASAVILSPIASKEAPRYRGEEEVCKETNTYLRIFGKPYTKLNRRMG
HCCCCCHHHHHHCCCCEEEECCHHCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHCCCCC
VVVCYAPNGSDLDALRDKCKAAAAKVEVY
EEEEECCCCCCHHHHHHHHHHHHHCEECC
>Mature Secondary Structure 
TKKILLLGSGELGKEFVIAAKRKGQYVIACDSYAGAPAMQVADEFEVFSMLDGDALDAV
CCEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHH
VAKHKPDIIVPEIEAIRTERLYHLEQEGIQVVPSARAVNFTMNRKAIRDLAAKELGLKTA
HHHCCCCEECCCHHHHHHHHHHHHHHCCEEEECCCEEEEEEECHHHHHHHHHHHHCCHHH
KYFYAKSLEELQEAAKEIGFPCVVKPLMSSSGKGQSLVKSADELEQAWHYGCEGSRGDIK
HHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
ELIIEEFIQFDSEITLLTVTQKNGPTLFCPPIGHVQKGGDYRESFQPAHIDPEHLKEAQR
HHHHHHHHHCCCCEEEEEEEECCCCEEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHH
MADKVTAALTGAGIWGVEFFLSHENGVYFSELSPRPHDTGMVTLAGTQNLNEFELHLRAV
HHHHHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHH
LGLPIPEITQERIGASAVILSPIASKEAPRYRGEEEVCKETNTYLRIFGKPYTKLNRRMG
HCCCCCHHHHHHCCCCEEEECCHHCCCCCCCCCHHHHHHCCCCEEEEECCCHHHHCCCCC
VVVCYAPNGSDLDALRDKCKAAAAKVEVY
EEEEECCCCCCHHHHHHHHHHHHHCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA