Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 150004794

GI number: 150004794

Start: 2918358

End: 2919245

Strand: Reverse

Name: metH [H]

Synonym: BVU_2257

Alternate gene names: 150004794

Gene position: 2919245-2918358 (Counterclockwise)

Preceding gene: 150004795

Following gene: 150004793

Centisome position: 56.54

GC content: 45.61

Gene sequence:

>888_bases
ATGATTGTAAATTACAAGATTCACGAAGTAAGCGAATACATTAACTGGATTTATTTTTTCCATGCATGGGGCTTTCAACC
CAAGTTTGCCGCCATTGCGGACATACATGGATGTGATTCATGCCGGGCCATGTGGCTCACCGGTTTTGCGGAAGAAGACC
GTCCGAAAGCCAGTGAAGCCATGCAGCTTTTCAAAGAGGCAAATCGGATGTTGAATGAACTGGATACAGCGTATCAGACC
CACGGAGTGGTCAATATCATGGATGCCAATGCCGACGGAGATGATCTGCTGCTGAACAGCAAGCGTTTTCCGCTTTTGCG
TCAACAAACAGCAAAATTGAAAGAAGATGACCCGTTTTTATGTCTGAGTGACTTTGTGCGTCCATTATCATCGGGTATCA
CAGACAAAATAGGAGCATTCGCCACTACGGTAGATGCAGAAATGGAGCGGCTCTATGCAGAAGACGATTACAAACGTATG
TTAGTGCAAACATTGTCCGACAGACTGGCGGAAGCTACTGCCGAAAAACTACACGAAGATGTACGCAAAAAACTTTGGGG
ATATGCACCTGATGAAAACCTAAGCGTAAAAGACCTGCATAATGAAAAATATCAAGGGATACGTCCTGCCGTAGGTTATC
CTTCCTTGCCCGACCAATCCGTCAATTTCCTATTAGACGAGTTGCTGGATATGAAGCAAATAGGAATTTCATTGACCGAG
AATGGTATGATGAAGCCCCATGCCTCTGTATGCGGACTCATGTTTGCCCATCCGGCTTCCCGCTATTTCTCCATCGGAAA
AATAGGTGAAGACCAATTGGCAGATTACGCCGCCCGCCGGGGCATGAGTATAGAAGAAACTAAAAAGTTTCTCGCCGCCA
ATCTATAA

Upstream 100 bases:

>100_bases
TCCACACACGGGTAGAAAAAACATTCGTCAATGGTGATCTGGTTTACAGTGACAACAAGGTAGACCAAAGTCATCGCGGA
CAAGAATTAAGGTTTAAAAG

Downstream 100 bases:

>100_bases
AAAACGAATAAATTATGATGCTGATAAAGTTATTTCTGTTTTTTCTACTGCTACTGATATTGCCCGATATGTATATATAC
AAGGCATATATACGCCGTGT

Product: 5-methyltetrahydrofolate-homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MIVNYKIHEVSEYINWIYFFHAWGFQPKFAAIADIHGCDSCRAMWLTGFAEEDRPKASEAMQLFKEANRMLNELDTAYQT
HGVVNIMDANADGDDLLLNSKRFPLLRQQTAKLKEDDPFLCLSDFVRPLSSGITDKIGAFATTVDAEMERLYAEDDYKRM
LVQTLSDRLAEATAEKLHEDVRKKLWGYAPDENLSVKDLHNEKYQGIRPAVGYPSLPDQSVNFLLDELLDMKQIGISLTE
NGMMKPHASVCGLMFAHPASRYFSIGKIGEDQLADYAARRGMSIEETKKFLAANL

Sequences:

>Translated_295_residues
MIVNYKIHEVSEYINWIYFFHAWGFQPKFAAIADIHGCDSCRAMWLTGFAEEDRPKASEAMQLFKEANRMLNELDTAYQT
HGVVNIMDANADGDDLLLNSKRFPLLRQQTAKLKEDDPFLCLSDFVRPLSSGITDKIGAFATTVDAEMERLYAEDDYKRM
LVQTLSDRLAEATAEKLHEDVRKKLWGYAPDENLSVKDLHNEKYQGIRPAVGYPSLPDQSVNFLLDELLDMKQIGISLTE
NGMMKPHASVCGLMFAHPASRYFSIGKIGEDQLADYAARRGMSIEETKKFLAANL
>Mature_295_residues
MIVNYKIHEVSEYINWIYFFHAWGFQPKFAAIADIHGCDSCRAMWLTGFAEEDRPKASEAMQLFKEANRMLNELDTAYQT
HGVVNIMDANADGDDLLLNSKRFPLLRQQTAKLKEDDPFLCLSDFVRPLSSGITDKIGAFATTVDAEMERLYAEDDYKRM
LVQTLSDRLAEATAEKLHEDVRKKLWGYAPDENLSVKDLHNEKYQGIRPAVGYPSLPDQSVNFLLDELLDMKQIGISLTE
NGMMKPHASVCGLMFAHPASRYFSIGKIGEDQLADYAARRGMSIEETKKFLAANL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=313, Percent_Identity=37.3801916932907, Blast_Score=191, Evalue=8e-49,
Organism=Escherichia coli, GI1790450, Length=311, Percent_Identity=41.4790996784566, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17535405, Length=307, Percent_Identity=38.4364820846906, Blast_Score=194, Evalue=3e-50,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 33462; Mature: 33462

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50974 ADOMET_ACTIVATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVNYKIHEVSEYINWIYFFHAWGFQPKFAAIADIHGCDSCRAMWLTGFAEEDRPKASEA
CEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHH
MQLFKEANRMLNELDTAYQTHGVVNIMDANADGDDLLLNSKRFPLLRQQTAKLKEDDPFL
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCHHHHHHHCCCCCCCCC
CLSDFVRPLSSGITDKIGAFATTVDAEMERLYAEDDYKRMLVQTLSDRLAEATAEKLHED
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRKKLWGYAPDENLSVKDLHNEKYQGIRPAVGYPSLPDQSVNFLLDELLDMKQIGISLTE
HHHHHCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEECC
NGMMKPHASVCGLMFAHPASRYFSIGKIGEDQLADYAARRGMSIEETKKFLAANL
CCCCCCHHHHHHHHHHCCHHHHEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIVNYKIHEVSEYINWIYFFHAWGFQPKFAAIADIHGCDSCRAMWLTGFAEEDRPKASEA
CEEECHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHH
MQLFKEANRMLNELDTAYQTHGVVNIMDANADGDDLLLNSKRFPLLRQQTAKLKEDDPFL
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCHHHHHHHCCCCCCCCC
CLSDFVRPLSSGITDKIGAFATTVDAEMERLYAEDDYKRMLVQTLSDRLAEATAEKLHED
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VRKKLWGYAPDENLSVKDLHNEKYQGIRPAVGYPSLPDQSVNFLLDELLDMKQIGISLTE
HHHHHCCCCCCCCCCHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEECC
NGMMKPHASVCGLMFAHPASRYFSIGKIGEDQLADYAARRGMSIEETKKFLAANL
CCCCCCHHHHHHHHHHCCHHHHEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185137; 2668277; 8369296; 8265357; 9278503; 1593636; 7992050; 8939751 [H]