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The map label for this gene is pgl [H]

Identifier: 150004702

GI number: 150004702

Start: 2786055

End: 2787470

Strand: Direct

Name: pgl [H]

Synonym: BVU_2163

Alternate gene names: 150004702

Gene position: 2786055-2787470 (Clockwise)

Preceding gene: 150004701

Following gene: 150004704

Centisome position: 53.96

GC content: 44.77

Gene sequence:

>1416_bases
ATGACAACATTAAAAGATTTATTTGCTGGTTTGCTTCTTGTTATCTTCTTTCCCATTAGCTCATGGGCTGAAGGGGTGAA
GCTTCAGCCGGTGGAGGTAGAGGCTCCTTTCCCTATGGACTCTGTATTTTTGTGTATATTCCCACAGAGGGATTTCTTGA
TAACCAAATATGGAGCTAAGGCAGGAGGAAAAAAACTGAATACAAAGGCAATAGCCAAGGCAATCACTGCCTGTCATTTG
GTAGGTGGCGGTCGTGTAGTCATTCCCAAAGGAGAATGGCTGACTGGTCCTATCCACTTGAAAAGCAATATAAACCTCTA
CATGGAGGAAGGAGCGGTATTGCGTTTTACAGACACTCCTTCGGATTATCTTCCGGCGGTAATGACCTCATGGGAAGGCA
TGGAGTGTTATAACTACTCTCCGCTAATTTATGCATCTGATTGCGAAAACATCGCCATTACAGGAAAAGGAGTATTGGCT
CCCAAGATGGATACATGGAGAATATGGTTTGCCCGTCCGCAAGCTCACATGGAGGCTTTGAGGAAGTTATATACAATGGC
TTCCACTGATGTTCCTGTGGAAGAAAGGCAAATGGCTGTTGGTGAGAATAACCTCCGTCCTCATCTGATTCATCTGAACC
GTTGCAGGAATGTGTTGCTTGACGGCTTTAAGATCAGGGAAAGTCCCTTTTGGACTATACACCTTTATATGTGCAAGGGT
GGCATTGTCCGTGGTCTTGATGTCAAGGCGAACGGCCACAACAACGATGGCATAGATTTGGAAATGAGCCGTAATTTCCT
GATAGAGAATTGCAAGTTTGACCAGGGAGATGATGCTGTAGTTATTAAATCGGGGCGAAACCGAGATGCGTGGAGATTGG
GCACCCCTTGTGAGAATATCGTTGTACGTAATTGTCAGGTAATGGAAGGACATACTCTATTGGGCATTGGCAGTGAACTG
TCTGGAGGTGTGCGAAACGTTTATATGCATCATTGTGATGTACCCGCATCTGTGCATTGCTTATTCTTTATCAAAACCAA
CCGAAGGCGGGGTGGAATTGTGGAAAACATCTACTTGGAAGATGTTACGTGCAAGGATACTGAATATTTGGTTGGGTTGG
ACATGGATATTCTCTATCAATGGCGGGAGTTGGTTCCTACTTATGAGGAACGTCTTACCCGTATCGAGGGAATTCATGTG
AAGGACATTCGGTGTGGTTCCGCGGATTTTGTCTATGAGTTGTGCGGTGACGAAAGGTTGCCACCCAAGGACATTACTAT
TCGAAACATTGTAGTGGATAATTCCAAGGGAGTCCCCAATCAGAGCCATCATATTACAGGACTTACATTGGATAGTATTT
GTTATTCTCATATTCGGCCGGATATGGTGACAAAACCACGGAAAAGATGGCGCTGA

Upstream 100 bases:

>100_bases
GACGAAGTAAGGGGATGATGAGCGTAAAGGTTTGCGTAGAAGGCCTTCCTGAACAAACAATAACGATAAAAGTACACTAA
TTTTTATAATAGCAGTAAGA

Downstream 100 bases:

>100_bases
AGGCTGTGCTATTGTGTTGTCAAGAATGAATAATATTCACAAGGGCTAAGAAGTTAGAAGAAGTTGTCGCCCGCTCCGGG
CGAGCTTAGAAGTTAAAGTC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: PGL; Pectinase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTTLKDLFAGLLLVIFFPISSWAEGVKLQPVEVEAPFPMDSVFLCIFPQRDFLITKYGAKAGGKKLNTKAIAKAITACHL
VGGGRVVIPKGEWLTGPIHLKSNINLYMEEGAVLRFTDTPSDYLPAVMTSWEGMECYNYSPLIYASDCENIAITGKGVLA
PKMDTWRIWFARPQAHMEALRKLYTMASTDVPVEERQMAVGENNLRPHLIHLNRCRNVLLDGFKIRESPFWTIHLYMCKG
GIVRGLDVKANGHNNDGIDLEMSRNFLIENCKFDQGDDAVVIKSGRNRDAWRLGTPCENIVVRNCQVMEGHTLLGIGSEL
SGGVRNVYMHHCDVPASVHCLFFIKTNRRRGGIVENIYLEDVTCKDTEYLVGLDMDILYQWRELVPTYEERLTRIEGIHV
KDIRCGSADFVYELCGDERLPPKDITIRNIVVDNSKGVPNQSHHITGLTLDSICYSHIRPDMVTKPRKRWR

Sequences:

>Translated_471_residues
MTTLKDLFAGLLLVIFFPISSWAEGVKLQPVEVEAPFPMDSVFLCIFPQRDFLITKYGAKAGGKKLNTKAIAKAITACHL
VGGGRVVIPKGEWLTGPIHLKSNINLYMEEGAVLRFTDTPSDYLPAVMTSWEGMECYNYSPLIYASDCENIAITGKGVLA
PKMDTWRIWFARPQAHMEALRKLYTMASTDVPVEERQMAVGENNLRPHLIHLNRCRNVLLDGFKIRESPFWTIHLYMCKG
GIVRGLDVKANGHNNDGIDLEMSRNFLIENCKFDQGDDAVVIKSGRNRDAWRLGTPCENIVVRNCQVMEGHTLLGIGSEL
SGGVRNVYMHHCDVPASVHCLFFIKTNRRRGGIVENIYLEDVTCKDTEYLVGLDMDILYQWRELVPTYEERLTRIEGIHV
KDIRCGSADFVYELCGDERLPPKDITIRNIVVDNSKGVPNQSHHITGLTLDSICYSHIRPDMVTKPRKRWR
>Mature_470_residues
TTLKDLFAGLLLVIFFPISSWAEGVKLQPVEVEAPFPMDSVFLCIFPQRDFLITKYGAKAGGKKLNTKAIAKAITACHLV
GGGRVVIPKGEWLTGPIHLKSNINLYMEEGAVLRFTDTPSDYLPAVMTSWEGMECYNYSPLIYASDCENIAITGKGVLAP
KMDTWRIWFARPQAHMEALRKLYTMASTDVPVEERQMAVGENNLRPHLIHLNRCRNVLLDGFKIRESPFWTIHLYMCKGG
IVRGLDVKANGHNNDGIDLEMSRNFLIENCKFDQGDDAVVIKSGRNRDAWRLGTPCENIVVRNCQVMEGHTLLGIGSELS
GGVRNVYMHHCDVPASVHCLFFIKTNRRRGGIVENIYLEDVTCKDTEYLVGLDMDILYQWRELVPTYEERLTRIEGIHVK
DIRCGSADFVYELCGDERLPPKDITIRNIVVDNSKGVPNQSHHITGLTLDSICYSHIRPDMVTKPRKRWR

Specific function: Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PbH1 repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR006626
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 53317; Mature: 53186

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLKDLFAGLLLVIFFPISSWAEGVKLQPVEVEAPFPMDSVFLCIFPQRDFLITKYGAK
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCC
AGGKKLNTKAIAKAITACHLVGGGRVVIPKGEWLTGPIHLKSNINLYMEEGAVLRFTDTP
CCCCCCCHHHHHHHHHHHEEECCCEEEEECCCEEECCEEEECCCEEEEECCCEEEEECCC
SDYLPAVMTSWEGMECYNYSPLIYASDCENIAITGKGVLAPKMDTWRIWFARPQAHMEAL
CHHHHHHHCCCCCCEEECCCCEEEECCCCEEEEECCEEECCCCCEEEEEEECCHHHHHHH
RKLYTMASTDVPVEERQMAVGENNLRPHLIHLNRCRNVLLDGFKIRESPFWTIHLYMCKG
HHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCEEEECCCEEEEEEEEECC
GIVRGLDVKANGHNNDGIDLEMSRNFLIENCKFDQGDDAVVIKSGRNRDAWRLGTPCENI
CEEEEEEEEECCCCCCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCCEECCCCHHHH
VVRNCQVMEGHTLLGIGSELSGGVRNVYMHHCDVPASVHCLFFIKTNRRRGGIVENIYLE
EEECEEEECCCEEEEECCCCCCCHHEEEEEECCCCCCEEEEEEEEECCCCCCEEEEEEEE
DVTCKDTEYLVGLDMDILYQWRELVPTYEERLTRIEGIHVKDIRCGSADFVYELCGDERL
CEECCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCC
PPKDITIRNIVVDNSKGVPNQSHHITGLTLDSICYSHIRPDMVTKPRKRWR
CCCCEEEEEEEEECCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCHHHCC
>Mature Secondary Structure 
TTLKDLFAGLLLVIFFPISSWAEGVKLQPVEVEAPFPMDSVFLCIFPQRDFLITKYGAK
CCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCEEEEECCCC
AGGKKLNTKAIAKAITACHLVGGGRVVIPKGEWLTGPIHLKSNINLYMEEGAVLRFTDTP
CCCCCCCHHHHHHHHHHHEEECCCEEEEECCCEEECCEEEECCCEEEEECCCEEEEECCC
SDYLPAVMTSWEGMECYNYSPLIYASDCENIAITGKGVLAPKMDTWRIWFARPQAHMEAL
CHHHHHHHCCCCCCEEECCCCEEEECCCCEEEEECCEEECCCCCEEEEEEECCHHHHHHH
RKLYTMASTDVPVEERQMAVGENNLRPHLIHLNRCRNVLLDGFKIRESPFWTIHLYMCKG
HHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCEEEECCCEEEEEEEEECC
GIVRGLDVKANGHNNDGIDLEMSRNFLIENCKFDQGDDAVVIKSGRNRDAWRLGTPCENI
CEEEEEEEEECCCCCCCEEEEECCCEEEECCCCCCCCCEEEEECCCCCCCEECCCCHHHH
VVRNCQVMEGHTLLGIGSELSGGVRNVYMHHCDVPASVHCLFFIKTNRRRGGIVENIYLE
EEECEEEECCCEEEEECCCCCCCHHEEEEEECCCCCCEEEEEEEEECCCCCCEEEEEEEE
DVTCKDTEYLVGLDMDILYQWRELVPTYEERLTRIEGIHVKDIRCGSADFVYELCGDERL
CEECCCCCEEEECCHHHHHHHHHHCCHHHHHHHHHCCEEEEEEECCCHHHHHHHHCCCCC
PPKDITIRNIVVDNSKGVPNQSHHITGLTLDSICYSHIRPDMVTKPRKRWR
CCCCEEEEEEEEECCCCCCCCCCEEEEEEHHHHHHHHCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1860822 [H]