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The map label for this gene is leuB [H]

Identifier: 150004553

GI number: 150004553

Start: 2598528

End: 2599589

Strand: Direct

Name: leuB [H]

Synonym: BVU_2008

Alternate gene names: 150004553

Gene position: 2598528-2599589 (Clockwise)

Preceding gene: 150004552

Following gene: 150004554

Centisome position: 50.33

GC content: 44.07

Gene sequence:

>1062_bases
ATGGATTTTAAAATTGCAGTTTTAGCCGGTGACGGTATCGGACCGGAGATTTCAGTGCAAGGCGTTGATGTGATGAACGC
TGTTTGTGAGAAGTTTGGCCATAAAGTAAGTTATGAATACGCGATCTGTGGTGCAGATGCGATAGATAAAGTAGGTGATC
CGTTTCCCGAAGCTACTTTTCAGGCTTGTAAGAATGCCGATGCAGTGCTTTTCTCGGCTGTAGGCGACCCTAAATTTGAT
AATGATCCTACAGCAAAGGTTCGTCCGGAGCAAGGTTTGCTGGCTATGCGTAAAAAATTAGGTTTGTTTGCCAATATCCG
TCCTGTGCAGACTTTCAAATGCTTGGTTCATAAGTCTCCGTTGCGCGCCGAATTGGTGGAAAATGCCGATTTTATCTGTA
TTCGTGAACTGACCGGAGGGATGTACTTCGGTGAAAAATATCAGGATAATGATAAGGCATATGATACCAATATGTATACC
CGTCCGGAGATTGAGCGTATTTTAAAAGTGGCTTTTGAATATGCCATGAAGCGCCGTAAGCATCTTACTGTAGTTGATAA
GGCAAATGTGCTGGCATCTTCACGTCTGTGGAGGCAGATTGCACAAGAGATGGCTCCTCAGTATCCGGAAGTGACTACAG
ACTATATGTTTGTGGATAATGCTGCCATGAAGATGCTTCAGGATCCTTGTTTCTTTGACGTGATGGTGACTGAGAATACA
TTTGGTGATATTCTGACAGATGAAGGCTCTGTTATCAGTGGCTCTATGGGATTACTTCCTTCTGCTTCTACGGGTGAAAG
CACTCCGGTATTCGAACCTATTCACGGTTCATGGCCACAGGCTAAAGGATTGAATATTGCCAATCCATTGGCTCAAATCC
TATCGGTGGCCATGCTGTTTGAGTATTTTGATCTTAAGGAGGAAGGCACCTTGATTCGTAAGGCTGTAGATGCTTCTTTG
GATGAAAATGTGCGTACTCCGGAAATTCAGGTTGAAGGTGGGGCCAAGTATGGTACTAAGGAAGTGGGTTCCTGGATTGT
GGATTATATCAAGAAAGCATAG

Upstream 100 bases:

>100_bases
TGGTTAGTGATTAGTGGTTAGTGGTTAATGAACAGTGTCGTCAGATTTCATTAACCGCCAACTATTAATCACTGACCGCT
AACCATTAATCACTAAAAAA

Downstream 100 bases:

>100_bases
AATTTTATAGTTTTTTTACGGAGAATCAGAAGTGAATGCCATTTTACTTCTGATTCTTTATTTTTATTGGATAAGAAAAA
GTATATTTGATGCTGCAAAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MDFKIAVLAGDGIGPEISVQGVDVMNAVCEKFGHKVSYEYAICGADAIDKVGDPFPEATFQACKNADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLVHKSPLRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNMYT
RPEIERILKVAFEYAMKRRKHLTVVDKANVLASSRLWRQIAQEMAPQYPEVTTDYMFVDNAAMKMLQDPCFFDVMVTENT
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDLKEEGTLIRKAVDASL
DENVRTPEIQVEGGAKYGTKEVGSWIVDYIKKA

Sequences:

>Translated_353_residues
MDFKIAVLAGDGIGPEISVQGVDVMNAVCEKFGHKVSYEYAICGADAIDKVGDPFPEATFQACKNADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLVHKSPLRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNMYT
RPEIERILKVAFEYAMKRRKHLTVVDKANVLASSRLWRQIAQEMAPQYPEVTTDYMFVDNAAMKMLQDPCFFDVMVTENT
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDLKEEGTLIRKAVDASL
DENVRTPEIQVEGGAKYGTKEVGSWIVDYIKKA
>Mature_353_residues
MDFKIAVLAGDGIGPEISVQGVDVMNAVCEKFGHKVSYEYAICGADAIDKVGDPFPEATFQACKNADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLVHKSPLRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNMYT
RPEIERILKVAFEYAMKRRKHLTVVDKANVLASSRLWRQIAQEMAPQYPEVTTDYMFVDNAAMKMLQDPCFFDVMVTENT
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDLKEEGTLIRKAVDASL
DENVRTPEIQVEGGAKYGTKEVGSWIVDYIKKA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=322, Percent_Identity=30.1242236024845, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI28178816, Length=321, Percent_Identity=28.3489096573209, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178821, Length=320, Percent_Identity=28.4375, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4758582, Length=345, Percent_Identity=28.4057971014493, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=28.8753799392097, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI28178819, Length=159, Percent_Identity=35.2201257861635, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=45.7063711911357, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1788101, Length=345, Percent_Identity=32.7536231884058, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787381, Length=397, Percent_Identity=22.1662468513854, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=349, Percent_Identity=29.512893982808, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17550882, Length=342, Percent_Identity=29.2397660818713, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=27.0949720670391, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17505779, Length=334, Percent_Identity=28.4431137724551, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=40.5479452054795, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=31.7548746518106, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=358, Percent_Identity=30.4469273743017, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=331, Percent_Identity=29.9093655589124, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24643268, Length=323, Percent_Identity=30.6501547987616, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643270, Length=323, Percent_Identity=30.6501547987616, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24661184, Length=336, Percent_Identity=27.6785714285714, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281362242, Length=329, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648872, Length=329, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078639, Length=254, Percent_Identity=28.3464566929134, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078637, Length=254, Percent_Identity=28.3464566929134, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078633, Length=254, Percent_Identity=28.3464566929134, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24650122, Length=254, Percent_Identity=28.3464566929134, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161078635, Length=254, Percent_Identity=28.3464566929134, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20130355, Length=209, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39088; Mature: 39088

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKIAVLAGDGIGPEISVQGVDVMNAVCEKFGHKVSYEYAICGADAIDKVGDPFPEATF
CCEEEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCHHHH
QACKNADAVLFSAVGDPKFDNDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLVHKSP
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHCCC
LRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNMYTRPEIERILKVAFEYAMKRRK
HHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HLTVVDKANVLASSRLWRQIAQEMAPQYPEVTTDYMFVDNAAMKMLQDPCFFDVMVTENT
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLF
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EYFDLKEEGTLIRKAVDASLDENVRTPEIQVEGGAKYGTKEVGSWIVDYIKKA
HHHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFKIAVLAGDGIGPEISVQGVDVMNAVCEKFGHKVSYEYAICGADAIDKVGDPFPEATF
CCEEEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCHHHH
QACKNADAVLFSAVGDPKFDNDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLVHKSP
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHCCC
LRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNMYTRPEIERILKVAFEYAMKRRK
HHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HLTVVDKANVLASSRLWRQIAQEMAPQYPEVTTDYMFVDNAAMKMLQDPCFFDVMVTENT
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLF
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EYFDLKEEGTLIRKAVDASLDENVRTPEIQVEGGAKYGTKEVGSWIVDYIKKA
HHHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA