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The map label for this gene is cimA [H]

Identifier: 150004552

GI number: 150004552

Start: 2596887

End: 2598434

Strand: Direct

Name: cimA [H]

Synonym: BVU_2007

Alternate gene names: 150004552

Gene position: 2596887-2598434 (Clockwise)

Preceding gene: 150004551

Following gene: 150004553

Centisome position: 50.3

GC content: 41.99

Gene sequence:

>1548_bases
ATGGGAAAAAGCGTCAAAATAGAAATCATGGATACAACACTCCGCGATGGTGAACAAACCAGCGGAGTATCTTTTGTGCC
CCATGAAAAATTGATGATTGCCCGATTGCTATTGGAAGATTTAAAGGTAGACAGGGTAGAGGTTGCCTCGGCCCGTGTAT
CGGATGGCGAATTTGAAGCCGTTAAAATGATATGTGACTGGGCTGCCCGCCGGAATTTGCTTCACAAAGTGGAGGTGCTC
GGATTTGTGGATGGCCGTACTTCTGTAGATTGGATACAACGTACAGGATGCAGGGTAATTAACTTATTGTGTAAAGGTTC
GTTGAAACATTGTACACAGCAGTTGAAGAAAACACCTGAAGAACATATTGAAGATATTGTGAATGTGGTGCGTTATGCCG
ATGAACTGGATATTGCAGTTAATGTATATCTGGAAGATTGGAGTAACGGAATGAAGGATTCTTCTGACTATGTATTCCAG
TTGATGGATGGATTGAAGGATACCAGTATCAAACGGTATATGTTACCGGATACATTGGGTATCTTGAATCCTTTGCAAGT
GATTGAATTCATGCGGAAGATGAAAAAACGTTATCCTCATACTCATTTTGATTTCCATGCGCATAATGATTATGACTTGG
CTGTGAGTAATGTACTTGCTGCTGTGCTGAGTGGGGTTAAAGGACTTCATACTACTATCAATGGATTAGGGGAACGTGCA
GGAAATGCACCGCTTGCCAGTGTTCAAGCTATTCTGAAGGATCATTTTAATGCTTTGACCAATATCGACGAAAGCCGTCT
GAATGATGTGAGCCGAGTGGTGGAATCTTATTCTGGTATTGTTATTCCTGCCAACAAGCCGATTGTGGGTGAGAATGTGT
TTACGCAAGTGGCCGGCGTTCATGCCGACGGTGATAACAAGAATAATCTTTATTGCAATGATTTGTTGCCGGAACGATTC
GGACGTAAACGTGAGTATGCGTTGGGCAAGACTAGTGGCAAAGCCAATATCCGTAAAAATCTGGAGGATTTGGGACTTGA
TTTAGATGAAGAATCCATGCGTAAGGTAACGGAACGTATTATCGAACTGGGTGACAAGAAGGAATTGGTTACTCAGGAAG
ATTTGCCTTATATTGTTTCCGATGTGCTGAAGCATGGAGTGGTAAGCGAAAGTGTAAAACTGAAAAGTTATATTGTGACA
CTGGCGCATGGATTGAAGCCGATGGCTACTGTAAAAATAGAGATTAACGGAAAAGAGTTTGAGGAGAATTCCAGCGGTGA
CGGTCAGTATGACGCTTTTGTGCGTGCATTGCGTAAAATTTATAAAGTGACATTAGGGCGTAAATTCCCTATGTTGACCA
ATTATGCGGTAAGTATTCCTCCTGGGGGCCGTACAGATGCATTTGTGCAAACAGTAATAACTTGGAGTTTTGAAGAAAAA
GTATTCCGTACTCGTGGGCTGGATGCTGACCAGACGGAGGCTGCTATCAAGGCTACCATGAAAATGCTGAATATAATAGA
AAATGAATATGAAAATAATAATGGTTAG

Upstream 100 bases:

>100_bases
AAAGCGAACATTTTGAAATTAATGCATACAAGAAACATTGTTTGATGAACGGATTGGATGATATCGACTTTTTGCTGACC
AACAAAGACAAGATAGAAGC

Downstream 100 bases:

>100_bases
TGATTAGTGGTTAGTGGTTAATGAACAGTGTCGTCAGATTTCATTAACCGCCAACTATTAATCACTGACCGCTAACCATT
AATCACTAAAAAAATGGATT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MGKSVKIEIMDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSDGEFEAVKMICDWAARRNLLHKVEVL
GFVDGRTSVDWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIEDIVNVVRYADELDIAVNVYLEDWSNGMKDSSDYVFQ
LMDGLKDTSIKRYMLPDTLGILNPLQVIEFMRKMKKRYPHTHFDFHAHNDYDLAVSNVLAAVLSGVKGLHTTINGLGERA
GNAPLASVQAILKDHFNALTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNNLYCNDLLPERF
GRKREYALGKTSGKANIRKNLEDLGLDLDEESMRKVTERIIELGDKKELVTQEDLPYIVSDVLKHGVVSESVKLKSYIVT
LAHGLKPMATVKIEINGKEFEENSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWSFEEK
VFRTRGLDADQTEAAIKATMKMLNIIENEYENNNG

Sequences:

>Translated_515_residues
MGKSVKIEIMDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSDGEFEAVKMICDWAARRNLLHKVEVL
GFVDGRTSVDWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIEDIVNVVRYADELDIAVNVYLEDWSNGMKDSSDYVFQ
LMDGLKDTSIKRYMLPDTLGILNPLQVIEFMRKMKKRYPHTHFDFHAHNDYDLAVSNVLAAVLSGVKGLHTTINGLGERA
GNAPLASVQAILKDHFNALTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNNLYCNDLLPERF
GRKREYALGKTSGKANIRKNLEDLGLDLDEESMRKVTERIIELGDKKELVTQEDLPYIVSDVLKHGVVSESVKLKSYIVT
LAHGLKPMATVKIEINGKEFEENSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWSFEEK
VFRTRGLDADQTEAAIKATMKMLNIIENEYENNNG
>Mature_514_residues
GKSVKIEIMDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSDGEFEAVKMICDWAARRNLLHKVEVLG
FVDGRTSVDWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIEDIVNVVRYADELDIAVNVYLEDWSNGMKDSSDYVFQL
MDGLKDTSIKRYMLPDTLGILNPLQVIEFMRKMKKRYPHTHFDFHAHNDYDLAVSNVLAAVLSGVKGLHTTINGLGERAG
NAPLASVQAILKDHFNALTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNNLYCNDLLPERFG
RKREYALGKTSGKANIRKNLEDLGLDLDEESMRKVTERIIELGDKKELVTQEDLPYIVSDVLKHGVVSESVKLKSYIVTL
AHGLKPMATVKIEINGKEFEENSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWSFEEKV
FRTRGLDADQTEAAIKATMKMLNIIENEYENNNG

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=513, Percent_Identity=28.8499025341131, Blast_Score=162, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320071, Length=382, Percent_Identity=26.9633507853403, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320019, Length=370, Percent_Identity=26.7567567567568, Blast_Score=114, Evalue=4e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 57808; Mature: 57677

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSVKIEIMDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSDGEFEA
CCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
VKMICDWAARRNLLHKVEVLGFVDGRTSVDWIQRTGCRVINLLCKGSLKHCTQQLKKTPE
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHH
EHIEDIVNVVRYADELDIAVNVYLEDWSNGMKDSSDYVFQLMDGLKDTSIKRYMLPDTLG
HHHHHHHHHHHHHHCCCEEEEEEEEHHCCCCCCCHHHHHHHHHCCCCCCCHHEECCHHHH
ILNPLQVIEFMRKMKKRYPHTHFDFHAHNDYDLAVSNVLAAVLSGVKGLHTTINGLGERA
HCCHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNAPLASVQAILKDHFNALTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCE
HADGDNKNNLYCNDLLPERFGRKREYALGKTSGKANIRKNLEDLGLDLDEESMRKVTERI
ECCCCCCCCEEECCCCHHHHCCCHHHCCCCCCCCHHHHHCHHHCCCCCCHHHHHHHHHHH
IELGDKKELVTQEDLPYIVSDVLKHGVVSESVKLKSYIVTLAHGLKPMATVKIEINGKEF
HHCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
EENSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWSFEEK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHH
VFRTRGLDADQTEAAIKATMKMLNIIENEYENNNG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GKSVKIEIMDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSDGEFEA
CCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
VKMICDWAARRNLLHKVEVLGFVDGRTSVDWIQRTGCRVINLLCKGSLKHCTQQLKKTPE
HHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHH
EHIEDIVNVVRYADELDIAVNVYLEDWSNGMKDSSDYVFQLMDGLKDTSIKRYMLPDTLG
HHHHHHHHHHHHHHCCCEEEEEEEEHHCCCCCCCHHHHHHHHHCCCCCCCHHEECCHHHH
ILNPLQVIEFMRKMKKRYPHTHFDFHAHNDYDLAVSNVLAAVLSGVKGLHTTINGLGERA
HCCHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNAPLASVQAILKDHFNALTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCE
HADGDNKNNLYCNDLLPERFGRKREYALGKTSGKANIRKNLEDLGLDLDEESMRKVTERI
ECCCCCCCCEEECCCCHHHHCCCHHHCCCCCCCCHHHHHCHHHCCCCCCHHHHHHHHHHH
IELGDKKELVTQEDLPYIVSDVLKHGVVSESVKLKSYIVTLAHGLKPMATVKIEINGKEF
HHCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
EENSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWSFEEK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHCCEEECCCCCCHHHHHHHHHHHHHHHH
VFRTRGLDADQTEAAIKATMKMLNIIENEYENNNG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]