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The map label for this gene is leuC

Identifier: 150004550

GI number: 150004550

Start: 2594916

End: 2596298

Strand: Direct

Name: leuC

Synonym: BVU_2005

Alternate gene names: 150004550

Gene position: 2594916-2596298 (Clockwise)

Preceding gene: 150004549

Following gene: 150004551

Centisome position: 50.26

GC content: 45.26

Gene sequence:

>1383_bases
ATGGCTAACACATTATTTGATAAGATTTGGGACGCCCATGTGGTACAAAAGGTTGAGGAGGGACCTACTCAACTTTATAT
AGACAGGCTTTATTGTCATGAAGTAACTAGTCCGCAGGCTTTTGCGGGACTGCGCGCACGCGGGGTGAAAGTCTTTCGTC
CGGATCATGTATATTGTATGCCGGATCACAATACTCCGACACATGACCAGGATAAACCGATTGAGGATCCGGTTTCCAAA
ACACAGGTGGATACTTTGGCAAAGAATGCCAAAGATTTCGGCTTGGCTCATTTCGGTATGATGGATAAAAGAAATGGTAT
TATTCATGTAGTAGGTCCGGAAAGAGGGCTGACTTTGCCGGGGATGACAATTGTTTGTGGTGACTCGCATACTTCTACCC
ATGGTGCGATGGGAGCTGTTGCTTTCGGTATTGGAACCAGTGAAGTAGAAATGGTGTTGGCTTCACAATGTATCTTACAA
TCTCGTCCGAAAACCATGCGTATCACTATTGATGGTGAACTGGGTAAAGGAGTGACGGCGAAAGACATGGCTCTCTATAT
GATGTCTAAGATGACAACCAGTGGTGCTACTGGATTCTTCGTGGAATATGCCGGTTCGGCTGTCCGTAACTTGAGTATGG
AAGGTCGTCTGACTTTATGTAATCTGAGTATTGAAATGGGGGCACGTGGTGGCATGGTTGCTCCTGATGAAGTGACTTTT
GAATATATAAAAGGCAGGGAACATGCTCCGAAAGGTGTGGATTGGGATAAAGCGGTGTCTCATTGGAAAACATTAAAGAG
TGATGATGATGCTGTATTTGATAAAGAAATTCGTTTTGATGCAGCAGATATTCAGCCGATGATTACATACGGAACGAATC
CAGGAATGGGTATGGGAATCACTGAGCATATTCCGGTAGATGATAAAAGTGCTTCTTTTAAAAAGTCTTTGGACTATATG
GGCTTTCAGCCGGGCGAATCCTTGCTAGGTAAGAAAATTGATTATGTATTTTTGGGTGCTTGCACTAACGGACGAATTGA
GGACTTCCGCGCCTTTACCTCTTTAGTAAGAGGGAAAAAGAAAGCAGATCATGTGACTGCATGGCTGGTTCCGGGATCAT
GGATGGTAGATGCACAGATTCGTGAAGAAGGTTTGGACAAAATCTTGGAGGAGGCAGGTTTTGCCATCCGTCAACCGGGA
TGTTCTGCTTGTCTGGCTATGAATGATGATAAGATTCCGGCCGGAAAGTATTCGGTATCTACTAGTAACCGTAATTTTGA
AGGACGCCAGGGACCGGGTGCTCGCACGTTGCTTGCCAGTCCGCTGGTAGCTGCTGCTGCGGCGGTGACCGGTGTAATAA
CAGATCCTAGAGAACTAATTTAA

Upstream 100 bases:

>100_bases
ACTATGGTTTCGGAGCGAATACAGATATTATCGCTGCATCAGTAGAAGCATATATTGATTGTATCAACAAGTTTAAAAGA
TAATAATACGTAATACAAAA

Downstream 100 bases:

>100_bases
AAATGCGAAGACAATGAAACAGAAATTCAATATCATAACAAGCACTTGCGTACCCCTTCCTTTAGAGAATGTGGACACAG
ACCAAATTATTCCGGCCCGT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MANTLFDKIWDAHVVQKVEEGPTQLYIDRLYCHEVTSPQAFAGLRARGVKVFRPDHVYCMPDHNTPTHDQDKPIEDPVSK
TQVDTLAKNAKDFGLAHFGMMDKRNGIIHVVGPERGLTLPGMTIVCGDSHTSTHGAMGAVAFGIGTSEVEMVLASQCILQ
SRPKTMRITIDGELGKGVTAKDMALYMMSKMTTSGATGFFVEYAGSAVRNLSMEGRLTLCNLSIEMGARGGMVAPDEVTF
EYIKGREHAPKGVDWDKAVSHWKTLKSDDDAVFDKEIRFDAADIQPMITYGTNPGMGMGITEHIPVDDKSASFKKSLDYM
GFQPGESLLGKKIDYVFLGACTNGRIEDFRAFTSLVRGKKKADHVTAWLVPGSWMVDAQIREEGLDKILEEAGFAIRQPG
CSACLAMNDDKIPAGKYSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELI

Sequences:

>Translated_460_residues
MANTLFDKIWDAHVVQKVEEGPTQLYIDRLYCHEVTSPQAFAGLRARGVKVFRPDHVYCMPDHNTPTHDQDKPIEDPVSK
TQVDTLAKNAKDFGLAHFGMMDKRNGIIHVVGPERGLTLPGMTIVCGDSHTSTHGAMGAVAFGIGTSEVEMVLASQCILQ
SRPKTMRITIDGELGKGVTAKDMALYMMSKMTTSGATGFFVEYAGSAVRNLSMEGRLTLCNLSIEMGARGGMVAPDEVTF
EYIKGREHAPKGVDWDKAVSHWKTLKSDDDAVFDKEIRFDAADIQPMITYGTNPGMGMGITEHIPVDDKSASFKKSLDYM
GFQPGESLLGKKIDYVFLGACTNGRIEDFRAFTSLVRGKKKADHVTAWLVPGSWMVDAQIREEGLDKILEEAGFAIRQPG
CSACLAMNDDKIPAGKYSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELI
>Mature_459_residues
ANTLFDKIWDAHVVQKVEEGPTQLYIDRLYCHEVTSPQAFAGLRARGVKVFRPDHVYCMPDHNTPTHDQDKPIEDPVSKT
QVDTLAKNAKDFGLAHFGMMDKRNGIIHVVGPERGLTLPGMTIVCGDSHTSTHGAMGAVAFGIGTSEVEMVLASQCILQS
RPKTMRITIDGELGKGVTAKDMALYMMSKMTTSGATGFFVEYAGSAVRNLSMEGRLTLCNLSIEMGARGGMVAPDEVTFE
YIKGREHAPKGVDWDKAVSHWKTLKSDDDAVFDKEIRFDAADIQPMITYGTNPGMGMGITEHIPVDDKSASFKKSLDYMG
FQPGESLLGKKIDYVFLGACTNGRIEDFRAFTSLVRGKKKADHVTAWLVPGSWMVDAQIREEGLDKILEEAGFAIRQPGC
SACLAMNDDKIPAGKYSVSTSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=472, Percent_Identity=26.0593220338983, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI8659555, Length=439, Percent_Identity=25.2847380410023, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=23.9583333333333, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=53.862660944206, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=26.7029972752044, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI2367097, Length=374, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=470, Percent_Identity=26.3829787234043, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=417, Percent_Identity=26.8585131894484, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25149342, Length=301, Percent_Identity=26.9102990033223, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17568399, Length=433, Percent_Identity=24.2494226327945, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=52.8784648187633, Blast_Score=482, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6322261, Length=442, Percent_Identity=26.0180995475113, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=463, Percent_Identity=26.3498920086393, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320440, Length=482, Percent_Identity=24.0663900414938, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI281365315, Length=466, Percent_Identity=24.4635193133047, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17864292, Length=466, Percent_Identity=24.4635193133047, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28571643, Length=466, Percent_Identity=25.1072961373391, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI161076999, Length=431, Percent_Identity=24.3619489559165, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=24.8648648648649, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24645686, Length=438, Percent_Identity=24.8858447488584, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_BACV8 (A6L1V8)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299294.1
- ProteinModelPortal:   A6L1V8
- SMR:   A6L1V8
- STRING:   A6L1V8
- GeneID:   5302971
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_2005
- NMPDR:   fig|435590.6.peg.1883
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50047; Mature: 49916

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTLFDKIWDAHVVQKVEEGPTQLYIDRLYCHEVTSPQAFAGLRARGVKVFRPDHVYCM
CCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCHHHHHHHHCCEEEECCCEEEEC
PDHNTPTHDQDKPIEDPVSKTQVDTLAKNAKDFGLAHFGMMDKRNGIIHVVGPERGLTLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCHHHHCCEECCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAMGAVAFGIGTSEVEMVLASQCILQSRPKTMRITIDGELGKGVTA
CEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCH
KDMALYMMSKMTTSGATGFFVEYAGSAVRNLSMEGRLTLCNLSIEMGARGGMVAPDEVTF
HHHHHHHHHHHHCCCCCEEEHHHHCHHHHCCCCCCEEEEEEEEEEECCCCCCCCCHHHHH
EYIKGREHAPKGVDWDKAVSHWKTLKSDDDAVFDKEIRFDAADIQPMITYGTNPGMGMGI
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCEEEEECCCCCCCCCC
TEHIPVDDKSASFKKSLDYMGFQPGESLLGKKIDYVFLGACTNGRIEDFRAFTSLVRGKK
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
KADHVTAWLVPGSWMVDAQIREEGLDKILEEAGFAIRQPGCSACLAMNDDKIPAGKYSVS
CCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCEEEE
TSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
ANTLFDKIWDAHVVQKVEEGPTQLYIDRLYCHEVTSPQAFAGLRARGVKVFRPDHVYCM
CCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCHHHHHHHHCCEEEECCCEEEEC
PDHNTPTHDQDKPIEDPVSKTQVDTLAKNAKDFGLAHFGMMDKRNGIIHVVGPERGLTLP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHCCHHHHCCEECCCCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAMGAVAFGIGTSEVEMVLASQCILQSRPKTMRITIDGELGKGVTA
CEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCH
KDMALYMMSKMTTSGATGFFVEYAGSAVRNLSMEGRLTLCNLSIEMGARGGMVAPDEVTF
HHHHHHHHHHHHCCCCCEEEHHHHCHHHHCCCCCCEEEEEEEEEEECCCCCCCCCHHHHH
EYIKGREHAPKGVDWDKAVSHWKTLKSDDDAVFDKEIRFDAADIQPMITYGTNPGMGMGI
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCEEEEECCCCCCCCCC
TEHIPVDDKSASFKKSLDYMGFQPGESLLGKKIDYVFLGACTNGRIEDFRAFTSLVRGKK
CCCCCCCCCCCHHHHHHHHCCCCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCC
KADHVTAWLVPGSWMVDAQIREEGLDKILEEAGFAIRQPGCSACLAMNDDKIPAGKYSVS
CCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCCCCCEEEE
TSNRNFEGRQGPGARTLLASPLVAAAAAVTGVITDPRELI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA