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The map label for this gene is leuA [H]

Identifier: 150004549

GI number: 150004549

Start: 2593402

End: 2594898

Strand: Direct

Name: leuA [H]

Synonym: BVU_2004

Alternate gene names: 150004549

Gene position: 2593402-2594898 (Clockwise)

Preceding gene: 150004548

Following gene: 150004550

Centisome position: 50.23

GC content: 40.68

Gene sequence:

>1497_bases
ATGAGTGACAGATTATTTATTTTTGATACCACTCTTCGTGATGGCGAACAGGTTCCGGGATGTCAGTTGAATACAGTGGA
AAAGATTCAGGTGGCTAAGCAGTTGGAAGCTTTGGGAGTGGATGTGATTGAGGCAGGATTTCCTATTTCAAGTCCGGGTG
ATTTCAATTCAGTAATTGAAATTTCTAAGGCTGTTACTTGGCCAACCATTTGTGCGCTGACTCGTGCAGTTGAAAAAGAT
ATCGACGTAGCTGCTGACGCATTGAAGTTTGCCAAGCACAAACGTATCCATACTGGCATTGGAACTTCAGATTCTCATAT
CAAATATAAATTCAATTCTACTCGTGAAGAAATTATTGAACGTGCTGTGGCTGCTGTGAAATATGCACGTCGTTATGTAG
ATGATGTGGAATTTTATGCGGAAGATGCAGGACGTACGGATAATGAATACTTGGCTCGTGTGGTAGAAGCTGTTATTAAA
GCGGGAGCGACAGTGGTGAATATACCTGATACTACAGGTTATTGCTTGCCTGAAGAGTATGGGGCGAAAATTAAATATTT
GGTAGATCATGTAGATGGTATCGATAAGGCTATTATTTCTACTCATTGTCATAATGATTTGGGTATGGCTACTGCCAATA
CGATTAGTGGTGTGTTAAATGGAGCACGTCAGGTAGAAGTGACTATCAATGGCATTGGAGAACGTGCCGGTAACACTTCC
TTGGAAGAGGTGGCAATGATTGTCAAATGCCATAATGATATTAATATTCAGACAAACATCAATACCCAGAAGATTTATCC
GACTAGTCGTATGGTATCAAGTTTGATGAATATGCCGGTTCAGCCGAACAAGGCAATTGTGGGACGCAATGCTTTTGCAC
ATTCATCCGGTATCCATCAAGATGGTGTTTTAAAAAACGTACAAACATACGAAATTATCGACCCGAAGGATGTGGGCATT
GATGATAATGCTATTGTTTTGACTGCTCGTAGCGGACGTGCTGCGTTAAAACATCGTCTTCATGTTTTGGGAGTAGAACT
GGAACAGGACAAATTGGATAAGGTATATGAGGATTTCTTAAAACTGGCTGATAAGAAGAAAGATATCACGGATGATGATA
TTTTGGTATTGGCAGGTGCCGATAGAAGTGTTAATAACCATATAAAATTGGATTACTTGCAGGTAACAAGCGGAGTGGGC
GTTCGTTCGGTGGCAAGTTTAGGACTGAATATTGCAGGAGAGCACTTTGAAGCTGCTGCCAGTGGAAATGGACCGGTAGA
TGCTGCTATCAAGGCTTTGAAAAAAATTATTGACCGCCACATGGTGTTGAAGGAATTTACGATCCAGGCTATCAGTAAAG
GTAGTGATGATATGGGTAAAGTACATATGCAGGTGGAATATGACGGACAGATGTACTATGGTTTCGGAGCGAATACAGAT
ATTATCGCTGCATCAGTAGAAGCATATATTGATTGTATCAACAAGTTTAAAAGATAA

Upstream 100 bases:

>100_bases
AAGTATGTGTGAACAATGATATACGAAAGCCTTTCTCACTTCCCTGTGTGAAAGGTTTTTTTTATGAATATAATAATAGT
AAAATATAAAATTGAAATTT

Downstream 100 bases:

>100_bases
TAATACGTAATACAAAAATGGCTAACACATTATTTGATAAGATTTGGGACGCCCATGTGGTACAAAAGGTTGAGGAGGGA
CCTACTCAACTTTATATAGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKQLEALGVDVIEAGFPISSPGDFNSVIEISKAVTWPTICALTRAVEKD
IDVAADALKFAKHKRIHTGIGTSDSHIKYKFNSTREEIIERAVAAVKYARRYVDDVEFYAEDAGRTDNEYLARVVEAVIK
AGATVVNIPDTTGYCLPEEYGAKIKYLVDHVDGIDKAIISTHCHNDLGMATANTISGVLNGARQVEVTINGIGERAGNTS
LEEVAMIVKCHNDINIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNVQTYEIIDPKDVGI
DDNAIVLTARSGRAALKHRLHVLGVELEQDKLDKVYEDFLKLADKKKDITDDDILVLAGADRSVNNHIKLDYLQVTSGVG
VRSVASLGLNIAGEHFEAAASGNGPVDAAIKALKKIIDRHMVLKEFTIQAISKGSDDMGKVHMQVEYDGQMYYGFGANTD
IIAASVEAYIDCINKFKR

Sequences:

>Translated_498_residues
MSDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKQLEALGVDVIEAGFPISSPGDFNSVIEISKAVTWPTICALTRAVEKD
IDVAADALKFAKHKRIHTGIGTSDSHIKYKFNSTREEIIERAVAAVKYARRYVDDVEFYAEDAGRTDNEYLARVVEAVIK
AGATVVNIPDTTGYCLPEEYGAKIKYLVDHVDGIDKAIISTHCHNDLGMATANTISGVLNGARQVEVTINGIGERAGNTS
LEEVAMIVKCHNDINIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNVQTYEIIDPKDVGI
DDNAIVLTARSGRAALKHRLHVLGVELEQDKLDKVYEDFLKLADKKKDITDDDILVLAGADRSVNNHIKLDYLQVTSGVG
VRSVASLGLNIAGEHFEAAASGNGPVDAAIKALKKIIDRHMVLKEFTIQAISKGSDDMGKVHMQVEYDGQMYYGFGANTD
IIAASVEAYIDCINKFKR
>Mature_497_residues
SDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKQLEALGVDVIEAGFPISSPGDFNSVIEISKAVTWPTICALTRAVEKDI
DVAADALKFAKHKRIHTGIGTSDSHIKYKFNSTREEIIERAVAAVKYARRYVDDVEFYAEDAGRTDNEYLARVVEAVIKA
GATVVNIPDTTGYCLPEEYGAKIKYLVDHVDGIDKAIISTHCHNDLGMATANTISGVLNGARQVEVTINGIGERAGNTSL
EEVAMIVKCHNDINIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQDGVLKNVQTYEIIDPKDVGID
DNAIVLTARSGRAALKHRLHVLGVELEQDKLDKVYEDFLKLADKKKDITDDDILVLAGADRSVNNHIKLDYLQVTSGVGV
RSVASLGLNIAGEHFEAAASGNGPVDAAIKALKKIIDRHMVLKEFTIQAISKGSDDMGKVHMQVEYDGQMYYGFGANTDI
IAASVEAYIDCINKFKR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=501, Percent_Identity=51.0978043912176, Blast_Score=489, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6324225, Length=532, Percent_Identity=27.2556390977444, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324682, Length=540, Percent_Identity=26.8518518518519, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320019, Length=375, Percent_Identity=29.6, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320071, Length=368, Percent_Identity=29.6195652173913, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 54448; Mature: 54316

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01181 RIBOSOMAL_S21 ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKQLEALGVDVIEAGFPISSPGDFNSVIE
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHH
ISKAVTWPTICALTRAVEKDIDVAADALKFAKHKRIHTGIGTSDSHIKYKFNSTREEIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHH
RAVAAVKYARRYVDDVEFYAEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPEEY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GAKIKYLVDHVDGIDKAIISTHCHNDLGMATANTISGVLNGARQVEVTINGIGERAGNTS
CCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
LEEVAMIVKCHNDINIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQ
HHHHHHHHEECCCCEEEECCCCCEECCHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCCC
DGVLKNVQTYEIIDPKDVGIDDNAIVLTARSGRAALKHRLHVLGVELEQDKLDKVYEDFL
CCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHEEEEECHHHHHHHHHHHH
KLADKKKDITDDDILVLAGADRSVNNHIKLDYLQVTSGVGVRSVASLGLNIAGEHFEAAA
HHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCHHEECC
SGNGPVDAAIKALKKIIDRHMVLKEFTIQAISKGSDDMGKVHMQVEYDGQMYYGFGANTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECCCCCC
IIAASVEAYIDCINKFKR
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDRLFIFDTTLRDGEQVPGCQLNTVEKIQVAKQLEALGVDVIEAGFPISSPGDFNSVIE
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHH
ISKAVTWPTICALTRAVEKDIDVAADALKFAKHKRIHTGIGTSDSHIKYKFNSTREEIIE
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHH
RAVAAVKYARRYVDDVEFYAEDAGRTDNEYLARVVEAVIKAGATVVNIPDTTGYCLPEEY
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GAKIKYLVDHVDGIDKAIISTHCHNDLGMATANTISGVLNGARQVEVTINGIGERAGNTS
CCHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
LEEVAMIVKCHNDINIQTNINTQKIYPTSRMVSSLMNMPVQPNKAIVGRNAFAHSSGIHQ
HHHHHHHHEECCCCEEEECCCCCEECCHHHHHHHHHCCCCCCCCEEECCCCHHCCCCCCC
DGVLKNVQTYEIIDPKDVGIDDNAIVLTARSGRAALKHRLHVLGVELEQDKLDKVYEDFL
CCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHEEEEECHHHHHHHHHHHH
KLADKKKDITDDDILVLAGADRSVNNHIKLDYLQVTSGVGVRSVASLGLNIAGEHFEAAA
HHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCHHEECC
SGNGPVDAAIKALKKIIDRHMVLKEFTIQAISKGSDDMGKVHMQVEYDGQMYYGFGANTD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCEEEEECCCCCC
IIAASVEAYIDCINKFKR
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA