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The map label for this gene is lipA

Identifier: 150004540

GI number: 150004540

Start: 2575692

End: 2576546

Strand: Reverse

Name: lipA

Synonym: BVU_1990

Alternate gene names: 150004540

Gene position: 2576546-2575692 (Counterclockwise)

Preceding gene: 150004541

Following gene: 150004539

Centisome position: 49.9

GC content: 42.92

Gene sequence:

>855_bases
ATGAATCACGTTAAAAAACCAGACTGGCTTAAGATAAACATAGGGGCAAATGCTCGATATACCGAAACCAAACATATTGT
AGATTCCCACAAACTACATACCATTTGCAGTAGCGGACGATGTCCCAATATGGGAGAATGCTGGGGAAAAGGTACTGCCA
CTTTTATGATAGGTGGCGAAATTTGTACCCGTTCCTGCAAATTTTGCAACACACAGACCGGACGGCCTCTTCCGTTAGAC
CCTGAAGAACCCACCCATGTAGCAGAGTCCATTCAACTCATGAAATTATCTCATGCCGTTATTACCTCTGTAGACCGTGA
TGATCTAGATGATTTGGGTGCTGCCCATTGGGCTAAAACGATTTCAGAAATCAAGCGCTTGAATCCGGAAACCACAACCG
AAGTTTTAATCCCTGATTTTCAAGGAAAGAGCGAACTGATTCAGCTAGTCATTGACGCCAAACCAGACATCATCTCACAC
AATATGGAAACCGTACGCCGCATCAGTCCCCTGGTACGAAGCGCAGCCAACTATGCAACCAGTTTAAAGGTCATCAAACA
AATAGCCAATAATGGTATTACAGCTAAATCCGGTATTATGGTAGGTTTGGGAGAACGCCCGGAAGAAGTAGAAGAAGTCA
TGGACGATTTATTGGCACAAGGCTGCAAGATACTTACTATTGGCCAATATCTACAACCTACCCACCGCCATTATCCGGTA
GCGGAATATATTACTCCCGATCAATTTAAACAGTATAGAACAATTGGATTAAAGAAAGGATTCCGAGAAGTGGAAAGCGC
ACCTTTGGTACGTTCCTCCTATCATGCAGAAAAACATATAAAATTTAAAGGGTAA

Upstream 100 bases:

>100_bases
AAAAAACCATTCACCACAGATTATACAATTTTACATAAAAACCGAATGAAAACAATTTGCATTGGTATAATCTGTGGTGA
ATTATAAATCTAATATCATC

Downstream 100 bases:

>100_bases
CGAGTAAACCAACTTTATATCTTTTTTGTTATAAAGATATAAATAGAGATAGAGAGAATGAAATCGTTACTGGAATACAA
GAATTTAATCATGTCCACAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNHVKKPDWLKINIGANARYTETKHIVDSHKLHTICSSGRCPNMGECWGKGTATFMIGGEICTRSCKFCNTQTGRPLPLD
PEEPTHVAESIQLMKLSHAVITSVDRDDLDDLGAAHWAKTISEIKRLNPETTTEVLIPDFQGKSELIQLVIDAKPDIISH
NMETVRRISPLVRSAANYATSLKVIKQIANNGITAKSGIMVGLGERPEEVEEVMDDLLAQGCKILTIGQYLQPTHRHYPV
AEYITPDQFKQYRTIGLKKGFREVESAPLVRSSYHAEKHIKFKG

Sequences:

>Translated_284_residues
MNHVKKPDWLKINIGANARYTETKHIVDSHKLHTICSSGRCPNMGECWGKGTATFMIGGEICTRSCKFCNTQTGRPLPLD
PEEPTHVAESIQLMKLSHAVITSVDRDDLDDLGAAHWAKTISEIKRLNPETTTEVLIPDFQGKSELIQLVIDAKPDIISH
NMETVRRISPLVRSAANYATSLKVIKQIANNGITAKSGIMVGLGERPEEVEEVMDDLLAQGCKILTIGQYLQPTHRHYPV
AEYITPDQFKQYRTIGLKKGFREVESAPLVRSSYHAEKHIKFKG
>Mature_284_residues
MNHVKKPDWLKINIGANARYTETKHIVDSHKLHTICSSGRCPNMGECWGKGTATFMIGGEICTRSCKFCNTQTGRPLPLD
PEEPTHVAESIQLMKLSHAVITSVDRDDLDDLGAAHWAKTISEIKRLNPETTTEVLIPDFQGKSELIQLVIDAKPDIISH
NMETVRRISPLVRSAANYATSLKVIKQIANNGITAKSGIMVGLGERPEEVEEVMDDLLAQGCKILTIGQYLQPTHRHYPV
AEYITPDQFKQYRTIGLKKGFREVESAPLVRSSYHAEKHIKFKG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=44.1696113074205, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=42.914979757085, Blast_Score=201, Evalue=5e-52,
Organism=Escherichia coli, GI1786846, Length=282, Percent_Identity=44.6808510638298, Blast_Score=257, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=42.1602787456446, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=281, Percent_Identity=41.6370106761566, Blast_Score=228, Evalue=9e-61,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=43.4628975265018, Blast_Score=243, Evalue=9e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_BACV8 (A6L1U8)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299284.1
- ProteinModelPortal:   A6L1U8
- STRING:   A6L1U8
- GeneID:   5302956
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1990
- NMPDR:   fig|435590.6.peg.1871
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 31726; Mature: 31726

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHVKKPDWLKINIGANARYTETKHIVDSHKLHTICSSGRCPNMGECWGKGTATFMIGGE
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECHH
ICTRSCKFCNTQTGRPLPLDPEEPTHVAESIQLMKLSHAVITSVDRDDLDDLGAAHWAKT
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ISEIKRLNPETTTEVLIPDFQGKSELIQLVIDAKPDIISHNMETVRRISPLVRSAANYAT
HHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
SLKVIKQIANNGITAKSGIMVGLGERPEEVEEVMDDLLAQGCKILTIGQYLQPTHRHYPV
HHHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECHHHCHHHCCCCH
AEYITPDQFKQYRTIGLKKGFREVESAPLVRSSYHAEKHIKFKG
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHCCEEECC
>Mature Secondary Structure
MNHVKKPDWLKINIGANARYTETKHIVDSHKLHTICSSGRCPNMGECWGKGTATFMIGGE
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECHH
ICTRSCKFCNTQTGRPLPLDPEEPTHVAESIQLMKLSHAVITSVDRDDLDDLGAAHWAKT
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ISEIKRLNPETTTEVLIPDFQGKSELIQLVIDAKPDIISHNMETVRRISPLVRSAANYAT
HHHHHHCCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHH
SLKVIKQIANNGITAKSGIMVGLGERPEEVEEVMDDLLAQGCKILTIGQYLQPTHRHYPV
HHHHHHHHHCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECHHHCHHHCCCCH
AEYITPDQFKQYRTIGLKKGFREVESAPLVRSSYHAEKHIKFKG
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA