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The map label for this gene is thyA

Identifier: 150004500

GI number: 150004500

Start: 2528357

End: 2529151

Strand: Reverse

Name: thyA

Synonym: BVU_1950

Alternate gene names: 150004500

Gene position: 2529151-2528357 (Counterclockwise)

Preceding gene: 150004501

Following gene: 150004499

Centisome position: 48.98

GC content: 42.77

Gene sequence:

>795_bases
ATGAAACAATATTTAGACCTATTAAACCGTATCCTGACCGAAGGTGTTCACAAGGAGGATCGCACCGGTACAGGAACCAT
CAGCGTATTCGGTCATCAAATGCGCTTCAACTTGGAAGAGGGTTTCCCTTTGGTCACCACCAAAAAGCTGCATTTAAAAT
CCATCATTCACGAACTTCTGTGGTTTCTGCAAGGGGATACCAATGTGAAATACCTGCAAGACAACGGGGTACGCATCTGG
AATGAATGGGCCGATGCAAACGGAGACCTAGGACATATCTACGGCTATCAATGGCGTTCCTGGCCAGATTACAATGGAGG
CTTTATTGACCAAATTTCCGAAGCAATAGAAACGATCAAGCATAATCCGGATTCACGACGCATCATTGTCAGTGCATGGA
ATGTAGCCGATTTAAATAACATGAATCTTCCCCCTTGCCATGCTTTTTTTCAATTCTACGTTGCCAACGGACGTTTAAGC
CTACAACTTTACCAACGCAGTGCAGATACATTCTTGGGAGTACCTTTTAATATCGCCTCCTACGCATTGTTGCTGCAAAT
GGTAGCACAAGTTACCGGATTACAGACCGGTGATTTTGTTCATACACTAGGTGACACACATATCTATACCAACCACTTGG
AACAAGTAAAACTTCAATTGAGCCGTGAACCCCGTCCCTTACCTCAAATGAAAATCAACCCGGATATCAAGGATATATTC
AGTTTTAAATATGAAGATTTTGAATTGGTGAATTATGATCCATGGCCACACATTGCGGGTAAAGTATCTGTTTAG

Upstream 100 bases:

>100_bases
ATGCAGCCTGAAATGGTGAAAACTGAAATAGAACTAGGGTCATGCTCGGAAAAAGAAAGATTTTGTGTACTTTTGCAATC
TATAAAATGAATATCCGAAC

Downstream 100 bases:

>100_bases
CAGTTATGGTGAATAGGCTGCACAAGCTTATTCATCACTTACCACTAATATCACTAAACATTCACCACTAACCACTCATC
ACTATGAATATTTCAATTAT

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLLNRILTEGVHKEDRTGTGTISVFGHQMRFNLEEGFPLVTTKKLHLKSIIHELLWFLQGDTNVKYLQDNGVRIW
NEWADANGDLGHIYGYQWRSWPDYNGGFIDQISEAIETIKHNPDSRRIIVSAWNVADLNNMNLPPCHAFFQFYVANGRLS
LQLYQRSADTFLGVPFNIASYALLLQMVAQVTGLQTGDFVHTLGDTHIYTNHLEQVKLQLSREPRPLPQMKINPDIKDIF
SFKYEDFELVNYDPWPHIAGKVSV

Sequences:

>Translated_264_residues
MKQYLDLLNRILTEGVHKEDRTGTGTISVFGHQMRFNLEEGFPLVTTKKLHLKSIIHELLWFLQGDTNVKYLQDNGVRIW
NEWADANGDLGHIYGYQWRSWPDYNGGFIDQISEAIETIKHNPDSRRIIVSAWNVADLNNMNLPPCHAFFQFYVANGRLS
LQLYQRSADTFLGVPFNIASYALLLQMVAQVTGLQTGDFVHTLGDTHIYTNHLEQVKLQLSREPRPLPQMKINPDIKDIF
SFKYEDFELVNYDPWPHIAGKVSV
>Mature_264_residues
MKQYLDLLNRILTEGVHKEDRTGTGTISVFGHQMRFNLEEGFPLVTTKKLHLKSIIHELLWFLQGDTNVKYLQDNGVRIW
NEWADANGDLGHIYGYQWRSWPDYNGGFIDQISEAIETIKHNPDSRRIIVSAWNVADLNNMNLPPCHAFFQFYVANGRLS
LQLYQRSADTFLGVPFNIASYALLLQMVAQVTGLQTGDFVHTLGDTHIYTNHLEQVKLQLSREPRPLPQMKINPDIKDIF
SFKYEDFELVNYDPWPHIAGKVSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=52.4822695035461, Blast_Score=300, Evalue=6e-82,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=64.7727272727273, Blast_Score=378, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.9964664310954, Blast_Score=270, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=42.1232876712329, Blast_Score=218, Evalue=6e-58,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=51.5901060070671, Blast_Score=295, Evalue=2e-80,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_BACV8 (A6L1Q8)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299244.1
- ProteinModelPortal:   A6L1Q8
- SMR:   A6L1Q8
- STRING:   A6L1Q8
- GeneID:   5302916
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1950
- NMPDR:   fig|435590.6.peg.1833
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30479; Mature: 30479

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLLNRILTEGVHKEDRTGTGTISVFGHQMRFNLEEGFPLVTTKKLHLKSIIHELL
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGDTNVKYLQDNGVRIWNEWADANGDLGHIYGYQWRSWPDYNGGFIDQISEAIETIK
HHHCCCCCEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
HNPDSRRIIVSAWNVADLNNMNLPPCHAFFQFYVANGRLSLQLYQRSADTFLGVPFNIAS
CCCCCCEEEEEEECEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEECCCHHHHH
YALLLQMVAQVTGLQTGDFVHTLGDTHIYTNHLEQVKLQLSREPRPLPQMKINPDIKDIF
HHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
SFKYEDFELVNYDPWPHIAGKVSV
HCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLLNRILTEGVHKEDRTGTGTISVFGHQMRFNLEEGFPLVTTKKLHLKSIIHELL
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGDTNVKYLQDNGVRIWNEWADANGDLGHIYGYQWRSWPDYNGGFIDQISEAIETIK
HHHCCCCCEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
HNPDSRRIIVSAWNVADLNNMNLPPCHAFFQFYVANGRLSLQLYQRSADTFLGVPFNIAS
CCCCCCEEEEEEECEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEECCCHHHHH
YALLLQMVAQVTGLQTGDFVHTLGDTHIYTNHLEQVKLQLSREPRPLPQMKINPDIKDIF
HHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
SFKYEDFELVNYDPWPHIAGKVSV
HCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA