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The map label for this gene is folA [H]

Identifier: 150004499

GI number: 150004499

Start: 2527779

End: 2528273

Strand: Reverse

Name: folA [H]

Synonym: BVU_1949

Alternate gene names: 150004499

Gene position: 2528273-2527779 (Counterclockwise)

Preceding gene: 150004500

Following gene: 150004497

Centisome position: 48.97

GC content: 38.38

Gene sequence:

>495_bases
ATGAATATTTCAATTATAGTAGCAATAGCACGCAACCACGGCATTGGATTTGAAAATAAATTATTATATTGGCTTCCCAA
CGATTTGAAACGTTTTAAAGCTTTAACAACCGGACATACTATCATCATGGGACGCAAAACCTTTGAATCACTTCCCAAAG
GAGCACTTCCCAATCGCCGGAATATTGTATTAAGCCGCCGGGAAGGAGCAGTCTTTGCCGGAGCTGAATGTTTCCCTGCA
TTGGAAATCGCTTTATCCCACTGCAAAGAAGAAGAAGAAATATTTATTATAGGCGGTGCAAGCTTGTATAAAGAGGCAAT
GCATATAGCCCAACAATTATATATCACATTTATTGATGACACATCCAAGGAAGCTGATGCTTATTTTCCCGAAATAAAGA
AAGAGGAATGGAAAGAAACAGGCAGAGAAGATCACCCGACAGATGAAAAACATCTCTACCCGTATAGTTTTATTAATTAT
AGCCGGATAAAGTAA

Upstream 100 bases:

>100_bases
GTAAAGTATCTGTTTAGCAGTTATGGTGAATAGGCTGCACAAGCTTATTCATCACTTACCACTAATATCACTAAACATTC
ACCACTAACCACTCATCACT

Downstream 100 bases:

>100_bases
CGATTAATCTTCATCAGCTAAATCGAGGATAGGCATTTGTCTCTTGATAGCCTCATTAAAAGATATAATAGTTTCTGAAC
GAGCCAAGCCCAATGGTTGC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MNISIIVAIARNHGIGFENKLLYWLPNDLKRFKALTTGHTIIMGRKTFESLPKGALPNRRNIVLSRREGAVFAGAECFPA
LEIALSHCKEEEEIFIIGGASLYKEAMHIAQQLYITFIDDTSKEADAYFPEIKKEEWKETGREDHPTDEKHLYPYSFINY
SRIK

Sequences:

>Translated_164_residues
MNISIIVAIARNHGIGFENKLLYWLPNDLKRFKALTTGHTIIMGRKTFESLPKGALPNRRNIVLSRREGAVFAGAECFPA
LEIALSHCKEEEEIFIIGGASLYKEAMHIAQQLYITFIDDTSKEADAYFPEIKKEEWKETGREDHPTDEKHLYPYSFINY
SRIK
>Mature_164_residues
MNISIIVAIARNHGIGFENKLLYWLPNDLKRFKALTTGHTIIMGRKTFESLPKGALPNRRNIVLSRREGAVFAGAECFPA
LEIALSHCKEEEEIFIIGGASLYKEAMHIAQQLYITFIDDTSKEADAYFPEIKKEEWKETGREDHPTDEKHLYPYSFINY
SRIK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=157, Percent_Identity=34.3949044585987, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1786233, Length=155, Percent_Identity=36.1290322580645, Blast_Score=100, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6324810, Length=174, Percent_Identity=30.4597701149425, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI116008026, Length=148, Percent_Identity=35.1351351351351, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24647458, Length=148, Percent_Identity=35.1351351351351, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18849; Mature: 18849

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISIIVAIARNHGIGFENKLLYWLPNDLKRFKALTTGHTIIMGRKTFESLPKGALPNRR
CCEEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCC
NIVLSRREGAVFAGAECFPALEIALSHCKEEEEIFIIGGASLYKEAMHIAQQLYITFIDD
CEEEEECCCCEEECCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHEEEEECC
TSKEADAYFPEIKKEEWKETGREDHPTDEKHLYPYSFINYSRIK
CCCCCHHHCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCEECCC
>Mature Secondary Structure
MNISIIVAIARNHGIGFENKLLYWLPNDLKRFKALTTGHTIIMGRKTFESLPKGALPNRR
CCEEEEEEEECCCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEECHHHHHCCCCCCCCCC
NIVLSRREGAVFAGAECFPALEIALSHCKEEEEIFIIGGASLYKEAMHIAQQLYITFIDD
CEEEEECCCCEEECCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHEEEEECC
TSKEADAYFPEIKKEEWKETGREDHPTDEKHLYPYSFINYSRIK
CCCCCHHHCCCCCHHHHHHCCCCCCCCCCCEECCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]