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The map label for this gene is birA [H]

Identifier: 150004430

GI number: 150004430

Start: 2452933

End: 2453700

Strand: Reverse

Name: birA [H]

Synonym: BVU_1877

Alternate gene names: 150004430

Gene position: 2453700-2452933 (Counterclockwise)

Preceding gene: 150004431

Following gene: 150004429

Centisome position: 47.52

GC content: 39.58

Gene sequence:

>768_bases
ATGATGAGATCCCCTAACTGGTCAAGCAAAATCTTAAAAGAAGAAACCGTAACCTCAACAAATGACCGACTTGCAGAACT
TTGCCAGGAAAAAGAAATCAAGGAATTCACCACGCTTATGGCAGAATATCAAACCGCCGGAAAAGGGCAACGTGGAAATT
CATGGGAATCAGAATATGGCAAGAATCTGACATTCAGCACTGTATTTTACCCCCAAACAATCGCTCCCGCCAGTCAGTTC
ATTTTATCTATGACGGTAGCTTCTGCCATTTGTACCGCATTGGCCCATTATGTCCATGCAGATTGCCTGCAAATAAAATG
GCCCAATGATATCTATTGGAAAGACAAAAAAATAGCCGGCATATTGATTGAAAATGATTTAACAGGAAGCCAAATTTCAC
AGAGCATTATTGGGATAGGGATCAATATTAACCAGGAAGAATTTCATTCATCTGCTCCCAACCCTGTTTCATTAAGACAA
ATTACTCAAAAGAAAACAGACAGAATGGAAGTTCTGAACAGCGTATTGGAACATATCATAGATCTCTATTCCCGGATAGA
AAACAGAGAAACCGATATCATTACCAAAATTCAGGAATACTACCTGAAAGCACAATACCGCAAGAAAGGTTATCACCCCT
ATTGCGATGCAAAAGGAGAGTTTACTGCCAAACTGATCCGTGTGGAGCCCGACGGACATTTGATTCTGAAAGATAAAAAC
GGTTCTCTTAGAAAATATGCTTTCAAAGAAGTTAAATACCTCCTTTAG

Upstream 100 bases:

>100_bases
TCCTTACAAGGTAACATTGACGACAAACTTATTTTATCACTGATAGACCACTCATACGAAGAGGTTATCAAAAAATTCAC
CCGTAAACTTAAAAAAGAAT

Downstream 100 bases:

>100_bases
TTGTTCTTCTCTTCAGGAACCATCAACATTCCTCCAGTGGCATGACGTTCTGTCGATTGATACACAGATTCCCCCCTCAT
AATCGGTTGGGATAACGTAG

Product: putative biotin-(acetyl-CoA carboxylase) synthetase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MMRSPNWSSKILKEETVTSTNDRLAELCQEKEIKEFTTLMAEYQTAGKGQRGNSWESEYGKNLTFSTVFYPQTIAPASQF
ILSMTVASAICTALAHYVHADCLQIKWPNDIYWKDKKIAGILIENDLTGSQISQSIIGIGININQEEFHSSAPNPVSLRQ
ITQKKTDRMEVLNSVLEHIIDLYSRIENRETDIITKIQEYYLKAQYRKKGYHPYCDAKGEFTAKLIRVEPDGHLILKDKN
GSLRKYAFKEVKYLL

Sequences:

>Translated_255_residues
MMRSPNWSSKILKEETVTSTNDRLAELCQEKEIKEFTTLMAEYQTAGKGQRGNSWESEYGKNLTFSTVFYPQTIAPASQF
ILSMTVASAICTALAHYVHADCLQIKWPNDIYWKDKKIAGILIENDLTGSQISQSIIGIGININQEEFHSSAPNPVSLRQ
ITQKKTDRMEVLNSVLEHIIDLYSRIENRETDIITKIQEYYLKAQYRKKGYHPYCDAKGEFTAKLIRVEPDGHLILKDKN
GSLRKYAFKEVKYLL
>Mature_255_residues
MMRSPNWSSKILKEETVTSTNDRLAELCQEKEIKEFTTLMAEYQTAGKGQRGNSWESEYGKNLTFSTVFYPQTIAPASQF
ILSMTVASAICTALAHYVHADCLQIKWPNDIYWKDKKIAGILIENDLTGSQISQSIIGIGININQEEFHSSAPNPVSLRQ
ITQKKTDRMEVLNSVLEHIIDLYSRIENRETDIITKIQEYYLKAQYRKKGYHPYCDAKGEFTAKLIRVEPDGHLILKDKN
GSLRKYAFKEVKYLL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=141, Percent_Identity=38.2978723404255, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 29269; Mature: 29269

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRSPNWSSKILKEETVTSTNDRLAELCQEKEIKEFTTLMAEYQTAGKGQRGNSWESEYG
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KNLTFSTVFYPQTIAPASQFILSMTVASAICTALAHYVHADCLQIKWPNDIYWKDKKIAG
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCEEE
ILIENDLTGSQISQSIIGIGININQEEFHSSAPNPVSLRQITQKKTDRMEVLNSVLEHII
EEEECCCCHHHHHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLYSRIENRETDIITKIQEYYLKAQYRKKGYHPYCDAKGEFTAKLIRVEPDGHLILKDKN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEECCC
GSLRKYAFKEVKYLL
CCHHHHHHHHHHHCC
>Mature Secondary Structure
MMRSPNWSSKILKEETVTSTNDRLAELCQEKEIKEFTTLMAEYQTAGKGQRGNSWESEYG
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KNLTFSTVFYPQTIAPASQFILSMTVASAICTALAHYVHADCLQIKWPNDIYWKDKKIAG
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCEEE
ILIENDLTGSQISQSIIGIGININQEEFHSSAPNPVSLRQITQKKTDRMEVLNSVLEHII
EEEECCCCHHHHHHHHHEECCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLYSRIENRETDIITKIQEYYLKAQYRKKGYHPYCDAKGEFTAKLIRVEPDGHLILKDKN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCEEEEECCC
GSLRKYAFKEVKYLL
CCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]