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The map label for this gene is purH [H]

Identifier: 150004375

GI number: 150004375

Start: 2365763

End: 2367286

Strand: Direct

Name: purH [H]

Synonym: BVU_1821

Alternate gene names: 150004375

Gene position: 2365763-2367286 (Clockwise)

Preceding gene: 150004372

Following gene: 150004376

Centisome position: 45.82

GC content: 42.19

Gene sequence:

>1524_bases
ATGTCAGAGGCTAAGAGAATTAAAACCGCATTGGTGTCGGTTTATCACAAAGAAGGTTTGGACGAGATTATAACCAAACT
ACACGAAGAAGGAGTAGAGTTCCTGTCAACAGGAGGTACAAGACAGTTTATCGAATCTTTAGGTTTCCCTTGCAAAGCGG
TGGAGGATCTTACTACTTATCCCTCTATTTTGGGCGGACGTGTGAAGACACTGCATCCTAAAGTCTTTGGAGGTATTTTA
TGCCGTCGTGGTTTGGAACAAGATATGCAACAGATAGAAAAATATGAAATACCCGAAATAGATTTGGTCATCGTTGACCT
GTATCCGTTTGAGGCAACTGTGGCTTCGGGGGCCGAAGAACAAGCTATCATCGAAAAAATTGATATAGGTGGTATTTCTT
TGATTCGTGCTGCTGCTAAGAACTTTAATGATGTGGTGATTATCGCTAGTCAGGCACAATATAAGCCGTTCCGTGATATG
TTGCTGGAACATGGCGCTACTACTTCACGCGAGGAACGCCGTTGGTTCGCAAAAGAAGCGTTTGCTGTTTCTTCTCATTA
TGATTCTGCTATCTTTAACTATTTTGATGGTGGTGAAGGTTCGGCTTTCCGTTGCGCGGTGGAAGAACAAAAACAACTTC
GTTATGGTGAAAATCCTCATCAGAAAGGTTATTTCTATGGAAATCTGGATGCGATGTTCGACCAGATTCATGGAAAAGAA
ATTTCCTATAACAATTTGTTGGATATCAATGCGGCCGTAGATTTGATTGATGAATTTGATGAGGTGACATTTGCCATTCT
GAAGCATAATAATGCCTGTGGATTAGCTTCGCGCCCTACTGTGCTGGAAGCATGGAAAGATGCGTTGGCAGGCGATCCGG
TGTCGGCTTTCGGAGGTGTGTTGGTTACTAATGCCGTGATTGATAAAGAAACAGCGGAAGAAATCAATAAACTGTTCTTT
GAGGTAATTATTGCTCCGGATTATGATGTAGATGCATTAGAGATTCTGGGACAGAAGAAGAACCGTATTATTTTGGTTCG
TAAGGAAGCTAAATTGCCGAAGAAACAATTCCGTTCTTTATTGAATGGGGTTTTGGTTCAGGAAAGAGACCTGGATGTGG
AAACTCCTGCCGACTTGAAGCAGGTTACTGAAAAAGCTCCTACGGCGACTGAAATAGAAGATATGTTATTTGCAAATAAG
ATTGTGAAGAATAGTAAAAGTAATGCTATTGTACTGGCCAAGAACAAACAACTGCTGGCTAGCGGTGTAGGTCAGACTTC
ACGTGTGGATGCATTGAAACAAGCCATTGAAAAAGCAAAATCATTTGAGTTTGATTTGAATGGCGCAGTGATGGCCAGTG
ATGCTTTCTTCCCTTTCCCCGATTGTGTAGAGATAGCGGATAAGGAGGGTGTGAAAGCTGTTATCCAGCCGGGAGGCTCT
GTAAAAGACGAGTTGACTTTCCAATACTGCAATGAACATGGCGTAGCTATGGTCATTACAGGGATTCGTCACTTTAAACA
CTAA

Upstream 100 bases:

>100_bases
GAAACAAATATGAGTGGATAAATAATATTTCACGTTTATGTGTGGAATTTATAGAGTGAAAATTTATACCTTTGTAGCCC
AAATACTTAATCTATAAAAA

Downstream 100 bases:

>100_bases
GATCATTGCAATTTACCATTTGCGGCTACAATCGGACTGTTTATACAGCATTGTTCAGGTAAATGGTAAATTGCAAATGT
CAATTGTAAATTGCTAAATT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSEAKRIKTALVSVYHKEGLDEIITKLHEEGVEFLSTGGTRQFIESLGFPCKAVEDLTTYPSILGGRVKTLHPKVFGGIL
CRRGLEQDMQQIEKYEIPEIDLVIVDLYPFEATVASGAEEQAIIEKIDIGGISLIRAAAKNFNDVVIIASQAQYKPFRDM
LLEHGATTSREERRWFAKEAFAVSSHYDSAIFNYFDGGEGSAFRCAVEEQKQLRYGENPHQKGYFYGNLDAMFDQIHGKE
ISYNNLLDINAAVDLIDEFDEVTFAILKHNNACGLASRPTVLEAWKDALAGDPVSAFGGVLVTNAVIDKETAEEINKLFF
EVIIAPDYDVDALEILGQKKNRIILVRKEAKLPKKQFRSLLNGVLVQERDLDVETPADLKQVTEKAPTATEIEDMLFANK
IVKNSKSNAIVLAKNKQLLASGVGQTSRVDALKQAIEKAKSFEFDLNGAVMASDAFFPFPDCVEIADKEGVKAVIQPGGS
VKDELTFQYCNEHGVAMVITGIRHFKH

Sequences:

>Translated_507_residues
MSEAKRIKTALVSVYHKEGLDEIITKLHEEGVEFLSTGGTRQFIESLGFPCKAVEDLTTYPSILGGRVKTLHPKVFGGIL
CRRGLEQDMQQIEKYEIPEIDLVIVDLYPFEATVASGAEEQAIIEKIDIGGISLIRAAAKNFNDVVIIASQAQYKPFRDM
LLEHGATTSREERRWFAKEAFAVSSHYDSAIFNYFDGGEGSAFRCAVEEQKQLRYGENPHQKGYFYGNLDAMFDQIHGKE
ISYNNLLDINAAVDLIDEFDEVTFAILKHNNACGLASRPTVLEAWKDALAGDPVSAFGGVLVTNAVIDKETAEEINKLFF
EVIIAPDYDVDALEILGQKKNRIILVRKEAKLPKKQFRSLLNGVLVQERDLDVETPADLKQVTEKAPTATEIEDMLFANK
IVKNSKSNAIVLAKNKQLLASGVGQTSRVDALKQAIEKAKSFEFDLNGAVMASDAFFPFPDCVEIADKEGVKAVIQPGGS
VKDELTFQYCNEHGVAMVITGIRHFKH
>Mature_506_residues
SEAKRIKTALVSVYHKEGLDEIITKLHEEGVEFLSTGGTRQFIESLGFPCKAVEDLTTYPSILGGRVKTLHPKVFGGILC
RRGLEQDMQQIEKYEIPEIDLVIVDLYPFEATVASGAEEQAIIEKIDIGGISLIRAAAKNFNDVVIIASQAQYKPFRDML
LEHGATTSREERRWFAKEAFAVSSHYDSAIFNYFDGGEGSAFRCAVEEQKQLRYGENPHQKGYFYGNLDAMFDQIHGKEI
SYNNLLDINAAVDLIDEFDEVTFAILKHNNACGLASRPTVLEAWKDALAGDPVSAFGGVLVTNAVIDKETAEEINKLFFE
VIIAPDYDVDALEILGQKKNRIILVRKEAKLPKKQFRSLLNGVLVQERDLDVETPADLKQVTEKAPTATEIEDMLFANKI
VKNSKSNAIVLAKNKQLLASGVGQTSRVDALKQAIEKAKSFEFDLNGAVMASDAFFPFPDCVEIADKEGVKAVIQPGGSV
KDELTFQYCNEHGVAMVITGIRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=465, Percent_Identity=31.8279569892473, Blast_Score=191, Evalue=1e-48,
Organism=Escherichia coli, GI1790439, Length=532, Percent_Identity=40.4135338345865, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71985564, Length=603, Percent_Identity=30.514096185738, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI71985574, Length=336, Percent_Identity=25.8928571428571, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985556, Length=121, Percent_Identity=38.0165289256198, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323056, Length=473, Percent_Identity=33.4038054968288, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6323768, Length=481, Percent_Identity=32.6403326403326, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24649832, Length=466, Percent_Identity=31.7596566523605, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56234; Mature: 56102

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAKRIKTALVSVYHKEGLDEIITKLHEEGVEFLSTGGTRQFIESLGFPCKAVEDLTTY
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHH
PSILGGRVKTLHPKVFGGILCRRGLEQDMQQIEKYEIPEIDLVIVDLYPFEATVASGAEE
HHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHCCCHH
QAIIEKIDIGGISLIRAAAKNFNDVVIIASQAQYKPFRDMLLEHGATTSREERRWFAKEA
HHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
FAVSSHYDSAIFNYFDGGEGSAFRCAVEEQKQLRYGENPHQKGYFYGNLDAMFDQIHGKE
HHHHHHHCHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCC
ISYNNLLDINAAVDLIDEFDEVTFAILKHNNACGLASRPTVLEAWKDALAGDPVSAFGGV
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCH
LVTNAVIDKETAEEINKLFFEVIIAPDYDVDALEILGQKKNRIILVRKEAKLPKKQFRSL
HHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
LNGVLVQERDLDVETPADLKQVTEKAPTATEIEDMLFANKIVKNSKSNAIVLAKNKQLLA
HHHHEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
SGVGQTSRVDALKQAIEKAKSFEFDLNGAVMASDAFFPFPDCVEIADKEGVKAVIQPGGS
HCCCCHHHHHHHHHHHHHHHCCEECCCCEEEECCCCCCCCHHHHHHCCCCCEEEECCCCC
VKDELTFQYCNEHGVAMVITGIRHFKH
CCHHHHHHHHCCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
SEAKRIKTALVSVYHKEGLDEIITKLHEEGVEFLSTGGTRQFIESLGFPCKAVEDLTTY
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHH
PSILGGRVKTLHPKVFGGILCRRGLEQDMQQIEKYEIPEIDLVIVDLYPFEATVASGAEE
HHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHCCCHH
QAIIEKIDIGGISLIRAAAKNFNDVVIIASQAQYKPFRDMLLEHGATTSREERRWFAKEA
HHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHHH
FAVSSHYDSAIFNYFDGGEGSAFRCAVEEQKQLRYGENPHQKGYFYGNLDAMFDQIHGKE
HHHHHHHCHHHHHCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCC
ISYNNLLDINAAVDLIDEFDEVTFAILKHNNACGLASRPTVLEAWKDALAGDPVSAFGGV
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCH
LVTNAVIDKETAEEINKLFFEVIIAPDYDVDALEILGQKKNRIILVRKEAKLPKKQFRSL
HHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
LNGVLVQERDLDVETPADLKQVTEKAPTATEIEDMLFANKIVKNSKSNAIVLAKNKQLLA
HHHHEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
SGVGQTSRVDALKQAIEKAKSFEFDLNGAVMASDAFFPFPDCVEIADKEGVKAVIQPGGS
HCCCCHHHHHHHHHHHHHHHCCEECCCCEEEECCCCCCCCHHHHHHCCCCCEEEECCCCC
VKDELTFQYCNEHGVAMVITGIRHFKH
CCHHHHHHHHCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]