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The map label for this gene is nuoB

Identifier: 150004312

GI number: 150004312

Start: 2255592

End: 2256188

Strand: Reverse

Name: nuoB

Synonym: BVU_1758

Alternate gene names: 150004312

Gene position: 2256188-2255592 (Counterclockwise)

Preceding gene: 150004313

Following gene: 150004311

Centisome position: 43.7

GC content: 46.23

Gene sequence:

>597_bases
ATGGAAGTAAAGAAACCAACCATAAAGTCTATTCCTTATGAAGACTTCAAAGACAATGAGTATTTGGAAAACATGGTCAA
GCAGCTCAATGAAGGCGGTACCAAAGTACTGGTGGGCTGTTTGGATGACCTTATCAACTGGGGACGCAGCAATTCGTTGT
GGCCGCTTACATTTGCAACCAGTTGTTGTGGAATCGAGTTTATGGCAGTGGGTGCTGCCCGGTATGATTTCGCCCGCTTT
GGGTTTGAAGTAGCCCGTGCCAGTCCGCGCCAGGCAGATATGATCATGGTAGCAGGAACTATCACACACAAGATGGCTCC
GGTACTAAAACGTTTGTATGACCAGATGGCGGATCCTAAATACGTCATTGCAGTAGGCGGATGCGCCATTAGCGGCGGTC
CTTTTAAAAAATCATATCACGTATTGAACGGAGTAGACAAAATTCTTCCCGTTGACGTATATATTCCCGGATGTCCGCCC
CGACCTGAAGCCTTGTTGTACGGCATGATACAATTGCAACGTAAAGTAAAACTGGAAAAATTCTTCGGCGGAGTGAACCG
CAAAGAAAATGAACCACAAAATCCCGAACAAGCATGA

Upstream 100 bases:

>100_bases
TGGTACAAGACCTAGGAGTGTACGGTCTGTTTAGCATCCTTTTCTTTTTAGTTATATTAGTTTTAGGTCTGGCATATGCC
TGGAAGAAAGGAGCGTTGGA

Downstream 100 bases:

>100_bases
GTGAAAGAATAGAAATACCTGCCGAAAAGCTACATGAAGAAATGCTGAAATTGCGGCAGGGTAAACATATGGATTTTCTA
CGCAGCCTTACCGGAATGGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MEVKKPTIKSIPYEDFKDNEYLENMVKQLNEGGTKVLVGCLDDLINWGRSNSLWPLTFATSCCGIEFMAVGAARYDFARF
GFEVARASPRQADMIMVAGTITHKMAPVLKRLYDQMADPKYVIAVGGCAISGGPFKKSYHVLNGVDKILPVDVYIPGCPP
RPEALLYGMIQLQRKVKLEKFFGGVNRKENEPQNPEQA

Sequences:

>Translated_198_residues
MEVKKPTIKSIPYEDFKDNEYLENMVKQLNEGGTKVLVGCLDDLINWGRSNSLWPLTFATSCCGIEFMAVGAARYDFARF
GFEVARASPRQADMIMVAGTITHKMAPVLKRLYDQMADPKYVIAVGGCAISGGPFKKSYHVLNGVDKILPVDVYIPGCPP
RPEALLYGMIQLQRKVKLEKFFGGVNRKENEPQNPEQA
>Mature_198_residues
MEVKKPTIKSIPYEDFKDNEYLENMVKQLNEGGTKVLVGCLDDLINWGRSNSLWPLTFATSCCGIEFMAVGAARYDFARF
GFEVARASPRQADMIMVAGTITHKMAPVLKRLYDQMADPKYVIAVGGCAISGGPFKKSYHVLNGVDKILPVDVYIPGCPP
RPEALLYGMIQLQRKVKLEKFFGGVNRKENEPQNPEQA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=144, Percent_Identity=58.3333333333333, Blast_Score=192, Evalue=2e-49,
Organism=Escherichia coli, GI1788624, Length=150, Percent_Identity=55.3333333333333, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1789074, Length=107, Percent_Identity=46.7289719626168, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1788834, Length=141, Percent_Identity=36.1702127659575, Blast_Score=91, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=140, Percent_Identity=55.7142857142857, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI18859983, Length=148, Percent_Identity=57.4324324324324, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24642371, Length=148, Percent_Identity=57.4324324324324, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=59.1240875912409, Blast_Score=186, Evalue=8e-48,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_BACV8 (A6L170)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001299056.1
- ProteinModelPortal:   A6L170
- SMR:   A6L170
- STRING:   A6L170
- GeneID:   5302724
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1758
- NMPDR:   fig|435590.6.peg.1655
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   CCAIEFM
- ProtClustDB:   PRK14816
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 22081; Mature: 22081

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKKPTIKSIPYEDFKDNEYLENMVKQLNEGGTKVLVGCLDDLINWGRSNSLWPLTFAT
CCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
SCCGIEFMAVGAARYDFARFGFEVARASPRQADMIMVAGTITHKMAPVLKRLYDQMADPK
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
YVIAVGGCAISGGPFKKSYHVLNGVDKILPVDVYIPGCPPRPEALLYGMIQLQRKVKLEK
EEEEECCEEECCCCCCHHHHHHHCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
FFGGVNRKENEPQNPEQA
HHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEVKKPTIKSIPYEDFKDNEYLENMVKQLNEGGTKVLVGCLDDLINWGRSNSLWPLTFAT
CCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
SCCGIEFMAVGAARYDFARFGFEVARASPRQADMIMVAGTITHKMAPVLKRLYDQMADPK
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
YVIAVGGCAISGGPFKKSYHVLNGVDKILPVDVYIPGCPPRPEALLYGMIQLQRKVKLEK
EEEEECCEEECCCCCCHHHHHHHCHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
FFGGVNRKENEPQNPEQA
HHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA