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The map label for this gene is purH [H]

Identifier: 150004252

GI number: 150004252

Start: 2181262

End: 2182437

Strand: Reverse

Name: purH [H]

Synonym: BVU_1693

Alternate gene names: 150004252

Gene position: 2182437-2181262 (Counterclockwise)

Preceding gene: 150004254

Following gene: 150004250

Centisome position: 42.27

GC content: 46.34

Gene sequence:

>1176_bases
ATGGCAAAGGAATTAGAATTAAAGTACGGCTGCAATCCCAACCAAAAACCGTCACGCATCTTCATGCAAGAAGGGGAATT
GCCTATCGAAGTATTAAACGGACGTCCCGGATACATCAATTTTCTGGACGCTTTCAACAGTTGGCAACTGGTCAAAGAAT
TGAAAGAAGCTACCGGACTACCCGCAGCAGCCTCCTTCAAGCATGTCAGTCCGGCAGGAGCCGCAGTAGGAATCGAGTTG
AATGACACCTTGAAGAAAATTTATTTTGTGAATGACTTGCCACTTACCCCATTGGCTACCGCATATGCCCGTGCTCGTGG
CGCTGACCGCATGTCTTCTTACGGAGACTTTATCGCATTGAGCGATACTTGCGACGAAGCTACCGCGCGCCTCATCAACC
GTGAAGTTTCAGACGGCGTCATAGCTCCCGATTACACACCGGAAGCGCTGGAAATCCTGAAAAACAAACGCAAGGGAACA
TACAATGTAATTAAAATAGACCCAGCTTACCGCCCTGCACCTATTGAACACAAAGATGTATTCGGCATTACTTTTGAACA
GGGCCGCAACGAAATCAGACTGGACGAAAGCCTGCTGACTCATATTCCAACTAAAAGCCAGCATTTTCCAGACGAAGCAA
AACGCGATTTGATTATTGCCCTTATCACCTTAAAATATACACAAAGTAATTCTGTTTGTTATGTAAAAGACGGGCAGGCT
ATCGGCATCGGTGCCGGCCAGCAAAGCCGCATCCACTGTACACGTCTTGCCGGTAATAAAGCGGATATATGGTATTTGCG
CCAACATCCTAAAGTAATGAATCTGCCGTGGGTAGAAAAAATCCGCCGTGCAGATCGTGATAATACAATTGATGTCTATA
TCAGTGACGATCATGAAGATGTTCTGGCTGACGGCATCTGGCAACAATTCTTCACTAAAAAACCGGAAGTGCTGACTCGC
GAAGAAAAGCGCGCGTGGCTGGATACCATGAAGGGGGTATCATTGGGTTCGGATGCTTTCTTCCCATTTGGTGACAATAT
AGAACGTGCGCATAAGAGCGGAGTGGAATACATAGCACAAGCTGGAGGCTCTGTACGTGATGACCATGTAATTGACACTT
GTGACAAATACGGCATCACCATGGCATTCACTGGTGTTCGTTTATTCCATCATTAA

Upstream 100 bases:

>100_bases
ATTGCCTGCAACCAAAAACAGTTCTTATAACCTGTCTTCCCCATTTATTTTGTACCTTTGCCACACATTGAAAATCTATA
AACCGAAATATTATAAAACT

Downstream 100 bases:

>100_bases
TTATTCATACTCAGTGCAAATTGCAACACGGATTTACAGATAAAAAGCAAACCATACTTAGCCTGTAAGATCCGTGTTTA
CAATCCATATAAAAAATCGT

Product: 5-aminoimidazole-4-carboxamide ribonucleotide transformylase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAKELELKYGCNPNQKPSRIFMQEGELPIEVLNGRPGYINFLDAFNSWQLVKELKEATGLPAAASFKHVSPAGAAVGIEL
NDTLKKIYFVNDLPLTPLATAYARARGADRMSSYGDFIALSDTCDEATARLINREVSDGVIAPDYTPEALEILKNKRKGT
YNVIKIDPAYRPAPIEHKDVFGITFEQGRNEIRLDESLLTHIPTKSQHFPDEAKRDLIIALITLKYTQSNSVCYVKDGQA
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWVEKIRRADRDNTIDVYISDDHEDVLADGIWQQFFTKKPEVLTR
EEKRAWLDTMKGVSLGSDAFFPFGDNIERAHKSGVEYIAQAGGSVRDDHVIDTCDKYGITMAFTGVRLFHH

Sequences:

>Translated_391_residues
MAKELELKYGCNPNQKPSRIFMQEGELPIEVLNGRPGYINFLDAFNSWQLVKELKEATGLPAAASFKHVSPAGAAVGIEL
NDTLKKIYFVNDLPLTPLATAYARARGADRMSSYGDFIALSDTCDEATARLINREVSDGVIAPDYTPEALEILKNKRKGT
YNVIKIDPAYRPAPIEHKDVFGITFEQGRNEIRLDESLLTHIPTKSQHFPDEAKRDLIIALITLKYTQSNSVCYVKDGQA
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWVEKIRRADRDNTIDVYISDDHEDVLADGIWQQFFTKKPEVLTR
EEKRAWLDTMKGVSLGSDAFFPFGDNIERAHKSGVEYIAQAGGSVRDDHVIDTCDKYGITMAFTGVRLFHH
>Mature_390_residues
AKELELKYGCNPNQKPSRIFMQEGELPIEVLNGRPGYINFLDAFNSWQLVKELKEATGLPAAASFKHVSPAGAAVGIELN
DTLKKIYFVNDLPLTPLATAYARARGADRMSSYGDFIALSDTCDEATARLINREVSDGVIAPDYTPEALEILKNKRKGTY
NVIKIDPAYRPAPIEHKDVFGITFEQGRNEIRLDESLLTHIPTKSQHFPDEAKRDLIIALITLKYTQSNSVCYVKDGQAI
GIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWVEKIRRADRDNTIDVYISDDHEDVLADGIWQQFFTKKPEVLTRE
EKRAWLDTMKGVSLGSDAFFPFGDNIERAHKSGVEYIAQAGGSVRDDHVIDTCDKYGITMAFTGVRLFHH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=393, Percent_Identity=54.70737913486, Blast_Score=456, Evalue=1e-128,
Organism=Escherichia coli, GI1790439, Length=268, Percent_Identity=29.8507462686567, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985564, Length=394, Percent_Identity=53.8071065989848, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71985556, Length=420, Percent_Identity=48.3333333333333, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71985574, Length=298, Percent_Identity=56.7114093959732, Blast_Score=326, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6323056, Length=390, Percent_Identity=54.1025641025641, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6323768, Length=393, Percent_Identity=52.4173027989822, Blast_Score=430, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24649832, Length=390, Percent_Identity=57.6923076923077, Blast_Score=474, Evalue=1e-134,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 43916; Mature: 43785

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKELELKYGCNPNQKPSRIFMQEGELPIEVLNGRPGYINFLDAFNSWQLVKELKEATGL
CCCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCEEHHHHHCCHHHHHHHHHHHCCC
PAAASFKHVSPAGAAVGIELNDTLKKIYFVNDLPLTPLATAYARARGADRMSSYGDFIAL
CCCCCCCCCCCCCCEEEEEEHHCEEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCEEEE
SDTCDEATARLINREVSDGVIAPDYTPEALEILKNKRKGTYNVIKIDPAYRPAPIEHKDV
CCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCE
FGITFEQGRNEIRLDESLLTHIPTKSQHFPDEAKRDLIIALITLKYTQSNSVCYVKDGQA
EEEEHHCCCCEEEECHHHHHHCCCCCCCCCCHHHHCEEEEEEEEEEECCCCEEEEECCCE
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWVEKIRRADRDNTIDVYISDDHED
EEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHH
VLADGIWQQFFTKKPEVLTREEKRAWLDTMKGVSLGSDAFFPFGDNIERAHKSGVEYIAQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGGSVRDDHVIDTCDKYGITMAFTGVRLFHH
CCCCCCCCCHHHHHHHCCEEEEEECEEECCC
>Mature Secondary Structure 
AKELELKYGCNPNQKPSRIFMQEGELPIEVLNGRPGYINFLDAFNSWQLVKELKEATGL
CCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCCCEEHHHHHCCHHHHHHHHHHHCCC
PAAASFKHVSPAGAAVGIELNDTLKKIYFVNDLPLTPLATAYARARGADRMSSYGDFIAL
CCCCCCCCCCCCCCEEEEEEHHCEEEEEEECCCCCCHHHHHHHHHCCHHHHHCCCCEEEE
SDTCDEATARLINREVSDGVIAPDYTPEALEILKNKRKGTYNVIKIDPAYRPAPIEHKDV
CCCHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCE
FGITFEQGRNEIRLDESLLTHIPTKSQHFPDEAKRDLIIALITLKYTQSNSVCYVKDGQA
EEEEHHCCCCEEEECHHHHHHCCCCCCCCCCHHHHCEEEEEEEEEEECCCCEEEEECCCE
IGIGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNLPWVEKIRRADRDNTIDVYISDDHED
EEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHH
VLADGIWQQFFTKKPEVLTREEKRAWLDTMKGVSLGSDAFFPFGDNIERAHKSGVEYIAQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGGSVRDDHVIDTCDKYGITMAFTGVRLFHH
CCCCCCCCCHHHHHHHCCEEEEEECEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA