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The map label for this gene is ispF

Identifier: 150004198

GI number: 150004198

Start: 2123307

End: 2123789

Strand: Direct

Name: ispF

Synonym: BVU_1639

Alternate gene names: 150004198

Gene position: 2123307-2123789 (Clockwise)

Preceding gene: 150004197

Following gene: 150004200

Centisome position: 41.12

GC content: 43.69

Gene sequence:

>483_bases
ATGAAAATAAGAGTAGGATTTGGATTCGATGTGCACCAACTGGTAACCGGTCGTGAACTATGGCTTGGAGGAATAAAATT
TGAACATGAATTGGGGCTATTGGGACACTCGGATGCCGATGTGTTGATTCATGCCATTTGTGACGCTTTGCTGGGAGCTG
CCAATATGCGTGATATAGGTTATCATTTTCCTGATACTGCGGGTGAATTCAAGAATATTGATAGCAAGATTTTATTGGCT
GAAACGGTGGACCTGATTGCTGCAAAAGGATATAAAGTAGGTAATGTGGACGCAACGATTTGTGCTGAACGTCCTAAATT
GAAAGCGCGGATTCCGGAAATGCAGTTGGTACTTGCTCATTTGATGAGGATTGATGTGGATGATGTTTCCGTAAAGGCTA
CCACTACAGAGAAACTGGGATTTACGGGCAGGGAGGAGGGCATTTCCGCTTATGCCACTGTATTGATAGAGAAAGCGGAA
TGA

Upstream 100 bases:

>100_bases
TGCTCTATACGGGGACTCCTGTGGGAGTAGGGCCAGTCGGCATAGGACAGCATTTGCAGGGGTATTTGGAAGGTGAGAAA
TTATTAGATTTTTATGTAAG

Downstream 100 bases:

>100_bases
TTTGTTGCGCTTTATTGGGAAATATCTTCTGGCAAGATCTTGCTGGAAGATTATAGGATAAGGCCGAATGCTAGTAATAC
GCCGAATAGCAGCATATTGC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKIRVGFGFDVHQLVTGRELWLGGIKFEHELGLLGHSDADVLIHAICDALLGAANMRDIGYHFPDTAGEFKNIDSKILLA
ETVDLIAAKGYKVGNVDATICAERPKLKARIPEMQLVLAHLMRIDVDDVSVKATTTEKLGFTGREEGISAYATVLIEKAE

Sequences:

>Translated_160_residues
MKIRVGFGFDVHQLVTGRELWLGGIKFEHELGLLGHSDADVLIHAICDALLGAANMRDIGYHFPDTAGEFKNIDSKILLA
ETVDLIAAKGYKVGNVDATICAERPKLKARIPEMQLVLAHLMRIDVDDVSVKATTTEKLGFTGREEGISAYATVLIEKAE
>Mature_160_residues
MKIRVGFGFDVHQLVTGRELWLGGIKFEHELGLLGHSDADVLIHAICDALLGAANMRDIGYHFPDTAGEFKNIDSKILLA
ETVDLIAAKGYKVGNVDATICAERPKLKARIPEMQLVLAHLMRIDVDDVSVKATTTEKLGFTGREEGISAYATVLIEKAE

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=157, Percent_Identity=52.8662420382166, Blast_Score=151, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_BACV8 (A6L0V6)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001298942.1
- ProteinModelPortal:   A6L0V6
- SMR:   A6L0V6
- STRING:   A6L0V6
- GeneID:   5302605
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_1639
- NMPDR:   fig|435590.6.peg.1543
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17474; Mature: 17474

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRVGFGFDVHQLVTGRELWLGGIKFEHELGLLGHSDADVLIHAICDALLGAANMRDIG
CEEEEECCCCHHHHHCCCCEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YHFPDTAGEFKNIDSKILLAETVDLIAAKGYKVGNVDATICAERPKLKARIPEMQLVLAH
CCCCCCCCHHHCCCCEEEHHHHHHHHHHCCEEECCCCEEEECCCCCCHHCCCHHHHHHHH
LMRIDVDDVSVKATTTEKLGFTGREEGISAYATVLIEKAE
HHHCCCCCCEEEEECHHHCCCCCCHHHHHEEEEEEEEECC
>Mature Secondary Structure
MKIRVGFGFDVHQLVTGRELWLGGIKFEHELGLLGHSDADVLIHAICDALLGAANMRDIG
CEEEEECCCCHHHHHCCCCEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
YHFPDTAGEFKNIDSKILLAETVDLIAAKGYKVGNVDATICAERPKLKARIPEMQLVLAH
CCCCCCCCHHHCCCCEEEHHHHHHHHHHCCEEECCCCEEEECCCCCCHHCCCHHHHHHHH
LMRIDVDDVSVKATTTEKLGFTGREEGISAYATVLIEKAE
HHHCCCCCCEEEEECHHHCCCCCCHHHHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA