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The map label for this gene is prfB [H]

Identifier: 150004050

GI number: 150004050

Start: 1968072

End: 1969187

Strand: Direct

Name: prfB [H]

Synonym: BVU_1485

Alternate gene names: 150004050

Gene position: 1968072-1969187 (Clockwise)

Preceding gene: 150004049

Following gene: 150004051

Centisome position: 38.12

GC content: 45.7

Gene sequence:

>1116_bases
ATGATAACAATAGAGCAACTGAAAGACGTAAAAGAACGTACTGCGGCACTTGAAAGATATCTGGACATAGAAAATAAACT
GGTTCAGGTGGAAGAAGAACAACTGCGTACGCAGGCGCCCGGTTTTTGGGATGATGCCAAGAAAGCAGAAGCACAAATGA
GGAAGGTGAAAGATCTGCAAAAATGGATCGACGGTTACCGTGAGGTAAAGACGATGGCAGATGAACTGGAATTGGCTTTT
GACTTTTGTAAAGATGATTTGGTTACCGAAGAAGAAGTGGATGCAGCGTATCAAAAGGCTGTCACTGCGGTGGAGGCATT
GGAACTGAAGAATATGCTTCGCCAGGAGGCCGACCAAATGGATTGTGTATTGAAAATTAATTGCGGTGCCGGTGGTACTG
AAAGTCAGGATTGGGCTTCCATGCTGATGCGTATGTATATGCGTTGGGCGGAGACCAATGGCTATAAAGTGAGCGTGGCT
AACCTTCAGGATGGGGATGAGGCCGGAATCAAGACGGTGACTATGAATATTGAGGGCAGTTTTGCATATGGTTATCTGAA
AGGTGAGAATGGAGTCCACCGCTTGGTGCGCGTGTCTCCTTATAATGCTCAGGGGAAACGGATGACTTCTTTTGCTTCTG
TGTTTGTAACGCCGTTGGTGGATGATAGTATTGAAGTGACAATTGAACCTGCCCGTATGTCTTGGGATACTTTCCGTTCG
GGAGGGGCCGGCGGACAGAATGTGAACAAGGTGGAATCAGGAGTACGTCTGCGTTATCAATATAAAGATCCGTATACCGG
TGAGGAAGAGGAAATCTTGATTGAGAATACTGAAACCCGTGACCAGCCGAAGAATAAGGAAAATGCGATGAGACAGTTGC
GTTCAATTTTATATGATAAGGAATTGCAGCACCGCATGGAAGAACAGGCCAAGGTGGAGGCAGGCAAGAAGAAGATTGAA
TGGGGATCACAGATACGCAGTTATGTCTTTGATGACCGTCGTGTGAAGGATCATCGTACTAATTTTCAAACTTCGGATGT
GAACGGAGTGATGGATGGAAAGATTGATGGCTTTATCAAGGCATACTTGATGGAGTTTTCCGGTTCGGAGAATTAG

Upstream 100 bases:

>100_bases
CAAGAACTATGCTGCCGAAATAGAAAAGATGTACGAAGAATAATGGGAAAATGAATAGATTTCCACTATCTTTGCACCTT
GAAATGCAAAAATTCTCAAG

Downstream 100 bases:

>100_bases
TAAATTCTTCGTAATTTATTTGTTTTCTTCTAGAAACTTTGTACTTTTGGGATATTCAAAAGAGATGGTTTAATCTTAAA
AATGAAATACTTATGGGAAA

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MITIEQLKDVKERTAALERYLDIENKLVQVEEEQLRTQAPGFWDDAKKAEAQMRKVKDLQKWIDGYREVKTMADELELAF
DFCKDDLVTEEEVDAAYQKAVTAVEALELKNMLRQEADQMDCVLKINCGAGGTESQDWASMLMRMYMRWAETNGYKVSVA
NLQDGDEAGIKTVTMNIEGSFAYGYLKGENGVHRLVRVSPYNAQGKRMTSFASVFVTPLVDDSIEVTIEPARMSWDTFRS
GGAGGQNVNKVESGVRLRYQYKDPYTGEEEEILIENTETRDQPKNKENAMRQLRSILYDKELQHRMEEQAKVEAGKKKIE
WGSQIRSYVFDDRRVKDHRTNFQTSDVNGVMDGKIDGFIKAYLMEFSGSEN

Sequences:

>Translated_371_residues
MITIEQLKDVKERTAALERYLDIENKLVQVEEEQLRTQAPGFWDDAKKAEAQMRKVKDLQKWIDGYREVKTMADELELAF
DFCKDDLVTEEEVDAAYQKAVTAVEALELKNMLRQEADQMDCVLKINCGAGGTESQDWASMLMRMYMRWAETNGYKVSVA
NLQDGDEAGIKTVTMNIEGSFAYGYLKGENGVHRLVRVSPYNAQGKRMTSFASVFVTPLVDDSIEVTIEPARMSWDTFRS
GGAGGQNVNKVESGVRLRYQYKDPYTGEEEEILIENTETRDQPKNKENAMRQLRSILYDKELQHRMEEQAKVEAGKKKIE
WGSQIRSYVFDDRRVKDHRTNFQTSDVNGVMDGKIDGFIKAYLMEFSGSEN
>Mature_371_residues
MITIEQLKDVKERTAALERYLDIENKLVQVEEEQLRTQAPGFWDDAKKAEAQMRKVKDLQKWIDGYREVKTMADELELAF
DFCKDDLVTEEEVDAAYQKAVTAVEALELKNMLRQEADQMDCVLKINCGAGGTESQDWASMLMRMYMRWAETNGYKVSVA
NLQDGDEAGIKTVTMNIEGSFAYGYLKGENGVHRLVRVSPYNAQGKRMTSFASVFVTPLVDDSIEVTIEPARMSWDTFRS
GGAGGQNVNKVESGVRLRYQYKDPYTGEEEEILIENTETRDQPKNKENAMRQLRSILYDKELQHRMEEQAKVEAGKKKIE
WGSQIRSYVFDDRRVKDHRTNFQTSDVNGVMDGKIDGFIKAYLMEFSGSEN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=244, Percent_Identity=34.0163934426229, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI34577120, Length=263, Percent_Identity=31.5589353612167, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=32.8859060402685, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI2367172, Length=364, Percent_Identity=41.7582417582418, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI1787462, Length=254, Percent_Identity=34.6456692913386, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17542784, Length=325, Percent_Identity=27.3846153846154, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=28.7066246056782, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19921226, Length=256, Percent_Identity=33.59375, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42517; Mature: 42517

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIEQLKDVKERTAALERYLDIENKLVQVEEEQLRTQAPGFWDDAKKAEAQMRKVKDLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
KWIDGYREVKTMADELELAFDFCKDDLVTEEEVDAAYQKAVTAVEALELKNMLRQEADQM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DCVLKINCGAGGTESQDWASMLMRMYMRWAETNGYKVSVANLQDGDEAGIKTVTMNIEGS
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCC
FAYGYLKGENGVHRLVRVSPYNAQGKRMTSFASVFVTPLVDDSIEVTIEPARMSWDTFRS
EEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCC
GGAGGQNVNKVESGVRLRYQYKDPYTGEEEEILIENTETRDQPKNKENAMRQLRSILYDK
CCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
ELQHRMEEQAKVEAGKKKIEWGSQIRSYVFDDRRVKDHRTNFQTSDVNGVMDGKIDGFIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
AYLMEFSGSEN
HHHHHHCCCCC
>Mature Secondary Structure
MITIEQLKDVKERTAALERYLDIENKLVQVEEEQLRTQAPGFWDDAKKAEAQMRKVKDLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
KWIDGYREVKTMADELELAFDFCKDDLVTEEEVDAAYQKAVTAVEALELKNMLRQEADQM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DCVLKINCGAGGTESQDWASMLMRMYMRWAETNGYKVSVANLQDGDEAGIKTVTMNIEGS
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCC
FAYGYLKGENGVHRLVRVSPYNAQGKRMTSFASVFVTPLVDDSIEVTIEPARMSWDTFRS
EEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHCC
GGAGGQNVNKVESGVRLRYQYKDPYTGEEEEILIENTETRDQPKNKENAMRQLRSILYDK
CCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
ELQHRMEEQAKVEAGKKKIEWGSQIRSYVFDDRRVKDHRTNFQTSDVNGVMDGKIDGFIK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
AYLMEFSGSEN
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA