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The map label for this gene is pflD [H]

Identifier: 150003987

GI number: 150003987

Start: 1897393

End: 1899774

Strand: Reverse

Name: pflD [H]

Synonym: BVU_1421

Alternate gene names: 150003987

Gene position: 1899774-1897393 (Counterclockwise)

Preceding gene: 150003988

Following gene: 150003986

Centisome position: 36.79

GC content: 46.47

Gene sequence:

>2382_bases
ATGAAAACAACAATCAACGGTATGACAGACCGTATCCGACGCCTACGTCAAGAATCTTTTGATACACAACCTTCTTTGTC
CATAGAACGTGCTCTGATAGAAACCGAATTTTACAAAGAAAACGAAGGAAAGTATCCAATTCCTATTCTAAGAGCACTGA
ACTTCCTTGAAATCTGTAAACGGAAAACTATATACATAGGTGAAGACGAACTGATTGTAGGTGAACGTGGACCACGCCCC
AAAGCCGTATCGACTTTCCCTGAACTGACCTGTCACAGCGTGGAAGACCTTCATGTATTGAACACCCGCGAATTGCAGCG
TTACACCATCAGCCAGGAAGATATAGACACCTATGAGCGCGAGGTAATTCCTTATTGGAAAGGGCGCACCCAACGCGAAC
GCATCTTCAATCATGTTCCCAAAGAATGGAAAGAAGCTTACGAAGTAGGAATGTTCACTGAATTCATGGAACAACGAGCC
CCCGGCCATACTTCTCTGGACGGAAAGGTTTATCAACATGGCATGCTGGATTTGAAAGAACGTATTGCACATGAGTTGAA
AAATCTGGATTTTATGAATGATCCAGAAGCAACCGACAAACAAGAAGAACTGACCGCCATGTCCATCTCCTGTGATGCCG
CTATCATTTTTGCCGAACGCCATGCCGAACTGGCCGAAAAGATGGCTGGACAAGAAACCGATCCGAAACGCAAAGAGGAA
CTGAAAAAAATAGCGGAAATCTGCCGATGGGTTCCTGCACATGCCCCCCGTACTTATTGGGAGGCCATACAAATGTACTG
GTTTGTCCATCTCGGCACCATTACAGAGCTGAACGGATGGGACGCCATGAATCCAGGACATTTTGACCAGCACCTCGCTC
CTTTCTATGAAAAAGACCTGGAAGAAGGAATACTGACCCGTGCAGAGGCCAAAGAACTGATGTCATGTTTCTTTATCAAA
GTAAATAATCAGACAGCCCCACCCAAAGTTGGCATCACCGCCAAAGAAAGCGGTACCTACAATGACTTTACCAATCTGAA
TATCGGTGGCATCAAACGTGATGGTAGCAACGGAGTCAGCGAAGTATCCTATATCATGCTCGAAACAGTAGATGATCTTC
ACCTGTTACAACCCGGAAGCGCCCTGCACATCAGTGTATGCACCCCGGAGCGTTTCCTGCGCGAAGGCTGCAAAGTAATC
CGTAAGGGCTATGGCTACCCATCTGTATTCAATCCCGACACATACGTCATGGAACTGATGCGACAGGGAAAAACACCGGA
GGACGCTCGTGAAGGCGGATGTAGCGGATGCATAGAAGTAGGCGCTTTCGGAAAAGAAGCTTACATACTGACCGGTTACC
TTAACGTACCCAAGATATTGGAAGTCACCTTGCACAACGGAACAGATCCTGTATCAGGCAAGAAAGTAGGCTTGGTAACA
GGAGATCCCTGTACCTTCCGGAGTTATGAAGCACTTTATGACGCATTTCTTAAACAAATCCATTATTTTGTAGATATGAA
AGTACGCGTCAGCAACTACATTGACCGTATGTTCGCCAAATACGCTCCCGCCACTTTCCTGTCTTTGTTCATTGATGACT
GTATCGCCAAAGGAAAAGATTATTATAACTGCGGACCGCGTTATAACACAAGCTATATTCAATGCACCGGTTTGGGTACT
ATTACCGACAGCCTCTCCGTTCTGAAAAAACATGTATTCGAAGAACGTAAATTCAATATGGAGCAAATCATCCATGCTAC
CGATACCAACTTCGAAGGACAGGAAGCCATGAGACAGTTCATACTGAACCGTACCCCTTTCTTCGGTAACGATGACGAAT
ATGCAGACCGCATCGCCATCCAGATCTTTAACGACCTTTACGATGCCATCGAGGGAAAGCCGAACACCAAAGGCGAATGT
TTCCATCTGAACATGTTGTCTACCACCTGTCACGTTTACTTCGGCAAAATGATGAATGCCACCCCCAACGGCCGTCTGGC
CGGACGTGCCATCAGTGACGGTACCTCACCCTCACACGGAGCAGATACCCACGGGCCATCCGCCGTTATAAAATCATTAG
GAAAACTGGACCAGGTGAAAAGCGGAGGCACTCTATTGAATCAGCGCTTCCTGCCTTCACTTCTTAAACACGATGAAGAC
ATAGCCAAACTGGCAAGCCTCATCCGCAGCTATTTTGCTTTAGGAGGACATCACATTCAGTTCAACATAGTAGATACCGC
TACCCTGCATGCCGCCCAAAAACATCCGGAAGAATACCGGGACCTATTGGTACGCGTAGCTGGATACAGTGATTACTTCA
ATGACATGAATACAGATTTACAATATGACGTAATAGCCCGCACGGCACAAGAAACCTTCTGA

Upstream 100 bases:

>100_bases
ATTTGATATCTTATTCTCTTGCACATCAATTGGTCGGATCCATTAATCCGCCCTTATGGAAATTTCTATTTATGAACCAG
TAGACCTTATTACAACTCTT

Downstream 100 bases:

>100_bases
CAAGATGAGTCTGATTTTCGACATCAAACGCTATGCCATCAATGACGGACCGGGCATCCGCATCACACTTTTCATGAAAG
GATGCCCGCTCTCGTGCATA

Product: formate C-acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MKTTINGMTDRIRRLRQESFDTQPSLSIERALIETEFYKENEGKYPIPILRALNFLEICKRKTIYIGEDELIVGERGPRP
KAVSTFPELTCHSVEDLHVLNTRELQRYTISQEDIDTYEREVIPYWKGRTQRERIFNHVPKEWKEAYEVGMFTEFMEQRA
PGHTSLDGKVYQHGMLDLKERIAHELKNLDFMNDPEATDKQEELTAMSISCDAAIIFAERHAELAEKMAGQETDPKRKEE
LKKIAEICRWVPAHAPRTYWEAIQMYWFVHLGTITELNGWDAMNPGHFDQHLAPFYEKDLEEGILTRAEAKELMSCFFIK
VNNQTAPPKVGITAKESGTYNDFTNLNIGGIKRDGSNGVSEVSYIMLETVDDLHLLQPGSALHISVCTPERFLREGCKVI
RKGYGYPSVFNPDTYVMELMRQGKTPEDAREGGCSGCIEVGAFGKEAYILTGYLNVPKILEVTLHNGTDPVSGKKVGLVT
GDPCTFRSYEALYDAFLKQIHYFVDMKVRVSNYIDRMFAKYAPATFLSLFIDDCIAKGKDYYNCGPRYNTSYIQCTGLGT
ITDSLSVLKKHVFEERKFNMEQIIHATDTNFEGQEAMRQFILNRTPFFGNDDEYADRIAIQIFNDLYDAIEGKPNTKGEC
FHLNMLSTTCHVYFGKMMNATPNGRLAGRAISDGTSPSHGADTHGPSAVIKSLGKLDQVKSGGTLLNQRFLPSLLKHDED
IAKLASLIRSYFALGGHHIQFNIVDTATLHAAQKHPEEYRDLLVRVAGYSDYFNDMNTDLQYDVIARTAQETF

Sequences:

>Translated_793_residues
MKTTINGMTDRIRRLRQESFDTQPSLSIERALIETEFYKENEGKYPIPILRALNFLEICKRKTIYIGEDELIVGERGPRP
KAVSTFPELTCHSVEDLHVLNTRELQRYTISQEDIDTYEREVIPYWKGRTQRERIFNHVPKEWKEAYEVGMFTEFMEQRA
PGHTSLDGKVYQHGMLDLKERIAHELKNLDFMNDPEATDKQEELTAMSISCDAAIIFAERHAELAEKMAGQETDPKRKEE
LKKIAEICRWVPAHAPRTYWEAIQMYWFVHLGTITELNGWDAMNPGHFDQHLAPFYEKDLEEGILTRAEAKELMSCFFIK
VNNQTAPPKVGITAKESGTYNDFTNLNIGGIKRDGSNGVSEVSYIMLETVDDLHLLQPGSALHISVCTPERFLREGCKVI
RKGYGYPSVFNPDTYVMELMRQGKTPEDAREGGCSGCIEVGAFGKEAYILTGYLNVPKILEVTLHNGTDPVSGKKVGLVT
GDPCTFRSYEALYDAFLKQIHYFVDMKVRVSNYIDRMFAKYAPATFLSLFIDDCIAKGKDYYNCGPRYNTSYIQCTGLGT
ITDSLSVLKKHVFEERKFNMEQIIHATDTNFEGQEAMRQFILNRTPFFGNDDEYADRIAIQIFNDLYDAIEGKPNTKGEC
FHLNMLSTTCHVYFGKMMNATPNGRLAGRAISDGTSPSHGADTHGPSAVIKSLGKLDQVKSGGTLLNQRFLPSLLKHDED
IAKLASLIRSYFALGGHHIQFNIVDTATLHAAQKHPEEYRDLLVRVAGYSDYFNDMNTDLQYDVIARTAQETF
>Mature_793_residues
MKTTINGMTDRIRRLRQESFDTQPSLSIERALIETEFYKENEGKYPIPILRALNFLEICKRKTIYIGEDELIVGERGPRP
KAVSTFPELTCHSVEDLHVLNTRELQRYTISQEDIDTYEREVIPYWKGRTQRERIFNHVPKEWKEAYEVGMFTEFMEQRA
PGHTSLDGKVYQHGMLDLKERIAHELKNLDFMNDPEATDKQEELTAMSISCDAAIIFAERHAELAEKMAGQETDPKRKEE
LKKIAEICRWVPAHAPRTYWEAIQMYWFVHLGTITELNGWDAMNPGHFDQHLAPFYEKDLEEGILTRAEAKELMSCFFIK
VNNQTAPPKVGITAKESGTYNDFTNLNIGGIKRDGSNGVSEVSYIMLETVDDLHLLQPGSALHISVCTPERFLREGCKVI
RKGYGYPSVFNPDTYVMELMRQGKTPEDAREGGCSGCIEVGAFGKEAYILTGYLNVPKILEVTLHNGTDPVSGKKVGLVT
GDPCTFRSYEALYDAFLKQIHYFVDMKVRVSNYIDRMFAKYAPATFLSLFIDDCIAKGKDYYNCGPRYNTSYIQCTGLGT
ITDSLSVLKKHVFEERKFNMEQIIHATDTNFEGQEAMRQFILNRTPFFGNDDEYADRIAIQIFNDLYDAIEGKPNTKGEC
FHLNMLSTTCHVYFGKMMNATPNGRLAGRAISDGTSPSHGADTHGPSAVIKSLGKLDQVKSGGTLLNQRFLPSLLKHDED
IAKLASLIRSYFALGGHHIQFNIVDTATLHAAQKHPEEYRDLLVRVAGYSDYFNDMNTDLQYDVIARTAQETF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=793, Percent_Identity=32.7868852459016, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1787044, Length=811, Percent_Identity=31.5659679408138, Blast_Score=381, Evalue=1e-106,
Organism=Escherichia coli, GI48994926, Length=541, Percent_Identity=25.6931608133087, Blast_Score=142, Evalue=1e-34,
Organism=Escherichia coli, GI1787131, Length=624, Percent_Identity=24.1987179487179, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 90201; Mature: 90201

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTINGMTDRIRRLRQESFDTQPSLSIERALIETEFYKENEGKYPIPILRALNFLEICK
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RKTIYIGEDELIVGERGPRPKAVSTFPELTCHSVEDLHVLNTRELQRYTISQEDIDTYER
CCCEEECCCCEEECCCCCCCCHHHHCCHHHHCCCCCEEECCHHHHHHHHCCHHHHHHHHH
EVIPYWKGRTQRERIFNHVPKEWKEAYEVGMFTEFMEQRAPGHTSLDGKVYQHGMLDLKE
HCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RIAHELKNLDFMNDPEATDKQEELTAMSISCDAAIIFAERHAELAEKMAGQETDPKRKEE
HHHHHHHCCCCCCCCCCCCCHHHHHEEEEECCEEEEEHHHHHHHHHHHCCCCCCHHHHHH
LKKIAEICRWVPAHAPRTYWEAIQMYWFVHLGTITELNGWDAMNPGHFDQHLAPFYEKDL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHH
EEGILTRAEAKELMSCFFIKVNNQTAPPKVGITAKESGTYNDFTNLNIGGIKRDGSNGVS
HHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCEECCCCCCHH
EVSYIMLETVDDLHLLQPGSALHISVCTPERFLREGCKVIRKGYGYPSVFNPDTYVMELM
HHHHHHHHHHCHHHEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RQGKTPEDAREGGCSGCIEVGAFGKEAYILTGYLNVPKILEVTLHNGTDPVSGKKVGLVT
HCCCCCCHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEE
GDPCTFRSYEALYDAFLKQIHYFVDMKVRVSNYIDRMFAKYAPATFLSLFIDDCIAKGKD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YYNCGPRYNTSYIQCTGLGTITDSLSVLKKHVFEERKFNMEQIIHATDTNFEGQEAMRQF
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHH
ILNRTPFFGNDDEYADRIAIQIFNDLYDAIEGKPNTKGECFHLNMLSTTCHVYFGKMMNA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCC
TPNGRLAGRAISDGTSPSHGADTHGPSAVIKSLGKLDQVKSGGTLLNQRFLPSLLKHDED
CCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHH
IAKLASLIRSYFALGGHHIQFNIVDTATLHAAQKHPEEYRDLLVRVAGYSDYFNDMNTDL
HHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCHHHHHHHHHHHHCCHHHHHCCCCCC
QYDVIARTAQETF
CHHHHHHHHHHCC
>Mature Secondary Structure
MKTTINGMTDRIRRLRQESFDTQPSLSIERALIETEFYKENEGKYPIPILRALNFLEICK
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
RKTIYIGEDELIVGERGPRPKAVSTFPELTCHSVEDLHVLNTRELQRYTISQEDIDTYER
CCCEEECCCCEEECCCCCCCCHHHHCCHHHHCCCCCEEECCHHHHHHHHCCHHHHHHHHH
EVIPYWKGRTQRERIFNHVPKEWKEAYEVGMFTEFMEQRAPGHTSLDGKVYQHGMLDLKE
HCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RIAHELKNLDFMNDPEATDKQEELTAMSISCDAAIIFAERHAELAEKMAGQETDPKRKEE
HHHHHHHCCCCCCCCCCCCCHHHHHEEEEECCEEEEEHHHHHHHHHHHCCCCCCHHHHHH
LKKIAEICRWVPAHAPRTYWEAIQMYWFVHLGTITELNGWDAMNPGHFDQHLAPFYEKDL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHH
EEGILTRAEAKELMSCFFIKVNNQTAPPKVGITAKESGTYNDFTNLNIGGIKRDGSNGVS
HHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCEECCCCCCHH
EVSYIMLETVDDLHLLQPGSALHISVCTPERFLREGCKVIRKGYGYPSVFNPDTYVMELM
HHHHHHHHHHCHHHEECCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RQGKTPEDAREGGCSGCIEVGAFGKEAYILTGYLNVPKILEVTLHNGTDPVSGKKVGLVT
HCCCCCCHHHHCCCCHHHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEE
GDPCTFRSYEALYDAFLKQIHYFVDMKVRVSNYIDRMFAKYAPATFLSLFIDDCIAKGKD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YYNCGPRYNTSYIQCTGLGTITDSLSVLKKHVFEERKFNMEQIIHATDTNFEGQEAMRQF
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHH
ILNRTPFFGNDDEYADRIAIQIFNDLYDAIEGKPNTKGECFHLNMLSTTCHVYFGKMMNA
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHCC
TPNGRLAGRAISDGTSPSHGADTHGPSAVIKSLGKLDQVKSGGTLLNQRFLPSLLKHDED
CCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHH
IAKLASLIRSYFALGGHHIQFNIVDTATLHAAQKHPEEYRDLLVRVAGYSDYFNDMNTDL
HHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHCHHHHHHHHHHHHCCHHHHHCCCCCC
QYDVIARTAQETF
CHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]